C1orf187 (DRAXIN) (BC021286) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AGPA3119; C1orf187; neucrin; UNQ3119
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC021286, the custom clone sequence may differ by one or more nucleotides CAGGTGCTGGATGCAGCCATGGAGGAATCCTCCACCAGCCTGGCGCCCACCATGTTCTTTCTCACCACCT TTGAGGCAGCACCTGCCACAGAAGAGTCCCTGATCCTGCCCGTCACCTCCCTGCGGCCCCAGCAGGCACA GCCCAGGTCTGACGGGGAGGTGATGCCCACGCTGGACATGGCCTTGTTCGACTGGACCGATTATGAAGAC TTAAAACCTGATGGTTGGCCCTCTGCAAAGAAGAAAGAGAAACACCGCGGTAAACTCTCCAGTGATGGTA ACGAAACATCACCAGCCGAAGGGGAACCATGCGACCATCACCAAGACTGCCTGCCAGGGACTTGCTGCGA CCTGCGGGAGCATCTCTGCACACCCCACAACCGAGGCCTCAACAACAAATGCTTCGATGACTGCATGTGT GTGGAAGGGCTGCGCTGCTATGCCAAATTCCACCGGAACCGCAGGGTTACACGGAGGAAAGGGCGCTGTG TGGAGCCCGAGACGGCCAACGGCGACCAGGGATCCTTCATCAACGTCTAGCGGCCCCGCGGGACTGGGGA CTGAGCCCAGGAGGTTTGCACAAGCCGGGCGATTTGTTTGTAACTAGCAGTGGGAGATCAAGTTGGGGAA CAGATGGCTGAGGCTGCAGACTCAGGCCCAGGACACTCAACCCCAGGAGGGGAGCCGCTCGGCGAATGAG CTGGGTGGGTGCCCAGGAGCCGGCCCGCAGCACCTGCACACACGAAGTCCGGACCCACGCAGCCTCCATC CCGCGTGTCTTGCTCTCCGCGATGGCAATGCCGAGAGTGCCCTCTACTGTCCGACTCCAGCACTGCAACA GCTTCAAGTTCAAAACCAAGAGGCGTTTTTGAGAGTGGAAAAGAAATTTAAACTTCCCGAAAGAAGGTCC ACCATCAGGAGATGAATATGGAACATCTCCTATGTACCAGGCACTGTGCTAAGTGCTGGAATACATTATC TCATTGAATTATCACAACGACCTTTTCAAGTAGGCATTATCACCATGCGACAGGTTGAGGACCGTGAGGC ACGCTGGAGTTATTTGACTCGACTTGCCCAGGGTCCCAGGTCAGTGCTCCTCAAACCTGAACATGCTAAG AGTCACCTGGAGGTCCAGTTAAAAATGTACATCTGGCCGGGCGCGGCGGCTCACGCTTGTAATCCCAGCA CTTTGGGAGGCCAAGGCGGGTGGCTCACCTGAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGGGAA GCCCCGTCTCTATTAAAAATACAAAAATTAGGCCAGGCGCGGTGGTGCACACCTGTAATCCCAGCTACTC GGAGGCTGAGGCACCAGAATCGCTTGAACTTGGGAGGCAGAGGTTGCAGTGGGCGGAGATCGTGCCACTG CACTCCAGCCTGGGGGACAAGAGTGAAACTCCATCTCAAAAAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC021286 unedited NGGGGGTTCATATTTGTATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGCCAGG ATGGGGCTGTGGTCACCGCCACCAGGCAGGCCTCCAGGCTGCCAGAGGCTGAGGGGCTGC TGCCTGAGCAGAGTCCTGCAGGCCTGCTGCAGGACAAGGACCTGCTCCTGGGACTGGCAT TGCCCTACCCCGAGAAGGAGAACCGACCTCCAGGTTGGGAGAGGACCAGGAAACGCAGCA GGGAGCACAAGAGACGCAGGGACAGGTTGAGGCTGCACCAAGGCCGAGCCTTGGTCCGAG GTCCCAGCTCCCTGATGAAGAAGGCAGAGCTCTCCGAAGCCCAGGTGCTGGATGCAGCCA TGGAGGAATCCTCCACCAGCCTGGCGCCCACCATGTTCTTTCTCACCACCTTTGAGGCAG CACCTGCCACAGAAGAGTCCCTGATCCTGCCCGTCACCTCCCTGCGGCCCCAGCAGGCAC AGCCCAGGTCTGACGGGGAGGTGATGCCCACGCTGGACATGGCCTTGTTCGACTGGACCG ATTATGAAGACTTAAAACCTGATGGTTGGCCCTCTGCAAAGAAGAAAGAGAAACACCGCG GTAAACTCTCCAGTGATGGTAACGAAACATCACCAGCCGAAGGGGAACCATGCGACCATC ACCAAGACTGCCTGCCAGGGCTGCGCTGCTATGCCAAATTCCACCGGAACCGGCAGGTTA CACCGGAGAAAAGGCGCTGTGTGGAGCCCGAGACGGCCAACGGCGACCAGGGATCCTTTA TCAACGTCTAGCGCCCCCGCGGGACTGGGGACTGAGCCCCAGAGTTTGCACAGCCGGGCG ATTTGTTGTAACTAGCAGTGGGAGACCAAGTTGGGAACAAATGCTGAAGCTGCAGACTCA GGCCCAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC021286 unedited TACAGCTATGNACCGCGGGCCGCAATCTAGAGTACGAGTTTTTTTTTTTTTTTTTTGAGA TGGAGTTTCACTCTTGTCCCCCAGGCTGGAGTGCAGTGGCACGATCTCTGCTCACTGCAA CCTCTGCCTCCCAAGTTCAAGCGATTCTGGTGCCTCAGCCTCCGAGTAGCTGGGATTACA GGTGTGCACCACCGCGCCTGGCCTAATTTTTGTATTTTTAATAGAGACGGGGTTTCCCCA TGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGAGCCACCCGCCTTGGCCTCCCA AAGTGCTGGGATTACAAGCGTGAGCCGCCGCGCCCGGCCAGATGTACATTTTTAACTGGA CCTCCAGGTGACTCTTAACATGTTCAGGTTTGAGGAGCACTGACCTGGGACCCTGGGCAA GTCGAGTCAAATAACTCCAGCGTGCCTCACGGTCCTCAACCTGTCGCATGGTGATAATGC CTACTTGAAAAGGTCGTTGTGATAATTCAATGAGATAATGTATTCCAGCACTTAGCACAG TGCCTGGTACATAGGAGATGTTCCATATTCATCTCCTGATGGTGGACCTTCTTTCGGGAA GTTTAAATTTCTTTTCCACTCTCAAAAACGCCTCTTGGTTTTGAACTTGAAGCTGTTGCA GTGCTGGAGTCGGACAGTATAGGCACTCTCCGCATTGCCATCGCGGAGAGCAAAACACCC GGGATGGAGGCTGCGTTGGTCCCGACTTTATGTGTGCAAGTGCTGCAGGCCGGTCCCTGG GACCCACCCAGCTCAATCCCCAAACGCTTCCCTCCCTGGGGTGAATGTCCCGGGCCCAAA TTTGCACCCTCAACCTCCGTTCCCAAATTTGTTTCCCATTGCTATTTACAAAAA
Restriction Sites	NotI-NotI
ACCN	BC021286
Insert Size	1750 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC021286.1, AAH21286.1
RefSeq Size	1465 bp
RefSeq ORF	333 bp
Locus ID	374946
Protein Families	Secreted Protein, Transmembrane
Gene Summary	Chemorepulsive axon guidance protein required for the development of spinal cord and forebrain commissures. Acts as a chemorepulsive guidance protein for commissural axons during development. Able to inhibit or repel neurite outgrowth from dorsal spinal cord. Inhibits the stabilization of cytosolic beta-catenin (CTNNB1) via its interaction with LRP6, thereby acting as an antagonist of Wnt signaling pathway.[UniProtKB/Swiss-Prot Function]

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