ZNF313 (RNF114) (AK097265) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	PSORS12; ZNF313
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK097265, the custom clone sequence may differ by one or more nucleotides TGTTGGCCAGGATGGTCTCGATCTCCTGACCTCGTGACCCGCCCGCCTCAGCCTCCCAAAGTGCTGGGAT TATAGGCGTGAGCCATTGCACCCGGTCTCTTCATCTTTATTTATGCATGCATAATGTGGTTTTTGGTTTT GTTTTGTTTTTTTTCTGAGACAGGGTCTGGCTTTGTTGCCCGGGCTGGAGTGCAGTTGCGTGGTCTCGGC TCGCTGAAACCTCTGCCTCCCGGGCTCAAACCATCCTCCCACCTCAGCCTCTCAAGTAGCTGGGACTACA GGCATGTGTCACCACGCCTGCCTAATTTTTTTGTATTTTCAGTAGAGACGAGTTTTGCATGTTGCCCATG CTGATCTCGAACTCGGGAGCGTGAAGTGATGGCCCACCTCAGCCTCCCTCAGGATTACAGGTGTGAGCCA CCGTACTCCGCCCATGATGGGCTTTTTGTTTGTTTTACTGTATGCTTTTGCTTTTCTCCATTTTCAAGAT TAGTGATTGTACTGTTGGCATGCCTCAGAAAATGTTTTTCTTTCCTTTATCTTCCCTCTTTTCTTCAATT CTTAAAACTTTCTTTCTTTTTAGAAAACCTGTAATGAAGCACTATTCTCTGGGTTTTCTTCTCTTTCTGT TTTACTGTTGCACAGCCTAGATTTTATATGAAAGGCTGGGCCGGGCGTGGTGGCTCACGCCTGTAGGCCC GGCACTTTGGCAGGCTGAGGCGGGCGGATCACTTGAGGTCAGGAGTTCGAGACCAGCCTGGCTAGCGTGG TGGAACTCCGTCTCTACTGAAAATATGAAAATTAGCTGGGCCCGATGGCACACACCTGTGGTCCCAGCTA CTCAGGAGGCTGAGGTGGGAGAATCACTTGGGCCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATGGTGCC ACTGCACTTCAGTCTGGGCGACAGAGTGAGTGAGACTCTGTCTCAAAAAAAAAAAAAAAGAGAATTTAGA TTTTAAAGATTATTTCTCAGAGGCTGGTGATAGATACTTTAACCAGGGTTAGTGGCTTTTATATGTTTTA CCTAAAGGATCACTTTTCTCTGCTGCACATAACAGAATGTGGTTTTTGGCTTGCTGTAAGGAGACTAGTT TAAATGCTAGTATAGAAAACTTTTAAAAACATCCTAGATTTGGAGCTTGTCTGTGAGCAGACAGCCTATC ATGAATAGGAGCAAACCTCTAGGAGAGTTATTTTAGAGTGGAAGTGGATATCCTGATTTAGTCGAATAAA ATAGCAGCCTTGGTATTAATGGGAAATCTGCAATAATCAGGGTTTCAGCCCCATCAGCGTAGTCAGGAGT TTGGTGAGATTATTTGCCAGGGTGGTGTAGGTCTAGTTTTCTTTGTGCCCTTGTTTGGGACTATATTTTG CCTGTTGCAAGGTCCTCACTAACTTGTGGGCTCTGATTCTTCCTATTTGAGTTCTTCCTGTCCAAGATCC GGTCCCACGTGGCTACTTGTTCCAAATACCAGAATTACATCATGGAAGGTGTGAAGGCCACCATTAAGGA TGCATCTCTTCAGCCAAGGAATGTTCCAAACCGTTACACCTTTCCTTGTCCTTACTGTCCTGAGAAGAAC TTTGATCAGGAAGGACTTGTGGAACACTGCAAATTATTCCATAGCACGGATACCAAATCTGTGGTTTGTC CGATATGTGCCTCGATGCCCTGGGGAGACCCCAACTACCGCAGCGCCAACTTCAGAGAGCACATCCAGCG CCGGCACCGGTTTTCTTATGACACTTTTGTGGATTATGATGTTGATGAAGAGGACATGATGAATCAGGTG TTGCAGCGCTCCATCATCGACCAGTGAGCAGAGTCCGTGCTTTCTATCTGTCTCATGTTACAGAGCTTCC ATTACATATTAAACGTGAAATCTATG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK097265 unedited GGGGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGGGATAC TTTAACCGGGTTAGTGGCTTTTATATGTTTTACCTAAAGGATCACTTTTCTCTGCTGCAC ATAACAGAATGTGGTTTTTGGCTTGCTGTAAGGAGACTAGTTTAAATGCTAGTATAGAAA ACTTTTAAAAACATCCTAGATTTGGAGCTTGTCTGTGAGCAGACAGCCTATCATGAATAG GAGCAAACCTCTAGGAGAGTTATTTTAGAGTGGAAGTGGATATCCTGATTTAGTCGAATA AAATAGCAGCCTTGGTATTAATGGGAAATCTGCAATAATCAGGGTTTCAGCCCCATCAGC GTAGTCAGGAGTTTGGTGAGATTATTTGCCAGGGTGGTGTAGGTCTAGTTTTCTTTGTGC CCTTGTTTGGGACTATATTTTGCCTGTTGCAAGGTCCTCACTAACTTGTGGGCTCTGATT CTTCCTATTTGAGTTCTTCCTGTCCAAGATCCGGTCCCACGTGGCTACTTGTTCCAAATA CCAGAATTACATCATGGAAGGTGTGAAGGCCACCATTAAGGATGCATCTCTTCAGCCAAG GAATGTTCCAAACCGTTACACCTTTCCTTGTCCTTACTGTCCTGAGAAGAACTTTGATCA NGAAGGACTTGTGGAACACTGCANATTATTCCATAGCACGGATACCAAATCTGTGGTTTG TCCGATATGTGCCTCGATGCCCTGGGGAGACCCCAACTACCGCAGCGCCAACTTCAGAGA GCACATTCAGCGCCGGCACCGGTTTTCTTATGACACTTTTGTGGATTTATGATGTGATGA AGAAGACATGATGAATCANGTGTTGCAGCGCTTCATCATCGACCATTGAGCAGAGTCCGT GCTTGCTATCTGT
Restriction Sites	NotI-NotI
ACCN	AK097265
Insert Size	2600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK097265.1
RefSeq Size	1916 bp
RefSeq ORF	1916 bp
Locus ID	55905
Gene Summary	E3 ubiquitin-protein ligase promoting the ubiquitination and degradation of the CDK inhibitor CDKN1A and probably also CDKN1B and CDKN1C. These activities stimulate cell cycle's G1-to-S phase transition and suppress cellular senescence. May play a role in spermatogenesis.[UniProtKB/Swiss-Prot Function]

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