SPAG16 (AK090574) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SZFP41
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK090574, the custom clone sequence may differ by one or more nucleotides TTCAGGTCATCTGGGAGAACTTAAATTCTTCCTTCTGCTATTACAATTCTTCTCACTCAGTTGCCTGCCT CTTTTTTTTTTTTAATTTACTTGTGACTGAGTTGAAGCCCAGCATTTTACTTTCCTTAATGATCTGACTT TCATAAGTTGATAAGTCATGAAATCATTATCTTACATTATATGAGATATGAGATAAATCATAAATCATAT GAAATTGATAACTCATATGAAAACCAACCCCACCATATGTTCACTGTCTACTTGTGTCCATCTTCGTCCC ACGCACCCCACAAGGACCTTCCCCGCCATGTGCCTCAAACATCAGTTCCTGACTCAGGGCCTTTGTATTT GCAGTGCCCGTTGCAAAGAGCACAATTCTTTATTTTATCTGTGGCTTGTCCGTCATGTCTTTTTTTCCAT GACACCTCCACACAGGCCTTCCCTGCCTGCCTGGTTTAAAATTCCAGCCCCTTACCCTTCCTCCTTTATT CTTCTCCCTAGCAATTTCCACCATGCTGCCTGGTGTATTTTCCTCATTTCTCTGTGGCTTCTACACTAGA AAATATTTCCCTTTTGTTCACCACTATGAGTCTAGACCCATGACTGCCATACAGCAGGCATTCCATAAAT GCACCTGTGGAGAGTTGACCCATGCTTGTTGCGTGACTTCTGTCTGATTTTTTTCTGTGTTGCAGCATAA GAAGCGCTAATAGGGAGAACCAACTTGGCTAAAACACACCAGGCTTTTTCACCTCTAGTCTGTACCTGGG CTGTATCCCTTCTCAACCCTAAAGCTAAAAAGTCATTGTGAACCTTTTGGTCTGATGCTAAAGAAGGGAA AACAGGTACAGGAAATCCCATTTGGATGCTTGCTTCCAGGATTTCCTGCCATGATTCCAGAATCCCACAG CTCCAACATGATTGCAAAAGACTCCCTGCTCATTTTTCCTCAGCATGCACAGCGCTGCCCTGTCTCAGTT GCAACTCGACAGAGCCGCATTTACTCCAGAACCCAATCCACACACCTGCTCATCCTGCCCCGAGAGGAGT GCCTGAAGCCAATAGCAGGGAACTAGAGCAGACTTGGGTGGATCTTCATTGGATATTAGGTATCTTGCCC TAGATAGGCAGCAGTGGCCTTACAGATGCTGACAGATGATCTGATTAGATGCACAGTTGCTGGGTGGCGT CTGGGGCCAGTCTATTGGCAGTTCTGGGAGTGGGAACTATTTGGGCTCTGCAAAGATGGTTTCACCTTTA AGAAGGGCAGTATGGGGAGGTGAAGATGGGGCCCAAAGGATAGAACATAGTCAAAGAAGCAGGGCCACAA TGCCCCTTTAATTCCCAAGCCCTAGTTGAGCAGTGCTTGGTGACACATCCCATTACCTCACCTTAGATCA ATGTCTACTGAGCCATCATTAGCTCTGCTTCCTCACTTCTTCACAGTATCTGATGGCCCATCACATGCTG CTCAACATCTTAGACCAATGTCTGCTCACCCATTACCTCCCTTCACCTCATCCAGCACTGTATCTGAAGC TTCACTATTCTTCCTTCAGTTTTGGATATTTTGTGATACAACATTATCCCCAACTTAGGACAATGTCAGC TGACTCTCAGGAGGGTGCCTGGAGATCACCTCTTCCTTCCTAACATACCCTAGAACAAGGTCTGGTCCCC TTCATTATTCCCATTTTACCTTACTCCTAAGTGTATCACTCCTCATGAATTCCAGGAAATATGGAGGAAA ACACATTTCACTGTACATGACTTTCAATAATTTCTCCTTTATATTTATTGTAACGTGATATTTTAGTGCT GACATATATATGTATATGTATGCCCATTTATATAAAAGACTAGTTTTTAGAGAAATTTTAGGTTCACAGC AAAATGGGGCAGAAGGTACAGAGATTTCCCATATATCCCCTACCCTCACATATACACAGCCTCCCCCATC ATCAACATCCCCCTTCAGAGTGGTACATTTGTTACAATTGATGAAACTACATTGACACTATCGGCCAAAG TCCATGGTTTAATTAGGGTTCAGTCTTGATGTGCATTCTGTGGGTTGAGACAAATGAATAATGACATGTA TGCATTATTACAGAAGGATAAAGAGCCGTTTCACTGTCCCAATAATCCTCTGTGTTTCGCCTACTCCTCT AAACTCCTGGCTATTTTCTTTTGTCTCCATAATTTTGCCTCTGAACCATAAAGTATGCAGCCTTTTCAGA TTGATTTGTTTCACTTAGTAATGTGGATTTAAGTTTCTTCCATGTCTTTTCGTAGCTTGCTAGCTCTTTT TTTTTTTTTTTTTTTCTCATTGAATAATATGCTATTGTCTGGAAGTACCATGACTTATTTATCCATTCAC CTACTGAAGGACACCTTAATTGCTTCCAAGTTTTGTTGATTATGAATAAAGATGCTATAAAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK090574 unedited GGGGTGCACATTAGGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGACCTCGTGC CGAATTCGGCACGAGGCCGCCCGCCTCGGCCTCCCAAAGTCCTGGGATTACGGACGTGAG CCACTGCGCCCGGCCCAGCACAGTGTTTTGTACACTCCCACCACCCTCAAGAAAGCCTTT GAGGTTAGGATTTTCAGGTCATCTGGGAGAACTTAAATTCTTCCTTCTGCTATTACAATT CTTCTCACTCAGTTGCCTGCCTCTTTTTTTTTTTTAATTTACTTGTGACTGAGTTGAAGC CCAGCATTTTACTTTCCTTAATGATCTGACTTTCATAAGTTGATAAGTCATGAAATCATT ATCTTACATTATATGAGATATGAGATAAATCATAAATCATATGAAATTGATAACTCATAT GAAAACCAACCCCACCATATGTTCACTGTCTACTTGTGTCCATCTTCGTCCCACGCACCC CACAAGGACCTTCCCCGCCATGTGCCTCAAACATCAGTTCCTGACTCAGGGCCTTTGTAT TTGCAGTGCCCGTTGCAAAGAGCACAATTCTTTATTTTATCTGTGGCTTGCCCGTCATGT CTTTTTTTCCATGACACCTCCACACAAGCCCTTTCCCTGCCTGGCCTGTTTTAAAATTCC AGCCCCTTACCCTTTCTCCTTTATTCTTCTCCCTAGCAATTTCACCATGCTTGCCTGCGT ATTTCCCTCATTTCCCTGTGGGCTCTACACTAAAAAATTTTTCCCTTTTGTTCCCCATTT TATGTCTAGACCCATGACTCGCCTACAGAAGGCTTTCCAATAATGCACCTGTGGAGAATT TGACCATGCTTTGTGCGGGACCTACGGCCGAATTTTTTTTGGGTGGACCCTAACATACGC TTTTGGGGAAACCTCCTTG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK090574 unedited TTGGTACATCTATGNACNCGCGGCCGCATGCTAGNGATCGGTTTTTTTTTTTTTTTTTTG TTTATAGCATCTTTATTCATAATCAACAAAACTTGGAAGCAATTAAGGTGTCCTTCAGTA GGTGAATGGATAAATAAGTCATGGTACTTCCAGACAATAGCATATTATTCAATGAGAAAA AAAAAAAAAAAGAGCTAGCAAGCTACGAAAAGACATGGAAGAAACTTAAATCCACATTAC TAAGTGAAACAAATCAATCTGAAAAGGCTGCATACTTTATGGTTCAGAGGCAAAATTATG GAGACAAAAGAAAATAGCCAGGAGTTTAGAGGAGTAGGCGAAACACAGAGGATTATTGGG ACAGTGAAACGGCTCTTTATCCTTCTGTAATAATGCATACATGTCATTATTCATTTGTCT CAACCCACAGAATGCACATCAAGACTGAACCCTAATTAAACCATGGACTTTGGCCGATAG TGTCAATGTAGTTTCATCAATTGTAACAAATGTACCACTCTGAAGGGGGATGTTGATGAT GGGGGAGGCTGTGTATATGTGAGGGTAGGGGATATATTGGGAAATCTCTTGTCCTTCTTG CCCATTTTGCTGTGCACCTAAAATTTCTCTAAAAACTAGTCTTTTATATAAATGGGCATA CATATACATATATATGGTAAGCCTAAAATATCCCGTTGCAATAAATTTAAAGGAGAAATA TTGGAAGCCCTGTACAGTGAAATGTGTTTTCCTCCATATTTTCCGGAATTTATGAGGGAG TGAACCACTTAGGAGTAAGGCAAAAGCGGATAAAGAAGGGGAACAGAACCTGCTCCAGGG TTTTTTAGGGAGGGAAGAGGGAATCGCCAGCACCCTCCCGAGAGTCTACCTGACATTGGC ACTAATGTGGTG
Restriction Sites	NotI-NotI
ACCN	AK090574
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK090574.1
RefSeq Size	2442 bp
RefSeq ORF	2442 bp
Locus ID	79582
Gene Summary	Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM, Jul 2008]

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