Ascc3 (NM_001277057) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Helic1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217751 representing NM_001277057 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTACCTCGCCTCACAGGAGCCTTGCGCTCCTTTTCAAATGTCACCAAGCAAGATAATTATAATG AAGAAGTAGCTGACTTAAAGCTGAAGCGATCCAAACTTCATGAACAAGCTTTAGATTTCGGCCTTTCATG GAAGAAGATAGTGAAGTTTTTGAATGAAAAACTAGAAAAGAATAAAATGCAAACTATAAATGAAGACTTA AAAGATATACTACAAGCTGCAAAACAAATCGTTGGAACGGATAATGGGAATGAAGCAATTGAAAGTGGGG CTGCATTCCTCTTTATGACGTTTCACATGACAGACTCTGTTGGTTACACGGAGACAAAGGCTATCAGACA GATGTTTGGACCCTTTCCATCATCCTCTGCTACCTCAGCTTGTAATGCCACTAGTCGGATTATTTCGCAT TTTAGTCAAGATGATCTCACTGCTTTAGTCCAGGCGACAGAAAACCAATGTAATGATAGAGTGGTCTTTG GTAGAAACTTAGCATTTTCCTTTGACATGCATGATCTGGACCACTTTGATGAATTACCAGTAAATGGTGA AGCCCAGAAAACTATAAGCTTAGACTACAAGAAGTTCCTGAATGAACAGTTTCAGGAACCCTACACCCCA GAACTCAAGCCCGTGGAAAAATCAAATGGCTCCTTATTGTGGTGTGAAGTAGAGAAGTACTTAAACGCAA CTCTAAAGGAAATGACTGAAGCACCAAGAGTTGAAGATCTGTGCTGTACGTTATATGACATGCTTGCTTC TGCTAAAAGCGGGGATGAGCTTCAGGATGAGCTTTTTGAATTGCTGGGACCTGGAGGACTTGATTTGATT GAGAAGCTACTCCAGAACAGAATTACAATCGTAGATCGATTTCTTAATTCCTCAAGTGATCATAAGTTCC AAGTTCTTCAAGATAATTGTAAAAAAATTTTAGGAGAGAATGCGAAGCCTAATTATGGTTGTCAAGTCAC GATTCAGTCCGAACAAGAAAAGCAGTTAATGAAACAGTATCGGCGTGAGGAAAAAAGAATTGCCAGGAGA GAAAAGAAGGCTGGGGAAGATGGGGAAGTTTCTGGAGAAGGACTTTTGCCCTTTGATCCTAAGGAACTCC GATTACAGAGAGAACATGCGCTTCTGAATGCTAGAAGTGCTCCAATTTTGGGAAGGCAGAGAGACACGGA GGTTGAGAAAATTCGTTATCCTCATGTTTATGATTCCCAGGCTGCAGCCCGAGAAACATCAGCATTTATA GCTGGGGCCAAGATGATTTTGCCAGAAGGCATCCAGAGAGAGAATACCAAGCTGTATGAAGAAGTAAGGA TCCCCTATAGTGAACCAATGCCCGTTGGGTTTGAGGAGAAACCAGTTTATATCCAGGATTTAGATGAGGT GGGACAGTTGGCTTTTAAAGGAATGAAGAGACTCAATAGAATCCAGTCTATAGTATTTGACACAGCCTAC AACACCAACGAAAACATGCTCATCTGTGCCCCTACTGGAGCTGGGAAGACCAATATTGCAATGCTCACTG TCTTACATGAAATCCGCCAACACTTTCACCAAGGTGTTCTTAAAAAGAATGAGTTTAAGATTGTGTATGT TGCTCCAATGAAAGCCTTGGCAGCTGAAATGACGAATTACTTCAGCAAACGTCTAGAGCCACTGGGCATT GTTGTGAAAGAATTGACTGGAGATATGCAGTTGTCAAAAAGTGAAATTCTACGAACTCAGATGCTTGTGA CCACACCAGAAAAATGGGATGTAGTAACAAGAAAGAGTGTTGGGGATGTTGCTCTTTCCCAAATTGTAAA ACTCCTGATTCTCGATGAGGTTCACTTGCTGCATGAAGACAGAGGCCCAGTGCTAGAAAGCATAGTTGCG AGGACCTTACGGCAGGTTGAATCCACACAGAGTATGATAAGAATCCTTGGCTTATCTGCAACCTTGCCCA ACTACCTGGATGTTGCTACATTTTTACATGTTAACCCCTACATTGGTCTTTTCTACTTTGATGGCCGTTT TCGACCTGTACCTCTGGGACAGACATTTTTAGGGATTAAAAGTGCAAATAAGATGCAGCAGTTAAATAAC ATGGATGAAGTGTGCTACGAAAGCGTTCTGAAGCAAGTAAAGGCTGGACACCAGGTGATGGTATTTGTAC ATGCTCGAAATGCCACTGTAAGGACAGCTATGTCTCTAATAGAGAGAGCAAAAAACAGTGGCCAGATCTC CTGTTTTCTCCCCACCCAAGGACCAGAGTATGGGCATGCATTGAAACAGGTACAAAAGTCAAGAAATAAG CAAGTGCGAGAGTTATTCTCAGATGGCTTTAGCATTCATCATGCCGGGATGCTTCGGCAAGACAGAAATT TGGTTGAAAATTTGTTCTCAAATGGGCACATCAAAGTCCTGGTTTGCACAGCCACCCTAGCCTGGGGTGT CAATCTCCCTGCACATGCCGTCATTATTAAGGGAACACAAATCTATGCTGCCAAAAGAGGCTCCTTTGTT GACCTTGGAATTTTAGATGTCATGCAGATATTTGGCCGAGCCGGACGACCGCAATTTGACAAATTTGGGG AAGGAATCATTATAACCACACATGATAAACTCAGCCATTACCTCAGTTTGCTCACTCAACAAAACCCGAT TGAGAGTCAGTTTCTGGAAAGTCTTGCAGATAACCTAAATGCAGAGATTGCACTAGGAACAGTCACCAAT GTGGAAGAGGCGGTGAAGTGGATGAGTTACACGTACCTCTACGTGCGGATGAGAGCAAACCCACTCGCCT ACGGCATCAGTCACAAAGCATACCAGATGGACCCAACGTTAAGAAAGCACCGAGAACAGCTCCTCATTGA AGTTGGACAAAAGCTGGATAAAGCTAGGATGATTCGCTTTGAAGAACGAACTGGCTATTTTTCTTCCACT GATTTGGGTAGAACTGCCAGCCACTACTATATTAAATACAACACCATTGAGACATTCAATGAACTCTTTG ATGCACACAAAACAGAAGGTGATATCTTTGCTATTGTCTCCAAAGCTGAAGAATTTGATCAAATTAAGGT CAGAGAAGAAGAAATAGAAGAATTAGATGCCTTGTTAAACAATTTCTGTGAACTCTCAGCTCCAGGAGGT GTGGAAAATAGTTATGGGAAAATAAACATCCTACTTCAGACCTACATCAGCCGAGGAGAGATGGACAGTT TCTCCCTCATATCAGATTCCGCATATGTTGCCCAGAATGCAGCTAGAATTGTCCGTGCTCTCTTTGAAAT TGCACTGAGGAAACGTTGGCCTACCATGACCTACAGGCTTTTGAATCTTAGTAAAGTCATTGACAAGAGA CTCTGGGGTTGGGCTAGCCCTTTGAGACAATTTTCTGTGTTACCACCACACATCCTAACAAGATTAGAAG AAAAAAACCTTACAGTGGATAAACTCAAAGACATGAGAAAAGATGAAATAGGTCACATTTTGCATCATGT GAATATTGGACTGAAGGTCAAACAGTGTGTTCATCAGATTCCTTCTGTTACAATGGAAGCTTCCATTCAG CCCATCACACGGACTGTCCTCCGAGTCTCACTGAACATCTACCCTGATTTCTCTTGGAATGATCAGGTCC ATGGCACAGTAGGGGAACCGTGGTGGATCTGGGTAGAAGATCCTACAAACGACCATATTTATCACTCGGA GTACTTTCTAGCTCTTAAAAAACAGGTTATTAATAAAGAAGCTCAACTTCTGGTATTTACAATCCCTATT TTTGAGCCTTTGCCCTCTCAGTACTACATTCGAGCAGTATCTGATAGATGGCTAGGAGCTGAAGCTGTTT GCATTATCAACTTTCAACATCTGATTCTACCAGAGAGACATCCTCCCCATACAGAATTATTGGACCTTCA GCCTTTGCCAGTCACAGCCTTGGGGTGCAAAGCATATGAAGCTCTGTATAACTTCAGCCACTTCAACCCG GTGCAGACACAGATATTCCACACGCTGTATCACACTGACTGTAACGTCCTACTTGGAGCCCCCACGGGGT CAGGAAAGACAGTTGCAGCTGAACTCGCCATTTTCAGAGTCTTCAACAAGTATCCCACTTCCAAGGCAGT ATATATTGCACCCCTCAAAGCTCTAGTACGTGAAAGAATGGATGATTGGAAAATTAGAATAGAAGAAAAA CTTGGTAAAAAAGTTATTGAACTGACTGGGGATGTGACTCCAGACATGAAATCCATTGCTAAGGCTGACC TTATCGTCACTACACCAGAAAAGTGGGATGGCGTCAGTCGAAGCTGGCAGAACAGGAGCTACGTTCAGCA GGTCAACATTCTCATCATAGATGAGATCCACCTGCTAGGTGAGGAGAGAGGTCCAGTTCTAGAAGTCATT GTATCTCGAACAAACTTTATCTCATCGCACACAGAGAAGCCTGTTAGAATAGTCGGACTGTCCACTGCCT TAGCCAATGCTAGAGACCTTGCTGACTGGCTCAACATTAAGCAGATGGGTTTGTTTAACTTCCGACCTTC TGTGCGCCCTGTTCCACTGGAAGTTCACATTCAAGGTTTCCCAGGACAACATTACTGCCCACGCATGGCT AGTATGAACAAGCCTGCATTCCAGGCAATTAGAAGTCATTCTCCAGCCAAACCTGTTTTGATATTTGTCT CCTCAAGACGTCAAACACGGCTTACGGCTTTAGAGTTGATAGCTTTCCTGGCCACTGAAGAAGACCCAAA GCAGTGGTTAAACATGGACGAGCAAGAGATGGAGAACATCATTGCAACAGTGCGAGATTCCAACCTTAAG CTGACTCTAGCTTTTGGAATAGGAATGCATCATGCTGGACTACATGAAAGAGACCGAAAAACTGTGGAAG AGCTGTTTGTGAACTGTAAAGTGCAGGTTCTCATTGCGACAAGCACGTTAGCATGGGGTGTAAACTTTCC AGCACACTTAGTCATTATTAAGGGAACTGAATACTACGATGGAAAGACAAGACGCTATGTGGATTTCCCC ATCACAGATGTACTGCAGATGATGGGGCGAGCTGGGAGACCTCAGTTTGATGACCAGGGCAAAGCTGTAA TTCTAGTTCATGATATAAAAAAGGATTTTTATAAAAAGTTTCTTTATGAACCTTTCCCAGTGGAATCGAG CTTATTAGGAGTGTTGTCTGACCACTTAAATGCAGAGATTGCGGGAGGTACAATAACATCAAAGCAAGAT GCCTTGGATTATATCACTTGGACTTACTTTTTCCGACGTCTTATCATGAATCCCAGCTACTACAACTTGG GGGACGTCAGCCAGGACGCCATAAACAAGTTCCTGTCACATCTGATTGGGCAATCTCTGGTTGAGTTGGA GCTTTCCCACTGCATTGAAGTTGGAGAGGACAATCGCAGCATTGAACCACTGACATGTGGCCGAATTGCC TCCTATTACTATCTGAAGCACAAGACAGTCAAAATGTTCAAGGACCGTTTAAAGCCTGAGTGCAGCACTG AAGAATTGCTGTCAATTCTAAGTGATGCGGAAGAATACACAGACTTGCCAGTAAGACACAATGAGGACCA TACGAATAATGAACTGGCCAAATGTCTTCCCATTGAATTAAATCCTCATTCATTTGACAGCCCTCACACC AAAGCCCATCTCTTGCTTCAAGCACATCTCAGCCGAGCCATGCTGCCCTGCCCAGACTATGATACTGATA CCAAAACAGTCTTAGATCAAGCTCTCAGAGTATGCCAGGCAATGCTGGATGTGGCTGCAAGCCAAGGATG GCTAGTGACTACCCTGAATATCACCCACCTGATTCAGATGGTGATCCAAGGCCGATGGCTAAAGGACTCG TCTCTTCTTACCATACCAAACATAGAACAGCACCATCTTCACCTTTTCAGGAAGTGGAAACCACCTGTAA AGGGCCCACATGCTAAGTGCCGAACATCCATTGAGTGCCTTCCTGAGCTGATCCATGCCTGTGAAGGGAA GGAGCATGTGTTCAGCTCCATGGTAGAGAAGGAGTTACAGCCTGCGAAAACAAAGCAGGCATGGAATTTC TTATCTCACTTGCCCGTGATAAATGTTGGCATAAGTGTTAAAGGCTCCTGGGATGACTCAGTTGAAGGAC ATAATGAACTCTCCATCTCAACTCTGACAGCAGACAAACGGGATGAAAACACATGGATCAAGTTGCATGC TGATCAGCAGTATGTGCTTCAAGTCAGCCTACAGAGAGTCCATTTTGAGTTCCACAAGGTAAAACATGAG AGTCACGCAGTTACTCCTCGATTTCCAAAATTAAAAGATGAAGGGTGGTTTTTGATATTAGGAGAAGTGG ATAAGAGAGAGCTGGTGGCTGTAAAACGCGTAGGATTTGTTCGAACTCATCACGAGGCTTCCATCTCTTT CTTTACTCCGGAAGCACCTGGAAGATATATCTTTACCCTGTATCTCATGAGTGACTGTTACCTCGGCCTA GACCAGCAGTATGACATCTTTCTCAATGTCACAAAAGCAGACATCTCCACCCAGATCAACACAGAAGTCC CTGATGTCTCAACTGACTTGGCA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217751 representing NM_001277057 Red=Cloning site Green=Tags(s) MALPRLTGALRSFSNVTKQDNYNEEVADLKLKRSKLHEQALDFGLSWKKIVKFLNEKLEKNKMQTINEDL KDILQAAKQIVGTDNGNEAIESGAAFLFMTFHMTDSVGYTETKAIRQMFGPFPSSSATSACNATSRIISH FSQDDLTALVQATENQCNDRVVFGRNLAFSFDMHDLDHFDELPVNGEAQKTISLDYKKFLNEQFQEPYTP ELKPVEKSNGSLLWCEVEKYLNATLKEMTEAPRVEDLCCTLYDMLASAKSGDELQDELFELLGPGGLDLI EKLLQNRITIVDRFLNSSSDHKFQVLQDNCKKILGENAKPNYGCQVTIQSEQEKQLMKQYRREEKRIARR EKKAGEDGEVSGEGLLPFDPKELRLQREHALLNARSAPILGRQRDTEVEKIRYPHVYDSQAAARETSAFI AGAKMILPEGIQRENTKLYEEVRIPYSEPMPVGFEEKPVYIQDLDEVGQLAFKGMKRLNRIQSIVFDTAY NTNENMLICAPTGAGKTNIAMLTVLHEIRQHFHQGVLKKNEFKIVYVAPMKALAAEMTNYFSKRLEPLGI VVKELTGDMQLSKSEILRTQMLVTTPEKWDVVTRKSVGDVALSQIVKLLILDEVHLLHEDRGPVLESIVA RTLRQVESTQSMIRILGLSATLPNYLDVATFLHVNPYIGLFYFDGRFRPVPLGQTFLGIKSANKMQQLNN MDEVCYESVLKQVKAGHQVMVFVHARNATVRTAMSLIERAKNSGQISCFLPTQGPEYGHALKQVQKSRNK QVRELFSDGFSIHHAGMLRQDRNLVENLFSNGHIKVLVCTATLAWGVNLPAHAVIIKGTQIYAAKRGSFV DLGILDVMQIFGRAGRPQFDKFGEGIIITTHDKLSHYLSLLTQQNPIESQFLESLADNLNAEIALGTVTN VEEAVKWMSYTYLYVRMRANPLAYGISHKAYQMDPTLRKHREQLLIEVGQKLDKARMIRFEERTGYFSST DLGRTASHYYIKYNTIETFNELFDAHKTEGDIFAIVSKAEEFDQIKVREEEIEELDALLNNFCELSAPGG VENSYGKINILLQTYISRGEMDSFSLISDSAYVAQNAARIVRALFEIALRKRWPTMTYRLLNLSKVIDKR LWGWASPLRQFSVLPPHILTRLEEKNLTVDKLKDMRKDEIGHILHHVNIGLKVKQCVHQIPSVTMEASIQ PITRTVLRVSLNIYPDFSWNDQVHGTVGEPWWIWVEDPTNDHIYHSEYFLALKKQVINKEAQLLVFTIPI FEPLPSQYYIRAVSDRWLGAEAVCIINFQHLILPERHPPHTELLDLQPLPVTALGCKAYEALYNFSHFNP VQTQIFHTLYHTDCNVLLGAPTGSGKTVAAELAIFRVFNKYPTSKAVYIAPLKALVRERMDDWKIRIEEK LGKKVIELTGDVTPDMKSIAKADLIVTTPEKWDGVSRSWQNRSYVQQVNILIIDEIHLLGEERGPVLEVI VSRTNFISSHTEKPVRIVGLSTALANARDLADWLNIKQMGLFNFRPSVRPVPLEVHIQGFPGQHYCPRMA SMNKPAFQAIRSHSPAKPVLIFVSSRRQTRLTALELIAFLATEEDPKQWLNMDEQEMENIIATVRDSNLK LTLAFGIGMHHAGLHERDRKTVEELFVNCKVQVLIATSTLAWGVNFPAHLVIIKGTEYYDGKTRRYVDFP ITDVLQMMGRAGRPQFDDQGKAVILVHDIKKDFYKKFLYEPFPVESSLLGVLSDHLNAEIAGGTITSKQD ALDYITWTYFFRRLIMNPSYYNLGDVSQDAINKFLSHLIGQSLVELELSHCIEVGEDNRSIEPLTCGRIA SYYYLKHKTVKMFKDRLKPECSTEELLSILSDAEEYTDLPVRHNEDHTNNELAKCLPIELNPHSFDSPHT KAHLLLQAHLSRAMLPCPDYDTDTKTVLDQALRVCQAMLDVAASQGWLVTTLNITHLIQMVIQGRWLKDS SLLTIPNIEQHHLHLFRKWKPPVKGPHAKCRTSIECLPELIHACEGKEHVFSSMVEKELQPAKTKQAWNF LSHLPVINVGISVKGSWDDSVEGHNELSISTLTADKRDENTWIKLHADQQYVLQVSLQRVHFEFHKVKHE SHAVTPRFPKLKDEGWFLILGEVDKRELVAVKRVGFVRTHHEASISFFTPEAPGRYIFTLYLMSDCYLGL DQQYDIFLNVTKADISTQINTEVPDVSTDLA SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277057
ORF Size	6603 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277057.1, NP_001263986.1
RefSeq Size	7446 bp
RefSeq ORF	6606 bp
Locus ID	309887
MW	250.5 kDa

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