Apolipoprotein L 1 (APOL1) (NM_145344) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APO-L; APOL; APOL, APO-L, APOL-I; APOL-I; apolipoprotein L, 1; apolipoprotein L-I; apolipoprotein L1; OTTHUMP00000028705
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC100197 sequence for NM_145344 edited (data generated by NextGen Sequencing) ATGGAGGGAGCTGCTTTGCTGAGAGTCTCTGTCCTCTGCATCTGGATGAGTGCACTTTTC CTTGGTGTGGGAGTGAGGGCAGAGGAAGCTGGAGCGAGGGTGCAACAAAACGTTCCAAGT GGGACAGATACTGGAGATCCTCAAAGTAAGCCCCTCGGTGACTGGGCTGCTGGCACCATG GACCCAGAGAGCAGTATCTTTATTGAGGATGCCATTAAGTATTTCAAGGAAAAAGTGAGC ACACAGAATCTGCTACTCCTGCTGACTGATAATGAGGCCTGGAACGGATTCGTGGCTGCT GCTGAACTGCCCAGGAATGAGGCAGATGAGCTCCGTAAAGCTCTGGACAACCTTGCAAGA CAAATGATCATGAAAGACAAAAACTGGCACGATAAAGGCCAGCAGTACAGAAACTGGTTT CTGAAAGAGTTTCCTCGGTTGAAAAGTAAGCTTGAGGATAACATAAGAAGGCTCCGTGCC CTTGCAGATGGGGTTCAGAAGGTCCACAAAGGCACCACCATCGCCAATGTGGTGTCTGGC TCTCTCAGCATTTCCTCTGGCATCCTGACCCTCGTCGGCATGGGTCTGGCACCCTTCACA GAGGGAGGCAGCCTTGTACTCTTGGAACCTGGGATGGAGTTGGGAATCACAGCAGCTTTG ACCGGGATTACCAGCAGTACCATAGACTACGGAAAGAAGTGGTGGACACAAGCCCAAGCC CACGACCTGGTCATCAAAAGCCTTGACAAATTGAAGGAGGTGAAGGAGTTTTTGGGTGAG AACATATCCAACTTTCTTTCCTTAGCTGGCAATACTTACCAACTCACACGAGGCATTGGG AAGGACATCCGTGCCCTCAGACGAGCCAGAGCCAATCTTCAGTCAGTACCGCATGCCTCA GCCTCACGCCCCCGGGTCACTGAGCCAATCTCAGCTGAAAGCGGTGAACAGGTGGAGAGG GTTAATGAACCCAGCATCCTGGAAATGAGCAGAGGAGTCAAGCTCACGGATGTGGCCCCT GTAAGCTTCTTTCTTGTGCTGGATGTAGTCTACCTCGTGTACGAATCAAAGCACTTACAT GAGGGGGCAAAGTCAGAGACAGCTGAGGAGCTGAAGAAGGTGGCTCAGGAGCTGGAGGAG AAGCTAAACATTCTCAACAATAATTATAAGATTCTGCAGGCGGACCAAGAACTGTGA Clone variation with respect to NM_145344.1 448 g=>a;654 c=>a;684 g=>a;764 g=>a 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_145344 unedited AATTTGTACTACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTGGAGGTGGGA TCCACACAGCTCAGACAGCTGGATCTTGCTCAGTCTCTGCCAGGGGAAGATTCCTTGGAG GAGGCCCTGCAGCGACATGGAGGGAGCTGCTTTGCTGAGAGTCTCTGTCCTCTGCATCTG GATGAGTGCACTTTTCCTTGGTGTGGGAGTGAGGGCAGAGGAAGCTGGAGCGAGGGTGCA ACAAAACGTTCCAAGTGGGACAGATACTGGAGATCCTCAAAGTAAGCCCCTCGGTGACTG GGCTGCTGGCACCATGGACCCAGAGAGCAGCATCTTTATTGAGGATGCCATTAAGTATTT CAAGGAAAAAGTGAGCACACAGAATCTGCTACTCCTGCTGACTGATAATGAGGCCTGGAA CGGATTCGTGGCTGCTGCTGAACTGCCCAGGAATGAGGCAGATGAGCTCCGTAAAGCTCT GGACAACCTTGCAAGACAAATGATCATGACAGACAAAAACTGGCACGATAAAGGCCAGCA GTACAGAAACTGGTTTCTGAAAGAGTTTCCTCGGTTGAAAAGTAAGCTTGAGGATAACAT AAGAAGGCTCCGTGCCCTTGCAGATGGGGTTCAGAAGGCCCACAAAGGCACCCACCATCG CCAATGTGGTGTCTGGCTCTCTCAACATTTCCTCTGGCATCCTGACCCTCGTCGGCATGG GTCTGGCACCCTTCACAGAGGGAGGCACCCCTGCACTCTTGGACCTGNGATGGAGTTGGG AATCACAACCGCTCTCGACGGGATTACCCCCCGTACCATAGCCTCACCGAAGGAAGTGGG GACAACAAGCCCCAGCCCACGACCTGGCCATAAAAGCCTTGACAATTGN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_145344 unedited TAGCTCTGNAACGCCGCACGCATCTAGNGATCGAGTTTTTTTTTTTTTTTTTTGATGAGT AGGTGAGTTTATTGGGACTTACACACAGGTCAATCCTGGGCGGCGACAAGACAGCTCTAG AGATCTGAGCTTCCTCCCAATGCTAAACTGCTTTCATGCTAATTTTCTGACTGTTTACTT ACCGGGTAAGAGCGATGGGACTGTTTTCATTGGTTGGTTCTCACATACTCTCTGGGAAGT TTGGGTTCTCAGGGACACCTGCTCCTCAGCTGGGGACCATGGCCATGGCCCACCACCTGC CCTTCAGTGTTCAAGCAGGGGACATGCACCCTTTAGTAACCTGGAGGGGACCCATCACAT GACAACCACCCCAACGACCATCATCAGGAAGCCGCTGCCTGACTGAGATATGCCCCCAGG AGGACAAGGGAGAGTGGATGCTGGAAAGACAGGGCAGGGGACCATCACCAGGGAAAGACT TCATTCTTCGGAGGACATTGAACCTGGGGCTGGGTCTGTAGTGGAGCCGCTGTTTCTTCT CCTGTATCCAACTGTTCTAACTCTTGGGCTTTCTCCATTTTCAGCTCTTTCTTTTCCTGG CCTTCTCATTGCTGGNTCCTTCAAGCCTCCTCTCTATTCTTCCGTCAATATATCTCTTTT CCTTTTTTTCCCTGAGATGGAGTCTCGCTCTGTCACCCAGCCTGGAGTGCAGTGGCGACA TCTCGGCTCACTGCAAGCTCCAACCTGGCGACAGAGCAAGACTCTGTCTCAGAATAAAAA AAAGCTTGCATTTTGTCCTGGCCCCTGCCAAGCATATCTCTCCTGGTGGCTGCCCTGCCC TGTGGTCACAGTCTTGGTCCGCCTGCAGAATTTATAATTATTGGTGAGGAAGGTTAACTT CTTCTTCAGCTCCTGAGCCACCCTTTTCAACTCCTCAGCTGGCTTTGACTCTGCCCCTCC AGTGAGAGCTTTN
Restriction Sites	NotI-NotI
ACCN	NM_145344
Insert Size	2150 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_145344.1, NP_663319.1
RefSeq Size	2158 bp
RefSeq ORF	1197 bp
Locus ID	8542
Protein Families	Secreted Protein, Transmembrane
Gene Summary	This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008] Transcript Variant: This variant (3) differs in the 3' UTR compared to variant 1. Both variants 1 and 3 encode isoform a.

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