SHPRH (AK094944) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA545I5.2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC101829 sequence for AK094944 edited (data generated by NextGen Sequencing) ATGGAATTTGCACAAATCAGTCGTGTTAAGACATTTCAGGAGAACCTTTCAGCATTTAAA CGTGATCCCCAAATCAATATTTTGCTGCTGCCCCTGCACACAGGTTCTAATGGATTAACT ATCATTGAAGCAACTCATGTTCTCTTGGTGGAGCCCATATTGAACCCTGCCCATGAGCTT CAGGCCATAGGGAGGGTGCACCGAATTGGACAGACAAAACCTACTATTGTACACAGATTC TTAATTAAAGCAACAATAGAAGAAAGAATGCAGGCAATGCTGAAAACTGCTGAGAGAAGT CACACGAACTCATCAGCAAAGCATTCAGAGGCCTCTGTCTTGACTGTGGCTGACCTGGCA GACCTATTTACCAAAGAAACTGAAGAGCTTGAATGA Clone variation with respect to AK094944.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK094944 unedited CGTCAGAAATTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTCGTTTTC TCAACGTGGCAAGATGTATTAGATATTATTTCAAAAGCTCTTACTGACAACAACATGGAA TTTGCACAAATCAGTCGTGTTAAGACATTTCAGGAGAACCTTTCAGCATTTAAACGTGAT CCCCAAATCAATATTTTGCTGCTGCCCCTGCACACAGGTTCTAATGGATTAACTATCATT GAAGCAACTCATGTTCTCTTGGTGGAGCCCATATTGAACCCTGCCCATGAGCTTCAGGCC ATAGGGAGGGTGCACCGAATTGGACAGACAAAACCTACTATTGTACACAGATTCTTAATT AAAGCAACAATAGAAGAAAGAATGCAGGCAATGCTGAAAACTGCTGAGAGAAGTCACACG AACTCATCAGCAAAGCATTCAGAGGCCTCTGTCTTGACTGTGGCTGACCTGGCAGACCTA TTTACCAAAGAAACTGAAGAGCTTGAATGAACTACACTTGATTCATTCCATGGACTTTAG TGTATTAATAAACTTTCATAGCTGTAGAGCAAAGTTACAAGTTTTAAAAACCCAGTAGAT AACAGTAACACTGTTCCTAGTTGAATGAATTTGTTTATTGTTCTGGTTATACAGTGGCTT AGTACTTATTGAGTCCTTTTCATATACTGTTTCCAAAGATCTATAAAGATTCTTAATTTT ACACTCCTAATAAAAACATTATTTTTTTGTCCCANATAGTATCTATATCTGATGATGCAN GGGAATTAAGAGAGTAATGTGTACTTTTCTTATGATAAGATCACTCATAANACAGTTGGG AATAAGAAAGTAACTAGTCTAGTTTTATATGACCTGGTATATTGAACAAACTAGATCTCA GTNTGGGAGGGCCTTNCAGTATCTTAGCACA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK094944 unedited TTAGCTNTGNNACCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTCATTCC CTAAAAATTTATTTAATGGATATCTTACAGAAAATAAATAAAAACAGTTTTTGTCTAATA CAGATAGGTTTTTTTATATATATGTAATTTGATTTTACAAAATTGAAGCATATCAAATTG TTTTCTCTAAAACATAGCTCAGAAAGGAATAGTAGAAAACAAACACAAAGAAATACTGAA ATAGTCTCATTTTTAAATTATAAGCATGGGCCTTCTGAATCTCAGCATGACATGTTATTA GTAAAAGAATGTAAGATATGGAGTCAGGGGACCTGGGCTCCACTGTTTACTTGCTACATG TCCTAAGAAAGTTTCAATATATCTGAACTTCAGTTCTCTACCTTTAAAACAGTAAAGATA TTAATTATATCAACTTCACAAGATTGTTGGGTAAATTAAACACGTAAATGTACATGAAAG CTCTTTTTAATATGTGAAGAGCTATAAAAATAACAGTTTGATTTAATTTCATATAAAAGA ATCAATGGATACCTCCTTTTAACTGAAGAATAAGCACCAAAAGCATGTGTGTGTTTTCTT CAGTTGAATTACATCACTCTATAAACCTAGAGTAAGAAAATGCACCTTAACTAAGCACCA AAGCCTCTCGGACAAATGGGATCTAGGGAATGGTTCTATGAGTTTTCCAGGCAGCTAACT GGGAGCTAATAAGTCCTGAGGGTCAAAGAGGGTCCTTNTAAACTCTGAAAGCTCAAAAAA AGGAAGCCAAATAAGACATTTGTATTTACATCAATGGCTAGGATGTAACCAGCTACTCCC TTATTCAGCATTTCAAAGTCCCATTATTCCTCACAGAAGAAGAAACACTTTCGTATTATC ACTGCNACGATTTGCTTAG
Restriction Sites	NotI-NotI
ACCN	AK094944
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK094944.1, BAC04459.1
RefSeq Size	2335 bp
RefSeq ORF	396 bp
Locus ID	257218
Domains	helicase_C
Protein Families	Druggable Genome
Gene Summary	SHPRH is a ubiquitously expressed protein that contains motifs characteristics of several DNA repair proteins, transcription factors, and helicases. SHPRH is a functional homolog of S. cerevisiae RAD5 (Unk et al., 2006 [PubMed 17108083]).[supplied by OMIM, Mar 2008]

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