PIGG (BD127295) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BD127295, the custom clone sequence may differ by one or more nucleotides GACGATAAGGCCTGGCGTTATTGCTTAGAGGCGGCTACCTGGAGCCGGAAGCGCGGCTGCAGCAGGGCGA GGCTCCAGGTGGGGTCGGTTCCGCATCCAGCCTAGCGTGTCCACGATGCGGCTGGGCTCCGGGACTTTCG CTACCTGTTGCGTAGCGATCGAGGTGCTAGGGATCGCGGTCTTCCTTCGGGGATTCTTCCCGGCTCCCGT TCGTTCCTCTGCCAGAGCGGAACACGGAGCGGAGCCCCCAGCGCCCGAACCCTCGGCTGGAGCCAGTTCT AACTGGACCACGCTGCCACCACCTCTCTTCAGTAAAGTTGTTATTGTTCTGATAGATGCCTTGAGAGATG ATTTTGTGTTTGGGTCAAAGGGTGTGAAATTTATGCCCTACACAACTTACCTTGTGGAAAAAGGAGCATC TCACAGTTTTGTGGCTGAAGCAAAGCCACCTACAGTTACTATGCCTCGAATCAAGGCATTGATGACGGGG AGCCTTCCTGGCTTTGTCGACGTCATCAGGAACCTCAATTCTCCTGCACTGCTGGAAGACAGTGTGATAA GACAAGCAAAAGCAGCTGGAAAAAGAATAGTCTTTTATGGAGATGAAACCTGGGTTAAATTATTCCCAAA GCATTTTGTGGAATATGATGGAACAACCTCATTTTTCGTGTCAGATTACACAGAGGTGGATAATAATGTC ACGAGGCATTTGGATAAAGTATTAAAAAGAGGAGATTGGGACATATTAATCCTCCACTACCTGGGGCTGG ACCACATTGGCCACATTTCAGGGCCCAACAGCCCCCTGATTGGGCAGAAGCTGAGCGAGATGGACAGCGT GCTGATGAAGATCCACACCTCACTGCAGTCGAAGGAGAGAGAGACGCCTTTACCCAATTTGCTGGTTCTT TGTGGTGACCATGGCATGTCTGAAACAGGAAGTCACGGGGCCTCCTCCACCGAGGAGGTGAATACACCTC TGATTTTAATCAGTTCTGCGTTTGAAAGGAAACCCGGTGATATCCGACATCCAAAGCACGTCCAACAGAC GGATGTGGCTGCGACACTGGCGATAGCACTTGGCTTACCGATTCCAAAAGACAGTGTAGGGAGCCTCCTA TTCCCAGTTGTGGAAGGAAGACCAATGAGAGAGCAGTTGAGATTTTTACATTTGAATACAGTGCAGCTTA GTAAACTGTTGCAAGAGAATGTGCCGTCATATGAAAAAGATCCTGGGTTTGAGCAGTTCAAAATGTCAGA AAGATTGCATGGGAACTGGATCAGACTGTACTTGGAGGAAAAGCATTCAGAAGTCCTATTCAACCTGGGC TCCAAGGTTCTCAGGCAGTACCTGGATGCTCTGAAGACGCTGAGCTTGTCCCTGAGTGCACAAGTGGCCC AGTACGACATCTATTCGATGATGGTTCTCACCCTGCTCCTGCTCAGCGTCCCACAGGCACTGCGCAGAAA GGCTGAGCTGGAAGTCCCACTGTCATCTCCTGGGTTTTCTCTGCTCTTTTATTTGGTGATCCTGGTTCTT TCGGCCGTTCACGTCATTGTGTGCACCTCAGCTGAAAGTTCGTGCTACTTCTGTGGCCTCTCGTGGCTGG CGGCAGGTGGGGTGATGGTGCTGGCCTCGGCGCTGCTGTGTGTGATTGTGTCTGTTCTGACCAACGTGCT CGTGGGTGGAAACACCCCAAGGAAGAACCCCATGCATCCCAGCTCAAGGTGGTCAGAGCTAGACCTTCTT ATTCTGTTGGGGACGGCGGGCCACGTCTTGAGCCTGGGCGCCAGCAGCTTCGTGGAGGAGGAGCACCAGA CCTGGTACTTCCTTGTGAACACCCTGTGTCTAGCTCTGAGCCAAGAAACCTACAGAAACTACTTTCTGGG AGATGACGGTGAGCCTCCGTGTGGCCTCTGTGTGGAACAAGGGCATGACGGGGCCACAGCAGCGTGGCAG GGCGGGCCTGGCTGTGATGTCCTGGAGCGAGACAAAGGCCACGGAAGCCCCTCTACCTCCGAAGTGCTCA GAGGCCGCGAGAAGTGGATGGTGCTGGCCAGTCCGTGGCTAATACTGGCCTGCTGCCGGCTGCTGCGCTC CCTAAACCAGACAGGTGTGCAGTGGGCTCACCGGCCTGACCTCGGCCACTGGCTCACCAGCTCTGACCAC AAAGCCGAGCTCTCTGTCCTGGCTGCCCTCTCCCTCCTCGTAGTTTTTGTGCTGGTGCAGAGGGGGTGCT CCCCTGTGTCCAAGGCTGCCCTGGCGCTGGGGCTGCTGGGCGTCTACTGCTACCGGGCGGCCATCGGGAG TGTCCGGTTCCCGTGGCGGCCGGACAGCAAGGACATTTCCAAGGGTATTATTGAAGCTCGTTTTGTTTAT GTCTTTGTCCTTGGCATTCTGTTCACGGGCACCAAAGACTTACTTAAATCTCAAGTCATTGCTGCAGACT TCAAACTCAAGACTGTAGGTTTATGGGAGATATATAGTGGATTAGTTCTTCTGGCAGCCTTGCTCTTTAG ACCACATAATCTTCCGGTCTTAGCATTTAGCCTCTTGATTCAGACTCTAATGACTAAATTCATCTGGAAG CCCCTGAGACACGATGCAGCTGAGATTACTGTGATGCATTATTGGTTTGGTCAAGCATTCTTCTATTTTC AGGGCAACTCCAACAACATTGCCACCGTGGACATCTCCGCAGGCTTCGTGGGCTTAGACACCTACGTGGA AATCCCAGCCGTGCTCCTGACAGCGCTTGGGACGTACGCAGGGCCTGTGCTGTGGGCCAGCCACTTAGTG CACTTCCTGAGCTCAGAAACACGCAGTGGTTCAGCACTGAGTCATGCTTGCTTCTGCTACGCACTGATTT GTTCTATTCCAGTTTTCACGTACATCGTTTTGGTGACATCTCTGCGTTATCATTTATTTATATGGAGTGT ATTTTCTCCAAAACTTCTCTACGAGGGAATGCACCTGCTCATTACAGCTGCTGTCTGTGTATTCTTCACG GCAATGGATCAAACCAGACTCACACAGTCTTAGACTAAGCTGAACACTGGAAAAATAATACATGCTTAAA GTCTGCTGTTATTCTAAAATGAAAGATATGAATTCAACAAAGTTGATGGATAACTTTCTTTGACTGCTCT ACCTGAATTTAGACTAAGCAGTAAATAGTTTAATAAAAGATCACTTT 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BD127295 unedited TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTCCGCATCCAGC CTAGCGTGTCCACGATGCGGCTGGGCTCCGGGACTTTCGCTACCTGTTGCGTAGCGATCG AGGTGCTAGGGATCGCGGTCTTCCTTCGGGGATTCTTCCCGGCTCCCGTTCGTTCCTCTG CCAGAGCGGAACACGGAGCGGAGCCCCCAGCGCCCGAACCCTCGGCTGGAGCCAGTTCTA ACTGGACCACGCTGCCACCACCTCTCTTCAGTAAAGTTGTTATTGTTCTGATAGATGCCT TGAGAGATGATTTTGTGTTTGGGTCAAAGGGTGTGAAATTTATGCCCTACACAACTTACC TTGTGGAAAAAGGAGCATCTCACAGTTTTGTGGCTGAAGCAAAGCCACCTACAGTTACTA TGCCTCGAATCAAGGCATTGATGACGGGGAGCCTTCCTGGCTTTGTCGACGTCATCAGGA ACCTCAATTCTCCTGCACTGCTGGAAGACAGTGTGATAAGACAAGCAAAAGCAGCTGGAA AAAGAATAGTCTTTTATGGAGATGAAACCTGCGTTAAATTATTCCCAAAGCATTTTGTGG AATATGATGGAACAACCTCATTTTTCGTGTCAGATTACACAGAGGTGGATAATAATGTCA CGAGGCATTTGGATAAAGTATTAAAAAGAGGAGATTGGGACATATTAATCCTCCCACTAC CTGGGCTGGACCACATTGGCCACATTTCAGGGCCCAACAGCCTCCTGATTGGGCANAACC TGAGCGAGATGGACAGCGTGCTGATGAAGAACCACACCTCACTGCAGTCGAAGAGAGAGA GACGCCTTACCCATTTGCTGGGTCTTTGTGGGACCATGGCATGTCTGAACAGAGTCCGCG CCTCCTCCCGAGAGGGATACCTTGTTC 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BD127295 unedited TGGTCAATATACGGACTCGGGTTGATTAANNCCGCCCCTTTTAAGTGATCTTTTTTAAAC TATTTACTGCTAAGACTACCTTCAGGTAGAGCAGTCAAAGAAAGTTATTTCTCAACTTTG TTGAATTCATATCTTTCATTTTAGAATAACAGCAGACTTTAAGCATGTATTATTTTTCCA GTGTTCATTTTAGTCTAAGACTGTTTGAGTCTGGTTTGATCCATTGCTCGTGGAAGAATA CACAGACAGCAGCTGTAATGAGCAGGTGCATTCCCTCGTAGAGAAGTTTTGGAGAAAATA CACTCCATATAAATAAATGATAACGCAGAGATGTCACCAAAACGATGTACGTGAAAACTG GAATAGAACAAATCAGTGCGTAGCAGAAGCAAGCATGACTCAGTGCTGAACCACTGCGTG TTTCTGAGCTCAGGAAGTGCACTAAGTGGCTGGCCCACAGCACAGGCCCTGCGTACGTCC CAAACGCTGTCAGGAGCACCGCTGGGATTTCCACGTAGGTGTCTAAGCCCCCGAAGCCTG CGGAGATGTCCACGGTGGCAATGTTGTTTGAGTTGCCCTGAAAATAGAAGAATGCTTGCC AAACCATAATGCATCACAGTAATCTCAGCTGGATCGTGTCTTAGGGGCTTCCAGATGAAT TTTAGCCATTAGAAGTCTGAATCAAAAGGGCTAATGCTTAAGACCCGAAGAATAATGTGT GCTAAAAAGAAAAGGTTGGCCGAAGAACCTAAACCACTATTTATTTTCCCTAAAACCCCC CAGTTCTGAGTTTTGAAGTTTTCGGCACTGGCTTTGGAGATTAAGGAAGACTTTGGGGGC CCCGGAACAGAATGCCCGGGGCAAAAACCTTTAACAAAACCACGCTTAAAAAAACCCCTT TGGAAAAATGCCTGGCTTGTCCGGCCCGCCCGCGGAACCCCGACCCCTCTCCTGGGCGCC CCGCGGCGCATATAACTCCCCAAACCCCCGCGCCCGGGGAGGCCTTGGACCT
Restriction Sites	NotI-NotI
ACCN	BD127295
Insert Size	3300 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BD127295.1
RefSeq Size	3197 bp
RefSeq ORF	3197 bp
Locus ID	54872
Protein Families	Transmembrane
Protein Pathways	Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
Gene Summary	This gene encodes an enzyme involved in glycosylphosphatidylinositol-anchor biosynthesis. The encoded protein, which is localized to the endoplasmic reticulum, is involved in transferring ethanoloamine phosphate to mannose 2 of glycosylphosphatidylinositol species H7 to form species H8. Allelic variants of this gene have been associated with intellectual disability, hypotonia, and early-onset seizures. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]

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