DARS2 (AL833633) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASPRS; LBSL; MT-ASPRS
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AL833633, the custom clone sequence may differ by one or more nucleotides GGCAGACTGATGCTTTAAGACTCAGGGAGAGGTCTTTCCCTTATCTCCACCCCAGCAAGCACCCCAGAGA CCTTGGAGATTTGTCTTGTTTCTAGACACGTGTACTCCAATGTTGTGCGGAGGAGGCCTTAAATATTCGA GAAGAGAGTGGGAACTCCTGGAATTTTAAGGGATTTCTGTGTATTTCCAAAACTGACTTTTAACTACCGG CAGCGTGGGGTTTCGTGATTGTTTTTCGCCATCGTGTGGCTCCAACATGTACTTCCCTTCTTGGTTAAGT CAGCTGTACAGGGGTTTATCCAGACCCATCAGAAGGACCACCCAACCGATCTGGGGTTCTCTCTACAGAA GTCTGTTGCAGAGTTCACAGAGGAGAATTCCAGAATTCAGTAGCTTTGTTGTCCGGACCAACACATGTGG AGAGTTGCGTTCGTCTCACTTAGGCCAAGAAGTCACCTTGTGTGGATGGATTCAGTACCGAAGGCAAAAC ACATTCTTGGTCCTAAGAGATTTCGATGGGCTTGTTCAAGTTATCATTCCCCAGGATGAGTCGGCAGCCT CTGTGAAGAAGATTTTATGTGAAGCCCCTGTGGAATCTGTGGTGCAAGTGTCTGGTACAGTCATTTCCCG TCCTGCAGGACAAGAGAATCCAAAAATGCCAACAGGTGAGATTGAAATCAAAGTTAAAACAGCTGAGCTT CTGAATGCCTGCAAGAAGCTGCCCTTTGAAATTAAGAACTTCGTGAAGAAAACAGAGGCTCTTCGGTTGC AGTATCGCTACTTAGACTTGCGTAGTTTCCAAATGCAGTATAACCTGCGACTGAGGTCCCAGATGGTCAT GAAAATGCGGGAATATCTCTGTAATCTGCATGGGTTTGTGGATATAGAAACCCCCACATTGTTTAAGAGG ACCCCAGGGGGTGCCAAAGAGTTTTTAGTACCATCCAGGGAACCTGGAAAGTTTTATTCTCTCCCTCAGA GTCCTCAACAGTTTAAGCAACTTCTGATGGTTGGCGGTTTAGACAGATATTTTCAGGTTGCCCGATGTTA TCGAGATGAAGGTTCAAGACCAGACAGACAGCCTGAGTTTACTCAGATTGACATAGAGATGTCATTTGTA GACCAGACTGGGATCCAGAGTTTAATTGAGGGTTTGCTCCAGTATTCCTGGCCCAATGACAAAGATCCTG TGGTTGTTCCTTTTCCTACTATGACTTTTGCTGAGGTGCTGGCCACCTATGGAACTGATAAACCTGACAC TCGCTTTGGAATGAAGATTATAGATATCAGTGATGTGTTTAGAAACACAGAGATTGGATTTCTTCAAGAT GCACTTAGTAAGCCCCATGGAACTGTGAAAGCCATATGTATCCCTGAAGGAGCAAAATACTTAAAAAGGA AAGACATTGAATCCATTAGAAACTTTGCAGCTGACCATTTTAATCAGGAAATCTTACCTGTATTCCTTAA CGCCAATAGAAACTGGAATTCTCCAGTTGCTAATTTCATAATGGAGTCACAAAGACTGGAATTAATCAGA CTAATGGAGACCCAAGAGGAAGATGTGGTCCTACTAACTGCTGGAGAGCACAATAAAGCATGCTCTTTGT TAGGAAAATTACGACTGGAATGTGCTGACCTTCTAGAAACAAGAGGAGTGGTGCTCCGTGACCCCACTCT GTTCTCTTTCCTTTGGGTGGTAGATTTCCCACTCTTCCTGCCCAAGGAGGAAAATCCCAGAGAGCTGGAA TCGGCCCACCACCCATTTACTGCTCCCCACCCCAGTGACATACATCTCCTGTACACTGAGCCCAAAAAGG CCCGTAGCCAACACTATGACTTGGTTTTAAATGGCAATGAAATAGGAGGTGGTTCAATTCGAATTCACAA TGCAGAGCTGCAGCGTTATATCCTGGCAACCTTACTAAAGGAGGATGTGAAAATGCTCTCCCATCTGCTC CAGGCTTTAGATTATGGGGCACCCCCTCATGGAGGAATTGCCTTAGGGTTAGACAGACTGATATGCCTTG TCACTGGATCTCCAAGCATCAGAGATGTCATAGCCTTCCCAAAGTCCTTCCGGGGACATGACCTCATGAG CAATACCCCAGATTCTGTCCCTCCTGAGGAACTGAAGCCCTATCATATCCGAGTCTCCAAGCCAACAGAC TCCAAAGCAGAAAGAGCTCATTGAATCATGCATACCATGCAGAAAGTTGAGCTTTTAGGTTTTGTCCTCT TTGCTTCCCCAAGGCTAAAGTCAGATCTAGAGTTCTGCCACAGGTCTAACAATCAAGTCTTTAGATGGAA GGAATCCAGGCAACATTCTTCACCACAACGAAGAAACAGATAAAAGATACCCAATTTTGACTTGAAAAAA AAAAAAAAAAA 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AL833633 unedited CGGATTTGTAATCGNCTTTACTATAGGCGGCCGCGCAATTCGCACGAGGCCTCGTGCCGA ATTCGGCACGAGGGTGGGATTTCGTGATTGTTTTTCGCCATCGTGTGGCTCCAACATGTA CTTCCCTTCTTGGTTAAGTCAGCTGTACAGGGGTTTATCCAGACCCATCAGAAGGACCAC CCAACCGATCTGGGGTTCTCTCTACAGAAGTCTGTTGCAGAGTTCACAGAGGAGAATTCC AGAATTCAGTAGCTTTGTTGTCCGGACCAACACATGTGGAGAGTTGCGTTCGTCTCACTT AGGCCAAGAAGTCACCTTGTGTGGATGGATTCAGTACCGAAGGCAAAACACATTCTTGGT CCTAAGAGATTTCGATGGGCTTGTTCAAGTTATCATTCCCCAGGATGAGTCGGCAGCCTC TGTGAAGAAGATTTTATGTGAAGCCCCTGTGGAATCTGTGGTGCAAGTGTCTGGTACAGT CATTTCCCGTCCTGCANGACAAGAGAATCCAAAAATGCCAACAGGTGAGATTGAAATCAA AGTTAAAACAGCTGAGCTTCTGAATGCCTGCAAGAAGCTGCCCCTTGAAATTAAGAAACT CGTGAAAGAAACAGAGGCTCTTCNGTTGCAGTATCGCTACTTAGACTTGCGTAGTTTCAA ATGCAGTATAACCTGCGACTGAGGTCCCAGATGTTCATGAAAATGCGGGAATATCTCTGT AATCTGCATGGGTTTGTGGGATATAGGACCCCCCCCATGGTTAAGAAGACCCCAGGGGTT GCCAAGAGTTTTTAGTACATTCCAGGGAACCTGAAAAGTTTATTCTCTTCCTCAAAATCT TAAAAAGTTAAGCACCTCTTGATGGTTGGCGTTTAAAACAAATTTTTCAGGTTGCCCGAT GTTTTCAGAATAAAGTTCAAAACCCACAAAACGCCTGGGTTTCCCCAATTGACTAAAAGT TACTTTT 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AL833633 unedited CGTACTTTATGNACCCGGCCGCATTCTATGATCGGTTTTTTTTTTTTTTTTTTGGGTTTT CAAAGTACTTTTATTCACTTTATCTTCCATATATGTTATCTCCTCTGATTCCAGCAATAA CAGCCCGGTGAGGTAGCCAGGGCAAGTATGTATTTTACACATTAGCAGGAAGGGAGGCTA AGCGAGGTTTATGTAACTTACTCAGGCTGAAACACTGAAGAAAAATTTGTGACTCTCATT TCAGTGATGTTTTCTGCATTATTAAAAAATATTATGCTACTCCTCACTATATTATGTTGA TGGTTGAAATGTCATTATAAAGCTTAATTTATATGATTCTCTTGATGAGGATGATGAAGC AAATGCTCCATCAACTCACTAGTTTACAGGGGCAAGCATTTTCCTACATTTCACACATAA TTTGATTACCTCTGTCCTAAGTGAATAATCTACTATCTGGGTATGAGAAACATGATTTGA AAACACTAAACCACTATATTATTTCAACAAAGAACCATCTTTCACACCTAAGTAAAAAGG AACTTCACAAAAAGTCCTCACCAAAAAAAATCCAAATGATGCCTGAAATCAAGTCAAAAT TGGGGATCTTTTATCTGGTTCCTTCGTGTGGAGAAGAATGTTGCCTGGATCCCTCCATCT AAAGACTTGATTGGTAGACCTGTGGGAGAACTCTAGATCTGACCTTAACCTTGGGGGAAC AAAGGAGACAAAACCTAAAAGCTCCACCTTCTGCCTGGGATGCCTGATTCAATGAGCTCC TTTTTGCTTTGAAGCTCGTGGGCTTGGAAACCTCGTAATTATAGGGCTTCAGTTCCTCCA GAGGCCAGAACTTGGGGGTTTGGTCATGAGGGCATGTCCCCGGGAAGACTTTGGGAAGGC T
Restriction Sites	NotI-NotI
ACCN	AL833633
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AL833633.1
RefSeq Size	2391 bp
RefSeq ORF	2391 bp
Locus ID	55157
Domains	GAD, tRNA-synt_2, tRNA_anti
Protein Pathways	Aminoacyl-tRNA biosynthesis
Gene Summary	The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009]

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