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RBJ (DNAJC27) (NM_016544) Human Untagged Clone
CAT＃: SC100369
DNAJC27 (untagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1
CNY 3,600.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | RabJS; RBJ |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC100369 sequence for NM_016544 edited (data generated by NextGen Sequencing)
ATGGAGGCCAACATGCCGAAGCGGAAGGAGCCTGGCAGGTCTCTCCGCATCAAAGTCATC TCCATGGGCAACGCCGAAGTGGGGAAAAGCTGTATTATAAAGCGATACTGTGAGAAAAGA TTCGTGTCTAAATACCTGGCAACAATTGGAATTGACTATGGAGTCACAAAGGTACACGTC AGAGACAGAGAAATCAAAGTTAACATCTTTGATATGGCTGGACATCCCTTCTTCTATGAG GTTCGAAATGAGTTTTACAAGGACACACAGGGTGTGATACTGGTCTATGATGTTGGGCAG AAAGACTCCTTTGACGCCCTTGATGCGTGGCTGGCAGAAATGAAGCAAGAGCTTGGACCT CATGGAAACATGGAAAATATTATATTTGTAGTTTGTGCCAACAAGATTGATTGTACCAAA CATCGCTGTGTAGATGAAAGTGAAGGACGTCTTTGGGCTGAAAGCAAAGGGTTCCTGTAC TTTGAAACTTCAGCACAAACTGGAGAAGGCATTAATGAGATGTTCCAGACCTTTTATATA TCCATAGTTGATTTATGTGAAAATGGCGGGAAACGCCCTACCACCAATAGCAGTGCTAGT TTCACCAAAGAACAAGCAGATGCCATTCGCAGAATTCGAAATAGTAAAGACAGTTGGGAC ATGCTGGGAGTCAAACCTGGGGCCTCAAGGGATGAAGTCAATAAAGCGTATCGGAAACTT GCTGTGCTTCTTCACCCTGACAAATGTGTAGCACCTGGCAGTGAAGATGCCTTCAAAGCA GTTGTGAATGCTCGGACAGCCCTCCTGAAAAACATCAAGTAG Clone variation with respect to NM_016544.2 33 c=>t >OriGene 5' read for NM_016544 unedited
CTCTTGTATACGACTCCTATAGGCGGCCGCGAAATTCGCACGAGGGAAAAGACCGAGGCG GGTGGNCCCCGNATAGAGCCAGGGCCATGGAGGCCAACATGCCGAAGCGGAAGGAGCCTG GCAGGTCTCTCCGCATCAAAGTCATCTCCATGGGCAACGCCNAAGTGGGGAAAAGCTGTA TTATAAAGCGATACTGTGAGAAAAGATTCGTGTCTAAATACCTGGCAACAATTGGAATTG ACTATGGAGTCACAAAGGTACACGTCAGAAACAGAGAAATCAAAGTTAACATCTTTGATA TGGCTGGACATCCCTTCTTCTATGAGGTTCGAAATGAGTTTTACAAGGACACACAGGGTG TGATACTGGTCTATGATGTTGGGCAGAAAGACTCCTTTGACGCCCTTGATGCGTGGCTGG CAGAAATGAAGCAAGAGCTTGGACCTCATGGAAACATGGAAAATATTATATTTGTAGTTT GTGCCAACAAGATTGATTGTACCAAACATCGCTGTGTAGATGAAAGTGAAGGACGTCTTT GGGCTGAAGCAAAGGGTTCCTGTACTTTGAAACTTCAGCACAAACTGGAGAAGGCATTAA TGAGATGTTCCAGAACTTTTATATATCCATAGTTGATTTATGTGAAAATGGCGGGAAACG CCCTACCCCAATAGCAGTGCTAGTTTCACCAAAAACAAGCAGATGCCATTCGCAGAATTC GAAATAGTANAGACAGTTGGGACATGCCTTGGAAGTCAAACCTGGGCCTCAAGGAATATG AGTCATTNAAGCGTATCGAAACTTTTGCTGTGCTTCTTCACCTGACAAATGTGTAGACCT TTTGCAANGGAAAATGCTTCAAAAAAAAATTTGGGATTTGCTCGAGACAGNCCTTCTTGA AACATAGGTTGANAA >OriGene 3' read for NM_016544 unedited
AGCTTGTACGCGGCCGCTTCTANGATCGAGTTTTTTTTTTTTTTTTTTAAAAAACTGATT CAGAGCTTGCAGTGGCTGCTTACTTACTCTGGCTATCTTTCAGAAATTTAGCTGCCTTAT CTATAGCTCATTTAACTAACAGTGAATTAGGTACTAGTTTATAGGTATAAAGAAATAGTT ACTTAGTTGAGAATTCAATATATGAGAACATACTAGAGTAAGTTTCCTGAGGTAAAAGTA TGCCTTAAAGAATGATGTGCAATCTATAAAACTGTAGCATTTTCTCTTGGGTAGCAACAC AATGACCCCCAAGTCTGGATTTTTAAATTAACAGGCTAAGAAAACTAAGGACACATTGAT ATAGAGAAATAAAATAACAATGCTGAAGTCAAAGAAATATTGACAGCTGAGATATCAATT CAAAAGGAGGCATGAAGGGGAACAGAGGAACAGAAGGAGGCTGTATTTTAAAGTGCTTAG GTAGGTTAGAAGATGAATTTGAAATGCCTCCAGGGATTTCTAACAAGTTTAAAAAGTGTT ACTTCTTGGTGACTCAGATGTTGAAGACATGGCTTTTCTTTATAATAGCTATTCAAACTT TTTATTCTTTTTTGAAAAACAAGATTCTCTATAAAAACGTTTTAGCCATTGGTATTATTC TGCTAGCTGTTACTCTCACTAATGTATTTTGAAATCCCTACTGGCTGAAGAATTTTTAAT AAGGTTGAACTACAAAGCACACATTTGCACCAAGAATTTTAATTAATGCATAGATGTCTG TGAAAATCTGCATGAAGTTATGGCAAAAAAACCTTTTCCATGGAGATCATGCTTTAATTC CTAATATTTCTGCTGGAGGGATATTTGAAATCCGAAAAATTCTTGGGACCCTGGAGCTTA ACTTGGCTAATTTTATTCCCTTTTACGTAAATGAAGTGGTATATGGCGCATTCCTTACAT TAA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_016544 |
| Insert Size | 4700 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016544.1, NP_057628.1 |
| RefSeq Size | 4869 bp |
| RefSeq ORF | 822 bp |
| Locus ID | 51277 |
| UniProt ID | Q9NZQ0 |
| Domains | DnaJ, ras, RAN, RAS, RHO, RAB |
| Gene Summary | GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205500 | DNAJC27 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1 |
CNY 3,600.00 |
|
| RC205500L1 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC205500L2 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC205500L3 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC205500L4 | Lenti ORF clone of Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG205500 | DNAJC27 (tGFP-tagged) - Human DnaJ (Hsp40) homolog, subfamily C, member 27 (DNAJC27), transcript variant 1 |
CNY 5,200.00 |
