PARP9 (AK092629) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARTD9; BAL; BAL1; MGC:7868
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK092629, the custom clone sequence may differ by one or more nucleotides GCTTTTTCAGCCGTGCAATATTCCAAATTATAGGACTCTCAGTCAATTTAGATGGCCACCCACAGGCAAA GGAGACAGAACTTCTGTTAACAAACCTTTATTTTCTTTTTTCTATTTATGTTTGCATTCCAACCAGTTCT GAATAAATCTCTTGCTTGTAGGACTTGGTTGAGTGCAGCAAGAAGGCATCTACACATGCAGTTTCTTACT GGGTTGATTCATTTGTTCCACAGGTATTTATTAAGCACATTCTATGAATCAGGCCCTGTGCTAGGGGTAC TAGAAATAGAGTGTAAATAAAAAACCATAGAGTTCTTGCCCTTACAATTTATAATCTCACGAGTTCACCT GTTGCCCATTCTTTATCTTCTCTCTTTTTTCCTCTACGATGTCCCCCTTGGTGATGGCACCTGTTCCCAT GGCCTCAACTCCTACCTCAATGATGACGACTTAGGAATTTCTAACTCTTAACTCCCTACCTCTCTCCTGA CCTGCAGGTTCATATGTCTCACTCTCTTTTTGACATCTTTACCCAGATGTCCCATAGGTAACTCAAATTG ATCCCTTTTCTTGGTCATGATTTTCCCTAAGAAATATGCCCTTTGTTTGCTGATTCTCCCTAACCTGGTT AGTATCATCACCATCTACCTACCAGGTTTCCTAAGGCTGAAACATTATCATCCTCCATTCCTCCTTTTCC CTCACCCTTATACCCAGGCATTCACCCAGTCCTAGTGATTCACTCTCATGAAGATCTCAGCCCAGTTTCA CCTCTCTATTCCTACTGCCATAGTTAATCTTCCTTCAGACCTAGACTATTACTAGGTCCTCTCCTTGCCA CAAAACACACCCCTACCTAATCTATCCTTTACCACCAGAATTATCACGCTAAAAATTACATTCTTGCTTA ATATCTGTATTCCCAGTTGCCTACGGGATAAAAGCCCAAACTCCTTAGCAGAGAATATAAGGCCCTAGCT CCCACATTATTTCAGCAGTCATCACCCACTATGTTCCTCAAGACTGCAGCCATTAACTTTTTAGAGTTCC CTAAACATGCTGTTTACTTTCATGCCTCTATCCCGTTGTCTGTGGAATGACTTCCCTCCTTGCCCTTTTC AGTGCTACAAACCCCTATTCTTTAAGACATAGTACAAATGGCATCTCCTGGTTGGCATCTTTCCTGCAGG CCTACAGGCCTAGTAAGTATCTTCCTCCTCTGTGCTCCTGCATACCTCCATTCCTTTGTTATGACATCTA TAACTTTAATAAGTACTAAAATCTGTAGTCCTACAAAACTCAGGCATAGAACTCATTTCCTTTATGGCTC TATAATGGAACTTTACCCAACTCTCACGTTCCCCATGACCACAGATGTGGAAAATTTGAATCTTGACAGT TCAAGGTGAACTCAGTCATTTTCAGAGTTTTCATAGTCCCTTCAAGATTGAAACTCAGTTCCTGCAATGT TTGCCCCTTTTCTCCTCTTTTGTCTATGCTGGGAGAGGCATTGTGGGGAGGGTTGTCTGGCTTATGGCTC CCATTGTCCTCTGCTTGATAAACCACCTGAGCTTTGGTCATTAGCAGTCTCCTGTGCCTTTCACACTCAG GTAGTGTCTGCACAGGCCACTCTATGTCTTTTCCATGCTGAAGAAATTCCTTTCCAGGCCATGTCTGTGT TCCTCCTGCCACACAGGAAATTTTTGAGCATGTTCATCCTCCAAGCTGAATGCAGGGTCTTGGGTAGTGG TCCTCACCTGCTCCAGAGACTTCTCCAGCCATTGCCACTCTCCACTCAGGTGATGAAGCTGGATGAGGGA CTGCACCCACCAGAGTCAGGCCAGGGTCCTGTCTGCTCTGTGAGTCCCTCCAATTGTTCTTATTCCGAGA TTTCCATTGTTCTGCCCCCTCTTGACTCCCAGGGCTCTCAAGGGAGTGGGGGTAGTGAAGGGAGCCCTTT CCCAAGCTCCCCCAAGAGCTCTAGTCACATCACTTCTGATACTTCTTTTCCCACCAGCTGGAAGAAAGAA CTTTCATTTGTCTTGAAATGAGAAAAATGTTCTTAGAATATTTTGTATTACTCTCTGCTCTGTCATTTAT GGTAAACAAAATAAAATAATAAAAAAAAAC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK092629 unedited GCGTTGCAGNATTTTGTATACGATTCACTATAGGGCGGCCGCGAATTCGCACGAGGGCTG TCTGTGTGCTCTGCCCTTCTCCCTATCCCTTAGCTTAACAGCCACTGCCTTGAGGCAATT CAGACATGACTCATAATGTGACCTGCACCAATTGACTCATCTCTGTAGGCTAGAGAGAAC TCTTAACAGAAGGTGCCAGACAAGCCCAGGAGACACCAATCTTGCTACCACTGAAGCTGT TGTTATTTGGAGTACAAAAGCTCTTAGCTTTTTCAGCCGTGCAATATTCCAAATTATAGG ACTCTCAGTCAATTTAGATGGCCACCCACAGGCAAAGGAGACAGAACTTCTGTTAACAAA CCTTTATTTTCTTTTTTCTATTTATGTTTGCATTCCAACCAGTTCTGAATAAATCTCTTG CTTGTAGGACTTGGTTGAGTGCAGCAAGAAGGCATCTACACATGCAGTTTCTTACTGGGT TGATTCATTTGTTCCACAGGTATTTATTAAGCACATTCTATGAATCAGGCCCTGTGCTAG GGGTACTAGAAATAGAGTGTAAATAAAAAACCATAGAGTTCTTGCCCTTACAATTTATAA TCTCACGAGTTCACCTGTTGCCCATTCTTTATCTTCTCTCCTTTTTCCTCTACGATGTCC CCCTTGGTGATGGCACCTGTTCCCATGGNCTCAACTNCTACCTCAATGATGATGACTTAN GAATTTCTAACTCTTAACTCCCTACCTCTCTTCTGACCTGCAGGTTCATATGTCTCACTC TCTTTTTGACATCTTTACCCAGATGTCCCATAAGTACCCTCAAATTGATCCCTTTCCTTG GNCATGAATTTCCCTAAGAAATATGCCCCTTTGTTTGCTGATTCTTCCTAAACTGGGTTG GATCATCACCATTTACCTACCAGTTTTCTTAG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK092629 unedited CTATGGACCGCGGCCGCAATCTAAGATCGAGTTTTTTTTTTTTTTTTTTGGGTTGCTTTT GTTTTATTTTTGTATTTTGGTAACCATAAATGACAAAGCAGAGAGGGATACATAATATTC TAAGAACATTTTTTTCATTTCAAGACAAATGAAAGTTCTTTCTTCCAGCTGGTGGTGAAA AGAAGTATCATAAGTGATGTGACTAGAGCTCTTGGGGGTAGCTTGGGAAAGGGCTCCCTT CACTACCGCCACTCCCTTGGCAGCCCTGGGAGTCAAGATGGGGCATAACAATGGAAATCT CGGAATAAGAACAATTGGAGGGACTCACTGTATCACACTGGACCCTGTCCTGACTCTGGT GGGTGCAGTCACTAATCCCGGTTCATCACTCTGACTGGAGAGTGGCAATGGCTGGAGAAG TCTCTGTGCCAGGTGAAGACCACTGCCCCGGACCCTGCCTTCAGCTTGCAGGATGAACAT GCTCCAAAATTTACTGTGTGGCCGTGAGGAACACAGACATGGCCTGGAAAGGGAACTTCT TCCTCATGGAGAAGACATATTGTGGCCGGTGCAGACACTACCTGAGTGTAAAAGGCACAA GATACTGCTTGCGACCACGCGCTAAGTGGTTTTATCAAGCCAAAGGAGATCGGGTGTCCA TAAGCGCGACCAACCCTCCCCACAATTGCCTTGTCCGGGTCTCTCCTAACTAGGGAGCAT ATACGGGGACAATACTGCCGCGGACTTGCGTTCAAATCCTTGTGGGACTATTGANACCTC TGCATAATGTATGGGAGTTGCTTTGGACCTGTTGCGGATCCACGATTCGACAACTCCTTT AGGTCATAGGGTAGAACGCATATTTCGTTCCAAGTTCCCTCACCGAAGCCCTCATAGAGA TTGCGCTCTGTTTCTCACCTTTGTAGAGATTCCCGCTTCTAGCTTTGTGAAGGTTTTGGA TTGCCAGCCCATGGTTGCGGTTCCGGGAGCCTCTAGAATAACCAATTC
Restriction Sites	NotI-NotI
ACCN	AK092629
Insert Size	2500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK092629.1
RefSeq Size	2130 bp
RefSeq ORF	2130 bp
Locus ID	83666
Gene Summary	ADP-ribosyltransferase which, in association with E3 ligase DTX3L, plays a role in DNA damage repair and in immune responses including interferon-mediated antiviral defenses (PubMed:16809771, PubMed:23230272, PubMed:26479788, PubMed:27796300). Within the complex, enhances DTX3L E3 ligase activity which is further enhanced by PARP9 binding to poly(ADP-ribose) (PubMed:28525742). In association with DTX3L and in presence of E1 and E2 enzymes, mediates NAD(+)-dependent mono-ADP-ribosylation of ubiquitin which prevents ubiquitin conjugation to substrates such as histones (PubMed:28525742). During DNA repair, PARP1 recruits PARP9/BAL1-DTX3L complex to DNA damage sites via PARP9 binding to ribosylated PARP1 (PubMed:23230272). Subsequent PARP1-dependent PARP9/BAL1-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites (PubMed:23230272, PubMed:28525742). In response to DNA damage, PARP9-DTX3L complex is required for efficient non-homologous end joining (NHEJ); the complex function is negatively modulated by PARP9 activity (PubMed:28525742). Dispensable for B-cell receptor (BCR) assembly through V(D)J recombination and class switch recombination (CSR) (By similarity). In macrophages, positively regulates pro-inflammatory cytokines production in response to IFNG stimulation by suppressing PARP14-mediated STAT1 ADP-ribosylation and thus promoting STAT1 phosphorylation (PubMed:27796300). Also suppresses PARP14-mediated STAT6 ADP-ribosylation (PubMed:27796300).[UniProtKB/Swiss-Prot Function]

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