TNS1 (AK057328) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MST091; MST122; MST127; MSTP091; MSTP122; MSTP127; MXRA6; PPP1R155; TNS
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK057328, the custom clone sequence may differ by one or more nucleotides TTTCAAGGCGAATGATGGGGAAGGGTTCCCCAGTCCCCACAGTGGCCCCACCTCTGGGCCCTGCACCAGA GCCCTTCTGTGTCACGGCGGGCTGTGCACCCATGCACACACCTACGCACACACAACACTCCGCACTGCAG TATATTCTTGCCAAAGATTTCCTTTAAAAGCAAGCACTTTTACTAATTATTATTTTGTAAATGTTTATCT TCTTCTGTCTTCTCCCTCCCTGAATCTATTTTACTGTTGTTTATTGTTGAATCTGTGTGTCAGCCAGGAG AGCGCTGTCTGGCCTTGAACATGGGCTGGGATGGGAAAGGGTCTGGGAGAAGATGGGCAACAAAGAGCCA GGGAGTCATGGACATCGCAGCGACGCAGACCCCAGCAGGTTCAGTCCCGTGCTGCCACCAGCTGTCCAGC TGGGTGTCTGGAGGGAAGAGGGCAGAGGAGGGTCATGTCCCTTCAGCTGGGGGAGGGGCCCAGTGAGCTC CACGTGGCTTTTTCCCAAAGGGAGCAAGAGGGAAGGATTGGGCGAGAAAACAATGGAGAGGGGACCTGCG AAGGAAAACAGGGAGGAAGTGAGCGGTTTGATCAGCCTGCTATCACGGTGTTCTGGCTCTCTTATTTAGC CAGGCGCTTAAGGGACAGATACATCACATCCTAAGTTTGGGAAAGGCCTTTGACCCATGTCATCTGAGCG TCTCCTCCAGTAGCTCTGAAAGCTGTGGACACCAATGGCCAGGATTCCTTCTCCCCTGGTTTTTGAGGAT CCCTGGGTCTTCTGAGACTGGCCAGGAGAGGGATGGTGGGGCCAGTGGTTGTGTGAAAGCAGGAGGGGCA GCCCTCCTGGACAAGTGTGATCCCCCTATAAACGGCTCTCAGGAGGTTAGTGAGTAGGAGATTCTGCCTT GTTCTGATGAGCCTGTGCAGGGGCTCCAGGGGAGCATGCTGTCCAGGGGGCACAGAAGGGTGGTGAGTGT GATCAAATCTAGTCTCACTCCCACTTTTTAGTCTCACTCCTACTTTTGTCCACCACCCCTGCCTCCTGGA TCTTCTCCCACTTTTTTTTTCAGCTTTAGGACCTGGGGAGATCCTGTGAGTCAAGGCAGACACCCAATCC TGCCCCCACACTCGGGGTCCTCCAAGAGGTTGGGGGGCAGAGTCCCAGAGCAGCCCTTTACCCCAGGTCC AGGCCCTGGAATCCTGAGACTCGCGTTTCCTTGGCCAGTGGTAACACAGGACGTGTGTGCGCATGTGCAA GTGTGGATGTATGTGTGTGCGTGTGTTTTGCTCATTTCTTTAGGGAACTTGGGAGTCGGGGTTGGAGGTG CTGGGCAATGGAACTTCAAATTCAATGTCGCCCAGCAGTGAGGGGAGTCGGGAGGTGAGGCCTGTAGGCC AACCAATTGGTGGAGTCTCAGCGATAGCCCAGGTGAGAAGTGGTTCACCCAGAGGGGCAGGGTGGGGGCC TCGGGCAGATCTGTCCCTCTTGGCACCTCTGTCCTCAAATGTCCAAAATGTTGGAGGACCTCTGTTCATA TCCCACGCCTGGGCTCTTGCCAGCAGTGGAGTTACTGTAGAGGGATGTCCCAAGCTTGTTTTCCAATCAG TGTTAAGCTGTTTGAAACTCTCCTGTGTCTGTGTTTTGTTTGTGCGTGTGTGTGAGAGCACATCAGTGTG TGCAGGCTGTGTTTCCCCATTTCTCTCCTCCCTTCAGACCCATCATTGAGAACAAATGTAAGAAATCCCT TCCCACCACCCTCCCTGCCTCCCAGGCCCTCTGCGGGGGAAACAAGATCACCCAGCATCCTTCCCCACCC CAGCTGTGTATTTATATAGATGGAAATATACTTTATATTTTGTATCATCGTGCCTATAGCCGCTGCCACC GTGTATAAATCCTGGTGTATGCTCCTTATCCTGGACATGAATGTATTGTACACTGACGCGTCCCCACTCC TGTACAGCTGCTTTGTTTCTTTGCAATGCATTGTATGGCTTTATAAATGATAAAGTTAAAGAAAACTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK057328 unedited ATACGACTTACTATAGGCGGCCCGCGATTCGGCACGAGGGGCAGCCCTCCTGGACAAGTG TGATCCCCCTATAAACGGCTCTCAGGAGAGGGATGGTGGGGCCAGTGGTTGTGTGAAAGC AGGAGGGGCAGCCCTCCTGGACAAGTGTGATCCCCCTATAAACGGCTCTCAGGAGGTTAG TGAGTAGGAGATTCTGCCTTGTTCTGATGAGCCTGTGCAGGGGCTCCAGGGGAGCATGCT GTCCAGGGGGCACAGAAGGGTGGTGAGTGTGATCAAATCTAGTCTCACTCCCACTTTTTA GTCTCACTCCTACTTTTGTCCACCACCCCTGCCTCCTGGATCTTCTCCCACTTTTTTTTT CAGCTTTAGGACCTGGGGAGATCCTGTGAGTCAAGGCAGACACCCAATCCTGCCCCCACA CTCGGGGTCCTCCAAGAGGTTGGGGGGCAGAGTCCCAGAGCAGCCCTTTACCCCAGGTCC AGGCCCTGGAATCCTGAGACTCGCGTTTCCTTGGCCAGTGGTAACACAGGACGTGTGTGC GCATGTGCAAGTGTGGATGTATGTGTGTGCGTGTGTTTTGCTCATTTCTTTAGGGAACTT GNGAGTCGGNGTTGGAGGTGCTGGGCAATGGAACTTCAAATTCAATGTCGCCCAGCAGTG AGGGGAGTCGGGAGGTGAGGCCTGTANGCCAACCAATTGGTGGAGTCTCAGCGATAGCCC AGGTGAGAAGTGGTTCACCCAGAGGGCAGGNTGGGGGCCTCGGGCAGATCTGTCCCTCTT GGCACCTCTGTCCTCNAATGTCCCAAATGTTGGAGGACCTCTNGTCATATCCCACGCCTG TGCTCTTGCCACANTGGAGTACTGTANAGGGATGTCCCAGCTTGTTTCCATCAGGNTTAA CTGTTGAACCTCTGGGCCGGGGTTTGGTTGCGCGGGGGGAAACCAATATGGTGCCGGCTG GTTTCCCTTTCTTCC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK057328 unedited NAAATACTNTGNNACCGCGGCCGCTTTCTAGNGATCGGTTTTTTTTTTTTTTTTTTCTTT AACTTTATCATTTATAAAGCCATACAATGCATTGCAAAGAAACAAAGCAGCTGTACAGGA GTGGGGACGCGTCAGTGTACAATACATTCATGTCCAGGATAAGGAGCATACACCAGGATT TATACACGGTGGCAGCGGCTATAGGCACGATGATACAAAATATAAAGTATATTTCCATCT ATATAAATACACAGCTGGGGTGGGGAAGGATGCTGGGTGATCTTGTTTCCCCCGCAGAGG GCCTGGGAGGCAGGGAGGGTGGTGGGAAGGGATTTCTTACATTTGTTCTCAATGATGGGT CTGAAGGGAGGAGAGAAATGGGGAAACACAGCCTGCACACACTGATGTGCTCTCACACAC ACGCACAAACAAAACACAGACACAGGAGAGTTTCAAACAGCTTAACACTGATTGGAAAAC AAGCTTGGGACATCCCTCTACAGTAACTCCACTGCTGGCAAGAGCCCAGGCGTGGGATAT GAACAGAGGTCCTCCAACATTTTGGACATTTGAGGACAGAGGTGCCAAGAGGGACAGATC TGCCCGAGGCCCCCACCCTGCCCCTCTGGGTGAACCACTTCTCACCTGGGCTATCGCTGA GACTCCACCAATTGGTTGGCCTACAGGCCTCACCTCCCGACTCCCCTCACTGCTGGGCGA CATTGAATTTGAAGTTCCATTGGCCAGCACCTNCAACCCCGACTCCAAGTTCCCTAAAGA AATGAGNCAAACACACGCACACACATACATCCACACTTGCACATGCGCACACACGTCCTG TGTTACCACTGGCCAAGGAAACGCGAGTCTCAGGATTCANGGGCCTGGACCTGGGTAAAG GNNCTGCTCTGGACTCTGCCCCCCACCTT
Restriction Sites	NotI-NotI
ACCN	AK057328
Insert Size	1400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK057328.1
RefSeq Size	2028 bp
RefSeq ORF	2028 bp
Locus ID	7145
Protein Families	Druggable Genome
Gene Summary	The protein encoded by this gene localizes to focal adhesions, regions of the plasma membrane where the cell attaches to the extracellular matrix. This protein crosslinks actin filaments and contains a Src homology 2 (SH2) domain, which is often found in molecules involved in signal transduction. This protein is a substrate of calpain II. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

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