Fbx32 (FBXO32) (NM_148177) Human Untagged Clone
CAT#: SC101194
FBXO32 (untagged)-Human F-box protein 32 (FBXO32), transcript variant 2
CNY 2,400.00
CNY 2,950.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | Fbx32; MAFbx |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC101194 sequence for NM_148177 edited (data generated by NextGen Sequencing)
ATGAATATTTTGGAAAAAGTGGTACTGAAAGTCCTTGAAGACCAGCAAAACATTAGACTA ATAAGGGAACTACTCCAGACCCTCTACACATCCTTATGTACACTGGTCCAAAGAGTCGGC AAGTCTGTGCTGGTCGGGAACATTAACATGTGGGTGTATCGGATGGAGACGATTCTCCAC TGGCAGCAGCAGCTGAACAACATTCAGATCACCAGGCCTGCCTTCAAAGGCCTCACCTTC ACTGACCTGCCTTTGTGCCTACAACTGAACATCATGCAGAGGCTGAGCGACGGGCGGGAC CTGGTCAGCCTGGGCCAGGCTGCCCCCGACCTGCACGTGCTCAGCGAAGACCGGCTGCTG TGGAAGAAACTCTGCCAGTACCACTTCTCCGAGCGGCAGATCCGCAAACGATTAATTCTG TCAGACAAAGGGCAGCTGGATTGGAAGAAGATGTATTTCAAACTTGTCCGATGTTACCCA AGGAAAGAGCAGTATGGAGATACCCTTCAGCTCTGCAAACACTGTCACATCCTTTCCTGG AAGGGCACTGACCATCCGTGCACTGCCAATAACCCAGAGAGCTGCTCCGTTTCACTTTCA CCCCAGGACTTTATCAACTTGTTCAAGTTCTGA Clone variation with respect to NM_148177.2 >OriGene 5' read for NM_148177 unedited
TTGATACATCCTTAGGCGGCCGGAATCGCCGAGGTGGCATCGCCCAAAAAACTATGAATA TTTTGGAAAAGTGGTACTGAAAGTCCTTGAAGACCAGCAAAACATTAGACTAATAAGGGA ACTACTCCAGACCCTCTACACATCCTTATGTACACTGGTCCAAAGAGTCGGCAAGTCTGT GCTGGTCGGGAACATTAACATGTGGGTGTATCGGATGGAGACGATTCTCCACTGGCAGCA GCAGCTGAACAACATTCAGATCACCAGGCCTGCCTTCAAAGGCCTCACCTTCACTGACCT GCCTTTGTGCCTACAACTGAACATCATGCAGAGGCTGAGCGACGGGCGGGACCTGGTCAG CCTGGGCCAGGCTGCCCCCGACCTGCACGTGCTCAGCGAAGACCGGCTGCTGTGGAAGAA ACTCTGCCAGTACCACTTCTCCGAGCGGCAGATCCGCAAACGATTAATTCTGTCAGACAA AGGGCAGCTGGATTGGAAGAAGATGTATTTCAAACTTGTCCGATGTTACCCAAGGAAAGA GCAGTATGGAGATACCCTTCAGCTCTGCAAACACTGTCACATCCTTTCCTGGAAGGGCAC TGACCATCCGTGCACTGCCAATAACCCAGAGAGCTGCTCCGTTTCACTTTCACCCCAGGA CTTTATCAACTTGNTCAAGTTCTGAATCCCAGCACATGACAACACTTCAGAAAGGTCCCC CTGCTGACTGGAGAGCTGGGAATATGGCATTTGGACACTTCATTTGTAATAGTGTACATT TTAAACATTGGCTCGAAACTTCANAGATAAGTCATGGAGAAGACATTGNAGGGGAGAAAT GCATTGCTGACTGGGAAATTAAGAAG >OriGene 3' read for NM_148177 unedited
TGGTGTGNATTGGCCCCNGTTAGGGATTTNCTAGTTATTTTTTTTTTTTTTTCTAAACAA ATTTTCACATTGTAAACAAAGCGTCCTATTTTGTATTTGAGTTTTGCCTTTTTCTTCCTA GGGAACTATAAGAGAGTTTCTGTGACCATTAAGAGGACTCCATTTTCACCGCTGAAATAG AATTATTGCCCTTATAGTCTTGCTGGCAAATTGTTAGAAAAAAAGTTTATTTACAGTATT TTGCTTTTCCATACCAATTCTAGTGAGAAGTTCACATTCTTAAATTCCCAGTCAGCAACT GCATTTCTCCCCTCCAATGTCCTCTCCATGACTTATCTCTGAAGTTTCGAGCCAATGTTT AAAATGTACACTATTTACAAATGAAGTGTCCAAATGCCATATTCCCAGCTCTCCAGTCAG CAGGGGGACCCTTCTGAAGTGTTGTCATGTGCTGGGATTCAGAACTTGAACAAGTTGATA AAGTCCTGGGGTGAAAGTGAAACGGAGCAGCTCTCTGGGTTATTGGCAGTGCACGGATGG TCAGTGCCCTTCCAGGAAAGGATGTGACAGTGTTTGCAGAGCTGAAGGGTATCTCCATAC TGCTCTTTCCTTGGGTAACATCGGACAAGTTTGAAATACATCTTCTTCCAATCCAGCTGC CCTTTGTCTGACAGAATTAATCGTTTGCGGATCTGCCGCTCGGAGAAGTGGTACTGGCAG AGTTTCTTCCACAGCAGCCGGTCTTCCCTGAGCACGTGCAGGTCGGGGGCAGCCTGGCCC AGGCTGACCAGGTCCCGCCCGTCGCTCAGCCTCTGCATGATGTTCAGGTGGAGGGCACAA AGCCGGTCATTGGAAGGGAGGCCCTTTAAGGCAGGCCTGGGGATCTGAATGTTGTTCAAC TGCTGCTGCCAGTGGGAAATTCTCCTCCTTCCGATAACCCCCAGTTGAAG |
Restriction Sites | NotI-NotI |
ACCN | NM_148177 |
Insert Size | 1150 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_148177.1, NP_680482.1 |
RefSeq Size | 1212 bp |
RefSeq ORF | 633 bp |
Locus ID | 114907 |
Domains | F-box |
Gene Summary | This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] Transcript Variant: This variant (2) has a shorter and alternate 5' terminal sequence lacking an in-frame translation start codon, as compared to variant 1. It uses a downstream in-frame translation start codon and encodes a shorter isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206217 | FBXO32 (Myc-DDK-tagged)-Human F-box protein 32 (FBXO32), transcript variant 2 |
CNY 2,400.00 |
|
RC206217L1 | Lenti ORF clone of Human F-box protein 32 (FBXO32), transcript variant 2, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC206217L2 | Lenti ORF clone of Human F-box protein 32 (FBXO32), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC206217L3 | Lenti ORF clone of Human F-box protein 32 (FBXO32), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206217L4 | Lenti ORF clone of Human F-box protein 32 (FBXO32), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG206217 | FBXO32 (tGFP-tagged) - Human F-box protein 32 (FBXO32), transcript variant 2 |
CNY 4,000.00 |