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NSMCE1 (NM_145080) Human Untagged Clone
CAT＃: SC100078
NSMCE1 (untagged)-Human non-SMC element 1 homolog (S. cerevisiae) (NSMCE1)
CNY 2,400.00
CNY 6,270.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | NSE1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC100078 sequence for NM_145080 edited (data generated by NextGen Sequencing)
ATGCAGGGCAGCACAAGGAGAATGGGCGTCATGACTGATGTCCACCGGCGCTTCCTCCAG TTGCTGATGACCCATGGCGTGCTAGAGGAATGGGACGTGAAGCGCTTGCAGACGCACTGC TACAAGGTCCATGACCGCAATGCCACCGTAGATAAGTTGGAGGACTTCATCAACAACATT AACAGTGTCTTGGAGTCCTTGTATATTGAGATAAAGAGAGGAGTCACGGAAGATGATGGG AGACCCATTTATGCGTTGGTGAATCTTGCTACAACTTCAATTTCCAAAATGGCTACGGAT TTTGCAGAGAATGAACTGGATTTGTTTAGAAAGGCTCTGGAACTGATTATTGACTCAGAA ACCGGCTTTGCGTCTTCCACAAACATATTGAACCTGGTTGATCAACTTAAAGGCAAGAAG ATGAGGAAGAAGGAAGCGGAGCAGGTGCTGCAGAAGTTTGTTCAAAACAAGTGGCTGATT GAGAAGGAAGGGGAGTTCACCCTGCACGGCCGGGCCATCCTGGAGATGGAACAATACATC CGGGAGACGTACCCCGACGCGGTGAAGATCTGCAATATCTGTCACAGCCTCCTCATCCAG GGTCAAAGCTGCGAAACCTGTGGGATCAGGATGCACTTACCCTGCGTGGCCAAGTACTTC CAGTCGAATGCTGAACCGCGCTGCCCCCACTGCAACGACTACTGGCCCCACGAGATCCCA AAAGTCTTCGACCCTGAGAAGGAGAGGGAGTCTGGTGTCTTGAAATCGAACAAAAAGTCC CTGCGGTCCAGGCAGCATTAG Clone variation with respect to NM_145080.3 531 g=>a >OriGene 5' read for NM_145080 unedited
AAGGGTTCACATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCTTGGG CTCCCTGTTCGTTCCCACATGCAGGCAGCACAAGGAGAATGGGCGTCATGACTGATGTCC ACCGGCGCTTCCTCCAGTTGCTGATGACCCATGGCGTGCTAGAGGAATGGGACGTGAAGC GCTTGCAGACGCACTGCTACAAGGTCCATGACCGCAATGCCACCGTAGATAAGTTGGAGG ACTTCATCAACAACATTAACAGTGTCTTGGAGTCCTTGTATATTGAGATAAAGAGAGGAG TCACGGAAGATGATGGGAGACCCATTTATGCGTTGGTGAATCTTGCTACAACTTCAATTT CCAAAATGGCTACGGATTTTGCAGAGAATGAACTGGATTTGTTTAGAAAGGCTCTGGAAC TGATTATTGACTCAGAAACCGGCTTTGCGTCTTCCACAAACATATTGAACCTGGTTGATC AACTTAAAGGCAAGAAGATGAGGAAGAAGGAAGCGGAGCAGGTGCTGCAGAAGTTTGTTC AAAACAAGTGGCTGATTGAGAAGGAAGGGGAGTTCACCCTGCACGGCCGGGCCATCCTGG AGATGGAACAATACATCCGGGAGACGTACCCCGACGCGGTGAAGATCTGCAATATCTGTC ACAGCCTCCTCATCCAGGGTCAAAGCTGCGAAACCTGTGGGATCANGATGCACTTACCCT GCGTGGCCAAGTACTTTCAGTCGAATGCTGAACCGCGCTGCCCCCACTGCAACGACTACT GGCCCCACGAGATCCCAAAAGTCTTCGACCCTGAGAAAGAGAGGGAGTCTGGNTGTCTTG AAATCGAACAAAAAGTCCCTGNNCGTCCAGCAGCATTAGCCATCGTGCCCTGT >OriGene 3' read for NM_145080 unedited
ATGGACCGGCGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTGCAGCAACTTCCCAGGATGGGTTATTCCAAAGCTGTGGACGGGGA ACATTAAAACCAAAGAGGGGACTCGCGTGGAACCTGAAACACAAACCCCTTTTTTCCAAA AAGGGCTGTGGCGATCAGGCCACTCAAGGGAGCCAGCCCCTCAACAGGGCACGATGGCTA ATGCTGCCTGGACCGCAAGGACTTTTTGTTCGATTTCAAGACACCAGACTCCCTCTCCTT TTCAGGGTCGAAAACTTTTGGGATCTCGTGGGGCCCGTAATCCTTGCAGTGGGGGCAACG CGGTTCAACATTCCACTGGAAATACTTGGCCACCCCGGGGAAGTGCATCCTGATCCCCAG GTTTCGCAACTTTGACCCTGGATGAAGAGGCTGTGACAAATATTGCAGATCTTCACCGCG TCGGGGTACCTCTCCCGGATGTATTGGTCCATCTCCACGATGGCCCCGCCCTGCAAGGTG AACTCCCCTTTCTTCTCAATCAGCCACTTGTTTTGAACAAACTTCTGGAACACCTGCTCC GCTTCCTTCTTCCTCAACTTCTTGGCCTTAAGTTGATCAACCCGGTTCAATATGTTTGTG GAAGACGCAAACCCGGTTCTGAGTCAATATCAATTTCCGACCCTTCTAAACAATCCCATT CATTCTCTGGAAAACCCGTACCTTTTTGGAAATTGAGTTGTAGCAAGATTCACCAACGCA TAATGGGTCTCCCCATAACTTTCCGGACTTCCCTCTTATATTCAAATACAAGACTTCAAA AACTGTTAAGGTGGTGAGGAAACCCTCAACTATCTCCGGGGCTTGCGGGCATGGACCTGT TAAATGCTTTTGAACCCTTAATTTCATCCTTTAGACGCCCTGGTATCACAACTGAGAAAG CC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_145080 |
| Insert Size | 1000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145080.2, NP_659547.1 |
| RefSeq Size | 983 bp |
| RefSeq ORF | 771 bp |
| Locus ID | 197370 |
| UniProt ID | Q8WV22 |
| Protein Families | Druggable Genome |
| Gene Summary | RING-type zinc finger-containing E3 ubiquitin ligase that assembles with melanoma antigen protein (MAGE) to catalyze the direct transfer of ubiquitin from E2 ubiquitin-conjugating enzyme to a specific substrate. Within MAGE-RING ubiquitin ligase complex, MAGE stimulates and specifies ubiquitin ligase activity likely through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. Involved in maintenance of genome integrity, DNA damage response and DNA repair (PubMed:29225034, PubMed:20864041). NSMCE3/MAGEG1 and NSMCE1 ubiquitin ligase are components of SMC5-SMC6 complex and may positively regulate homologous recombination-mediated DNA repair (PubMed:18086888). MAGEF1-NSMCE1 ubiquitin ligase promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors (PubMed:29225034).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218073 | NSMCE1 (Myc-DDK-tagged)-Human non-SMC element 1 homolog (S. cerevisiae) (NSMCE1) |
CNY 2,400.00 |
|
| RC218073L3 | Lenti ORF clone of Human non-SMC element 1 homolog (S. cerevisiae) (NSMCE1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218073L4 | Lenti ORF clone of Human non-SMC element 1 homolog (S. cerevisiae) (NSMCE1), mGFP tagged |
CNY 5,890.00 |
|
| RG218073 | NSMCE1 (tGFP-tagged) - Human non-SMC element 1 homolog (S. cerevisiae) (NSMCE1) |
CNY 4,000.00 |
|
| SC317383 | NSMCE1 (untagged)-Human non-SMC element 1 homolog (S. cerevisiae) (NSMCE1) |
CNY 6,270.00 |
