BPTF (AL833375) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FAC1; FALZ; NURF301
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL833375, the custom clone sequence may differ by one or more nucleotides CCCACGCGTCCGGACTGTACTCCCAGGCCCAGGCCAGCAGCTAATGCAAGCTGCAATGCCAAATGGTACT GTTCAGCGATTCCTCTTTACCCCATTGGCAACAACAGCCACCACAGCCAGCACCACCACCACCACTGTTT CCACGACAGCAGCAGGTACAGGTGAACAAAGGCAGAGTAAACTGTCACCCCAGATGCAGGTACATCAAGA CAAAACCCTGCCACCAGCTCAGTCATCAAGTGTGGGTCCAGCAGAAGCCCAGCCACAGACTGCTCAGCCT TCAGCTCAGCCCCAGCCCCAAACCCAGCCCCAGTCCCCAGCTCAGCCTGAAGTTCAGACTCAGCCTGAAG TTCAGACCCAAACAACTGTTTCATCCCATGTCCCTTCTGAAGCACAACCCACCCACGCACAGTCATCCAA GCCCCAAGTTGCAGCACAGTCTCAGCCTCAAAGTAATGTCCAAGGACAGTCTCCTGTTCGTGTCCAAAGT CCATCACAGACTCGAATACGTCCATCAACTCCATCCCAACTGTCTCCTGGACAACAATCCCAGGTTCAGA CTACAACCTCACAACCGATTCCAATTCAACCACATACATCTCTTCAGATACCTTCCCAAGGCCAGCCACA GTCACAACCCCAGGTGGTGATGAAGCATAATGCTGTAATAGAACATTTAAAACAGAAAAAGAGCATGACT CCAGCTGAAAGAGAAGAGAATCAAAGAATGATTGTCTGTAACCAGGTGATGAAGTATATTTTGGATAAGA TAGATAAAGAAGAAAAACAGGCAGCAAAAAAACGGAAGCGTGAAGAGAGTGTGGAGCAGAAACGTAGCAA GCAGAATGCCACTAAGCTGTCAGCTCTGCTCTTCAAGCACAAAGAGCAGCTCAGAGCCGAGATCCTGAAG AAGAGAGCACTCCTGGACAAGGATCTGCAAATTGAAGTGCAGGAAGAGCTGAAGAGAGACCTGAAAATTA AGAAAGAAAAAGACCTGATGCAGTTGGCTCAGGCCACAGCAGTAGCTGCACCCTGCCCCCCAGTGACACC AGCTCCTCCAGCCCCTCCAGCCCCTCCACCTTCACCTCCCCCTCCACCTGCTGTGCAACACACAGGCCTT CTGTCCACGCCCACCTTACCTGCTGCTTCCCAGAAGAGGAAGCGGGAAGAGGAAAAAGACTCCAGCTCAA AGTCCAAGAAAAAGAAAATGATCTCTACTACCTCAAAGGAAACTAAGAAGGACACAAAGCTTTACTGTAT CTGTAAAACGCCTTATGATGAATCTAAGTTCTATATTGGCTGTGATCTTTGTACTAACTGGTATCATGGA GAATGTGTTGGCATCACAGAAAAGGAGGCTAAGAAAATGGATGTGTACATCTGTAATGATTGTAAACGGG CACAAGAGGGCAGCAGTGAGGAATTGTACTGTATCTGCAGAACACCTTATGATGAGTCACAATTTTATAT TGGCTGTGATCGGTGTCAGAATTGGTACCATGGGCGCTGCGTTGGCATCTTGCAAAGTGAGGCAGAGCTC ATTGATGAGTATGTCTGTCCACAGTGCCAGTCAACAGAGGATGCCATGACAGTGCTCACGCCACTAACAG AGAAGGATTATGAGGGGTTGAAGAGGGTGCTCCGTTCCTTACAGGCCCATAAGATGGCCTGGCCTTTCCT TGAACCAGTAGACCCTAATGATGCACCAGATTATTATGGTGTTATTAAGGAACCTATGGACCTTGCCACC ATGGAAGAAAGAGTACAAAGACGATATTATGAAAAGCTGACGGAATTTGTGGCAGATATGACCAAAATTT TTGATAACTGTCGTTACTACAATCCAAGTGACTCCCCATTTTACCAGTGTGCAGAAGTTCTCGAATCATT CTTTGTACAGAAATTGAAAGGCTTCAAAGCTAGCAGGTCTCATAACAACAAACTGCAGTCTACAGCTTCT TAAAGTTCAGCGTGTTAACCTAACATAAAACACAGCAAGAATCTGGTTGTCTGAACTATTTTAAATTAAG GAGCCAGATGTTTTTAGTCAGGCTATCCTGACAAGACTTGACCTAAACTTCGTTTTTATTGGTCATAACA GTCCAATTATATTCTTGGCCAATTTTGTCCAACGGACAAGAAAAAAGCAAAGTCAACGACACCATTATCT TGTCAAGATCAGATGGTTTTACTATTGTGGCAGAAGCGAGAAAACTTTGTTTATTGAAAAAAAAAGAAAA AGAAAGCAAGAAAAAAAGATACTATGGGGTCAAGTGTAACTCCATGGAAATGCCACGTCTGCTCTTCAGT GAAGAAGCTGGTTTAGAGTCTCACAGAAAACTTTTGACTGTATTTATTTATTGTTGCAAAAAAGACGCTT TTTTATTGCTGCCCTCATTTGTCAGCTAAGTATTTTTTCTTATAAAATCCAGCCCCGGTTACATATAATC ATCTGTATCTTATCATGATTCCTGTAGGTAAAAGTACAAGACGACCTCTAGATGTCTTTTCTTTCTATGA AAGGAGCTGCTATGTACACATGTGCACACACACACAACTGGGAATCAACAATGAGTTTATTGTTCATGGT AGATTAAAATTAAGCTTGCATAAAGGTTGGGCTAAGTGGTCCTGGACTACAGACTCTGTTGCCTTGAATA TAACAGTACAATTTGTCAATTACTCTGCACCAGGCTAAAGTGAGTAAAATCTATTTGAAGGTATCTTGTT TGTAAACATTTGTCAGATTCTAATTTTTTTCTTTTGTATTAAAATTCAACTATGGATGTATATGAAACAA AATAAATGGAGATAATTTTTCTCCCACAGACAGAGGTGTCTTTGAATGTGCGCTAATGATTATCTGTAAG CCTTTGTGGGGAGGGAGGCCTGCAAGGTCATGAAAGGCAGAAGAGTCTAATTGTGCCTGGATTTCTCCAG GACAGCAGTGGCCCCTCGCTTTATCATTCCCAGTCCATTGTCATCACGTCAGAGAAAAATCTTCAGGGGT GCTAATCCTGTTGCATCAGTTGATCATACTAACGAGAACGGTAATGCGACAAGATACACATTGCCTTCAT CTGTACATTCTGTGATACCAGGCAAATTACCAATTACACACAGCTACTTATATTTTATGAAGGGCATTTT TTAGATGACCTCATCCTCTGTGTTATTTGTTGATTGGGTTTGTTTTCTGTTTGTTGGTTTGTTTGTTTCT TCCACGTAAGGAAAAGTAGTGTAAACAGTAGCGAGAAAATGGAAACCACAGAGGAAGATGTATTTTGCAT GTTTTTCCTTTCAGTGTTCTTACACGTTGTATCACTGCATTGTGGTAATAGCTTCTATAAAATCTGCCAT AGTTGGATTATGCAGCTTTGCAAAAATTTTTACTAGATTTTGCACTAACTCATATTAGCTTTGTCCTACC AACTTCTGGAATTTATCTAATTATTGTTTTTCAAAGTTTCTTTCCTTTTAATGTTTCCCTGCTATGCAAA ACCTTTCCCAGACCTCAGTTTCTTAAAAGAAAGATGTTGCTACAGTTCCCGATTCTTTCTTATTACAGGC TCAGGTGTACAGGTTATTCTGGGTTAATTTTATCTAATGAAGCCCATTCCTTTTTGTACATAAAGATGTC ACTTAAACTTATGCTTACAAACTAAAGACTAATCGCTCAATATGAAAACATGAAAAAATTTTTGCTTAAA GTATTAAGATGGAAGTAGTTAAATATGGGTTATTTTGTCCTTTTACTTTTTTAAAAAATGTTACATATTG TATGCACTGTGCTGATGCAAGAATTCTACATTTTAATGAATTATAAAATTATTCTGCATCTCATCACATC ACAGTATTTCTGTACTATTTATTCATATATATAAATATATATGGGCTTAATCATTTAAAATTTGTTGCAG CAAGAACTTTCCTACCTGTAGGCAATAGATTGCTATGTTTTTAACAAATTGTGGCAAATTCTAAACAGCA ATTCTTTTGTACGTAATAGGACATTTCATCCTAGAAAAATAAAGTAATGTTTTTGACATTGCAAAAGAAA AAAAAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL833375 unedited NGGGGTGGTTCAGAATTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGG AAAGGCAATTATTCGAACACCTGTGATGGTACAGCCAGGTGCTCCTCAGCAAGTGATGAC TCAAATCATCAGGGGGCAGCCTGTCTCCACTGCAGTCTCCGCCCCTAACACGGTTTCCTC AACACCTGGGCAGAAAAGCTTAACTTCAGCAACGTCCACTTCAAATATACAGTCTTCAGC CTCACAACCCCCTCGCCCTCAACAAGGACAAGTGAAGCTCACCATGGCTCAACTTACTCA GTTAACACAGGGCCACGGTGGCAATCAAGGTTTGACAGTAGTAATTCAAGGACAAGGTCA AACTACTGGACAGTTGCAGTTGATACCTCAAGGGGTGACTGTACTCCCAGGCCCAGGCCA GCAGCTAATGCAAGCTGCAATGCCAAATGGTACTGTTCAGCGATTCCTCTTTACCCCATT GGCAACAACAGCCACCACAGCCAGCACCACCACCACCACTGTTTCCACGACAGCAGCAGG TACAGGTGAACAAAGGCAGAGTAAACTGTCACCCCAGATGCAGGTACATCAAGACAAAAC CCTGCCACCAGCTCAGTCATCAAGTGTGGGTCCAGCAGAAGCCCAGCCACAGACTGCTCA GCCTTCAAGCTCAGCCCCAGCCCCAAACCCAGCCCCAGTCCCCAGCTCAGCCTGAAGTTC AGACTCAGCCTGAAGTTCAGACCCAAACAACTGTTTCATCCCATGTCCCTTCTGAAGCAC AACCCACCCACGCACAGTCATCCAAAGCCCAAGTTGCAGCACAGTCTCAGCCTCAAAGTA ATGTCCAAGGACAGNTCTCTGTTCGTGTCCCAAGTCCATCACAGACTCGAATACGTCCAT CAACTNCTN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL833375 unedited GTACAGCTATGNNACCGCGGCCGCAATCTAGNGATCGGTTTTTTTTTTTTTTTTTTAATA AACAAAGTTTTCTCGCTTCTGCCACAATAGTAAAACCATCTGATCTTGACAAGATAATGG TGTCGTTGACTTTGCTTTTTTCTTGTCCGTTGGACAAAATTGGCCAAGAATATAATTGGA CTGTTATGACCAATAAAAACGAAGTTTAGGTCAAGTCTTGTCAGGATAGCCTGACTAAAA ACATCTGGCTCCTTAATTTAAAATAGTTCAGACAACCAGATTCTTGCTGTGTTTTATGTT AGGTTAACACGCTGAACTTTAAGAAGCTGTAGACTGCAGTTTGTTGTTATGAGACCTGCT AGCTTTGAAGCCTTTCAATTTCTGTACAAAGAATGATTCGAGAACTTCTGCACACTGGTA AAATGGGGAGTCACTTGGATTGTAGTAACGACAGTTATCAAAAATTTTGGTCATATCTGC CACAAATTCCGTCAGCTTTTCATAATATCGTCTTTGTACTCTTTCTTCCATGGTGGCAAG GTCCATAGGTTCCTTAATAACACCATAATAATCTGGTGCATCATTAGGGTCTACTGGTTC AAGGAAAGGCCAGGCCATCTTATGGGCCTGTAAGGAACGGAGCACCCTCTTCAACCCCTC ATAATCCTTCTCTGTTAGTGGCGTGAGCACTGTCATGGCATCCTCTGTTGACTGGCACTG TGGACAGACATACTCATCAATGAGCTCTGCCTCACTTTGCAAGATGCCAACGCAGCGCCC ATGGTACCAATTCTGACACCGATCACAGCCAATATAAAATTGTGACTCATCATAAGGGTG TTCTGCAGATACAGTACAATTCCTCACTGCTGCCCTCTTGTGCCCGTTTACATCATACAG ATGTACACATCCATTTTCTTAGCCTNCTTTTCTGTGATGCCA
Restriction Sites	NotI-NotI
ACCN	AL833375
Insert Size	2850 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	AL833375.1
RefSeq Size	4074 bp
RefSeq ORF	4074 bp
Locus ID	2186
Domains	BROMO, PHD
Protein Families	Druggable Genome
Gene Summary	This gene was identified by the reactivity of its encoded protein to a monoclonal antibody prepared against brain homogenates from patients with Alzheimer's disease. Analysis of the original protein (fetal Alz-50 reactive clone 1, or FAC1), identified as an 810 aa protein containing a DNA-binding domain and a zinc finger motif, suggested it might play a role in the regulation of transcription. High levels of FAC1 were detected in fetal brain and in patients with neurodegenerative diseases. The protein encoded by this gene is actually much larger than originally thought, and it also contains a C-terminal bromodomain characteristic of proteins that regulate transcription during proliferation. The encoded protein is highly similar to the largest subunit of the Drosophila NURF (nucleosome remodeling factor) complex. In Drosophila, the NURF complex, which catalyzes nucleosome sliding on DNA and interacts with sequence-specific transcription factors, is necessary for the chromatin remodeling required for transcription. Two alternative transcripts encoding different isoforms have been described completely. [provided by RefSeq, Jul 2008]

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