GNL3L (AK022325) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GNL3B
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK022325, the custom clone sequence may differ by one or more nucleotides CTGGACCTGGGAGGCAGAGGTTGCAGTGAGCCCAGACGGCACCATTGCACTCTAGCCTGGGCAACAACAC GAAACTCCGTCCCAAAAGAAAAAAAAAAAAAAAATCCCAAGGGGCTGCAGCTGCCAAACCCAATACCCTC TATTTAACCCCTACTCTGTTTTACAAGAGAAATAAAAGAAGTATCAGCAGAGCTCAGGTGCTAACACCTG TTGAGGGCTGACCTACAAAACTCTGCCTACAAAACTCTCTTAGACAGGTGAATATGCCACTAGAAGTTAG GTTGCTGGTAGACCTGGGGGTCCCTGCGGGAGGGTGATGGTTTCTTTACCACCCCACAGGAGATTTCAGT GGCAAGGCATGCCTGCAGTGGGCTTTGGGCCATGCATCTTCCAAGTCCATAGGTCTTCACCTGGGTGGCA GTGAGAAAAAGTAGAAAGTAATGAGCCTCCTGTGTCTCTGGAAGGTTCTAGGGATAGGGTAGAGGGAAGA AGAGAACAAACAAGCCTGGCTTGTGCTGAAGTGTGGTAGGCACTACCCTGTTTGCGTGAAGAGAAAACAA AGCACCTGTTAGTAGGGAGGCTTTAGGGGGAAGCCCCGTCTTGGGGGCATTTCTGGGCAGATTGTGAATT GGAGGAATCTCTTTAACTGAAGTACTCTGGCTGGACCCTGCCCTTGTGTGACCATGTCTCCTATTGCACC AGCATTTGAATTCCATGGCTCAAGAGGGTTCTGGTACCATTTATTCACAGACTGTATCCTCGAGAGAGCT GCTATATATGGGAGTGTACCAGCCAACTCCTTTTCCAGTGTCTGTAAGTCACCTCATTAAAGTATAATTA GCTGTCTCCTCTGGGAGATCCTACCCCATCAGACAAGGGCAGTGAGCCCAAGCAGTGCCAGAGGCCCTCA GAAAGGGATTAGGGTAGATGATTGCAACTGAAACACAATCTTCTTTCTTTGCCAGGGTATTTTGGGGGTT TTGCCCCAAAATATACCCTGGGCATAGCATTACTGCAGTCTTGGATGTCTACCCCAAACTTCCACACCAT CCTTCGACCCACAGCTGCACCTTTATTTATTTATTTTGCTCTGTTGCCCAGGCTGGAGTGCAATGGCGTG ATCTCGGCTCGCTGCAACCTCTGCCTCCCGGGTTCGGGTGATCCTCCTGCCTTAGCCTCCCGGGTAGCTG GGATTGCAGGCACCTGCCACCACGCCTGGCTGGTTTTTGTGTTTTTGGTGGAGACGATGTTTCGCCGTGT TGACCAGGCTGGTCTCGAACTCTTGACCTCAGGTGGTCCGCTCGCTTCGGCCTCCCAAGGTGCTGGGATT ATAGGCGTGAGCCACTGTGCCCAGCCTCACAGCTGCATCTTAACCTTGCCTTTGCCTCTGCCTCTCAAGC TGGTACCTCCTAATTTACATCCTAAGAGTGGAACCATGTGACAAGGACTGGAGTGCCATTGGCTGTGGAC TGTTCAGGCAGGGAAGTACAAGACCACTCTTGTATTCAGGGGCAACCAAAGGAGAGAATTACGTACTTGT TGAGTACAAACTGCACCAAGCCCTGGAGACCCATTACCACCGTTAACCCTCAATACAGCTCTGCTAGTAA GGGATTCGTCTCCCCAAATGAAAAAAAAAGTAATCTCAGAATGTTGGCCGAGCGTGGTGGCTCACACCTG TAATCCCAGTTATGCAGGAGGCTGAGGCAGGAGAATCGCTTGATCTCAGGAGGCGGAGGTTGCAGTGAGT CGACATCACGCCACTGCACTCCAGCCTGGGGGACAGAGTGAGACTTCGTCTCGG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK022325 unedited TCAGATTATGTAATACGACTTACTATAGGGCGGCCGCGAAATTCGCACGAGGCCTCGTGC CGAATTCGGCACGAGGGAGAAAACAAAGCACCTGTTAGTAGGGAGGCTTTAGGGGGAAGC CCCGTCTTGGGGGCATTTCTGGGCAGATTGTGAATTGGAGGAATCTCTTTAACTGAGGTA CTCTGGCTGGACCCTGCCCTTGTGTGACCATGTCTCCTATTGCACCAGCATTTGAATTCC ATGGCTCAAGAGGGTTCTGGTACCATTTATTCACAGACTGTATCCTCGAGAGAGCTGCTA TATATGGGAGTGTACCAGCCAACTCCTTTTCCAGTGTCTGTAAGTCACCTCATTAAAGTA TAATTAGCTGTCTCCTCTGGGAGATCCTACCCCATCAGACAAGGGCAGTGAGCCCAAGCA GTGCCAGAGGCCCTCAGAAAGGGATTAGGGTAGATGATTGCAACTGAAACACAATCTTCT TTCTTTGCCAGGGTATTTTGGGGGTTTTGCCCCAAAATATACCCTGNGCATAGCATTACT GCAGTCTTGGATGTCTACCCCAAACTTCCACACCATCCTTCGACCCACAGCTGCACCTTT ATTTATTTATTTTGCTCTGTTGCCCAAGCTGGAGTGCAATGGCGTGGTCTCGGCTCACTG CAACCTCTGCCTCCCGGTTCANGTGATCCTCCTGCCTTAGCCTCCCCAGTGGCTGGGATT GCNGGCACCTGCCACCACGCCTGGCTGGTTTTTGTGTTTTTAGTGGAGACGGTGTTTCCC CATGTTGGCCGGGCTGGTCTCGAGCTCTTGACCTCGGGTGATCCGTTCGCTTCGGCCTNC CGAGTGCTGGGATATTAGGCGTGGCCACTGTGCCCAGCCTNACAGCTGCATCTTAACCTT ACTTTGCCTCTGCCTCTCAGCTGTACCTNCTAATTACTNCTAGAGTGGACCTGTGACAAG ACTGNATGCATT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK022325 unedited CATCGTTTATCCCCCCATTATCTATTATCGAGTTTTTTTTTTTTTTTTTTCCGANACGAA GTCTCACTCTGTCCCCCAGGCTGGAGTGCAGTGGTGTGATGTCGACTCACTGCAACCTCC GCCTCCTGAGATCAAGTGATTCTCCTGCCTCAGCCTCCTGCATAACTGGGATTACAGGTG TGAGCCACCATGCTCGGCCAACATTCTGATATTACTTTTTTTTTTCATTTGGGGAGACGA ATCCCTTACTAGCAGAGCTGTATTGAGGGTTAACGGTGGTAATGGGTCTCCAGGGCTTGG TGCAGTTTGTACTCAACAAGTACGTAATTCTCTCCTTTGGTTGCCCCTGAATACAAGAGT GGTCTTGTACTTCCCTGCCTGAACAGTCCACAGCCAATGGCACTCCAGTCCTTGTCACAT GGTTCCACTCTTAGGATGTAAATTAGGAGGTACCAGCTTGAGAGGCAAAGGCAAAGGTAA GGTTAAGATGCAGCTGTGAGGCTGGGCACAGTGGCCCACGCCTATAATCCCAGCACTTCG GGAGGCCGAAGCGAGCGGATCACCCGAGGTCAAGAGCTCGAGACCAGCCCGGCCAACATG GCGAAACACCCGTCTCCACTTAAAACACAAAAACCAGCCAGGCGTGGTGGCAGGTGCCCG CAATCCCAGCCACCTGGGAGGGTAAAGCAGGAGGATCACCTGAACCCCGGAGGCAAAAGT TGCAATGAACCCAAAACACCCCTTTGCCTTCAACCTGGGCACCGAGCAAATAAAAAAATA AAGGGGCTCTTTGGGTCAAAAGATGGGGGGAAATTTGGGGGAAAACACCAATAATGCAGA AATGCTTTCCCAGAGTATATTTTGGGGCCCAAACCCCGAAATACCCTGTGAAAAAAAAAA ATATGGTTTATTTGCAACATCTACCCATAACA
Restriction Sites	NotI-NotI
ACCN	AK022325
Insert Size	1400 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK022325.1
RefSeq Size	1804 bp
RefSeq ORF	1804 bp
Locus ID	54552
Protein Families	Protease
Gene Summary	The protein encoded by this gene appears to be a nucleolar GTPase that is essential for ribosomal pre-rRNA processing and cell proliferation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]

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