ZNRF1 (AK094870) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NIN283
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK094870, the custom clone sequence may differ by one or more nucleotides AAAGACGCGGGTGAGTGTGTGATCTGCCTGGAGGAGCTGCTGCAGGGGGACACGATAGCCAGGCTGCCCT GCCTGTGCATCTATCACAAAAGGTAGGAGGGGTTGGCAAGGTAGCTTAGTGCACCCCACCTCCCAGAGGG GCGGACCCCCATCTCCAGCCATTCTGTCTCCTTTGGTTATGGGATGACCTCTCCCCCGACCTCTGGCTCC GAGCGGGTAAGGGCAGAGGCCGTCAGACAGGTGTCCACTGTGCCGGCGGGAAGGTGCGAGCATCCCAAGT GCAGAGCAGAGTGGAGTTGCCAGAGGTGTGCTCTGGTTTGAGGGCTCAGGTGGTGGGAGCTGATGGGGAG ACCCGTAGAGCCTCACAGGTTGGAGGAGCTTCGGATCCCACTGGGAAGGTTGGTTCTCCGTCTGTCTCCC TGCCTCTTCTTCCTCTACGGGTCCCTCTGCTCCACAGGGGTAGAACATCAATCTGTGCGAGGAAGGCCAG GCAGAGGGTGTACCCACTGCCTTGCACTGGCCTTCTCCCTAGAGGGCCGGGAGGCAGGAAGAGCCATTTC CTGTGGGGCCACAGCACTGGGCACAGTTAAAAGTAGCAGGGCCCAGATATGCCTTGGGACTCCAGTGTGA GCCTCGTCCTTGTTTCCAGCTGGAAGGAAGGCACCCTCTTGCCCAAGACAGGACACTGTGCTGCCTGGGG CCAGCACCTGCTGAATCCTCGAGCCCAGAGCTTCTTCCTGACACTCAGGCCTTTCAAAGCCTGACTCTCA GGGCCACTGCTGCACAGGGTGACAACCGGGCATGGCACGCAGTGTGGCTGGTGCAGAGGAGGCTTAAGGC TGGCCTGGGAGAGATTCTTGGTCTAGCTCCCAGCACGGAGCCTTGCTTGTGTCCCAGGGCGGCACTAACC AGAACCAGAAAAGGAAATCTTTGGCCCTCGGGGAGAATTATGCCAATTATCTGTCTGGTTCCTGTCTTTG AAAATGCCCAGATGTTGGTTTACAAATCAGGAAGCTGTCTTGGGGCAATCTCTGTTCCCTCTTTGATGCA GAGAGCCTTTCTCTGTAAACACTGGAGAGCAGGGCTTCACTGTCTGGCTCCCCTCCCGCCTCTTGCAGGA AGGACTAGATGCTGCTGATCAGCTGATCATGGCTGAGCCGGGTAGAGCTTGGGGCTCTCCTAAGAGGTCA CCCACTGGGAGGGAGCTTCACAGCTGTTCTTTTGACCCAGGAAGGAGCTCTTTCAGGACTGTGGCACCCA GAGCCGTGGCCCTCCTGCCATGCCATTGTTCCCACTTCCCTGCCCTTGGGAGTCTTGGAGACAGCTGACT AGCTGCCCTGAGGGGTCCACCGCTCTCACCTTCTCACTGGTGGCTGCTGCCATTCTCCTGGGCTCTGCAC AGAAAGCTTTGGTCCTTCTGAAAGCCACTCTGCCCCTTCCTGCAGCACCAGATCCCCCTGAGCCGGTACT GCTGCCTCCACCTGGGCCAAGACTAGTCTTGGTGTTCACCCTTCTTCGTGCTTCAGAAAGTAGGGATATC TGGGGACATGACTGTGTTTCCCTGGGGTCCTTTCAGAAGAGACTTAGGAAGCTCCTGCTCAGGAATCTGG CCCTCTGAAAGAGCCCCTTCAAAGCAGCCTGGGCAGCAGGTAACGTGGTTTCCCTTGCGGCCCCCTCCTC CCAGCTGCATAGACTCGTGGTTTGAAGTGAACAGATCTTGTCCGGAACACCCTGCGGACTGACCTGCGGG CTTGCTTGCTGACTCCTCTCAAAGGGACAGAGCGCCCCTGCTCCAGGGAGGAGGCTCACCGGACCCTGGG GCAGAGCTGAGCTTGGGACACCAGCGGGAACAGGGCACCCCTTCTGCACTGACTTCCAGATCATGGTTCT CCCTTCCTCCCTGAGGACACCAAATTGGATGAGAGCAAGTTTGAGAGAAGAATGAATCAACTGCTATCCT TCCCCTCACCCCTCAGCCCAGGAGGGAAAGGGCATTTTCTTTTTCATCTTTGAAAGGCATTGTGGGTCTG TCTTTAAAGTGTTTAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK094870 unedited GTCAAATATAGNATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTCTGGCTCC CCTCCCGCCTCTTGCAGGAAGGACTAGATGCTGCTGATCAGCTGATCATGGCTGAGCCGG GTAGAGCTTGGGGCTCTCCTAAGAGGTCACCCACTGGGAGGGAGCTTCACAGCTGTTCTT TTGACCCAGGAAGGAGCTCTTTCAGGACTGTGGCACCCAGAGCCGTGGCCCTCCTGCCAT GCCATTGTTCCCACTTCCCTGCCCTTGGGAGTCTTGGAGACAGCTGACTAGCTGCCCTGA GGGGTCCACCGCTCTCACCTTCTCACTGGTGGCTGCTGCCATTCTCCTGGGCTCTGCACA GAAAGCTTTGGTCCTTCTGAAAGCCACTCTGCCCCTTCCTGCAGCACCAGATCCCCCTGA GCCGGTACTGCTGCCTCCACCTGNGCCAAGACTAGTCTTGGTGTTCACCCTTCTTCGTGC TTCAGAAAGTANGGATATCTGGGGACATGACTGTGTTTCCCTGGGGTCCTTTCAGAAGAG ACTTANGAAGCTCCTGCTCANGAATCTGGCCCTCTGAAAGAGCCCTTCAAAGCAGCCTGN GCAGCANGTAACGTGGTTTCCCTTGCGGCCCCCTCCTCCCAGCTGCATAGACTCGTGGTT TGAAGTGAACAGATATTGTGCGCAGCACACTCCTCCTGACTGAACTGCCGGCTTGCCTTG CTGACTCCTCTCAAAGGGACAGAACGCCCCTGCCTCACGGAAGAGGCTCACCGAACCCTG GGGCAAAGCTGAGCTTGGACACCACCCGGGACAGGGCACCCCTTCTGCACTGACTTCCAN ATCATGGTCTCCCCTTCTTCCTTGAAGACACCAAATGNTTGANACCAGTTTCTTGNAGAG ATGNATCAACTGTTATCTTTCCTCCACCCTTANCCAGGAGGGAAAGGCCATTTCTTTTAA TCTTTGAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK094870 unedited GAAAATCCAGGTACCGTGCCGCAATCGAGGATTTAGTTAAAATCTTTTTTTTTTGTAAAC ACTTTAAATACAGACCCACAATGCCTTTCAAAGATTAAAAAGAAAATGCCCTTTCCCTCC TGGGCTGAGGGGTGAGGGGAAGGATAGCAGTTGATTCATTCTTCTCTCAAACTTGCTCTC ATCCAATTTGGTGTCCTCAGGGAGGAAGGGAGAACCATTANCTGGTATTCAGTGCATAAG GGGTGCCCTGTTCCCGCTGGTGTCCCAAGCTCAGCTCTGCCCCAGGGTCCGGTGAGCCTC CTCCCTGGAGCAGGGGCGCTCTGTCCCTTTGAGAGGAGTCAGCAAGCAAGCCCGCAGGTC AGTCCGCAGGGTGTTCCGGACAAGATCTGTTCACTTCAAACCACGAGTCTATGCAGCTGG GAGGAGGGGGCCGCAAGGGAAACCACGTTACCTGCTGCCCANGATGCTTTGAAGGGGCTC TTTCAGAGGGCCAGATTCCTGAGCAGGAGCTTCCTAAGTCTCTTCTGAAAGGACCCCAGG GAAACACAGTCATGTCCCCAGATATCCCTATTTTCTGAAGCACGAAGAAGGGTGAACACC CAGACTAGTCTTGGCCCCAGTGGAGGCAGCAGTACCGGCTCAGGGGGATCTGGTGCTGCA GGAAGGGCCAAAGTGGCTTTCAAAAGGACCAAGCTTTTTGGGCAGAGCCCAGAGAATGGC TGCACCCCCCCTGAAAACGTGAAAACCGTGGACCCCTCAAGGGCGCTTGTCACGTTGTCT CCCAAACTCCCAAGGGCAGGGGATTGGGGAACAATAGGCTTGGGCAGGAGGGGCCACGGC TTCTAGTGGCCCAATTCCTTGAAAGAGCTCCTTCCTGGGGACAAAAACACCTTGGGGAAC CCCCCCCCTTGGGGGGCCTCTTGGAAGACCCCC
Restriction Sites	NotI-NotI
ACCN	AK094870
Insert Size	1000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK094870.1
RefSeq Size	2046 bp
RefSeq ORF	2046 bp
Locus ID	84937
Protein Families	Druggable Genome
Gene Summary	This gene encodes an E3 ubiquitin-protein ligase that plays a role in neural-cell differentiation. Overexpression of this gene causes neurite-like elongation. The encoded protein contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment, indicating that it may be involved in ubiquitin-mediated protein modification, and in synaptic vessicle membranes in neurons. [provided by RefSeq, Feb 2012]

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