Frem1 (NM_001191757) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	RGD1306981
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217752 representing NM_001191757 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCTCCAGGCTGCACTGGGCCCAAGGCTCTGTTGTTCTTGCTGCTCCAGCTGCTTATGCTACACC TGGACCCAGCCAATGCCACCTTCATCAGCATTAACCGGGGCCTGAGGGTGATGAAGGGTAGTTCTGCCTT CCTGTCTGGAGATCACCTGAGGTTTGCCATCCCTAAAGAGAAGGACGCTTGCAGAGTGGAGGTGGTGATG AATGAACCCATAACCCAGAGGGTTGGGAAACTCATTCCACAGGTCTTTGATTGCCATTTTCTTCCCAACG AAGTACAATATGTTCACAATGGATGCCCAATTCTGGACGAAGACTCTGTGAAGCTTAGACTTTACAGGTT TACTGAAACAGAGACTTTCATGGAAACCTTCCTCTTGCGGGTCTCTCTTGTGGAACCAGACTGTAACATC ATCCGCCTGAGCAGCAACGTGTTGGAAGTGACTGAATTCTATGGCTTATCCCAAGCCATTGATAAGAACC TGCTCCAGTTTGATTATGATAGGACAGCCAGTCTGGACTGCACCATCAGAGTAGACCCCGTGAAGACTCA GCTGCCAGCCCATGGCAAGCTGGTTGTGGTGAATCGGAAGTCAGAGGGACCGCGTGGAGACCAGCCACAC AGCTTTTTCTCTGAGTCACAGCTAGGGGCAGAACGGAAGTGTCCGGATGGAAGCTGTACTCTGGAACCGA AGCAAGTGACAAGTCTGAAAGTGAGCTGTGAGGACTTCCTGCTGACAGGGTTTCACTACCAACACATGCA GCCCCCTTCACCCAACATTGACTACATCCCCATTCAGTTGGATCTCACAGACAGAAGGAGTAAAACCATT TACAAGTCAGAGAGCGCGTGGTTGCCTGTCTTCATCAGAGTTGGCATTCCTAACCAGGCCCCAAGGGCTG CTTTCATGGCCATGTTTGTTCTGGAGGTGGACCAGTTCATATTGACCCCTCTGACAACCTCCGTGCTGGA CTGTGAGGAGGATGAGACTCCGAAACCCTTGCTGGTGTTTAACATCACTGAAGCCCCACTCCAGGGCTAT GTGACCCACCTGCTGGATCATACCAGACCCATCTCTTCATTTACCTGGAAGGATCTCAGTGACATGCAAG TTGCCTACCAGCCACCGAACAACAGCCACCCTGAGAGGAGACATTACAAGATGGAGCTGGAGGTGTACGA CCTCTTCTTTGAGAGGAGCGCCCCCATCACAGTTCACATCTCCATCAGAACAGCAGACACGAATGCACCT CGGGTGTCCTGGAATACAGGTTTGAATCTCCTAGAGGGGCAGTCTCGGGCCATCACTTGGGAACAGTTTC AGATCGTGGACAATGATGACATTGGTGCTGTGCAGTTGGTCACCATTGGTGGTCTGCAGCATGGACGGCT TACAGTAAGAGAGGGGAAAGGATTTCTCTTCACCGTGGCTGATCTTCAGGCTGGAGTTGTTCGCTATCAT CATGATGACAGTGATACCACCAAAGACTTTGTGGCTTTCAGGATATTCGATGGCCAACACAGCAGCCATC ACAAGTTTCCTATCAACATCTTGCCTAAAGATGACAGTCCCCCATTCCTCATAACCAATGTCGTGATTGA ACTAGAAGAGGGGAAGACCATCTTGATCCAAGGGTCCATGCTGAGAGCTTCAGACATGGACTCCAGTGAT GACTACATCTTCTTCAATATCACAAAGTTCCCACAGGCTGGGGAGATCATGAAGAAGCCAGGGCCAAGAT TGATAGGATATCCTGTCTCTGGCTTCCTTCAGAGAGACCTGTTCAGTGGAATCATTTACTATCGTCACTT TGGTGGAGAAATCTTTGAAGATTCTTTTGAATTTGTCCTGTGGGACAGCCATGAACCTCCAAATCTCTCA GTGCCACAGGTGGTGATGGTCCATATAACTCCAGTAGACGACCAGCTTCCCAAGGAAGCTCCCGGCATAT CCCGGCATTTGATTGTCAAGGAAACGGAAGTGGCCTACATCACTAAAAAGCACCTACATTTCCTAGACAC GGAATCGCATGGTGGGGAGCTTGTCTACACAATAACACGCCCTCCATGCTTCTCCTCCAGCCACAGGCAC TTGGATGCTGGGAAGCTGTTCATGGTGGACAGCATACCAAAGCCAACCAAGAATCCCACAGCCCCAGGGC TGAACTCATTTACTCAGGTAAAAAGATTTGGAAAGACCCTGGCTAACACAGTAAGCACATTGTTGGGGGG CTTGGGCTTGGGTTGCTCCAACATTGTGATCTCTGTAAATCAGTGCATATGTGCATCTCAATTTGCTTCA CAGCATGCTGTGAACCACATGAAAGTGGCCTACATGCCACCCATGCAGGATATAGGTCCCACACCTCGAC ATGTGCAGTTCACAGTGTCCGTCAGTAACCAGCATGGAGGTACTTTGCATGGAATCTGCTTCAACATCAC AGTGCTCCCAGTGGACAATCAGGTTCCTGAGGTATTCACCAACACCCTGAGAGTGGCTGAGGGTGGCCAA TGCACCATCAGCACAGAGCACATTTTGGTTTCTGATGTGGACACCCCACTGGACAGCATCTGCCTCTCTC TTCAGGAGATGCCCCTGCATGGCGGGGTAGAGCTGGATGGATTTCCTCTAAACCCAAGAGGCACATTTTC TTGGCGAGACCTCCATACCTTAAAAGTTAGGTATCAACATGATGGATCTGAGGTTCTTCAGGATGAGATA TTCTTGGAGGTCACAGACGGCACAAATTCTGCAGGATTTGTTCTCTACATTGAGGTATTCCCTGTGAATG ATGAGCCACCTGTTCTAAAGGCTGACCTCATTCCCATGATGCACTGCTCAGAGGGTGGAGAGGTGACCAT CACCCCTGAATACATTTTCGCTACTGATGCAGACAGCAATGACTCAGAACTGCTATTTCTGATTACCCGA GAACCTCGGCATGGAGTAGTGAGAAAAGCTGGACTCCGTGTGGACAGGTTCTCTCAGGGAGATGTCATCT CAGGAGCTGTGGCATACAAACATACAGGTGGCGAGATTGGCCTCGAATCTTGTTTTGACACTGTTGTATT GGTGGTTTCGGATGGTGAAGCTGACCCTTTGGTGAATGGCTGTTGCTACAATGGACCCGATTCATCTGTT CCTCTTCATAAGTCCTTCCCCACATACCAGCTCAACATCACGGTGCATCCAGTCGACAATCAGCCACCTT CAATTATAATAGGTAGGACGCTCACGGTGGACGAAGGCTTCTCGGCAGCCCTTACGACTCATCACTTGAC TGCCACTGACTGGGACACTGCTCCGGATGACTTGGAATTCATTTTGGTTTCTCATCCCCAGTTTGGCTAC CTAGAAAACACACTGCCCTCTGCAGGCTTTGAGAAAAGGAATACGGGCATAAGCATAGCTTCATTCCAGT GGAGAGACATGAAAGCTTCGCATATTAACTATGTGCAGTCCAGGCACCTGAGGGAGGAGCCAACTGCTGA TGAGTTCCTGGTGTACGCCACAGATGGGAAGCAACGCTCCTTGGAGACCACATTTCACGTTATCATCAAC CCCACAAATGATGAAGCTCCTGATTTTGTGGTACAGAATATCACTGTGTACGAGGGCCACATGGTAGAGC TGGATTCTGCCATCATCAGTGCTACTGACAGGGATGTTCCCAAGGACCCCTTGCTCTTCAACATCACGCA CAAGCCCAAGCATGGCCTCCTCCTCATCTCGGCCACCAGCAAGGATGCTCATAATGAAATTCACAACTTC TCCATGGAACTGCTCCAGAATGGAATGAAGCTGGCGTATGCCCACGATGACTCGGAGAGCTCTGCTGACA ACTTCGTGATCCAGTTATCAGACGGGAAACATAAAATCCTTAAAACCGTTTCAGTAAGCATCACCCCAGT GAACGACGAGACACCAACACTAAGCAAGAAGGCTGAAGTCTCAGTGACTGTGGGCGACACTCGTGTTCTG TCTAGTGCTGTTCTTTCAGCCATAGATAAAGACTCACCCAGGGAGAAGATTCACTACTTATTTGAAAGAC TTCCCCAAAATGGGCAACTTCAACTTAAGATAGGGAGGGACTGGGTCCCTCTCTCCGCTGGCATGCAGTG CACCCAGGAGGATGTGGACTTGAACTTGCTAAGATACGCGCATGCTGGTAAAATGCATTCCCAGGATGGA GACCGCTTTACCTTCTACCTCTGGGACGGGGACAACAGGTCACCTGCATTTGACTGTCACATCATCATTG AAGACACAGGAAGAGGTGATATTGTCATTCATGCGAAACCACTCGTGGTAGCCAACGGTGACAGAGGTCT CTTGACGCCTGCCACTCTTCTGGCTGAGGATGGAGCAGACAAGGCTGAGGAATTACTCTACCTTATCACT TCCCCACCACAGTATGGCCAAGTGGAGTATGTCTACTCTCCTGGAGTCCCCATTACCAGCTTTAGCCAAA TGGACATAGCAGGACAGGCAGTCTGCTACATACACAAGAGCAGGACCGCTGTCCTCAGTGACAGTTTCAG ATTTACCATCAGCAATGGACTGCAGACCCAGCAGGGGGTGTTTGAGATCACACTGCAGGGTGTGGACAGT GCCTTGCCTGTGCTGACCAGGAACCGAAGGCTGAGGCTTGTGGAAGGGGCCATGGGCCTGCTGTCCACGG ATCACCTTCAGCTGACTGACCCTGACACACCTCCAGAGAACCTAACCTTCTTTCTGGTCCAGCTCCCGCG CCACGGGTACCTCTACCTGAGAGGGAAAGTACTTCAGCACAACTTCACCCAGCAAGATGTAGACAGCAGG GTTGTGGCCTACCAGCATGCAGGAGGCGGTGCCCAGGAGGACTGCTTTACTTTCCTGGCCACAGACAGGA AGAACCAAGGCTTCGTTGTGGATGGGAAAGTTCAGAAAGAGCCCGTTCTCTTCATTATCCAGGTGGACCA GCTGGATAAGAAGGCGCCTCGGATCACACGCTTGCACTCCCCTTCTCAAGTGGGGCTCTTGAAAAATGGC TGCTATGGGATTTACATCACTTCCCATGTGCTAAAGGCGTCTGACCCTGACACAGAGGATGACCAGATCA TCTTTAAGATTTTACGAGGCCCGTTGCATGGACGTCTGGAGAACACGACAACAGGTGAATTCATCCACGA GCAATTTAGCCAGAAGGACTTGAGCCGCAAGACCATCCTTTATATCATAAACCCTTCTTTGCAAGTGAAT TCAGATATCCTGGAATTTCAGGCCATGGACCCCACAGGGAACACTGCCCCTCCCCAGAGATTGGAACTGA AATGGTCATATATTGAATGGTCCCAGACTGAATATGAGGTCTGTGAGAATGTGGGCTTACTGACCTTGGA AGTCACCAGAAGGGGATATTCCATGGACTCAGCCTATGTGGACATAGAGGTTAACCAAGTGTCAGCTGTA GTTGGAAAAGATTTTACTGTCACTCCATCCAAACTGCTTCAATTTGACCCAGGAATGTCAACTAAGATGT GGAATATAGCAATTACCTATGACGGATTAGAGGAAGACGATGAGGTCTTTGAAGTAATTCTGAACTCCCC TGTGAATGCGGTTCTTGGCACACAGACAAAAGCAGCAGTGAAAATTTTGGACTCAAAAGGAGGACGGTGC CATCCTTCAAATTCCTTCAACCAAAGCAAGCACAGCACGTGGGGGAAGGGACGTTGGCATCCACTGCCCT CAGGGTCATCCTCACTCACCACTTCCGGTTCTCCTCATCTGGAAAGAAGGCCCCCTCCATCCATCACCAA AGGAGATGCTCTTCGGGGTTTTGATCTCACAGACCTCTCTCAAAGGAAGATGACGACACAGGGGAATGGC AAATCAGTCCTCCCATCCTCTGTTTGTAGAAATGGAACAGACACCATCTACAATTATCATGGTATAGTTT CCTTGAAACTGGAGGGGGACAGATTCTCAGCTCACAAACGGAAGGCCAAAATATCCGTCATTCGTCAGCC ACAGAGGACAGTCAAGGTGGCAGAGCTGCCTCTAGCCGATAAGGTAGAACCCACAACCGACTCGAACTTT CTCAGTCAGGGTCTACGGCCCTTGTTTCCAAAGAACTGTTCTGTGGAATTAAAGGGACTCTTTCATTTGG AAGAACACACCCACAGATTGTATCAATGTGATGGGATCGCCTGGAACGTCTGGAGCTCCCAAACCAAGGG GCTGGAGGACAGATCCTGCCCAGTTGGATGGCTTCTTCATTCCGACTACTGTCACGTCTTGGTCACAAGG CAGAAAGGCACCTGGGCCACAGCTACCCGAGCTTGCAGAGAACAGCACCAGGGCACCCTTGTGACCGTAT TCTCCAGGAGGCACATGCAGTGGCTGTGGGCTATCAGCGGAAGAAAGCCCTTCTGGATAGGCTTGAAGAA CCAAGTGCGAACTGGCCACTGGGAGTGGATTGGAGGTGAACCTGTTGCTTTCACCAACTGGAGGAGAGGG CCCCCTCTGCACCCGAAGCCTGGAAAGAACTGTGTTTTGGTTCAAAGAAGAGGACAATGGCAAACAAAGA ACTGTAGCAAAGGCAAGGCTCACAATTTTGTGTGCTCAAGGAAACTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217752 representing NM_001191757 Red=Cloning site Green=Tags(s) MHSPGCTGPKALLFLLLQLLMLHLDPANATFISINRGLRVMKGSSAFLSGDHLRFAIPKEKDACRVEVVM NEPITQRVGKLIPQVFDCHFLPNEVQYVHNGCPILDEDSVKLRLYRFTETETFMETFLLRVSLVEPDCNI IRLSSNVLEVTEFYGLSQAIDKNLLQFDYDRTASLDCTIRVDPVKTQLPAHGKLVVVNRKSEGPRGDQPH SFFSESQLGAERKCPDGSCTLEPKQVTSLKVSCEDFLLTGFHYQHMQPPSPNIDYIPIQLDLTDRRSKTI YKSESAWLPVFIRVGIPNQAPRAAFMAMFVLEVDQFILTPLTTSVLDCEEDETPKPLLVFNITEAPLQGY VTHLLDHTRPISSFTWKDLSDMQVAYQPPNNSHPERRHYKMELEVYDLFFERSAPITVHISIRTADTNAP RVSWNTGLNLLEGQSRAITWEQFQIVDNDDIGAVQLVTIGGLQHGRLTVREGKGFLFTVADLQAGVVRYH HDDSDTTKDFVAFRIFDGQHSSHHKFPINILPKDDSPPFLITNVVIELEEGKTILIQGSMLRASDMDSSD DYIFFNITKFPQAGEIMKKPGPRLIGYPVSGFLQRDLFSGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLS VPQVVMVHITPVDDQLPKEAPGISRHLIVKETEVAYITKKHLHFLDTESHGGELVYTITRPPCFSSSHRH LDAGKLFMVDSIPKPTKNPTAPGLNSFTQVKRFGKTLANTVSTLLGGLGLGCSNIVISVNQCICASQFAS QHAVNHMKVAYMPPMQDIGPTPRHVQFTVSVSNQHGGTLHGICFNITVLPVDNQVPEVFTNTLRVAEGGQ CTISTEHILVSDVDTPLDSICLSLQEMPLHGGVELDGFPLNPRGTFSWRDLHTLKVRYQHDGSEVLQDEI FLEVTDGTNSAGFVLYIEVFPVNDEPPVLKADLIPMMHCSEGGEVTITPEYIFATDADSNDSELLFLITR EPRHGVVRKAGLRVDRFSQGDVISGAVAYKHTGGEIGLESCFDTVVLVVSDGEADPLVNGCCYNGPDSSV PLHKSFPTYQLNITVHPVDNQPPSIIIGRTLTVDEGFSAALTTHHLTATDWDTAPDDLEFILVSHPQFGY LENTLPSAGFEKRNTGISIASFQWRDMKASHINYVQSRHLREEPTADEFLVYATDGKQRSLETTFHVIIN PTNDEAPDFVVQNITVYEGHMVELDSAIISATDRDVPKDPLLFNITHKPKHGLLLISATSKDAHNEIHNF SMELLQNGMKLAYAHDDSESSADNFVIQLSDGKHKILKTVSVSITPVNDETPTLSKKAEVSVTVGDTRVL SSAVLSAIDKDSPREKIHYLFERLPQNGQLQLKIGRDWVPLSAGMQCTQEDVDLNLLRYAHAGKMHSQDG DRFTFYLWDGDNRSPAFDCHIIIEDTGRGDIVIHAKPLVVANGDRGLLTPATLLAEDGADKAEELLYLIT SPPQYGQVEYVYSPGVPITSFSQMDIAGQAVCYIHKSRTAVLSDSFRFTISNGLQTQQGVFEITLQGVDS ALPVLTRNRRLRLVEGAMGLLSTDHLQLTDPDTPPENLTFFLVQLPRHGYLYLRGKVLQHNFTQQDVDSR VVAYQHAGGGAQEDCFTFLATDRKNQGFVVDGKVQKEPVLFIIQVDQLDKKAPRITRLHSPSQVGLLKNG CYGIYITSHVLKASDPDTEDDQIIFKILRGPLHGRLENTTTGEFIHEQFSQKDLSRKTILYIINPSLQVN SDILEFQAMDPTGNTAPPQRLELKWSYIEWSQTEYEVCENVGLLTLEVTRRGYSMDSAYVDIEVNQVSAV VGKDFTVTPSKLLQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTQTKAAVKILDSKGGRC HPSNSFNQSKHSTWGKGRWHPLPSGSSSLTTSGSPHLERRPPPSITKGDALRGFDLTDLSQRKMTTQGNG KSVLPSSVCRNGTDTIYNYHGIVSLKLEGDRFSAHKRKAKISVIRQPQRTVKVAELPLADKVEPTTDSNF LSQGLRPLFPKNCSVELKGLFHLEEHTHRLYQCDGIAWNVWSSQTKGLEDRSCPVGWLLHSDYCHVLVTR QKGTWATATRACREQHQGTLVTVFSRRHMQWLWAISGRKPFWIGLKNQVRTGHWEWIGGEPVAFTNWRRG PPLHPKPGKNCVLVQRRGQWQTKNCSKGKAHNFVCSRKL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191757
ORF Size	6627 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191757.1, NP_001178686.1
RefSeq Size	6630 bp
RefSeq ORF	6630 bp
Locus ID	298185
MW	246.9 kDa

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