Prpf8 (NM_001191590) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR217763 representing NM_001191590 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGGAGTGTTTCCTTACAGAGGGCCGGGTAACCCGGTGCCCGGCCCTCTAGCCCCGCTGCCGGATT ACATGTCAGAGGAGAAGCTGCAGGAGAAAGCTCGAAAATGGCAGCAGTTGCAGGCCAAGCGCTATGCAGA GAAGCGGAAATTCGGGTTTGTGGATGCTCAGAAGGAAGACATGCCCCCAGAACATGTCAGGAAAATCATT CGAGACCATGGAGACATGACCAACAGGAAGTTCCGTCATGACAAAAGGGTCTATTTGGGAGCTCTCAAGT ACATGCCTCACGCAGTTCTCAAGCTCCTAGAGAACATGCCCATGCCTTGGGAGCAGATCCGGGATGTGCC TGTGCTGTACCACATCACTGGAGCCATTTCCTTCGTTAACGAGATTCCCTGGGTTATTGAGCCAGTTTAC ATCTCCCAGTGGGGGTCAATGTGGATCATGATGCGTAGAGAAAAAAGAGACAGGAGGCATTTCAAGAGGA TGCGTTTTCCCCCTTTTGATGATGAGGAGCCGCCCCTGGATTATGCTGACAACATCTTAGATGTCGAGCC ACTGGAAGCCATTCAGCTAGAGCTGGACCCTGAGGAGGATGCCCCTGTGTTGGACTGGTTCTATGACCAC CAGCCATTGAGAGATAGTAGGAAGTATGTTAATGGTTCCACCTACCAACGCTGGCAGTTCACATTGCCTA TGATGTCCACTCTCTACCGACTGGCCAACCAGCTTCTGACTGACTTGGTAGATGACAACTACTTTTATCT CTTTGATCTAAAGGCCTTCTTTACCTCTAAGGCACTCAACATGGCCATTCCTGGGGGACCCAAATTTGAA CCTCTTGTTCGAGACATCAACCTACAGGATGAAGACTGGAATGAATTCAATGATATTAACAAGATCATCA TCCGTCAGCCTATTCGTACTGAGTACAAGATTGCTTTTCCGTACCTCTATAACAACCTCCCACACCATGT CCACCTGACCTGGTACCACACTCCTAACGTTGTGTTCATCAAGACTGAGGATCCTGATCTGCCAGCTTTC TACTTTGATCCTCTGATTAACCCAATCTCCCATAGACACTCAGTCAAGAGCCAAGAGCCACTACCTGATG ATGATGAGGAGTTTGAGCTCCCAGAGTTTGTGGAGCCCTTCCTGAAGGATACACCTCTCTATACAGACAA TACAGCCAATGGCATCGCACTGCTCTGGGCACCACGGCCCTTCAACCTACGCTCTGGTCGTACCCGCCGG GCCCTCGACATTCCCCTTGTCAAGAACTGGTATCGGGAGCACTGTCCTGCTGGGCAACCTGTGAAAGTTC GAGTCTCCTATCAGAAGCTGCTTAAGTATTATGTGCTGAATGCCCTCAAACATCGGCCTCCCAAAGCGCA GAAGAAGAGGTATCTGTTTCGCTCTTTCAAAGCTACCAAATTCTTTCAGTCTACCAAGCTGGACTGGGTG GAGGTCGGGCTGCAGGTTTGCCGCCAGGGCTACAACATGCTTAACCTTCTCATTCACCGTAAGAACCTCA ACTATCTGCATTTGGACTATAACTTCAACCTGAAGCCTGTCAAGACGCTCACCACCAAGGAAAGAAAAAA ATCTCGCTTTGGGAATGCTTTCCATCTGTGTCGGGAAGTACTGCGTTTGACTAAGCTGGTTGTGGATAGT CATGTGCAGTATCGCTTGGGCAATGTAGATGCCTTCCAGCTGGCAGATGGATTGCAGTATATATTTGCCC ACGTGGGACAGTTGACAGGCATGTATCGATACAAATATAAGCTGATGCGGCAGATCCGCATGTGCAAGGA CTTGAAACATCTCATCTATTACCGCTTCAACACGGGTCCTGTGGGGAAAGGCCCTGGCTGTGGTTTCTGG GCTGCTGGCTGGCGGGTCTGGCTATTTTTTATGCGTGGAATTACCCCTTTGCTAGAACGATGGCTAGGCA ACCTTCTGGCCCGACAGTTTGAAGGCCGTCACTCAAAGGGGGTGGCAAAGACAGTAACAAAGCAACGAGT AGAGTCCCATTTTGACCTTGAGCTTAGGGCAGCTGTGATGCATGATATTCTGGACATGATGCCTGAAGGC ATCAAACAGAACAAGGCCCGAACAATTCTGCAGCACCTCAGTGAGGCCTGGCGCTGCTGGAAGGCCAACA TTCCATGGAAGGTCCCAGGACTGCCAACGCCTATAGAGAATATGATTCTTCGGTACGTTAAGGCCAAGGC TGATTGGTGGACCAATACTGCCCACTACAACCGTGAACGGATTCGCCGTGGGGCTACTGTAGACAAGACT GTTTGCAAAAAGAACCTGGGCCGCCTCACCCGCCTGTATCTGAAGGCAGAACAGGAGCGGCAGCACAATT ACCTGAAGGACGGGCCTTACATCACAGCAGAGGAAGCAGTGGCAGTGTATACTACCACTGTGCACTGGCT GGAAAGCCGTAGGTTCTCTCCTATCCCGTTCCCCCCACTCTCCTACAAGCATGACACCAAGTTGCTTATC TTGGCTCTGGAGCGACTCAAGGAAGCTTATAGTGTGAAGTCTAGGTTAAACCAGTCTCAGAGGGAGGAGC TGGGTCTGATTGAACAAGCCTATGACAACCCCCATGAGGCTTTGTCCCGCATCAAGCGTCACCTCCTCAC GCAGAGAGCCTTTAAGGAAGTGGGCATTGAGTTTATGGATCTCTATAGCCACCTTGTGCCAGTGTATGAT GTGGAACCACTGGAGAAAATAACTGATGCTTACCTGGACCAGTACCTGTGGTATGAAGCAGATAAACGCC GTCTTTTCCCGCCCTGGATCAAGCCTGCTGACACAGAACCCCCTCCACTGCTTGTTTACAAGTGGTGCCA AGGCATCAATAACCTGCAGGATGTGTGGGAGACGAGTGAGGGTGAGTGCAATGTCATGCTGGAGTCTCGG TTTGAGAAGATGTATGAGAAGATTGACCTGACCCTGCTCAACAGGCTGCTGCGACTCATTGTGGACCACA ACATAGCTGACTACATGACAGCCAAGAACAATGTAGTCATCAACTATAAGGACATGAACCATACAAATTC ATATGGAATCATCAGAGGCCTGCAGTTTGCCTCATTCATTGTACAGTACTATGGTTTGGTGATGGATTTG CTTGTATTGGGATTGCACCGGGCCAGTGAGATGGCTGGGCCTCCGCAGATGCCAAATGACTTTCTCAGTT TTCAGGACATAGCCACCGAGGCTGCACACCCAATCCGACTCTTCTGTAGATACATTGATCGCATCCATAT TTTCTTCAGGTTCACAGCAGATGAGGCTCGGGATCTGATCCAGCGTTACCTGACAGAACATCCAGACCCC AATAATGAAAACATTGTTGGCTACAATAATAAGAAATGCTGGCCCCGAGATGCCCGTATGCGTCTCATGA AGCATGATGTCAACTTGGGTCGGGCAGTGTTCTGGGACATCAAAAACCGGCTGCCACGATCGGTGACTAC AGTACAGTGGGAGAATAGCTTTGTGTCTGTGTACAGTAAAGACAACCCCAACCTGCTGTTCAACATGTGT GGCTTTGAGTGCCGCATCCTACCTAAGTGCCGTACCAGCTATGAAGAGTTCACCCACAAGGACGGGGTCT GGAATCTACAGAATGAGGTTACTAAGGAGCGGACGGCTCAGTGTTTCCTGCGTGTGGACGATGAGTCAAT GCAGCGCTTCCATAACCGCGTGCGGCAGATTCTGATGGCCTCTGGCTCTACCACTTTCACCAAGATTGTG AATAAGTGGAATACAGCTCTCATTGGCCTTATGACATACTTTCGAGAGGCTGTGGTGAATACACAGGAAC TCCTAGACTTACTGGTGAAGTGTGAAAACAAGATCCAGACACGTATCAAGATTGGATTGAACTCCAAGAT GCCAAGTCGGTTCCCCCCTGTTGTATTCTACACCCCTAAGGAGCTGGGTGGGCTTGGCATGCTCTCGATG GGCCATGTCCTTATCCCCCAGTCTGACCTCAGGTGGTCCAAGCAAACAGATGTGGGCATCACACACTTTC GTTCAGGAATGAGTCATGAAGAAGACCAACTGATTCCCAACTTGTACCGCTACATACAGCCATGGGAGAG TGAATTCATTGACTCCCAGCGGGTCTGGGCTGAGTATGCACTCAAGCGACAGGAGGCTATTGCTCAGAAC AGACGCTTGACTTTAGAAGATTTAGAAGACTCGTGGGATCGGGGCATTCCCCGGATCAATACCCTCTTCC AGAAAGATCGGCACACATTGGCTTATGATAAAGGCTGGCGTGTCCGAACTGACTTTAAGCAGTACCAGGT TTTGAAGCAGAACCCCTTTTGGTGGACACATCAACGGCATGATGGCAAGCTTTGGAACCTCAACAACTAC CGCACAGACATGATCCAGGCACTGGGCGGTGTGGAGGGCATTCTGGAACACACTCTCTTCAAGGGCACTT ACTTCCCAACCTGGGAAGGTCTGTTCTGGGAGAAAGCCAGCGGGTTTGAGGAATCCATGAAGTGGAAGAA GCTGACCAATGCTCAGCGATCAGGATTAAACCAGATTCCTAATCGTAGATTTACTCTCTGGTGGTCCCCA ACAATCAATCGAGCCAATGTATACGTAGGCTTCCAGGTGCAGCTGGACCTGACAGGAATCTTTATGCATG GCAAGATCCCCACGCTGAAGATCTCTCTCATCCAGATCTTCCGAGCTCACTTGTGGCAAAAGATTCATGA GAGCATCGTGATGGACTTGTGTCAGGTCTTCGACCAGGAATTAGATGCCCTGGAAATTGAGACAGTACAA AAGGAGACAATCCATCCCCGAAAATCCTACAAGATGAACTCTTCCTGTGCAGATATACTGCTCTTTGCTT CCTATAAGTGGAATGTCTCCCGGCCCTCGCTGCTGGCAGACTCTAAGGATGTGATGGACAGCACCACCAC ACAGAAGTATTGGATTGACATCCAGTTACGCTGGGGGGACTATGATTCCCATGATATTGAGCGCTATGCC CGGGCCAAGTTCCTGGATTACACCACAGACAACATGAGTATCTACCCTTCACCCACAGGTGTGCTCATTG CCATTGATCTGGCCTATAATCTACACAGTGCCTATGGAAACTGGTTCCCGGGGAGCAAGCCTCTCATACA GCAAGCCATGGCCAAGATCATGAAGGCAAACCCTGCCTTGTATGTGTTACGTGAACGGATACGCAAAGGA TTGCAGCTGTATTCATCTGAGCCCACTGAGCCTTACCTGTCCTCTCAGAACTATGGTGAACTCTTCTCGA ACCAGATTATCTGGTTTGTGGATGACACCAATGTCTATAGAGTAACTATCCACAAGACCTTTGAGGGGAA CTTGACAACCAAACCCATCAATGGGGCCATCTTCATCTTCAATCCACGCACAGGACAGCTGTTCCTCAAG ATAATCCACACATCCGTATGGGCTGGACAGAAGCGTTTGGGTCAGTTGGCCAAGTGGAAGACTGCTGAAG AAGTGGCTGCCCTGATCCGATCTCTGCCTGTGGAGGAACAGCCTAAACAGATTATTGTCACTCGGAAAGG GATGCTGGATCCCCTGGAGGTACACTTACTGGACTTCCCCAATATTGTGATCAAAGGATCAGAGCTCCAG CTGCCCTTCCAGGCTTGTCTCAAGGTGGAGAAGTTTGGGGATCTCATCCTTAAAGCCACAGAACCACAGA TGGTTCTCTTCAACCTCTATGATGACTGGCTCAAGACTATCTCATCCTACACGGCTTTCTCCCGTCTCAT CCTGATCCTGCGTGCTTTGCATGTGAACAACGATAGGGCAAAAGTGATCCTGAAGCCAGACAAGACAACC GTTACGGAACCCCACCACATCTGGCCCACGCTCACTGACGAGGAGTGGATCAAGGTGGAGGTGCAGCTCA AGGATCTGATCTTGGCTGACTATGGCAAGAAAAACAATGTGAATGTGGCATCGCTGACACAATCAGAAAT TCGAGACATCATCCTGGGAATGGAGATCTCTGCACCATCGCAGCAGCGGCAGCAGATAGCTGAGATTGAG AAGCAGACCAAGGAACAGTCCCAGCTCACTGCTACACAGACTCGAACTGTCAACAAGCATGGCGATGAGA TCATCACCTCCACCACTAGCAACTATGAGACCCAGACCTTCTCGTCGAAGACCGAATGGAGAGTCAGGGC CATCTCTGCTGCCAACCTGCATCTACGGACCAATCACATTTATGTTTCATCTGATGACATCAAGGAGACT GGTTATACGTACATTCTTCCCAAAAATGTGCTGAAGAAGTTCATCTGCATATCTGATCTGCGCGCCCAAA TTGCAGGATACCTATATGGGGTGAGCCCTCCAGATAACCCTCAGGTGAAGGAGATCCGCTGTATTGTGAT GGTGCCACAGTGGGGCACTCACCAGACTGTGCACCTGCCTAGTCAGCTGCCTCAACATGAGTACCTCAAG GAGATGGAACCCTTAGGTTGGATCCACACTCAACCCAATGAATCCCCCCAGCTATCGCCCCAAGATGTAA CTACTCATGCCAAAATCATGGCTGACAACCCATCTTGGGATGGGGAGAAGACTATTATCATCACCTGCAG CTTCACACCAGGTTCCTGCACACTGACAGCCTACAAGCTGACCCCTAGCGGGTATGAGTGGGGTCGGCAG AACACAGACAAAGGCAACAACCCCAAAGGCTACCTACCTTCGCACTATGAGAGGGTTCAGATGCTCCTGT CTGACCGATTCCTCGGCTTCTTTATGGTCCCTGCCCAGTCCTCCTGGAACTACAACTTTATGGGTGTTCG ACATGACCCCAACATGAAATACGAGCTGCAGCTAGCAAACCCCAAGGAGTTCTACCATGAGGTCCACAGG CCCTCCCACTTCCTCAACTTTGCCCTCCTGCAGGAGGGTGAGGTCTATTCCGCAGACAGAGAGGACCTCT ATGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR217763 representing NM_001191590 Red=Cloning site Green=Tags(s) MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKII RDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVY ISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDH QPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFE PLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAF YFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWV EVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDS HVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFW AAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEG IKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKT VCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYD VEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESR FEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDL LVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDP NNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMC GFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSM GHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQN RRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNY RTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSP TINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQ KETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKG LQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLK IIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQ LPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTT VTEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIE KQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPSQLPQHEYLK EMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQ NTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHR PSHFLNFALLQEGEVYSADREDLYA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001191590
ORF Size	7005 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001191590.1, NP_001178519.1
RefSeq Size	7246 bp
RefSeq ORF	7008 bp
Locus ID	287530
MW	273.6 kDa

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