United States
Germany
Japan
United Kingdom
China
PTBP1 (NM_031990) Human Untagged Clone
CAT＃: SC106769
PTBP1 (untagged)-Human polypyrimidine tract binding protein 1 (PTBP1), transcript variant 2
CNY 4,104.00
CNY 8,840.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HNRNP-I; HNRNPI; HNRPI; pPTB; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC106769 sequence for NM_031990 edited (data generated by NextGen Sequencing)
ATGGACGGCATTGTCCCAGATATAGCCGTTGGTACAAAGCGGGGATCTGACGAGCTTTTC TCTACTTGTGTCACTAACGGACCGTTTATCATGAGCAGCAACTCGGCTTCTGCAGCAAAC GGAAATGACAGCAAGAAGTTCAAAGGTGACAGCCGAAGTGCAGGCGTCCCCTCTAGAGTG ATCCACATCCGGAAGCTCCCCATCGACGTCACGGAGGGGGAAGTCATCTCCCTGGGGCTG CCCTTTGGGAAGGTCACCAACCTCCTGATGCTGAAGGGGAAAAACCAGGCCTTCATCGAG ATGAACACGGAGGAGGCTGCCAACACCATGGTGAACTACTACACCTCGGTGACCCCTGTG CTGCGCGGCCAGCCCATCTACATCCAGTTCTCCAACCACAAGGAGCTGAAGACCGACAGC TCTCCCAACCAGGCGCGGGCCCAGGCGGCCCTGCAGGCGGTGAACTCGGTCCAGTCGGGG AACCTGGCCTTGGCTGCCTCGGCGGCGGCCGTGGACGCAGGGATGGCGATGGCCGGGCAG AGCCCCGTGCTCAGGATCATCGTGGAGAACCTCTTCTACCCTGTGACCCTGGATGTGCTG CACCAGATTTTCTCCAAGTTCGGCACAGTGTTGAAGATCATCACCTTCACCAAGAACAAC CAGTTCCAGGCCCTGCTGCAGTATGCGGACCCCGTGAGCGCCCAGCACGCCAAGCTGTCG CTGGACGGGCAGAACATCTACAACGCCTGCTGCACGCTGCGCATCGACTTTTCCAAGCTC ACCAGCCTCAACGTCAAGTACAACAATGACAAGAGCCGTGACTACACACGCCCAGACCTG CCTTCCGGGGACAGCCAGCCCTCGCTGGACCAGACCATGGCCGCGGCCTTCGCCTCTCCG TATGCAGGAGCTGGTTTCCCTCCCACCTTTGCCATTCCTCAAGCTGCAGGCCTTTCCGTT CCGAACGTCCACGGCGCCCTGGCCCCCCTGGCCATCCCCTCGGCGGCGGCGGCAGCTGCG GCGGCAGGTCGGATCGCCATCCCGGGCCTGGCGGGGGCAGGAAATTCTGTATTGCTGGTC AGCAACCTCAACCCAGAGAGAGTCACACCCCAAAGCCTCTTTATTCTTTTCGGCGTCTAC GGTGACGTGCAGCGCGTGAAGATCCTGTTCAATAAGAAGGAGAACGCCCTAGTGCAGATG GCGGACGGCAACCAGGCCCAGCTGGCCATGAGCCACCTGAACGGGCACAAGCTGCACGGG AAGCCCATCCGCATCACGCTCTCGAAGCACCAGAACGTGCAGCTGCCCCGCGAGGGCCAG GAGGACCAGGGCCTGACCAAGGACTACGGCAACTCACCCCTGCACCGCTTCAAGAAGCCG GGCTCCAAGAACTTCCAGAACATATTCCCGCCCTCGGCCACGCTGCACCTCTCCAACATC CCGCCCTCAGTCTCCGAGGAGGATCTCAAGGTCCTGTTTTCCAGCAATGGGGGCGTCGTC AAAGGATTCAAGTTCTTCCAGAAGGACCGCAAGATGGCACTGATCCAGATGGGCTCCGTG GAGGAGGCGGTCCAGGCCCTCATTGACCTGCACAACCACGACCTCGGGGAGAACCACCAC CTGCGGGTCTCCTTCTCCAAGTCCACCATCTAG Clone variation with respect to NM_031990.3 >OriGene 5' read for NM_031990 unedited
TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCACTCCGTCC CCCGCGGGTCTGCTCTGTGTGCCATGGACGGCATTGTCCCAGATATAGCCGTTGGTACAA AGCGGGGATCTGACGAGCTTTTCTCTACTTGTGTCACTAACGGACCGTTTATCATGAGCA GCAACTCGGCTTCTGCAGCAAACGGAAATGACAGCAAGAAGTTCAAAGGTGACAGCCGAA GTGCAGGCGTCCCCTCTAGAGTGATCCACATCCGGAAGCTCCCCATCGACGTCACGGAGG GGGAAGTCATCTCCCTGGGGCTGCCCTTTGGGAAGGTCACCAACCTCCTGATGCTGAAGG GGAAAAACCAGGCCTTCATCGAGATGAACACGGAGGAGGCTGCCAACACCATGGTGAACT ACTACACCTCGGTGACCCCTGTGCTGCGCGGCCAGCCCATCTACATCCAGTTCTCCAACC ACAAGGAGCTGAAGACCGACAGCTCTCCCAACCAGGCGCGGGCCCAGGCGGCCCTGCAGG CGGTGAACTCGGTCCAGTCGGGGAACCTGGCCTTGGCTGCCTCGGCGGCGGCCGTGGACG CAGGGATGGCGATGGCCGGGCAGAGCCCCGTGCTCAGGATCATCGTGGAGAACCTCTTCT ACCCTGTGACCCTGGATGTGCTGCACCAAATTNTCTCCAAGTTCGGCACAGTGTTGAAGA TCATCACCTTCACCAAGAAACACCAGTTCCAGGCCCCTGCTGCAGTATGCGGACCCCGTG AACCGCCCAACACGCAAGCTGGTCCCTGGACGGGCAGACATCTACAACGCCTGGTGCCCG CTGGGCATCGACTTTTCAAGCTCACCGGCTCACGTCAAGTACACCATGACAAGAGCGGGA CTACCACCCCAGACCTG >OriGene 3' read for NM_031990 unedited
GCCCGTTTCTATATCGAGTTTTTTTTTTTTTTTTTTGGAGTTTCTGAAACGGTTTTAATC GGTTCTCTCCGCGTCACAAGCCATCGGGTAAGGCAACGGTAATGTGCGTGGGGTCCCCTG TGGCTTCGTGGTTACAATACTGAGCCTGGAATTGCTGTTAGCAAAATATACATTTGTGTC ACCATAAAAAACCGCGCCGTCGTCCCTCGGGTCTTACAACAGGTATAAAAAATTATAAAT ATTTACACCCTTGTTACACGCTTTTACGGAAAGGGGATCCTAAGATAGCCCCCGGGACAG GACTCGGGGGCGGCACAAAGAGCACAATGACAGGATCGCCCGTCTTTCGGGTTCATCCTC CTAGGCAGGTTTAACACAGAAATGAGACTTTGGCGCAAAATTTGAAGGAAGAAAAAACCC TGAAGTTTGAGAGCAAGTTTTTTATGCGACTGCTGGTGAAAGCGTGGTGCAATGCTACCG GAGGCGATGTCAGAGGCGGTGACACCTTCGCCCCTTACCTGAAGCTTAAGGGCTCTAACA TAGGACCGGGGCTACCTGTGTCTGACCCATGGGCGGTCCCGTCTGCTCTCTGGAACCTGG TTAGGACCGGGCTTTCTGTGCCTGCCCACTGGGCGGTCCCGCCTGCTTTCTGGAAACTGG GGTCTGCCTGTGCCTTATTTCCAGGAATAAATACAGCTACAGCGTCCAGGGCATACCCCT GGAAATTTTATTTCCCTCCACCCTTGAGTCTCACTGCATAAAAGATACTAAAATATTCCT TTTGCTTTAATGGATAAGATTCCATATCTGGCTACCGGCAAATTGCTAGGCCACACCTTG CTCTGCTAGTTTTATTTAGATATTTTTCTTCCCTCCCTACTGAAACCCGGGACCCATTGG GTGCATAGATAGCCCGGGGCGGGAAAGGAATTCCGTCTGTTAAGGCGGGCGGGGTTCTCC CC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_031990 |
| Insert Size | 3070 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_031990.2, NP_114367.1 |
| RefSeq Size | 3300 bp |
| RefSeq ORF | 1653 bp |
| Locus ID | 5725 |
| UniProt ID | P26599 |
| Domains | RRM |
| Protein Families | Druggable Genome |
| Gene Summary | This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternative splice site resulting in it lacking 21 nt in the coding region compared to variant 1. Thus isoform b is 7 aa shorter than isoform a, but it maintains the same reading frame. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217785 | PTBP1 (Myc-DDK-tagged)-Human polypyrimidine tract binding protein 1 (PTBP1), transcript variant 2 |
CNY 4,096.00 |
|
| RC217785L3 | Lenti ORF clone of Human polypyrimidine tract binding protein 1 (PTBP1), transcript variant 2, Myc-DDK-tagged |
CNY 6,650.00 |
|
| RC217785L4 | Lenti ORF clone of Human polypyrimidine tract binding protein 1 (PTBP1), transcript variant 2, mGFP tagged |
CNY 6,650.00 |
|
| RG217785 | PTBP1 (tGFP-tagged) - Human polypyrimidine tract binding protein 1 (PTBP1), transcript variant 2 |
CNY 5,230.00 |
