RPS6KA3 (AK095738) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for AK095738 edited GAATTCGGCACGAGGGCAAGACAAGCATCCAGTTTTTTTCTGTAATGCAGTGACAGTGTG GAATGACCACTCAGCCATTATAAATTTGTGTACTTAGGGCCCTTGTCTCACCCAACTATC TCTATAAACTGCTGCAGTAGCTTATTTGTACAAGACACTTGTGAAAATCAGTTTTCTTAC ATTATAGTCATTGTCACATATGGAAGTTGGTCTTTGATTCCAAAAGTAAAGGGAATATGT TTCTACATTAACAGGAATATGGAGAAATCTGTAACGTTTGAATTATCTTTGAAAGAATGG CTAAATGATAAATGTTAAGTCCTGTGCCTATATGGAGAGGACTCTGTACCAGCATGAGTT AATTTAAGTGGATTCACTGCTATTCAGTTCATAGCACATTAGTGACTAGGAATGTGTGTT CAGCCTCCTGAGAGAGGAGGGGAGGAAGCTTGATACTGGGTGGAAACAACTCTTGACATC TGTTTCCTTGTGTGTCAAAGAACGAATTTGTATCAAATAGATCTGTTTTGAGCTTTGGCC ATTATTTGGCTAAGAACTTCAGCCATCTCATCTCAGACAAATTGGACAGCCTCTTTGATA CATTTTCTAGTAGTCAAAATGGGGCAGATGTAGTTTAATATGGGTGGAAAACTGCCCCAG CAAGATACCAGGTTATTTACTGTGGTATCGAAAGCTCTAAGAGAATGGAAGTGGTTCTTT TGCCCGGCTGGAAATAGCAGTGGGAAGACAAGTCTATCCAGACCAAGCCTGTCCATACAA CACTGGCAATCAGTTGATGAGGGAATTAAGTCAGATGGGAGACGGCTTTTTTTXXXXXXX XXXXXXXXXXXTCATATTGTTAGCCAAACGCTGATTAAAATGCAAATTTCAAACTAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK095738 unedited TCAAAATTTGTAATACGACTCACTATAGGGCGGCCGCGNAATTCGCACGAGGGCAAGACA AGCATCCAGTTTTTTTCTGTAATGCAGTGACAGTGTGGAATGACCACTCAGCCATTATAA ATTTGTGTACTTAGGGCCCTTGTCTCACCCAACTATCTCTATAAACTGCTGCAGTAGCTT ATTTGTACAAGACACTTGTGAAAATCAGTTTTCTTACATTATAGTCATTGTCACATATGG AAGTTGGTCTTTGATTCCAAAAGTAAAGGGAATATGTTTCTACATTAACAGGAATATGGA GAAATCTGTAACGTTTGAATTATCTTTGAAAGAATGGCTAAATGATAAATGTTAAGTCCT GTGCCTATATGGAGAGGACTCTGTACCAGCATGAGTTAATTTAAGTGGATTCACTGCTAT TCAGTTCATAGCACATTAGTGACTAGGAATGTGTGTTCAGCCTCCTGAGAGAGGAGGGGA GGAAGCTTGATACTGGGTGGAAACAACTCTTGACATCTGTTTCCTTGTGTGTCAAAGAAC GAATTTGTATCAAATAGATCTGTTTTGAGCTTTGGCCATTATTTGGCTAAGAACTTCAGC CATCTCATCTCAGACAAATTGGACAGCCTCTTTGATACATTTTCTAGTAGTCAAAATGGG GCAGATGTAGTTTAATATGGGTGGAAAACTGCCCCAGCAAGATACCAGGTTATTTACTGT GGTATCGAAAGCTCTAAGAAATGGAAGTGGTTCTTTTGCCCGGCTGGAAATAGCAGTGGG AAGACAAGTCTATCCAGACCAAGCCTGTCCATACAACACTGGGCATCAGTTGATGAGGGA ATTAAGTCAGATGGGAGAACGGCTTTNTTTTTTTTTTTTTGAGNTGGAGTCTCATCTGTC CCCAGNNTGAGTGCANTGGTGGNGACTCAGCTACTGCAACCTCACTTCAGGTCAGCATNT CTCTGCTCAGCTTANACACTGGGT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK095738 unedited GGTCTATCTATGNACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTAGTTTGAAGAGGGGGATTTTAATCAGCGTTTGGCTAACA ATATGAGATAAACTATTAAAGAAGTGGAGGGCATTCTGGCAAATAAAAAGTTCCCTCATT TCACACTTAAAAAGCTGCCACGAAAAGCTTATTCTTGGAGTATAAAATGCAAAATTTAGG TTAAACCCGGCGGGGGGGAGATGAAATTGGACCCTGGCAATTTCTTATGGGTCAACCTAA TAGTAAAACATTTATAAACAATTGGATCATTACAAATACCTTTAGACATCGTTTTCTGCT AAACTCAGTATTTTCCTTTCCATCCTGATCAAAGAAACATTATTGCAGTAAGCCACATTA TAATTCAGCACGATTTCTTCTAAAAAGGCTCCGAGTTCCTTTGAGCTTGTTATTGTACAG TGGATTTTTCATTTGCAAAGACGTTAAGCCCTCCAAATGTGCAAATCATGAAGTCAGTTG TTGTTCCAGCAAGGGTTGCCCAGCGGTAAAAAACAAGATAAACTAATGCACTAGCTGAAA CCAGGTGGGGAGACCATGTGTGGTAATGCTTGGGGGTGGAAGGAAAACTATTTCTGAATG AGGACTTAAAGTTAATACCAGCTTCACTGGCTGGTCACATGAGAAACCCAAAGCTCCATT TTAACTGCAGCATTAGGAGGTTCACAACTGGCAACCCAAACTGGTTAAATCCCAAACTAG GTTCTAAAAGGGTAGGATCAAACCAAAAATTCGGGAGGGATCTCAGTAACTTTCAAATTG GCCGACCCCCGAATTCCCCTTTAAAAATTAGGCCCAATTTTTTAACCTCTTTCCTAGGGG ATTGGCTTTTTGG
Restriction Sites	NotI-NotI
ACCN	AK095738
Insert Size	2750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK095738.1
RefSeq Size	2410 bp
RefSeq ORF	2410 bp
Locus ID	6197
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Long-term potentiation, MAPK signaling pathway, mTOR signaling pathway, Neurotrophin signaling pathway, Oocyte meiosis, Progesterone-mediated oocyte maturation
Gene Summary	This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008]

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