RBJ (DNAJC27) (NM_016544) Human Untagged Clone

CAT#: SC100369

DNAJC27 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1



  "NM_016544" in other vectors (6)

CNY 3,600.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DNAJC27 mouse monoclonal antibody,clone OTI7A4
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms RabJS; RBJ
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC100369 sequence for NM_016544 edited (data generated by NextGen Sequencing)
ATGGAGGCCAACATGCCGAAGCGGAAGGAGCCTGGCAGGTCTCTCCGCATCAAAGTCATC
TCCATGGGCAACGCCGAAGTGGGGAAAAGCTGTATTATAAAGCGATACTGTGAGAAAAGA
TTCGTGTCTAAATACCTGGCAACAATTGGAATTGACTATGGAGTCACAAAGGTACACGTC
AGAGACAGAGAAATCAAAGTTAACATCTTTGATATGGCTGGACATCCCTTCTTCTATGAG
GTTCGAAATGAGTTTTACAAGGACACACAGGGTGTGATACTGGTCTATGATGTTGGGCAG
AAAGACTCCTTTGACGCCCTTGATGCGTGGCTGGCAGAAATGAAGCAAGAGCTTGGACCT
CATGGAAACATGGAAAATATTATATTTGTAGTTTGTGCCAACAAGATTGATTGTACCAAA
CATCGCTGTGTAGATGAAAGTGAAGGACGTCTTTGGGCTGAAAGCAAAGGGTTCCTGTAC
TTTGAAACTTCAGCACAAACTGGAGAAGGCATTAATGAGATGTTCCAGACCTTTTATATA
TCCATAGTTGATTTATGTGAAAATGGCGGGAAACGCCCTACCACCAATAGCAGTGCTAGT
TTCACCAAAGAACAAGCAGATGCCATTCGCAGAATTCGAAATAGTAAAGACAGTTGGGAC
ATGCTGGGAGTCAAACCTGGGGCCTCAAGGGATGAAGTCAATAAAGCGTATCGGAAACTT
GCTGTGCTTCTTCACCCTGACAAATGTGTAGCACCTGGCAGTGAAGATGCCTTCAAAGCA
GTTGTGAATGCTCGGACAGCCCTCCTGAAAAACATCAAGTAG

Clone variation with respect to NM_016544.2
33 c=>t
>OriGene 5' read for NM_016544 unedited
CTCTTGTATACGACTCCTATAGGCGGCCGCGAAATTCGCACGAGGGAAAAGACCGAGGCG
GGTGGNCCCCGNATAGAGCCAGGGCCATGGAGGCCAACATGCCGAAGCGGAAGGAGCCTG
GCAGGTCTCTCCGCATCAAAGTCATCTCCATGGGCAACGCCNAAGTGGGGAAAAGCTGTA
TTATAAAGCGATACTGTGAGAAAAGATTCGTGTCTAAATACCTGGCAACAATTGGAATTG
ACTATGGAGTCACAAAGGTACACGTCAGAAACAGAGAAATCAAAGTTAACATCTTTGATA
TGGCTGGACATCCCTTCTTCTATGAGGTTCGAAATGAGTTTTACAAGGACACACAGGGTG
TGATACTGGTCTATGATGTTGGGCAGAAAGACTCCTTTGACGCCCTTGATGCGTGGCTGG
CAGAAATGAAGCAAGAGCTTGGACCTCATGGAAACATGGAAAATATTATATTTGTAGTTT
GTGCCAACAAGATTGATTGTACCAAACATCGCTGTGTAGATGAAAGTGAAGGACGTCTTT
GGGCTGAAGCAAAGGGTTCCTGTACTTTGAAACTTCAGCACAAACTGGAGAAGGCATTAA
TGAGATGTTCCAGAACTTTTATATATCCATAGTTGATTTATGTGAAAATGGCGGGAAACG
CCCTACCCCAATAGCAGTGCTAGTTTCACCAAAAACAAGCAGATGCCATTCGCAGAATTC
GAAATAGTANAGACAGTTGGGACATGCCTTGGAAGTCAAACCTGGGCCTCAAGGAATATG
AGTCATTNAAGCGTATCGAAACTTTTGCTGTGCTTCTTCACCTGACAAATGTGTAGACCT
TTTGCAANGGAAAATGCTTCAAAAAAAAATTTGGGATTTGCTCGAGACAGNCCTTCTTGA
AACATAGGTTGANAA
>OriGene 3' read for NM_016544 unedited
AGCTTGTACGCGGCCGCTTCTANGATCGAGTTTTTTTTTTTTTTTTTTAAAAAACTGATT
CAGAGCTTGCAGTGGCTGCTTACTTACTCTGGCTATCTTTCAGAAATTTAGCTGCCTTAT
CTATAGCTCATTTAACTAACAGTGAATTAGGTACTAGTTTATAGGTATAAAGAAATAGTT
ACTTAGTTGAGAATTCAATATATGAGAACATACTAGAGTAAGTTTCCTGAGGTAAAAGTA
TGCCTTAAAGAATGATGTGCAATCTATAAAACTGTAGCATTTTCTCTTGGGTAGCAACAC
AATGACCCCCAAGTCTGGATTTTTAAATTAACAGGCTAAGAAAACTAAGGACACATTGAT
ATAGAGAAATAAAATAACAATGCTGAAGTCAAAGAAATATTGACAGCTGAGATATCAATT
CAAAAGGAGGCATGAAGGGGAACAGAGGAACAGAAGGAGGCTGTATTTTAAAGTGCTTAG
GTAGGTTAGAAGATGAATTTGAAATGCCTCCAGGGATTTCTAACAAGTTTAAAAAGTGTT
ACTTCTTGGTGACTCAGATGTTGAAGACATGGCTTTTCTTTATAATAGCTATTCAAACTT
TTTATTCTTTTTTGAAAAACAAGATTCTCTATAAAAACGTTTTAGCCATTGGTATTATTC
TGCTAGCTGTTACTCTCACTAATGTATTTTGAAATCCCTACTGGCTGAAGAATTTTTAAT
AAGGTTGAACTACAAAGCACACATTTGCACCAAGAATTTTAATTAATGCATAGATGTCTG
TGAAAATCTGCATGAAGTTATGGCAAAAAAACCTTTTCCATGGAGATCATGCTTTAATTC
CTAATATTTCTGCTGGAGGGATATTTGAAATCCGAAAAATTCTTGGGACCCTGGAGCTTA
ACTTGGCTAATTTTATTCCCTTTTACGTAAATGAAGTGGTATATGGCGCATTCCTTACAT
TAA
Restriction Sites NotI-NotI     
ACCN NM_016544
Insert Size 4700 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_016544.1, NP_057628.1
RefSeq Size 4869 bp
RefSeq ORF 822 bp
Locus ID 51277
UniProt ID Q9NZQ0
Domains DnaJ, ras, RAN, RAS, RHO, RAB
Gene Summary GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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