HLA (BC008403) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DRB1; DRw10; HLA-DR1B; HLA-DRB; SS1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >SC106319 representing BC008403. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCGGCCGCGAATTCGGCACGAGGCTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATG GTGTGTCTGAGGCTCCCTGGAGGCTCCTGCATGGCAGTTCTGACAGTGACACTGATGGTGCTGAGCTCC CCACTGGCTTTGGCTGGGGACACCAGACCACGTTTCTTGGAGTACTCTACGTCTGAGTGTCATTTCTTC AATGGGACGGAGCGGGTGCGGTTCCTGGACAGATACTTCTATAACCAAGAGGAGTACGTGCGCTTCGAC AGCGACGTGGGGGAGTTCCGGGCGGTGACGGAGCTGGGGCGGCCTGATGAGGAGTACTGGAACAGCCAG AAGGACATCCTGGAAGACGAGCGGGCCGCGGTGGACACCTACTGCAGACACAACTACGGGGTTGTGGAG AGCTTCACAGTGCAGCGGCGAGTCCATCCTAAGGTGACTGTGTATCCTTCAAAGACCCAGCCCCTGCAG CACCACAACCTCCTGGTCTGTTCTGTGAGTGGTTTCTATCCAGGCAGCATTGAAGTCAGGTGGTTCCGG AATGGCCAGGAAGAGAAGACTGGGGTGGTGTCCACAGGCCTGATCCACAATGGAGACTGGACCTTCCAG ACCCTGGTGATGCTGGAAACAGTTCCTCGGAGTGGAGAGGTTTACACCTGCCAAGTGGAGCACCCAAGC GTGACAAGCCCTCTCACAGTGGAATGGAGAGCACGGTCTGAATCTGCACAGAGCAAGATGCTGAGTGGA GTCGGGGGCTTTGTGCTGGGCCTGCTCTTCCTTGGGGCCGGGCTGTTCATCTACTTCAGGAATCAGAAA GGACACTCTGGACTTCAGCCAAGAGGATTCCTGAGCTGAAGTGCAGATGACACATTCAAAGAAGAACTT TCTGCCCCAGCTTTGCAGGATGAAAAGCTTTCCCTCCTGGCTGTTATTCTTCCACAAGAGAGGGCTTTC TCAGGACCTGGTTGCTACTGGTTCAGCAACTGCAGAAAATGTCCTCCCTTGTGGCTTCCTCAGCTCCTG TTCTTGGCCTGAAGCCCACAGCTTTGATGGCAGCGCCTCATCTTCAACTTTTGTGCTCCCCTTTGCCTA AACCCTATGGCCTCCTGTGCATCTGTACTCACCCTGTACCACAAACACATTACATTATTAAATGTTTCT CAAAGATGGAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGC 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for BC008403 unedited AGGAATTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGCTTGCCTGC TGCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCTGAGGCTCCCTGGAGG CTCCTGCATGGCAGTTCTGACAGTGACACTGATGGTGCTGAGCTCCCCACTGGCTTTGGC TGGGGACACCAGACCACGTTTCTTGGAGTACTCTACGTCTGAGTGTCATTTCTTCAATGG GACGGAGCGGGTGCGGTTCCTGGACAGATACTTCTATAACCAAGAGGAGTACGTGCGCTT CGACAGCGACGTGGGGGAGTTCCGGGCGGTGACGGAGCTGGGGCGGCCTGATGAGGAGTA CTGGAACAGCCAGAAGGACATCCTGGAAGACGAGCGGGCCGCGGTGGACACCTACTGCAG ACACAACTACGGGGTTGTGGAGAGCTTCACAGTGCAGCGGCGAGTCCATCCTAAGGTGAC TGTGTATCCTTCAAAGACCCAGCCCCTGCAGCACCACAACCTCCTGGTCTGTTCTGTGAG TGGTTTCTATCCAGGCAGCATTGAAGTCAGGTGGTTCCGGAATGGCCAGGAAGAGAAGAA CTGGGTGGTGTCCACAGGCCTGATCCACAATGGAGACTGGACCTTCCAGACCCTGGTGAT GCTGGAAACAGTTCCTCGGAGTGGAGAGGTTTACACCTGCCAAGTGGAGCACCCAAGCGT GACAAGCCCTCTCACAGTGGAATGGAGAGCACGGGTCTGATCTGCACNAGCAAGTGCTGA GTGGAGTCGGGGGCTTTGTGCTGGGCCTGCTCTTCCTTGGGCCNGGGCTGTTCATCTACT TCAGGATCAGAAAGGACACTCTGNACTTCANCCCAGAGGATTCCTGANCTGAN
Restriction Sites	NotI-NotI
ACCN	BC008403
Insert Size	1221 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC008403.1
RefSeq Size	1202 bp
RefSeq ORF	1221 bp
Locus ID	3123
Domains	MHC_II_beta, ig, IGc1
Protein Families	Transmembrane
Protein Pathways	Allograft rejection, Antigen processing and presentation, Asthma, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Graft-versus-host disease, Hematopoietic cell lineage, Systemic lupus erythematosus, Type I diabetes mellitus, Viral myocarditis
MW	45.7 kDa
Gene Summary	HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and some alleles have increased frequencies associated with certain diseases or conditions. For example, DRB1*1302 has been related to acute and chronic hepatitis B virus persistence. There are multiple pseudogenes of this gene. [provided by RefSeq, Jul 2020]

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