LIPT1 (NM_145197) Human Untagged Clone
CAT#: SC100187
LIPT1 (untagged)-Human lipoyltransferase 1 (LIPT1), nuclear gene encoding mitochondrial protein, transcript variant 3
CNY 6,080.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | LIPT1D |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_145197, the custom clone sequence may differ by one or more nucleotides
ATGCTGATCCCATTTTCAATGAAGAATTGCTTCCAGTTACTTTGTAACTGCCAGGTCCCAGCAGCTGGCT TTAAAAAAACAGTAAAAAATGGGCTCATTTTACAGTCAATTTCCAATGATGTCTATCAAAATCTGGCTGT GGAAGACTGGATCCATGACCATATGAATCTAGAAGGCAAACCAATTCTATTCTTTTGGCAGAATTCTCCC TCTGTTGTAATTGGTAGGCATCAAAATCCTTGGCAGGAATGTAACCTGAATCTAATGAGAGAAGAAGGTA TAAAACTGGCTCGGAGAAGAAGTGGAGGAGGAACAGTCTACCATGATATGGGTAATATCAATTTGACTTT CTTTACAACCAAAAAAAAGTATGATAGAATGGAAAATCTGAAATTAATTGTGAGAGCTCTGAATGCTGTC CAACCCCAGCTGGATGTGCAGGCTACCAAAAGATTTGACCTTTTACTTGATGGACAGTTTAAAATCTCAG GAACAGCTTCTAAGATCGGCCGGACTACTGCCTATCACCATTGCACTTTATTATGTAGTACTGATGGGAC GTTCTTGTCTTCTTTGCTAAAGAGCCCTTACCAAGGGATCAGGAGCAATGCCACTGCTAGCATACCTTCC TTAGTGAAAAATCTTTTGGAAAAGGATCCCACTCTGACCTGTGAAGTACTAATGAATGCTGTTGCTACAG AGTATGCTGCTTATCATCAAATTGATAATCACATTCACCTAATAAACCCAACGGATGAGACACTGTTTCC TGGAATAAATAGCAAAGCCAAAGAACTGCAAACTTGGGAGTGGATATATGGCAAAACTCCAAAGTTTAGT ATAAATACTTCCTTTCATGTGTTATATGAACAGTCACACTTGGAAATTAAAGTATTCATAGACATAAAGA ATGGAAGAATTGAAATTTGTAATATTGAAGCACCTGATCATTGGTTGCCATTGGAAATACGTGACAAATT AAATTCAAGTCTTATTGGCAGTAAGTTTTGCCCAACTGAAACTACCATGCTAACAAATATATTACTTAGA ACATGTCCACAAGACCACAAACTAAACAGTAAATGGAATATTCTCTGTGAAAAAATTAAGGGAATAATGT GA >OriGene 5' read for NM_145197 unedited
NGGGGTTCACATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGNAGCACT TTGGGAGGCCAAGTGGGAGGACTGCTTGAGCCCAGGAGTTTGAGACCAGCCTGGGCAACA TGGTGAAATCCTGTCTGTAAGAGAAAAAAAAAAGATATATTACATAATGTATTTGCAGCA GACTTATTGTTTGAAAACTGTGAAATTATTTCAACTGAAATTATTTGAAATTTGTCTTCA TTTATTTCTTTTGAATACTTGGTTATAGCATTGCTAAAGACTAATTGTAAAGCTAAAAGC CAAAAGCAACTGCACTGGATACTTTAAGTTTGGATTAAATAACCGATAATTGATAGTTAG AAAATAGAATTTAATGTTCCTTTTCTCGATGAATTAATTTGTTTGTCTTCCATTTTTAAA AGCATGCTGATCCCATTTTCAATGAAGAATTGCTTCCAGTTACTTTGTAACTGCCAGGTC CCAGCAGCTGGCTTTAAAAAAACAGTAAAAAATGGGCTCATTTTACAGTCAATTTCCAAT GATGTCTATCAAAATCTGGCTGTGGAAGACTGGATCCATGACCATATGAATCTAGAAGGC AAACCAATTCTATTCTTTTGGCAGAATTCTCCCTCTGTTGTAATTGGTAGGCATCAAAAT CCTTGGCAGGAATGTAACCTGNATCTAATGAGAGAAAGAAGTATAAAACTGGCTCGGAGA AGAAGTGGNAGAGGAACAGTCTACCATGATATGGGTAATATCAATTTGACTTTCTTTACA CCCAAAAAAAGTTGATAGAATGGGAAAATCTGAATTAATTGTGAGAGCTCTGAATGCTGT CCAACCCCAGCTGGATGTGCAAGCTACCAAAAGATTTGACCTTTTACTTGATGGAACAGT TTAAATCTCAG |
Restriction Sites | NotI-NotI |
ACCN | NM_145197 |
Insert Size | 3000 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_145197.1, NP_660198.1 |
RefSeq Size | 1543 bp |
RefSeq ORF | 1122 bp |
Locus ID | 51601 |
UniProt ID | Q9Y234 |
Domains | BPL_LipA_LipB |
Protein Pathways | Lipoic acid metabolism, Metabolic pathways |
Gene Summary | The process of transferring lipoic acid to proteins is a two-step process. The first step is the activation of lipoic acid by lipoate-activating enzyme to form lipoyl-AMP. For the second step, the protein encoded by this gene transfers the lipoyl moiety to apoproteins. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 13. Read-through transcription also exists between this gene and the neighboring downstream mitochondrial ribosomal protein L30 (MRPL30) gene. [provided by RefSeq, Mar 2011] Transcript Variant: This variant (3), also known as transcript C, contains an additional exon in the 5' UTR, compared to variant 1. Variants 1 and 3-6 all encode the same protein. There are no publicly available transcripts representing this variant; it is supported by data in PMID:10103005. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218958 | LIPT1 (Myc-DDK-tagged)-Human lipoyltransferase 1 (LIPT1), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 3,656.00 |
|
RC218958L3 | Lenti ORF clone of Human lipoyltransferase 1 (LIPT1), nuclear gene encoding mitochondrial protein, transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC218958L4 | Lenti ORF clone of Human lipoyltransferase 1 (LIPT1), nuclear gene encoding mitochondrial protein, transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
RG218958 | LIPT1 (tGFP-tagged) - Human lipoyltransferase 1 (LIPT1), nuclear gene encoding mitochondrial protein, transcript variant 3 |
CNY 5,256.00 |