KCTD1 (AL831954) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	C18orf5; SENS
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL831954, the custom clone sequence may differ by one or more nucleotides AGGTGAAAGACAGTGAATAAAAGAATTCATTTGACAAAGCCTAGGACTATTTTGAATGGTCTCGTCCTGA GATATTTATCAAGCGGGGACCAACACTTTGCAGACAATCGTAATTTTTCCCTACTGCCATCTTCAAAAGT GCTACCTTTGACAGTGATAAAAGACACCTCTTAGTCCTAGTGCCCGAACAATTGATCTAATTGTCTTCAC TGTTCCCTCTTTTCTATCACAGAATCGGAAGACTTTTTGATGGTACAGAGCCCATTGTTTTGGACAGTCT CAAGCAGCACTATTTCATTGACAGAGATGGACAGATGTTCAGATATATCTTGAATTTTCTACGAACATCC AAACTCCTCATTCCTGATGATTTCAAGGACTACACTTTGTTATATGAAGAGGCAAAATATTTTCAGCTTC AGCCCATGTTGTTGGAGATGGAAAGATGGAAGCAGGACAGAGAAACTGGTCGATTTTCAAGGCCCTGTGA GTGCCTCGTCGTGCGTGTGGCCCCAGACCTCGGAGAAAGGATCACGCTAAGCGGTGACAAATCCTTGATA GAAGAAGTATTTCCAGAGATCGGCGACGTGATGTGTAACTCTGTCAATGCAGGCTGGAATCACGACTCGA CGCACGTCATCAGGTTTCCACTAAATGGCTACTGTCACCTCAACTCAGTCCAGGTATAGCATTGTTACGC ACTGTGTGGCATCAAATGCCACTCTTACTGTTGTCACTTAACCTTGCCCTCACATGGTGCCTTTCCTCCA AAGAGCTGAGAAACTCACTCAGTGACACTTCTGTTGGGTAGGTGAATTATCACCCTCTATAGCAGTCATT CCGTAAGTCTTGTGATTGTGCCAGGTGCTGGGATGAGCACTGGAGATACAGAAGAGACAGATATAGAACA CGACAGCTGGGCACGGTGGCACACGCCTATAGTCCCAGCCACTCCAGAGGCTAAGGCAGGAGGATCACTT GAGCTCAGGAGTTCAGGTTCAGCCTGGGCAACATAGCGAGACAGACAGACACAGAACACGATGGACAGCT CTGTGTTCATGGAACTGCATTATGGGAGTGGAGTGGGAGGCAAACAGTCAACATAAATATATTGCCCAGG AGGTGGTAAGTGGATTTCTGGGGCAAAGTATGCTGGCATATTGAGGACTATCAGGAAAGCCAGTTTGGCT TGAAGAAAGTGACTCAGGGAGAGAGAGTACCCAGACAGGAGGCCCGGGAAAATCAGGGACAGATCACGGA GAGCCTCGTTAGGAGGTGGCAAGTGCCCTAGGATTGTTTATTTTGGCATCTTTTGGTTTTGGTTCGTTTG TGTATGTGAGCCAAATGGAAATCCATTGGAGGGTTTTGAGCAGAAGGGAGAACTGACAGAACTCGTATTT TAAAGTGAACATTGCGACAGCAGCTAGAGAATAGGAGAAACAAGGAGACCAGGCAGCAAGCTGTGGTTCA AGCAGTTGAAAAGTGTCCAGTTGTGTGTGTCTGGAGGGTAGAGCTAATAAGGTTTTCTGACGATGGCTGT GGGTTGTGAGACATAAGCCCGGGATGACTAAGATTTTTGACAGAGGAACTGGGTGAGTGGAACTGCCATT TCCTGAGATGGAGAAGTGTAGGGGAGCAAGAGATGTGGGGGTGGAATTGAGAACTCAGAGGCTAATGTGT GCTGAGCACCGACTCTCTGCCCTACCCTGGGCCATGGCCTGAGCATGTGTTGTCTTCTTACAACCCTAAC AGGTAAGCACTGTTAATCCTCTCTGTCTTGCTGATGGGAAGGCAGAGGCACACCTGGGTCTCGATTCCAG CTCCGTGTGACACAAAACCATTTGAAGTCAATGGAGTGAGGGTTCTAGTTTTTTCCCTCTTTTTAACTGG AATGGGCCCATGACTTTGTTCAGTATTTAGTCAGCAGAACCCTTCCCAAAGCACAGAGACTAGAAGCACC CACTCTTCTGCGCTGCCTCTTGAGGATTGTTCCACTACTATTTATAACTCTTTAAAATATATGAACAGAT GTCTGAGAACAGAAGAGCAGAAAACTGGCAGAAAATGTTCCCTAAGCATGCTAGCGGAAGGCACATGCTC CCAGGCGGCGGGCTGGCTGCTGGGTCGCAGGGTTCCCGAGGACGAGATTCGCGTTCTCACCTGTGCGGAG AGCGTCCCCTGGAGGTGTGTGGTGCTCCCGCATGGCCCTGCACAGCGTTCCTGAGACGGCCTCAGCAGTT TAAGCCTCTTTAAAGCCCAGAAAGCCTGTCCCCTTCCACACAACAGCCTGCCCTGGCTATCACTGGAGCA GACAGAGGGGCAGAACCATTCAGAGCCAGGACAGGAAGGCCTCGGCCACACCTTTGGGCCAGTGAATAAC GATCATCAATTCATGTGCTTGACAGTTTCCCACATCAGATATGGAAATTTTCATCTGGATTAACGTGGTC ATATGACACAGCCATGACCTACCCTTGCGTCCTGCTCCACCGACCAAGAAATAGGCACCAGCCTATGGGC ACGCAGAGACTCTGGGATAAGGCTTACGACACCCGATACCTCGCTAAGTTGTTGTGTGATCACTGTTTAA GGAACTTTGCTGTGAACGTGTTGTTGTTTCCAAAGTAATGGCTCCATCAGCTGTTTGCATTACATTGAAA TAAGCTTTTGACCACATCCAAGGTAACATTGGATCACATTGAATAATTATCACATGATGCCTATCGGACT CCGAAGAAGAGCTTAGCGATTTCCTCCTGTTAGCATTTAGTGACCTTAAGACTCAAAGAAAAATGGACAT CAGGGTTAGGTTCTCTGCTGTTCATGGCTTCCTCCCCTTTGGATTTCCTCTCCTAATCATGGCAAGGGAT CTTTTCCAGGGTGCTGGTTTGGCCAAGCAAGCTGGGATGCCTCTTGCTGAGGGCTGCATTCCTGCCTCTG CTGGGGAATCCATGGAGGGAGCTGTTCTTCGTCTTTGTATTTACCTGCCACCAAAGCACATCTTGATTTT GAATTTAGGGATTGTTTTCTTCCTGTCCTTATGCTGACAGGTATGCATATGCATTGTGAATTTACCTACA GAAATAGAGCACTGCTGGGTAGAATGTTAATTTTGTTCTTATAAGGACTTGTGTATGTTTTTTTAATTTG CCAGCACTTGAGGTTTTAATTAATTTTTAGAAAACATACTCTCTTGCTTTCCCATCTCATCTAGTAAGCT GTTTTGCAACATAGCAGACTATATTCTATATTGAAGTAGGCAACTAAAAAATAATACTTTTTAAAAAAGT AAGGATTCACTTTACATTGACTACATTTCAGTTCATTCCAATAAAAGTTTTTTTGAGGTGGGTTTTTTTG TTTTGTTTTTTTTTTTTAAGTTATGATTGAATCACTTTGCCTTTAAGGTGGTGTGGAAGTAATTTAATTA TATTCAGAACTCGGGCAGGAAACATTTGCAAGTCTAATATTTGTTGGATTCCTAAAGAAACAGAATGCTC CAGATCAGTTTCCTGTATTTTTGTCTATCTAATCTAAGAGTCACAGGTACTGTTTCCTGCATTTAACAGT GTCAGACATGTAGTGCCAAACCCAGGAATCTTGCTGGCGTTAGGTTTTTGATTTCAAAATTAAGGAACAG ATTCTATGGCTTACATTTGTATAAGAAGTTTAGAATATCTTTATTTTTCTTACATTTTCTAATATGTAAT CATTGTGCTGGTTCTTGTCTCCTTGAATGAATGCAGGGCAACAAATCTGTTTCAGATGTTACAAACTTGA AAACCTGTAGATAATCCTCTAGTGTCTTCAGTAATCCCTTTGTTCTGCTATTGAAATTACATCAATCTTC CAGGCCAAAACATATATATTAACTCGGTATTAGGAAAACTTGAACAAAGGCGGAGTTGAGAGTACAAATG AATCACTGACTAAGTAAACTCTGCCATCACTGTCCTTTCCAGATTCTTTATCATCTCATAGGATGTATTT TAGTGATAAATGAGCTTTGAAATGTGAACCCTCATTTTTGACAGAGAGGGCAGCATTGCATATACAAAAA CAGGAGATTTGAGGCCAAACAGACCTGAGTTGGAAACCCAGTCCCATCACTGACTAGCCATGAACCTCAG TTTTCTCATTTATAAAATGGGCCTAACACCATCGAGTGTTGTAGCTGGTGTGTGTAAAGCAAGCATGAAA GGCACATTTTAGGCATTAGAAATGCTTGCTTCCTCTCTCCTGCCCATTAATCCAGGGTCTTCCTTTGAGA GGAGCAGCTCACAGGGACATGGGTGCTGGATGAGATGAACCAGAGTGCGCATAATCTCAAACTTTGGGGT GTTTATGTAGCCATAGGACTTACCTCACCCCTAACTGGATGCTTGTCTTGTTCCCTCGCAGGTCCTCGAG AGGTTGCAGCAAAGAGGATTTGAAATCGTGGGCTCCTGTGGGGGAGGAGTAGACTCGTCCCAGTTCAGCG AATACGTCCTTCGGCGGGAACTGAGGCGGACGCCCCGTGTACCCTCCGTCATCCGGATAAAGCAAGAGCC TCTGGACTAAATGGACATATTTCTTATGCAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL831954 unedited GGTCACATTTAGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGTCAAGACC TCTGATCACTAGATCCCCTGCATCTCCACTGAACAACCAAGGCATCCCTACTCCAGCACA ACTCACAAAATCCAATGCGCCTGTCCACATTGATGTGGGCGGCCACATGTACACCAGCAG CCTGGCCACCCTCACCAAATACCCTGAATCCAGAATCGGAAGACTTTTTGATGGTACAGA GCCCATTGTTTTGGACAGTCTCAAACAGCACTATTTCATTGACAGAGATGGACAGATGTT CAGATATATCTTGAATTTTCTACGAACATCCAAACTCCTCATTCCTGATGATTTCAAGGA CTACACTTTGTTATATGAAGAGGCAAAATATTTTCAGCTTCAGCCCATGTTGTTGGAGAT GGAAAGATGGAAGCAGGACAGAGAAACTGGTCGATTTTCAAGGCCCTGTGAGTGCCTCGT CGTGCGTGTGGCCCCAGACCTCGGAGAAAGGATCACGCTAAGCGGTGACAAATCCTTGAT AGAAGAAGTATTTCCAGAGATCGGCGACGTGATGTGTAACTCTGTCAATGCAGGCTGGAA TCACGACTCGACGCACGTCATCAGGTTTCCACTAAATGGCTACTGTCACCTCAACTCAGT CCAGGTCCTCGAGAGGTTGCAGCAAAGAGGATTTGAAATCGTGGGCTCCTGTGGGGGAGG AGTAGACTCGTCCCAGTTCAGCGAATACGTCCTTCGGCGGGAACCTGAGCNGACGCCCCG TGTACCCTCCGTCATCCGGATAAGCAAGAGCCTCTGGACTAATGGACATATTNCTTATGC AAAAAGAAAACACACACACCAATAACTCAAACAAAAAGGGACATTTATGTGCAGTTGGGA CAGCAAACAAGTNCTGGACGTAAAATCGAATAAAAGAACATTNATATCC
Restriction Sites	NotI-NotI
ACCN	AL831954
Insert Size	1700 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AL831954.1
RefSeq Size	4596 bp
RefSeq ORF	4596 bp
Locus ID	284252
Protein Families	Ion Channels: Other
Gene Summary	This gene encodes a protein containing a BTB (Broad-complex, tramtrack and bric a brac), also known as a POZ (POxvirus and zinc finger) protein-protein interaction domain. The encoded protein negatively regulates the AP-2 family of transcription factors and the Wnt signaling pathway. A mechanism for the modulation of Wnt signaling has been proposed in which the encoded protein enhances ubiquitination and degradation of the beta-catenin protein. Mutations in this gene have been identified in Scalp-ear-nipple (SEN) syndrome. [provided by RefSeq, May 2017]

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