CCNO (AK026075) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CCNU; CILD29; UDG2
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK026075, the custom clone sequence may differ by one or more nucleotides ATGGTGACCCCCTGTCCCACCAGCCCCTCGAGCCCCGCCGCCCGAGCGGGGAGGCGGGACAACGACCAGA ACCTTCGCGCCCCGGTGAAGAAGAGCAGGCGTCCGCGCCTCCGGAGGAAGCAGCCGCTGCATCCCCTGAA CCCGTGCCCGCTCCCGGGAGACTCCGGCATTTGCGACCTGTTCGAGTCCCCCAGCTCCGGCTCAGACGGC GCAGAGAGCCCCTCTGCGGCGCGGGGTGGTAGCCCCCTGCCCGGCCCGGCCCAGCCCGTGGCGCAGCTAG ATCTACAGACCTTCCGCGACTACGGCCAGAGCTGCTACGCCTTCCGCAAGGCGCAGGAGAGCCACTTCCA CCCGCGGGAGGCGCTGGCACGGCAGCCACAAGTGACGGCGGAATCCCGCTGTAAGCTGCTCAGCTGGCTG ATCCCGGTGCACCGCCAATTCGGCCTCTCCTTCGAGTCGCTGTGCCTGACGGTGAACACTCTGGACCGCT TCCTCACCACCACGCCGGTGGCTGCAGACTGCTTCCAGCTGCTTGGGGTCACCTCCTTGCTCATCGCTTG CAAACAGGTGGAGGTGCACCCGCCGCGCGTGAAGCAGCTTCTGGCCCTCTGCTGCGGCGCCTTCTCCCGG CAGCAGCTCTGCAACCTCGAGTGCATCGTGCTGCACAAGCTGCACTTCACCCTGGGTGCGCCCACCATTA GCTTCTTCCTGGAGCATTTCACGCACGCTCGCGTGGGGGCGGGGCAGGCTGAGGCCTCCGAAGCTCTGGA AGCGCAAGCCCTGGCGCGGGGGGTGGCAGAGCTGAGTCTGGCCGACTATGCCTTCACCAGCTACTCCCCT TCCCTCCTGGCGATCTGCTGCCTGGCGCTGGCGGACCGCATGCTGCGGGTCTCGCGGCCCGTGGACTTGC GACTGGGAGACCACCCGGAGGCGGCGCTGGAGGACTGTATGGGCAAGTTGCAGCTGCTGGTGGCCATAAA CAGTACTTCCTTGACTCACATGCTGCCCGTTCAGATCTGCGAGAAGTGCAGCCTGCCCCCGAGCTCGAAA TAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK026075 unedited TCAAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGATTGGCACGAGGTTCGAGTGC GCGTTTGCTGCCCGCCGCAGCCCGGGCGTTGAAGGTAGTAAAGCGGCCACCCGGCCGCAT CATGGTGACCCCCTGTCCCACCAGCCCCTCGAGCCCCGCCGCCCGAGCGGGGAGGCGGGA CAACGACCAGAACCTTCGCGCCCCGGTGAAGAAGAGCAGGCGTCCGCGCCTCCGGAGGAA GCAGCCGCTGCATCCCCTGAACCCGTGCCCGCTCCCGGGAGACTCCGGCATTTGCGACCT GTTCGAGTCCCCCAGCTCCGGCTCAGACGGCGCAGAGAGCCCCTCTGCGGCGCGGGGTGG TAGCCCCCTGCCCGGCCCGGCCCAGCCCGTGGCGCAGCTAGATCTACAGACCTTCCGCGA CTACGGCCAGAGCTGCTACGCCTTCCGCAAGGCGCAGGAGAGCCACTTCCACCCGCGGGA GGCGCTGGCACGGCAGCCACAAGTGAGGTGCTGGTAGAGCCAGCTCCACGCCGCCGCTCT TCCTCTCCTCCCAGGCTCTCTCCTCTCCTGCCGGCTCGGCCCTCCGAGCGCCCGGGCCCG CGACAGCCCAGGTCCCGCCCCGAGTCGAAGCACAAGGGGCCGTGGAGGGGCGTCACCGGC CTCATTTTACAGATGGAGAAAAGTGGGGCGTAGACGGCTTGAGGGACTTGCCCCGTCCCA AGCTGGCTACTGGGGCACGGTAGAGAGGAATTCGTTTTCGGGTTGAGTCTGGACTCCTAC CGAACTCGNGCAAGTTATTTAACACCCCTGAGCTTCTNTCTTCATCTGCAAATGGAACCC CTCCCCTTNCTTTTGATGCTGAGCCATACGCAGGGTACGTGTCGACGCTCTTGACAGNGC AGGTCATTCCGGGTGTCGCTAGACGGCCAGCGTTTCCAGTCCATGCGTNTCTTCTGAATC TCTGCCCTTCT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK026075 unedited GGTTTTACTATGNACCGCGGCACGCTATCTAAGATCGGTTTTTTTTTTTTTTTTTTAAAC AAACCACAACTGTTTATTGGTGAAAGACTCAGCTTCCTCATGTGCTCGCTGCAAAATAAA TAAAATACCAGATGCTAGTATCGTACACTATTTACAACCTGCAGCTGACCAAGCAGGTCC TGAAGCCTTCTCTGTGGACCAGTACTGCACTCTTCTGAGGCTACGGGAGAGGTCCAGCAG CCGGGCCAGGGACTAAAAGGAAACGAAGGATCTGTTTTATTTCGAGCTCGGGGGCAGGCT GCACTTCTCGCAGATCTGAACGGGCAGCATGTGAGTCAAGGAAGTACTGTTTATGGCCAC CAGCAGCTGCAACTTGCCCATACAGTCCTCCAGCGCCGCCTCCGGGTGGTCTCCCAGTCG CAAGTCCACGGGCCGCGAGACCCGCAGCATGCGGTCCGCCAGCGCCAGGCAGCAGATCGC CAGGAGGGAAGGGGAGTAGCTGGTGAAGGCATAGTCGGCCAGACTCAGCTCTGCCACCCC CCGCGCCAGGGCTTGCGCTTCCAGAGCTTCGGAGGCCTCAGCCTGCCCCGCCTCCACGCG AGCGTGCGTGAAATGCTCCAGGAAGAAGCTAATGGTGGGCGCACCCAGGGTGAAGTGCAG CTTGTGCAGCACGATGCACTCGAGGTTGCAGAGCTGCTGCCGGGAGAAGGCGCCGCAGCA GATGGCCAGAAGCTGCTTCACGCGCGGCGGGTGCACCTCCACCTGTTTGNCAGCGATGAG CAAAGGAGTGACCCCAAGCAGCTGGAAGCAGTCTGCAGCCACCGGCGTGGTGGTGAGGAA ATCGTCCANAGTGTTCACCGTCAGGCACAGCGACTCGAAGGAGAGCCGAATTGGCGT
Restriction Sites	NotI-NotI
ACCN	AK026075
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK026075.1, BAB15351.1
RefSeq Size	1448 bp
RefSeq ORF	1053 bp
Locus ID	10309
Domains	cyclin_C, cyclin
Gene Summary	This gene encodes a member of the cyclin protein family, and the encoded protein is involved in regulation of the cell cycle. Disruption of this gene is associated with primary ciliary dyskinesia-19. Alternative splicing results in multiple transcript variants. This gene, which has a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase in PubMed ID: 2001396. A later publication, PubMed ID: 8419333, identified this gene's product as a cyclin protein family member. The UNG2 symbol is also used as a specific protein isoform name for the UNG gene (GeneID 7374), so confusion exists in the scientific literature and in some databases for these two genes. [provided by RefSeq, Jul 2014]

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