ZNF514 (NM_032788) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_032788, the custom clone sequence may differ by one or more nucleotides ATGACATTTGAAGATGTGGCTGTGGAATTCAGCCAGTGGGAGTGGGGGCAGCTGAACCCTGCTCAGAAGG ACCTCTACAGGGAGGTGATGCTGGAGAACTTCAGGAACTTGGCCATTCTGGGCCTTCTAGTATCCAAACC ATATGTGATCTGCCAGTTGGAGGAAGGGGGTGAGCCCTTCATGGTGGAGAGAGAAATCTCAACAGGAGCC CACTCAGACTGGAAGAGAAGGTCTAAATCCAAGGAATCAATGCCAAGTTGGGGAATTTCCAAAGAAGAAT TATTCCAGGTAGTATCAGTGGAAAAACACATTCAAGATGTGCTGCAGTTCTCGAAGTTGAAAGCAGCCTG CGGTTGTGATGGCCAGTTAGAGATGCAGCAGATAAAACAGGAGAGACACCTGAAACAAATGTCAACCATT CACAAATCTGCCACCACCCTTAGCAGAGATTATAAATGGAATGGATTTGGGAGAAGCTTAGGTTTAAGAT CAGTCCTTGTTAACCAACACAGCATTCTCATGGGAGAAGGATCTTATAAATGTGATACAGAATTCAGGCA GACTTTAGGGGGAAACAACTCTCAGAGAACCCACCCAGAAAAGAAATCTTGTAAATGTAATGAGTGTGGG AAGTCCTTTCACTTCCAGTCAGAACTTAGGCGCCATCAGCGATGTCACACTGGAGAAAAGCCGTATGAAT GCAGTGACTGTGGAAGAGCCTTTGGTCATATTTCATCCCTTATTAAACATCAAAGAACTCATACTGGAGA AAAGCCCTATGAATGCAGTGAATGTGGGAGAGCCTTCAGCCAGAGTTCGTCTCTTGTTCTGCACTATAGA TTTCACACTGGAGAGAAACCCTACAAATGTAATGAATGTGGACGAGCCTTTGGTCACACTTCATCCCTTA TTAAGCATCAGAGGACTCATACTGGAGAAAAGCCCTATGAATGCCGGGAATGTGGGAGAACCTTCAGCCA GAGCTCATCACTCATTGTGCATTACAGATTTCATACTGGAGAGAAACCTTACAAATGTAATAAATGTGGG AGAGCCTTCAGCCAGAGTTCATCTCTCACTCAACATTACAGATTTCACACTGGAGAGAAACCCTACAAAT GTAATGAGTGTGGAAGGGCCTTTGCTCATACTGCATCCCTTATTAAACATCAGAGAAGTCATGCTGGAAA AAAAACCCTATAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_032788 unedited NGGGGTGCACCATTTTGTAATACGACTCACTATTAGGGCGGCCGCGAATTCGCACGAGGC GAATCCCTGCTGTTTCCGGGCAGCTGAAGAGCGCTGGGCCCTCGCGTCGCGGGCGTGGCT GTGGCCGTGTCTCCTGGTAGTCTGAGCCCACTGTGCGTGTGGATCCACGTGGGAGCTGGG TTCCAGAGCCTGGTCCTGAGGAGGAGCCGAGCCGGGGCTTCCCCTTCTCAGAATCCTGCT CTTCCTCCAGAGAGATTCCCAGGAGAAGAGGGAACAACCAATTCATTCCTGAAAGCCAGG CCTCGGGACCTGATGACATTTGAAGATGTGGCTGTGGAATTCAGCCAGTGGGAGTGGGGG CAGCTGAACCCTGCTCAGAAGGACCTCTACAGGGAGGTGATGCTGGAGAACTTCAGGAAC TTGGCCATTCTGGGCCTTCTAGTATCCAAACCATATGTGATCTGCCAGTTGGAGGAAGGG GGTGAGCCCTTCATGGTGGAGAGAGAAATCTCAACAGGAGCCCACTCAGACTGGAAGAGA AGGTCTAAATCCAAGGAATCAATGCCAAGTTGGGGAATTTCCAAAGAAGAATTATTCCAG GTAGTATCAGTGGAAAAACACATTCAAGATGTGCTGCAGTTCTCGAAGTTGAAAGCAGCC TGCGGTTGTGATGGCCAGTTAGAGATGCAGCAGATAAAACAGGAGAGACACCTGANACAN ATGTCAACCATTCACAAATCTGCCACCACCCTTAGCAGAGATTATAAATGGAATGGATTT GGGAGAAGCTTAGTTTAAGATCAGTCCTTGNTAACCAACACAGCATTCTCATGGGAGAAG GATCTTATNAATGTGATACAGNATTCAGGCAGACTTTAGGGGGAAAACACTCTCAGAGAA CCCACCCAGAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_032788 unedited CGCGGCCGCAATCTANATCGAGTTTTTTTTTTTTTTTTTTCTGAGACGGAGTCTCACTCT GTCTCCCAGCTGGAGTGCAGTGGCGTGATCTCGGCTGACTGCAAGCTCTGCCTCCCCGAT CCACGCCATTCTCCTGTCTCAGCCCTCCAAGTAGCTGGGACCACAGGCGCCCGCCACCAT GCCTGGCTAATTTTTTTGTATTTTTAGGAGAGACAGGGTTTCACCGTGCTAGCCAGGATG GTCTCGAACTCCTGACCTCGTGATCTGTCCGCCTCGGCCTCCCAAAGTGCTGAGGTCACA GGCGTGAGCCACCGCGCCCAGCCTATTTTAGTAATTTTAATGTTTAAACAAGGTTTCATT TCATTTCAAAAATTCCAAATCTATTAGCATAAAGATGTAACAAAAATTGCTTTTTGCTCA ATCCTAGACCACTCCATTGCCTCACAAAGAAAGGTAACATGTGCTACAGAGAAAGAAGTG CATAGAGGTGTTGTACTGCAAAGATCCAGTCTATTAATCTCCATACTACAGCTGTGAACC ACAGGAAAGAGGCCCAGCCCAGTTAGGACTATAGGCCAAGGCATGTCACTTTCTGAACAT CAGTTNTATTAGCTCTAAAATACGACTGGGCTAGATCATGGGTAGCTGTCTACCTTTCTC AGCCTNCATCCTCTGCACCCATGCATGACACCCTGCCCCGTCAGTAACAGCATGAGGAGG GCATGCACAAGTGTCNGTATACAAATGGCCCCACCAGCAGAAAATCACCAGACAGGTGAT TTCTCAGCTTCTTTCAGCTCTAGTTTATACATCTCAGTGGGAAAGAAACTTCCTACATTA TCATGTTCCCAGTTTCTCTTGAGAGAAACTGAAGCTGACTTACTATGGGTTNCCAGTTCA CTAAATTGAAAGTGGTCTACCCAATTCTCTAGTACAATTCTTACGTGTTTGTGGAAATCC ACAAGCCTTCCAATTATACGTACCGAAGTCCGCTAA
Restriction Sites	NotI-NotI
ACCN	NM_032788
Insert Size	3290 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_032788.1, NP_116177.1
RefSeq Size	3204 bp
RefSeq ORF	1203 bp
Locus ID	84874
UniProt ID	Q96K75
Domains	KRAB, zf-C2H2
Protein Families	Transcription Factors
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate splice site in the 5' coding region that results in use of a downstream start codon compared to variant 1. It encodes isoform 2, which has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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