SCYL2 (BC012387) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	coated vesicle-associated kinase of 104 kDa; CVAK104; FLJ10074; KIAA1360; SCY1-like 2 (S. cerevisiae); SCY1-like 2 protein
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC012387, the custom clone sequence may differ by one or more nucleotides AGATTTTGTTAATTTTCCTACAAAAAATGGATTTGCTACTAACCAAAACCCCTCCTGATGAGATAAAGAA CAGTGTTCTACCCATGGTTTACAGAGCACTAGAAGCTCCTTCCATTCAGATCCAGGAGCTCTGTCTAAAC ATCATTCCAACCTTTGCAAATCTTATAGACTACCCATCCATGAAAAACGCTTTGATACCAAGAATTAAAA ATGCTTGTCTACAAACATCTTCCCTTGCGGTTCGTGTAAATTCATTAGTGTGCTTAGGAAAGATTTTGGA ATACTTGGATAAGTGGTTTGTACTTGATGATATCCTACCCTTCTTACAACAAATTCCATCCAAGGAACCT GCGGTCCTCATGGGAATTTTAGGTATTTACAAATGTACTCTTACTCATAAGAAGTTGGGAATCACCAAAG AGCAGCTGGCCGGAAAAGTGTTGCCTCATCTTATTCCCCTGAGTATTGAAAACAATCTTAATCTTAATCA GTTCAATTCTTTCATTTCCGTCATAAAAGAAATGCTTAATAGATTGGAGTCTGAACATAAGACTAAACTG GAGCAACTTCATATAATGCAAGAACAGCAGAAATCTTTGGATATAGGAAATCAAATGAATGTTTCTGAGG AGATGAAAGTTATAAATATTGGGAATCAGCAAATTGACAAAGTTTTTAACAACATTGGAGCAGACCTTCT GACTGGCAGTGAGTCCGAAAATAAAGAGGACGGGTTACAGAATAAACATAAAAGAGCATCACTTACACTT GAAGAAAAACAAAAATTAGCAAAAGAACAAGAGCAGGCACAGAAGCTGAAAAGCCAGCAGCCTCTTAAAC CCCAAGTGCACACACCTGTTGCTACTGTTAAACAGACTAAGGACTTGACAGACACACTGATGGATAATAT GTCATCCTTGACCAGCCTTTCTGTTAGTACCCCTAAATCTTCTGCTTCAAGTACTTTCACTTCTGTTCCT TCCATGGGCATTGGTATGATGTTTTCTACACCAACTGATAATACAAAGAGAAATTTGACAAATGGCCTAA ATGCCAATATGGGCTTTCAGACTTCAGGATTCAACATGCCCGTTAATACAAACCAGAACTTCTACAGTAG TCCAAGCACAGTTGGAGTGACCAAGATGACTCTGGGAACACCTCCCACTTTGCCAAACTTCAATGCTTTG AGTGTTCCTCCTGCTGGTGCAAAGCAGACCCAACAAAGACCCACAGATATGTCTGCCCTTAATAATCTCT TTGGCCCTCAGAAACCCAAAGTTAGCATGAACCAGTTATCACAACAGAAACCAAATCAGTGGCTTAATCA GTTTGTACCTCCTCAAGGTTCTCCAACTATGGGCAGTTCAGTAATGGGGACACAGATGAACGTGATAGGA CAATCTGCTTTTGGTATGCAGGGTAATCCTTTCTTTAACCCACAGAACTTTGCACAGCCACCAACTACTA TGACCAATAGCAGTTCAGCTAGCAATGATTTAAAAGATCTTTTTGGGTGAGGTGTCTTACTTCTATTTTG AAGGATTATTTCAGTTTCAATCATGGGTGAGCTGATTTACATCTTTATATAGTTGGCTTGGAGGAAGTAC TTCTATGGGAAAGTGAACAGTTCTGTGACAGGAAACATCTCTGTCCATGCCAGCATAGTAGTTGTATGGA CTTCTAACCAGTTGAGTTTTTTAAAGCATTGAGGATTTTTTCCTCTTACCAACTCCTCTTCAGGTTTTTA AAGACCCAGCCCTTCCCAATCTCAAAGAGAAAAAGGAAACTGAGTTATCTTGAATAACATAACTTTTTAA TCAAATGTTTATTTTGGCTTGTGGATCTTGGTGTTATTTAAAAAATTGAGGTGATGGTCATTGCAAGCTC ATCTATTAAGTACTATATGGTACACAGTCTATGAGTCATTAGTCTTCATTTTAATATGTAAAAAATCTTG ATGCTGTATTGATTTGTTTGCATTTAAGATGACAGTGAGAAAATGATAAGCATAAAGAGAAGTATCAGGT TATTTGCTTTTTCCAAACTTTTCAGATGAACTATTGTTTAGTACAGAGACTGAGCAAATACTACAAAATT CAACTTAACCTTCATTTCATTGGTTTAAATGCGTTATTAACCATCTTAAGTGCAAACTAATCATTGTAAA TTATATTTTAGCATGGTCTGCCTCAAATAGTAATGTATTTTTCTGCATTCACTTGGATATATTTAGAATC ACTTTTTTCCTCCTGTATCAAGGAAGAGGTATGTGCTGATTTGTTTGGATATTTGACAAGGCACTCTGAT GTGACTTCCCTGACTACTACCTTCATATTTCATTTCAAATTCAAACTTCTGAGGTTGCAGCATATATGAA TTGCATTTTCAAAAGAAGATTTGTAAGAATTAAACTATATTTATGAGTAAACTTTTGAGGTTTCTGCTGT ATTGTTTCAAATGTAATAAACTTTACTTCTGTAAAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC012387 unedited TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTCGTGAACATGTA AAGCTACTGTTAAATGTAACTCCGACTGTAAGACCAGATGCAGATCAAATGACAAAGATT CCCTTCTTTGATGATGTTGGTGCAGTAACACTGCAATATTTTGATACCTTATTCCAAAGA GATAATCTTCAGAAATCACAGTTTTTCAAAGGACTGCCAAAGGTTCTACCAAAACTGCCC AAGCGTGTCATTGTGCAGAGAATTTTGCCTTGTTTGACTTCAGAATTTGTAAACCCTGAC ATGGTACCTTTTGTTTTGCCCAATGTTCTACTTATTGCTGAGGAATGCACCAAAGAAGAA TATGTCAAATTAATTCTTCCTGAACTTGGCCCTGTGTTTAAGCAGCAGGAGCCAATCCAG ATTTTGTTAATTTTCCTACAAAAAATGGATTTGCTACTAACCAAAACCCCTCCTGATGAG ATAAAGAACAGTGTTCTACCCATGGTTTACAGAGCACTAGAAGCTCCTTCCATTCAGATC CAGGAGCTCTGTCTAAACATCATTCCAACCTTTGCAAATCTTATAGACTACCCATCCATG AAAAACGCTNTGATACCAAGAATTAAAAATGCTTGTCTACAAACATCTTCCCTTGCGGTT CGTGTAAATTCATTAGTGTGCTTANGAAAGATTTTGGAATACTTGGATAAGTGGTTTGTA CTTGATGATATCCTACCCTTCTTACAACANATTCCATCCAAGGAACCTGCGGTCCTCATG GGAATTTTAGTATTACAAATGTACTTTTACTCATAAGAANNGTGGGATCACCAAAGAGCA GCTGGCCGGNAAAGTGGTGCCTCATCTTATCCCCTGAGTATGAAAACATCTAATCTTATC AGTTCAATCTTTCATTCCGTCTAAAGAAAGCTTATA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for BC012387 unedited CCCCGGTTATTATCTATGNNACCGCGGCCGCATNCTAGNGATCGATTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTAATTTTTACAGAAGTAAAGTTTATTACATTTTGAAACAA TACAGCAGAAAACCTCAAAAGTTTACTCATAAATATAGTTTAATTCTTACAAATCTTCTT TTGAAAATGCAATTCATATATGCTGCAACCTCAAAAGTTTGAATTTGAAATGAAATATGA AGGTAGTAGTCAGGGAAGTCACATCAGAGTGCCTTGTCAAATATCCAAACAAATCAGCAC ATACCTCTTCCTTGATACAGGAGGAAAAAAGTGATTCTAAATATATCCAAGTGAATGCAG AAAAATACATTACTATTTGAGGCAGACCATGCTAAAATATAATTTACAATGATTAGTTTG CACTTAAGATGGTTAATAACGCATTTAAACCAATGAAATGAAGGTTAAGTTGAATTTTGT AGTATTTGCTCAGTCTCTGTACTAAACAATAGTTCATCTGAAAAGTTTGGAAAAAGCAAA TAACCTGATACTTCTCTTTATGCTTATCATTTTCTCACTGTCATCTTAAATGCAAACAAA TCAATACAGCATCAAGATTTTTTACATATTAAAATGAAGACTAATGACTCATAGACTGTG TACCATATAGTACTTAATAGATGAGCTTGCAATGACCATCACCTCAATTTTTTAAATAAC ACCAAGATCCACAAGCCAAAATAAACATTTGATTAAAAAGTTATGTTATTCAAGATAACT CAGTTTCCTTTTTCTCTTTGAGATTGGGGAGGGCTGGGTCTTTAAAAACCTGAAGAGGAT TTGTAAGAGGAAAAAATCCTCATGCTTTTAAAACTCAACTG
Restriction Sites	NotI-NotI
ACCN	BC012387
Insert Size	3200 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC012387.1, AAH12387.2
RefSeq Size	2523 bp
RefSeq ORF	564 bp
Locus ID	55681
Protein Families	Druggable Genome, Protein Kinase
Gene Summary	The protein encoded by this gene associates with clathrin-coated complexes at the plasma membrane and with endocytic coated vesicles. The encoded protein phosphorylates the beta2 subunit of the plasma membrane adapter complex AP2 and interacts with clathrin, showing involvement in clathrin-dependent pathways between the trans-Golgi network and the endosomal system. In addition, this protein has a role in the Wnt signaling pathway by targeting frizzled 5 (Fzd5) for lysosomal degradation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Dec 2015]

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