PCGF1 (AL049296) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	2010002K04Rik; NSPC1; RNF3A-2; RNF68
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL049296, the custom clone sequence may differ by one or more nucleotides TGGCACCTTCTTCTAGGTGAAGAGAAACGGATTCGGGAATTCTACCAGTCCCGAGGTTTGGACCGGGTCA CCCAGCCCACTGGGGAAGGTATGTCCTTGGCCGCGGGACAGTAAAGACCCCAGAGCATTCTTCTTGCCCA GTTTTGCTCTCTGGGGAAAGAGGAGTATGGAATGTGTGCCACCAGCCACCTCACTACCCTATCTTTCTCA GAGCCAGCACTGAGCAACCTCGGCCTCCCCTTCAGCAGCTTTGACCACTCTAAAGCCCACTACTATCGCT ATGATGAGCAGTTGAACCTGTGCCTGGAGCGGCTGAGGTGAGGAGAAGGTCAGGGGTTGCAGGAGGTGAC AGTGCCAATGACCCAGAGCCAGGGAGGGTCTAGGGGAGAGGCTGAGCAGTGAGTGAGTGCCTATCCCCTT GAAGAGAGTATATCATGGCTCTGGGTGGGGAAGAGGAGGAAAGATAGGATTCCCTAACCTGTGTCTATTT CCCCCCAGTTCTGGCAAAGACAAGAATAAAAGCGTCCTGCAGGTGAGAAGGGCTGAGGGGAGGGCCTCTC TAAGGAGACTCACCTCCCATGGTCCTTCCCTCACACACCTTGCCCTCTTCCCTCCCCTCCCTGCTCCCAG AACAAGTATGTCCGATGTTCTGTTAGAGCTGAGGTACGCCATCTCCGGAGGGTCCTGTGTCACCGCTTGA TGCTAAACCCTCAGCATGTGCAGCTCCTTTTTGACAATGAAGTTCTCCCTGATCACATGACAATGAAGCA GATATGGCTCTCCCGCTGGTTCGGCAAGGTAAGCCAGGCCACCCTCCCTGGGATCACACCCCCTTCAGAC TCCCCCCAACCATCCTACAGTCCTCAGGGGAAGGGTGGGCTGAGGGGCCCTTTGAATAATATAAGAACAT TCCCCACTGACTACTACTTCCTCATTCTCTCCTTAGCCATCCCCTTTGCTTTTACAATACAGTGTGAAAG AGAAGAGGAGGTAGGGGCCAAGCCCCCACCCCATCCCACTCCCCTTCCCTCCCCAGATATTTATGTGAAA TGAACTGCAGCTTTATTTTTTGAAATAAAAACTTTTAAAAAGCAAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL049296 unedited GTCACATTATGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCCTCGTGCCG AATTCGGCACGAGGGGATGCCACCACCATCACAGAGTGTCTTCATACTTTCTGCAAGAGT TGTATTGTGAAGTACCTCCAAACTAGCAAGTACTGCCCCATGTGCAACATTAAGATCCAC GAGACACAGCCACTGCTCAACCTCAAACTGGACCGGGTCATGCAGGACATCGTGTATAAG CTGGTGCCTGGCTTGCAAGACAGTGAAGAGAAACGGATTCGGGAATTCTACCAGTCCCGA GGTTTGGACCGGGTCACCCAGCCCACTGGGGAAGAGCCAGCACTGAGCAACCTCGGCCTC CCCTTCAGCAGCTTTGACCACTCTAAAGCCCACTACTATCGCTATGATGAGCAGTTGAAC CTGTGCCTGGAGCGGCTGAGTTCTGGCAAAGACAAGAATAAAAGCGTCCTGCAGAACAAG TATGTCCGATGTTCTGTTAGAGCTGAGGTACGCCATCTCCGGAGGGTCCTGTGTCACCGC TTGATGCTAAACCCTCAGCATGTGCAGCTCCTTTTTGACAATGAAGTTCTCCCTGATCAC ATGACAATGAAGCAGATATGGCTCTCCCGCTGGTTCGGCAAGCCATCCCCTTTGCTTTTA CAATACAGTGTGAAAGAGAGNAGAGGTAGGGGCCAAGCCCCCACCCCATNCCACTCCCCT TNCCTCCCCAGATATTTATGTGAAATGAACTGCAGCTTTATTTTTTGAAATAAAACTTTT AAAAGCAAAAAAAAAAAAAAACTCGACTTAGATTGGGCCGCGGTCATAGCTGTTTCCTGA AAGATCCCGGTGGCATCCCTGTGACCCTNCCCATGCCTCTCCTGGCCCTGGAAGTGCCAC TCATGCCCACCACCTTGTCCTAAAAATAAGTGCATCATTTGTTGA
Restriction Sites	NotI-NotI
ACCN	AL049296
Insert Size	800 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL049296.1
RefSeq Size	1113 bp
RefSeq ORF	1113 bp
Locus ID	84759
Gene Summary	PCGF1 is a mammalian homolog of the Drosophila polycomb group genes, which act as transcriptional repressors to regulate anterior-posterior patterning in early embryonic development (Nunes et al., 2001 [PubMed 11287196]). See also PCGF2 (MIM 600346).[supplied by OMIM, Aug 2008]

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