EHD1 (AK098723) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	H-PAST; HPAST1; PAST; PAST1
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK098723, the custom clone sequence may differ by one or more nucleotides AGCATTCGGCAGAGGGCGCTTCGACGGGCTGGGCTGTGCGCCTGCGCAGTGTGGGTCGCTCCCGATTCCC TGCCCCGGCCGGCCCCGCCTCGGCTCCGCACCCTCGCCCCGCTCTCAGCCGCCGCTCTGCCCCGCAGCAG CCAGCCCCGTGTCCGGCAGTATGTTCAGCTGGGTCAGCAAGGATGCCCGCCGCAAGAAGGAGCCGGAGCT CTTCCAGACGGTGGCCGAGGGGCTGCGGCAGCTGTACGCGCAGAAGCTGCTACCCCTGGAGGAGCACTAC CGCTTCCACGAGTTCCACTCGCCCGCGCTGGAGGACGCTGACTTCGACAACAAGCCTATGGTGCTCCTCG TGGGGCAGTACAGCACGGGCAAGACCACCTTCATCCGACACCTGATCGAGCAGGACCTCTTCAAGGACAT CCAGTCACTGCCCCGAAACGCCGCCCTCAGGAAGCTCAATGACCTGATCAAGCGGGCACGGCTGGCCAAG GTTCACGCCTACATCATCAGCTCCCTCAAGAAAGAGATGCCCAATGTCTTTGGTAAAGAGAGCAAAAAGA AAGAGCTGGTGAACAACCTGGGAGAGATCTACCAGAAGATTGAGCGCGAGCACCAGATCTCCCCTGGGGA CTTCCCGAGCCTCCGCAAGATGCAGGAACTCCTGCAGACCCAGGACTTCAGCAAGTTCCAGGCGCTGAAG CCCAAGCTGCTGGACACGGTGGATGACATGCTGGCCAACGACATCGCGCGGCTGATGGTGATGGTGCGGC AGGAGGAGTCCCTGATGCCTTCCCAGGTGGTCAAGGGCGGCGCCTTTGACGGCACCATGAACGGGCCGTT CGGGCACGGCTACGGCGAGATCTTCTACACGCTGTCCCCTGTCAACGGCAAGATCACGGGCGCCAACGCC AAGAAGGAGATGGTGAAGTCCAAGCTCCCCAACACCGTGCTAGGGAAGATCTGGAAGCTGGCCGACGTGG ACAAGGACGGGCTGCTGGACGACGAGGAGTTCGCGCTGGCCAACCACCTCATCAAGGTCAAGCTGGAGGG CCACGAGCTGCCCGCCGACCTGCCCCCGCACCTGGTGCCGCCCTCCAAGCGCAGACATGAGTGATGGCGC CCGGCCCCGCACCTGCCATTTGCACGCCCGGCCGGGAGGCAGAGACGGGGGGAGGGGAAGCCTCACCATT TCTCAAGGTCCATAAAGACTGAGCGGATGTTTCCTCGCCTCTCGAAAAGGAAAACCACCATCTTTCTTTT AAGGCTGTTCCTGGGCCTGGCGGGGGAGGCAGGGGTGAGAGGATGGAATTGTGTGCACAAGAACTGTGGC TATTTTAATAT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK098723 unedited TTTTTACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATA AGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCC GCGAATTCGGCACGAGGGGGCTGTGCGCCTGCGCAGTGTGGGTCGCTCCCGATTCCCTGC CCCGGCCGGCCCCGCCTCGGCTCCGCACCCTCGCCCCGCTCTCAGCCGCCGCTCTGCCCC GCAGCAGCCAGCCCCGTGTCCGGCAGTATGTTCAGCTGGGTCAGCAAGGATGCCCGCCGC AAGAAGGAGCCGGAGCTCTTCCAGACGGTGGCCGAGGGGCTGCGGCAGCTGTACGCGCAG AAGCTGCTACCCCTGGAGGAGCACTACCGCTTCCACGAGTTCCACTCGCCCGCGCTGGAG GACGCTGACTTCGACAACAAGCCTATGGTGCTCCTCGTGGGGCAGTACAGCACGGGCAAG ACCACCTTCATCCGACACCTGATCGAGCAGGACTTCCCGGNGATGCGCATCGGGCCCCGA GCCCAACACCGACTCCTTCATCGCCGTCATGCACGGTCCCACTGAAGGCGCGTCCCTCTG TACTCCCCACACACCTCCATTCTCCCCTCATCTCTCCCACTATCCTCTTCCCCCACCCCA CACCCCCCCTCTCCTCCCCCCCCTCCCCACCCCCTCAAACCCCTCTTCCCTCCTCTCTTC CCCACCTCTCTACCCCTTCCTTTTTCTCCTCCTCTCTTTCTTCCACATCCTTCACCCTCT CCCGCTATCCCACTCCTCCTCCCTCTTCCCCCCTTCTCCTTCACCCTTCTCCTCTTCCCC TCTAAACTCACCTCCCCCCACCTCAAACTCCCCACCTATCTACACCCCCCATATCTCCTA CTTCTCCTCTTTATTTTAATTATTATCTCTCTTAATAATTATTACTTTCTTATT
Restriction Sites	NotI-NotI
ACCN	AK098723
Insert Size	3600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AK098723.1
RefSeq Size	1341 bp
RefSeq ORF	1341 bp
Locus ID	10938
Domains	EH, EFh
Protein Families	Druggable Genome
Protein Pathways	Endocytosis
Gene Summary	This gene belongs to a highly conserved gene family encoding EPS15 homology (EH) domain-containing proteins. The protein-binding EH domain was first noted in EPS15, a substrate for the epidermal growth factor receptor. The EH domain has been shown to be an important motif in proteins involved in protein-protein interactions and in intracellular sorting. The protein encoded by this gene is thought to play a role in the endocytosis of IGF1 receptors. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]

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