CHD3 (AK096555) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Mi-2a; Mi2-ALPHA; ZFH
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK096555, the custom clone sequence may differ by one or more nucleotides TTAGCCTCTAGGACTTGTGCAAGCCAACCCTCATCCATGTCTGATAGCATTCACATCCGTGCCCAATAGA GGGACCTGCCCCAGCCTCTGTGTCCCCTCCACACAGTGGGGCCTCTTCACTGGCAGTGGAACTGCATGCC TGCCATACTGCTTAACATCATCCAGTTAGGGGCCTTGGACTGTGAATGCACCATACAGATCCCTGGCTGT GATCAAACAAATCACATTCCCTCACATGTGTTTCACCCCATAAGGCAGAAACTACCACCCATCCAACCCT CTGGCCTTACCCCATGAAACTTCCCTTTGACCTTCGACCTTTCCTCCTACCCCCTCTCACCTTGCCATTG ATAGAGATTATATCCCAAGGCCCTCTAACCATCTAATCCTGCCTGGTGTAGTCACTGACTCCTGGCCCCT TTGATCCCTGTTCTAATTCCTTGAATCTGTAATACTGACCTTCTAACCTCCCTCTCCCCTTACATATTAA AACCGTGATTCCTTAAAGCTTTGACACTTACCACCTCCCATGCCTCCTGACTATTTCCTCCCCATCATCC CCAACCCCAAACCTTGCTCTTCCAGTATGAGATGTTCTGACAACTCCCCGCCCCCATGTCTTCCAATGTC CCCCCATCTTCCTTTCCTCCATGCTCCCTTTTTCTTTCTTCTTTCTCTCCATCTGTCTTCTGTGGTAATC TGGTCTCTCTGTCTCTTTTCCTGACACTTTTTCTTTTCCCCTGAGTTGCTTCTGTTTTCCATCTACTTCT TTTACTTTCTTGATCTCTGGTTCTTTGACATCTGTGTTCTCCTCTCTCGCTCTTTTTCTGCCTGTATCTG TCCATCTGATGCCTCTCTTTTCCTGGCTCCATCTCTGTATTTCCCTGTCTTTCTCTTGCCCTTCTTTCTC TGTGGCCCGGGCCCCAGTTCTGAACCAGCTGGAGGAGTTGCTGAGCGACATGAAGGCGGACGTGACCCGC CTGCCAGCCACGCTGTCCCGAATACCCCCCATCGCAGCCCGCCTTCAGATGTCCGAGCGCAGCATCCTCA GCCGGCTGGCCAGCAAGGGCACGGAGCCTCACCCCACACCGGCCTACCCGCCGGGTCCCTACGCTACACC TCCGGGGTACGGGGCGGCCTTCAGCGCCGCACCCGTAGGGGCCCTGGCCGCCGCAGGCGCCAATTACAGC CAGATGCCTGCAGGGTCCTTCATCACAGCCGCCACCAACGGCCCTCCAGTGCTTGTGAAGAAGGAGAAGG AAATGGTGGGGGCATTGGTGTCAGACGGGCTGGATCGGAAGGAGCCCCGAGCCGGGGAGGTGATCTGTAT AGACGACTGACTGGATCCCAGGCCTGCCCTTCACCCAGGCCCCGTCCCCGAGGCCGACCCCCAGCTCAAG CGCTGGGGCCTGCTGCCAGCCCTCCACCTTCCCCACCCCTTGGGCCATCACTGGGCTAGGAACCCCTTTG CCCCTCTCTGCAGCTCCTCTCTTCAAGAAGGGCCCTTTGTCTTTCTCCACTCCCACACACCTTTCCCACC AAGCCTTGAAGACTGTGCTGGTGAGAAGAAGTCTGGGTGGGAGATGGCTGGCAGGGTCTTCCAAGTACCT TCCTCCCACACTGCCAAGTATACACAACTTCCCAGTAAATGGTTGTGGGGAGGAAAGAGGTGGAGCCTCC CCAGCCGTTTCCCTGCAGAATCAGCTCTGTCTCATGTGGAAGTGGAGAATCAGCCTTGCCTGGCCTTTAG GAACTTTTGTGGGGAAGAGAGCTTTGAAGAGAGGAGGGGGACTTTAGAGAGGGATGAAAATGAGCCCTGG GAGGGAGGAAGGGACGAGGAGGGGTGGCTGCATGTTACCGTCCCCTACCTCTCCCCACGTGGAGGGTGGA GCAGTTATGAGGGAGGAAGTCAACTGCTGTTCAGCCTCAGAATAAAGGTGCCGTTCACTGGCTCAGTTAC CTCCTGTGTACCGGCATCTTGTGTTGGGAATGTTCCCCCCTCCCTAGGGACCAAGGACCACCCCTACAAA AAGAGTAATGGTTGGGTGATACTCCCTCAAGCCAAAGAGGAGCTCCCCAACCTGTTCTAGGGACCCAGGT AACCTAGAAGGGTGGGAGAGAATACAATGGGCCAGATGTGGTGGAAGCCCAGCTCTGGGGCTCAGGTTCC TGGAAGACTTCTACTACCCTCCCTCCTCAAGGCCTGGATACAGACTAAATTTGTATAAGTCAGGCAGGGG ACCTAGTCAGGGTCTTGGGAGCTACCTTGTCGTTGGGACCAGAGCAAAATAGTGGAGGGCAGGCTAGGGA AATGTGGGCACATCCCCCCTCCCAGGAGGGGCCGGGGAGAGTGGCAGTTTGCATGGCGAACCCCCCACTT CCTCTTTGCTGCCCATTCACTTTCTTGCTGCCCCTTTCCCAGTCTCTCTTCACACCCACTCCTGGTCTGT CCTGATCCCCTCTTCTGTATCAGGTTTATTGGTTGTACATATAAATTATACTTTCCTTTC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK096555 unedited TCCCATTTGTATACGACTCACTATAGGCGGCCGCGAAATTCGCACGAGGCAGCCGCCACC AACGGCCCTCCAGTGCTTGTGAAGAAGGAGAAGGAAATGGTGGGGGCATTGGTGTCAGAC GGGCTGGATCGGAAGGAGCCCCGAGCCGGGGAGGTGATCTGTATAGACGACTGACTGGAT CCCAGGCCTGCCCTTCACCCAGGCCCCGTCCCCGAGGCCGACCCCCAGCTCAAGCGCTGG GGCCTGCTGCCAGCCCTCCACCTTCCCCACCCCTTGGGCCATCACTGGGCTAGGAACCCC TTTGCCCCTCTCTGCAGCTCCTCTCTTCAAGAAGGGCCCTTTGTCTTTCTCCACTCCCAC ACACCTTTCCCACCAAGCCTTGAAGACTGTGCTGGTGAGAAGAAGTCTGGGTGGGAGATG GCTGGCAGGGTCTTCCAAGTACCTTCCTCCCACACTGCCAAGTATACACAACTTCCCAGT AAATGGTTGTGGGGAGGAAAGAGGTGGAGCCTCCCAGCCGTTTCCCTGCAGAATCAGCTC TGTCTCATGTGGAAGTGGAGAATCAGCCTTGCCTGGCCTTTAGGAAACTTTGTGGGGAAG AGAGCTTTGAAGAGAGAGGGGGACTTTANAGAGGGATGAAAATGAGCCCCTGGAGGGAGG AANGGACCCAAGAGGGGTGGCTGCATGTTACCGTCCCCTACCTCTTCCCACGTGGAAGTT GGGAGCAGTATGAAGGAGGAAGTCAACTGCTGTTCAGCCTCACAAATAAAGTGCCGGTCA CTGGCTCAAGTACTTCCTGTGGCCCGGCATCTTGTGTTTGGGAAGGTTCCCCCCTCCTAT GGACCAAAGAGCCACCCTACAAAAAAAAGTATGGGTGGGTGATACTTCCTCCAGCCAAAA AGAGCTCCCCACCTGTCTTAGGACCCACGTAACTAAAAGGTGGGAGAAATCCAGGGCCCC AATTGGGC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK096555 unedited NTTGGTACTCTATGNACCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTG AAAGGAAAGTTAATTTATATGTACAACCAATAAACCTGATACAGAAGAGGGGATCAGGAC AGACCAGGAGTGGGTGTGAAGAGAGACTGGGAAAGGGGCAGCAAGAAAGTGAAGGGGCAG CAAAGAGGAAGTGGGGGGTTCGCCATGCAAACTGCCACTCTCCCCGGCCCCTCCTGGGAG GGGGGATGTGCCCACATTTCCCTAGCCTGCCCTCCACTATTTTGCTCTGGTCCCAACGAC AAGGTAGCTCCCAAGACCCTGACTAGGTCCCCTGCCTGACTTATACAAATTTAGTCTGTA TCCAGGCCTTGAGGAGGGAGGGTAGTAGAAGTCTTCCAGGAACCTGAGCCCCAGAGCTGA GCTTCCACCACATCTGGCCCATTGTATTCTCTCCCACCCTTCTAGGTTACCTGGGTCCCT AGAACAGGTTGGGGAGCTCCTCTTTGGCTTGAGGGAGTATCACCCAACCATTACTCTTTT TGTAGGGGTGGTCCTTGGTCCCTAGGGAGGGGGGAACATTCCCAACACAAGATGCCGGTA CACAGGAGGTAACTGAGCCAGTGAACGGCACCTTTATTCTGAGGCTGAACAGCAGTTGAC TTCCTCCCTCATAACTGCTCCACCCTCCACGTGGGGAGAGGTAGGGGACGGTAACATGCA GNCACCCCTCCTCGTCCCTTCCTCCCTCCAGGGCTCATTTTCATCCCTCTCTAAAGTCCC CCTCCTCTCTTCAAAGCTCTCTTCCCCACAAAAGTTCCTAAAGGCCCAGCAAGGCTGATT CTCCACTTCCCATGAGACAGAGCTGATTCCTGCAGGAAACCGCTTGGGGAGA
Restriction Sites	NotI-NotI
ACCN	AK096555
Insert Size	1300 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK096555.1
RefSeq Size	2510 bp
RefSeq ORF	2510 bp
Locus ID	1107
Protein Families	Druggable Genome
Gene Summary	This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

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