SP1 (NM_138473) Human Untagged Clone

Name: SP1 (NM_138473) Human Untagged Clone
SKU: SC101137
Price: 8632.00

CAT＃: SC101137

SP1 (untagged)-Human Sp1 transcription factor (SP1), transcript variant 1

Datasheet

SDS

"NM_138473" in other vectors (6)

CNY 8,632.00

货期*

现货

规格

Cited in 20 publications.

Product images

经常一起买 (3)

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CNY 4,090.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA322073

Anti-SP1 Rabbit Polyclonal Antibody

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_138473 edited TCGGCACGAGGCTTGCCTCGTCAGCGTCCGCGTTTTTCCCGGCCCCCCCCAACCCCCCCG GACAGGACCCCCTTGAGCTTGTCCCTCAGCTGCCACCATGAGCGACCAAGATCACTCCAT GGATGAAATGACAGCTGTGGTGAAAATTGAAAAAGGAGTTGGTGGCAATAATGGGGGCAA TGGTAATGGTGGTGGTGCCTTTTCACAGGCTCGAAGTAGCAGCACAGGCAGTAGCAGCAG CACTGGAGGAGGAGGGCAGGAGTCCCAGCCATCCCCTTTGGCTCTGCTGGCAGCAACTTG CAGCAGAATTGAGTCACCCAATGAGAACAGCAACAACTCCCAGGGCCCGAGTCAGTCAGG GGGAACAGGTGAGCTTGACCTCACAGCCACACAACTTTCACAGGGTGCCAATGGCTGGCA GATCATCTCTTCCTCCTCTGGGGCTACCCCTACCTCAAAGGAACAGAGTGGCAGCAGTAC CAATGGCAGCAATGGCAGTGAGTCTTCCAAGAATCGCACAGTCTCTGGTGGGCAGTATGT TGTGGCTGCCGCTCCCAACTTACAGAACCAGCAAGTTCTGACAGGACTACCTGGAGTGAT GCCTAATATTCAGTATCAAGTAATCCCACAGTTCCAGACCGTTGATGGGCAACAGCTGCA GTTTGCTGCCACTGGGGCCCAAGTGCAGCAGGATGGTTCTGGTCAAATACAGATCATACC AGGTGCAAACCAACAGATTATCACAAATCGAGGAAGTGGAGGCAACATCATTGCTGCTAT GCCAAACCTACTCCAGCAGGCTGTCCCCCTCCAAGGCCTGGCTAATAATGTACTCTCAGG ACAGACTCAGTATGTGACCAATGTACCAGTGGCCCTGAATGGGAACATCACCTTGCTACC TGTCAACAGCGTTTCTGCAGCTACCTTGACTCCCAGCTCTCAGGCAGTCACGATCAGCAG CTCTGGGTCCCAGGAGAGTGGCTCACAGCCTGTCACCTCAGGGACTACCATCAGTTCTGC CAGCTTGGTATCATCACAAGCCAGTTCCAGCTCCTTTTTCACCAATGCCAATAGCTACTC AACTACTACTACCACCAGCAACATGGGAATTATGAACTTTACTACCAGTGGATCATCAGG GACCAACTCTCAAGGCCAGACACCCCAGAGGGTCAGTGGGCTACAGGGGTCTGATGCTCT GAACATCCAGCAAAACCAGACATCTGGAGGCTCATTGCAAGCAGGCCAGCAAAAAGAAGG AGAGCAAAACCAGCAGACACAGCAGCAACAAATTCTTATCCAGCCTCAGCTAGTTCAAGG GGGACAGGCCCTCCAGGCCCTCCAAGCAGCACCATTGTCAGGGCAGACCTTTACAACTCA AGCCATCTCCCAGGAAACCCTCCAGAACCTCCAGCTTCAGGCTGTTCCAAACTCTGGTCC CATCATCATCCGGACACCAACAGTGGGGCCCAATGGACAGGTCAGTTGGCAGACTCTACA GCTGCAGAACCTCCAAGTTCAGAACCCACAAGCCCAAACAATCACCTTAGCCCCAATGCA GGGTGTTTCCTTGGGGCAGACCAGCAGCAGCAACACCACTCTCACACCCATTGCCTCAGC TGCTTCCATTCCTGCTGGCACAGTCACTGTGAATGCTGCTCAACTCTCCTCCATGCCAGG CCTCCAGACCATTAACCTCAGTGCATTGGGTACTTCAGGAATCCAGGTGCACCCAATTCA AGGCCTGCCGTTGGCTATAGCAAATGCCCCAGGTGATCATGGAGCTCAGCTTGGTCTCCA TGGGGCTGGTGGTGATGGAATACATGATGACACAGCAGGTGGAGAGGAAGGAGAAAACAG CCCAGATGCCCAACCCCAAGCCGGTCGGAGGACCCGGCGGGAAGCATGCACCTGCCCCTA CTGTAAAGACAGTGAAGGAAGGGGCTCGGGGGATCCTGGCAAAAAGAAACAGCATATTTG CCACATCCAAGGCTGTGGGAAAGTGTATGGCAAGACCTCTCACCTGCGGGCACACTTGCG CTGGCATACAGGCGAGAGGCCATTTATGTGTACCTGGTCATACTGTGGGAAACGCTTCAC ACGTTCGGATGAGCTACAGAGGCACAAACGTACACACACAGGTGAGAAGAAATTTGCCTG CCCTGAGTGTCCTAAGCGCTTCATGAGGAGTGACCACCTGTCAAAACATATCAAGACCCA CCAGAATAAGAAGGGAGGCCCAGGTGTAGCTCTGAGTGTGGGCACTTTGCCCCTGGACAG TGGGGCAGGTTCAGAAGGCAGTGGCACTGCCACTCCTTCAGCCCTTATTACCACCAATAT GGTAGCCATGGAGGCCATCTGTCCAGAGGGCATTGCCCGTCTTGCCAACAGTGGCATCAA CGTCATGCAGGTGGCAGATCTGCAGTCCATTAATATCAGTGGCAATGGCTTCTGAGATCA GGCACCCGGGGCCAGAGACATATGGGCCATACCCCTTAACCCCGGGATGCAAGGTAGCAT GGGTCCAAGAGACATGGAAGAGAGAGCCATGAAGC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_138473 unedited NGGGGGGAAGGGGNNNNNNNNNNNNNCTCCCCCNNNTTTTCTTACACCCGCCCGTTGNCG CAAAGGGCGGTAGGCGTGTACGGTGGGNAGTCTATATAAGCAGAGCTCATTTAGGTGACA CTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATTCGGCACGAGGCTTGC CTCGTCAGCGTCCGCGTTTTTCCCGGCCCCCCCCAACCCCCCCGGACAGGACCCCCTTGA GCTTGTCCCTCAGCTGCCACCATGAGCGACCAAGATCACTCCATGGATGAAATGACAGCT GTGGTGAAAATTGAAAAAGGAGTTGGTGGCAATAATGGGGGCAATGGTAATGGTGGTGGT GCCTTTTCACAGGCTCGAAGTAGCAGCACAGGCAGTAGCAGCAGCACTGGAGGAGGAGGG CAGGAGTCCCAGCCATCCCCTTTGGCTCTGCTGGCAGCAACTTGCAGCAGAATTGAGTCA CCCAATGAGAACAGCAACAACTCCCAGGGCCCGAGTCAGTCAGGGGGAACAGGTGAGCTT GACCTCACAGCCACACAACTTTCACAGGGTGCCAATGGCTGGCAGATCATCTCTTCCTCC TCTGGGGCTACCCCTACCTCAAAGGAACAGAGTGGCAGCAGTACCAATGGCAGCAATGGC AGTGAGTCTTCCAAGAATCGCACAGTCTCTGGTGGGCAGTATGTTGTGGCTGCCGCTCCC AACTTACAGAACCAGCAAGTTCTGACAGGACTACCTGGAGTGATGCCTAATATTCAGTAT CAAGTAATCCCACAGTTCCAGACCGTTGATGGGCAACAGCTGCAGTTTGCTGCCACTGGG GCCCAAGTGCAGCAGGATGGGTTCTGGTCAAATACAGATCACTACCAGTGCAAACCAACA GATTATCACAAATCGAGGAAGTGGNAGGCACATCATTGCTGCTATGCCAAACCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_138473 unedited CTCCTTATGTACGCGGCCGCAATTCTANGATCGGTTTTTTTTTTTTTTTTTTCCCCTGAT ACTTAGCTAGGACTTAGGGCAGTTGAGAGGCAGATCATCATGCCTCTACTTTCTTAGCAG AGACAAGGAAGACTGGGGCTGTTTGATAATGAGGAAGGGGAAAATGGGACAAAATACTCC TCTTTCAAATGAACACTGTGGAGAAAATCTTTCTGGGTTTGGGGAAGTCAGAGTAAAGAC TTAGGGGAGTAATAAAGCCAGAGACCCCCCTTCTTTTCCTTTCCCTGCCTTGGATCTAAG GCAAGTGAGCCAAATTTGGAAGAGGGAGTAGGAATTTAAGCTTAAGGCTGCTTAATGTCT TCCTGGGGGCTGGTGTTTTGGGAAGGCAGACAAAGAGAAACAGGTCAACCTCAAAGCTGG TCTGCTCATCCTCTTAGAAGAGGTCTGGTATCTGTGGAGTAGAGCCAAGAGAGGCACCAC AGAGAGCACAGTCTCCCAAACAGAAGTAATAACCGGCTCATATTTTATCAGCAGTTTTCA AAAGCAATGTTTTCCGGGGTTGGAGAAGTGCCATATCTTCCTCCCTTTCCCCAAAGCTAC TGCTGCGACCTTTCTTTCATCCTTGTACATAAGTTACTGGCTCTCTTCTAAGGGAGATAA ATTCTGAGATTTCACATTTTCCCTCTCAACCCCAATAGGCTACCCAGCTTCAGTGAGGGG GAAGGAGAGCTATGCAAAGGGCCCCTCACTTGAGGCAGCTAGACTGTCCTTATTTCCTTG GTACCTGATCTCCAAAACTCTTGATCTGATCCCAAGGTCCAAAAGGCATCAGGNTACTTA CACTGAANACTTNCAAGCAGTAGCACCAGGGCTCCCCCTAAAGCATACAGGNGCCTGATT AGCACCCAGAATGATCCCT
Restriction Sites	NotI-NotI
ACCN	NM_138473
Insert Size	7000 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_138473.2, NP_612482.2
RefSeq Size	7667 bp
RefSeq ORF	2358 bp
Locus ID	6667
UniProt ID	P08047
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Transcription Factors
Protein Pathways	Huntington's disease, TGF-beta signaling pathway
Gene Summary	The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (20)

The use of this cDNA Clones has been cited in the following citations:
Aberrant expression of NKL homeobox genes HMX2 and HMX3 interferes with cell differentiation in acute myeloid leukemia ,Nagel, S;Pommerenke, C;Meyer, C;MacLeod, RAF;Drexler, HG;, PLoS One ,PubMed ID 33048949 [SP1]
Bisdemethoxycurcumin Enhances the Sensitivity of Non-small Cell Lung Cancer Cells to Icotinib via Dual Induction of Autophagy and Apoptosis ,Xiang, M;Jiang, HG;Shu, Y;Chen, YJ;Jin, J;Zhu, YM;Li, MY;Wu, JN;Li, J;, Int. J. Biol. Sci. ,PubMed ID 32226300 [SP1]
Estradiol promotes the progression of ER+ breast cancer through methylation-mediated RSK4 inactivation ,Li, Q;Gao, H;Yang, H;Wei, W;Jiang, Y;, Onco Targets Ther ,PubMed ID 31413588 [SP1]
Investigation of the role of DNA methylation in the expression of ERBB2 in human myocardium ,Quiñones-Lombraña, A;Blair, RH;Blanco, JG;, Gene ,PubMed ID 28735727 [SP1]
Inactivation of PI3-K/Akt and reduction of SP1 and p65 expression increase the effect of solamargine on suppressing EP4 expression in human lung cancer cells ,Chen, Y;Tang, Q;Wu, J;Zheng, F;Yang, L;Hann, SS;, J. Exp. Clin. Cancer Res. ,PubMed ID 26689593 [SP1]
Procollagen Lysyl Hydroxylase 2 Expression Is Regulated by an Alternative Downstream Transforming Growth Factor β-1 Activation Mechanism * ,null, The Journal of Biological Chemistry ,PubMed ID 26432637 [SP1]
CMTM3 Inhibits Human Testicular Cancer Cell Growth through Inducing Cell-Cycle Arrest and Apoptosis ,Li, Z;Xie, J;Wu, J;Li, W;Nie, L;Sun, X;Tang, A;Li, X;Liu, R;Mei, H;Wang, F;Wang, Z;Gui, Y;Cai, Z;, PLoS ONE ,PubMed ID 24586462 [Sp1]
A Feedback Loop Consisting of MicroRNA 23a/27a and the ?-Like Globin Suppressors KLF3 and SP1 Regulates Globin Gene Expression ,null, Molecular and Cellular Biology ,PubMed ID 23918807 [SP1]
Genetic and epigenetic mechanisms collaborate to control SERPINA3expression and its association with placental diseases ,Sonia T. Chelbi, Melissa L. Wilson, Anne-Clémence Veillard, Sue A. Ingles, Jim Zhang, Françoise Mondon, Géraldine Gascoin-Lachambre, Ludivine Doridot, Thérèse-Marie Mignot, Régis Rebourcet, Bruno Carbonne, Jean-Paul Concordet, Sandrine Barbaux, and Daniel Vaiman, Hum. Mol. Genet., Jan 2012; 10.1093/hmg/dds006. [SP1]
Coactivator Function of Positive Cofactor 4 (PC4) in Sp1-directed Luteinizing Hormone Receptor (LHR) Gene Transcription ,Mingjuan Liao, Ying Zhang, Jung-Hoon Kang, and Maria L. Dufau, J. Biol. Chem., Mar 2011; 286: 7681 - 7691 [SP1]
The Predominant WT1 Isoform (+KTS) Encodes a DNA-Binding Protein Targeting the Planar Cell Polarity Gene Scribble in Renal Podocytes ,Julie Wells, Miguel N. Rivera, Woo Jae Kim, Kristen Starbuck, and Daniel A. Haber, Mol. Cancer Res., Jul 2010; 8: 975 - 985 [SP1]
The homeobox gene CHX10/VSX2 regulates RdCVF promoter activity in the inner retina ,Sacha Reichman, Ravi Kiran Reddy Kalathur, Sophie Lambard, Najate Aït-Ali, Yanjiang Yang, Aurélie Lardenois, Raymond Ripp, Olivier Poch, Donald J. Zack, José-Alain Sahel, and Thierry Léveillard, Hum. Mol. Genet., Jan 2010; 19: 250 - 261 [SP1]
-Tocopheryl succinate and derivatives mediate the transcriptional repression of androgen receptor in prostate cancer cells by targeting the PP2A-JNK-Sp1-signaling axis ,Po-Hsien Huang, Dasheng Wang, Hsiao-Ching Chuang, Shuo Wei, Samuel K. Kulp, and Ching-Shih Chen, Carcinogenesis, Jul 2009; 30: 1125 - 1131. [SP1]
Regulation of Prion Gene Expression by Transcription Factors SP1 and Metal Transcription Factor-1 ,Shayne A. Bellingham, Louise A. Coleman, Colin L. Masters, James Camakaris, and Andrew F. Hill, J. Biol. Chem. 2009; 284(2): p. 1291-1301 [SP1]
Protein Kinase C{alpha}-Induced Derepression of the Human Luteinizing Hormone Receptor Gene Transcription through ERK-Mediated Release of HDAC1/Sin3A Repressor Complex from Sp1 Sites ,Mingjuan Liao, Ying Zhang, and Maria L. Dufau, Mol. Endocrinol. 2008; 22(6): p. 1449-1463 [SP1]
The Proinflammatory Cytokine, Interleukin-6, Up-regulates Calcium-sensing Receptor Gene Transcription via Stat1/3 and Sp1/3 ,Lucie Canaff, Xiang Zhou, and Geoffrey N. Hendy, J. Biol. Chem., May 2008; 283: 13586 - 13600 [SP1]
The proinflammatory cytokine, interleukin-6, upregulates calcium-sensing receptor gene transcription via Stat1/3 and Sp1/3 ,Lucie Canaff, Xiang Zhou, and Geoffrey N. Hendy, J. Biol. Chem., Mar 2008; 10.1074/jbc.M708087200 [SP1]
PKC{alpha}-induced Derepression of the Human LH Receptor Gene Transcription through ERK-mediated Release of HDAC1/Sin3A Corepressor Complex from Sp1 Sites ,Mingjuan Liao, Ying Zhang, and Maria L. Dufau, Mol. Endocrinol. published 27 March 2008, 10.1210/me.2008-0035 [SP1]
Peroxisome Proliferator-Activated Receptor {gamma}-Independent Suppression of Androgen Receptor Expression by Troglitazone Mechanism and Pharmacologic Exploitation ,Yang CC, Wang YC, Wei S, Lin LF, Chen CS, Lee CC, Lin CC, Chen CS, Cancer Res. 2007 Apr 1;67(7):3229-38. [SP1]
Phosphatidylinositol 3-Kinase/Protein Kinase C-Induced Phosphorylation of Sp1 and p107 Repressor Release Have a Critical Role in Histone Deacetylase Inhibitor-Mediated Depression of Transcription of the Luteinizing Hormone Receptor Gene ,Ying Zhang, Mingjuan Liao, and Maria L. Dufau, Mol. Cell. Biol.,Sep 2006; 26: 6748 - 6761. [SP1]

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

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SKU	Description	Size	Price
RC209154	SP1 (Myc-DDK-tagged)-Human Sp1 transcription factor (SP1), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 8,624.00
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