SFRS5 (SRSF5) (BC029984) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HRS; SFRS5; SRP40
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC029984, the custom clone sequence may differ by one or more nucleotides CAGAAAATCGTCTTATAGTTGAGAATTTATCCTCAAGAGTCAGCTGGCAGGTTTGTTGAAATACAGTTTT GAGTTATTTTGATGTGGCTTTTTAAAAAAGTTAATGGGTAGCTAATGTTGTATTGTTTTATTAAAAGTAG TTTTAAATTAATGGATTTAATGGAATTGTAATTTTAAACATTTAATTAAATGTAATTTGGGGGATATTTT GAACTTTAATATTAGTGATCAAATGTAAATGTTTTGAGGATATTTTTCTTGTTTTTTAAAATCAATTTTT AACCAAATATTAAACCCTTTATTTGCCAGCCTGTCTGTGTCGTTGGCCTTATGACGAGGAGTGCCTGTGG GTTATCCTAATCGTTGTCTTGGTCACTCTTGGTTGGGCCTGGTTGACTTTGCCAGTCAGCTCCTACAGTG ATCAGTTGGCCACCTCTAGATCTGTGTTTGCCGGTCTAGACGTTATCGGTGCACTTCCTTGAAGTGCCAG GTTGCAGCGATCCCAGAGCGTTGATAACGTGATCTGATCCCTAAGTTGAGACCTGTCTTCCTGTAACGCT TCCGAATAAGAGGTCCGGTCGAAAAGAGTTGAAAGATACGAAATTAGGTGGTCGTTGGAATCCTGATCGC AGTAAAGTGGTCCTTGGTAACTGCTCGAGTAGAACATGTCTGCATCCGCCAATAGTCCGCTAATAGGGAG GGCTGTGCGATTAAAGGCTTCCATCGATTGGGTAGTGTCCTTCAAGTGGGTGGCGAAGAGCCAGTCGGGC ATATGTCATGAAGGTTTCTCCGACCGATTAATGGTTTGCTGCTTGACTAGCCTAGGGAAATAATAATAAA GGTAAAGTAAACTTTTTTAATGACATATGGGGCACAAAATAACTGGTGTCTTGTAAATGTTCTGTTTTTT TAAATAAAACTTCTCTACTTTAGTCTTTACTTTAAAAATATGTACTGTTTCCTTTTTTGTTGTTTTTTTG TTTGTTTTTTTTTCTTTTGTACTGAGCTCAGCATAGACTAATACTACCTTAATGTTAAAATCTGAATTTC TTTTAGCATTTTGCTTAAAAGCAATATGCTATTTGCTTATTCCGTGCCCTGACATAGATAATTTTTGAAT TCTGATAGAATACAAGTGTGGTAAATGCCATGTTTGTACTTTATCTAACATCTTCTCTTTCAGTAGTCTT GTTTTTTTTATATCATGTGATTGTTTGTGTGTCCCCTTTCCTCTTCTTTGCTTAACACAATTATCTTGTG TTAAGGATCTCAAAGATTTCATGAGACAAGCTGGGGAAGTAACGTTTGCGGATGCACACCGACCTAAATT AAATGAAGGGGTGGTTGAGTTTGCCTCTTATGGTGACTTAAAGAATGCTATTGAAAAACTTTCTGGAAAG GAAATAAATGGGAGAAAAATAAAATTAATTGAAGGCAGCAAAAGGCACAGTAGGTCAAGAAGCAGGTCTC GATCCCGGACCAGAAGTTCCTCTAGGTCTCGTAGCCGATCCCGTTCCCGTAGTCGCAAATCTTACAGCCG GTCAAGAAGCAGGAGCAGGAGCCGGAGCCGGAGCAAGTCCCGTTCTGTTAGTAGGTCTCCCGTGCCTGAG AAGAGCCAGAAACGTGGTTCTTCAAGTAGATCTAAGTCTCCAGCATCTGTGGATCGCCAGAGGTCCCGGT CCCGATCAAGGTCCAGATCAGTTGACAGTGGCAATTAAACTGTAAATAACTTGCCCTGGGGGCCTTTTTT TAAAAAACAAAAACCACAAAAATTCCCAAACCATACTTGCTAAAAATTCTGGTAAGTATGTGCTTTTCTG TGGGGGTGGGATTTGGAAGGGGGGTTGGGTTGGGCTGGATATCTTTGTAGATGTGGACCACCAAGGGGTT GTTGAAAACTAATTGTATTAAATGTCTTTTGATAAGCAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC029984 unedited AGTATTTTGTAATACGACTCACTATAGGCGCGGCCGCGATTCGGCACCGAGGGCCTAGGG NAAATAATAATAAAGGTAAAGTAAACTTTTTTAATGACATATGGGGCACAAAATAACTGG TGTCTTGTAAATGTTCTGTTTTTTTAAATAAAACTTCTCTACTTTAGTCTTTACTTTAAA AATATGTACTGTTTCCTTTTTTGTTGTTTTTTTGTTTGTTTTTTTTTCTTTTGTACTGAG CTCAGCATAGACTAATACTACCTTAATGTTAAAATCTGAATTTCTTTTAGCATTTTGCTT AAAAGCAATATGCTATTTGCTTATTCCGTGCCCTGACATAGATAATTTTTGAATTCTGAT AGAATACAAGTGTGGTAAATGCCATGTTTGTACTTTATCTAACATCTTCTCTTTCAGTAG TCTTGTTTTTTTTATATCATGTGATTGTTTGTGTGTCCCCTTTCCTCTTCTTTGCTTAAC ACAATTATCTTGTGTTAAGGATCTCAAAGATTTCATGAGACAAGCTGGGGAAGTAACGTT TGCGGATGCACACCGACCTAAATTAAATGAAGGGGTGGTTGAGTTTGCCTCTTATGGTGA CTTAAAGAATGCTATTGAAAAACTTTCTGGAAAGGAAATAAATGGGAGAAAAATAAAATT AATTGAAGGCAGCAAAAGGCACAGGTCAAGAAGCANGTCTCGATCCCGGACCAGAAGTTC CTCTTAGTCTCGTAGCCGATCCCGTTCCCGTAGTCGCAAATCTTACAGNCCGTCAAGAAG CANGAGCANNGAGCCGAGCCCGAGCAAGTCCCGTTCTGTTAGTANGTCTCCCGTGCCTGA GAAGAGCCCAGAACGTGNTCTTCAGTAGATCTAGTCTCCAGCATCTGTGGATCGCCAAAG TCCCNGTCCGATCAAGTCCAGATCAGTGACATGGCATTAAACTGTAATACTTNCCTGNGG NCCTTTTTTTTAAAACAAACACAAANTNCCAACCTACTGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC029984 unedited TAACTGTGNACCGCGGCCCGCTATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTAGTTTTATAATATTTTATTATCAATAACAAACAGCTGCTCTAATGTGTCT GTCCAGCAGCTGTTAAAGAGTGGAGGACACCCTTGACCCTAACAAGGAAAACAAATTAAG CCTTTATGTACAAGCAAATTTAGAGCTCTTTTAAGTGTCCAAAGCTATTAATTAGTTTAA TTAAGGCATTAAACTAATTCTGAATTAACATTTTTATAACCAAGAACTAAAATGTTCAAA TTTTTTTCTAGTACAAAAAAATTAAATTTGCTTTAGTTATAAAAGAGCTCTGTCAATATA CACAAACTATATACTTCAGACATTCACAAAAATGTGAGCAGAAGGCTTATCAAAAGACAT TTAATACAATTAGTTTTCAACAACCCCTTGGTGGTCCACATCTACAAAGATATCCAGCCC AACCCAACCCCCCTTCCAAATCCCACCCCCACAGAAAAGCACATACTTACCAGAATTTTT AGCAAGTATGGTTTGGGAATTTTTGTGGTTTTTGTTTTTTAAAAAAAAGGCCCCCAGGGC AAGTTATTTACAGTTTAATTGCCACTGTCAACTGATCTGGACCTTGATCGGGACCGGGAC CTCTGGCGATCCACAGATGCTGGAGACTTAGATCTACTTGAAGAACCACGTTTCTGGCTC TTCTCAGGCACGGGAGACCTACTAACAGAACGGGACTTGCTCCGGCTCCGGGCTCTGCTN CTGGCTCTTGACCGGCTGTAAGATTTGCGACTACGGGAACGGGATCGGCTACNAGACCTA GAGGAACTCTGGGTCCGGATCGAGACTGGGCTCTGACTGTNGCCTTTGCTGCCTCAATAA TTTATTTTCTCCATTTATTTCTTCCAGAAGATTTCA
Restriction Sites	NotI-NotI
ACCN	BC029984
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC029984.1
RefSeq Size	1959 bp
RefSeq ORF	1847 bp
Locus ID	6430
Protein Pathways	Spliceosome
Gene Summary	The protein encoded by this gene is a member of the serine/arginine (SR)-rich family of pre-mRNA splicing factors, which constitute part of the spliceosome. Each of these factors contains an RNA recognition motif (RRM) for binding RNA and an RS domain for binding other proteins. The RS domain is rich in serine and arginine residues and facilitates interaction between different SR splicing factors. In addition to being critical for mRNA splicing, the SR proteins have also been shown to be involved in mRNA export from the nucleus and in translation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

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