E2F6 (NM_198257) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	E2F-6; E2F-6, MGC111545; E2F transcription factor 6; E2F transcription factor 6, isoform 1; MGC111545
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC107802 sequence for NM_198257 edited (data generated by NextGen Sequencing) ATGAGTCAGCAGCGGCCGGCGAGGAAGTTACCCAGTCTCCTCCTGGACCCGACGGAGGAG ACGGTTCGCCGTCGGTGCCGAGACCCCATCAACGTGGAGGGCCTGCTGAAGCTCTAA Clone variation with respect to NM_198257.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_198257 unedited NGTTCGAATTTGTATACGACTCACTATAGGCGGCCCGCGATTCGGCACGAGGGAGAGGCG GGCCGGGCGACTTGAGATCCGCGGGTCTCCCTCCTCTTTTTCCGTCTGCGTCGGGAGCTC CCGGGCACGTGAGGCCGTGCCGCGTTTACTGGCGGGCGGGACGGCCTAGCCGGGCGGCGC CTCGGAGGAAGCCGCGGACCCCTTAGGTGCTGGGCCCTTGGAAATCGGCGCGTGGGGGGC GGTGCTCGAGCTGAGCGCGAGAGGGCGGGAGAGCTCGTGGGGTGCGAGGGGAGCAGGACG CCCGGCCGGGCAGCATGAGTCAGCAGCGGCCGGCGAGGAAGTTACCCAGTCTCCTCCTGG ACCCGACGGAGGAGACGGTTCGCCGTCGGTGCCGAGACCCCATCAACGTGGAGGGCCTGC TGAAGCTCTAAAAGTGAAGAGACCTCGTTTTGATGTATCGCTGGTTTATTTAACTCGAAA ATTTATGGATCTTGTCAGATCTGCTCCCGGGGGTATTCTTGACTTAAACAAGGTTGCAAC GAAACTGGGAGTCCGAAAGCGGAGAGTGTATGACATCACCAATGTCTTAGATGGAATCGA CCCTCGTTGAAAAGAAATCCAAGAACCATATTACATGGATAGGATCTGATCTTAGCAATT TTGGAGCAGTTCCCCACCAAAAGAAGCTACAGGAGGAACTTTCTGACTTATCAGCAATGG AAGATGCTTTGGATGAACTAATTAACGATTGTGCTCACCAGCTGTTTGAGTTACCAGATG ACCCAGCACAGGAAAGACTACTTATGTGACCTATCCAGACATTCTAGCCTTTANGCCTTC CATGAACAGATCGCCCTTTGCAAGTCAAGCTCCAGCCGAACCAGACTTGGGTGGTCCCAG CTCCCACAACAA 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_198257 unedited CTAGAGTCGAGTTTTTTTTTTTTTTTTTTGTTCAGACATTTATTGAGCACTTCTTAAGGG CCAGGCATGGTGTGAGGCACTGGGATGTAACAGTGATTATGACAAAGTCCCTCAAGGAGC TCACAGTCTAATGGTAAACTACAGAGTAAACTGAGGCACAGAGTGGTAAATGACTTACCC AATGAGGTAGAAACAGCTAGGTATGTGAAAATGTGGGAGCAGAGCTGGAGAGAGGGCAAG GTACAACTTGTCCTAAGTAAAACCCCTTGGACTGACTCAACAGCAAGGCTGCATACACAA ACATTACAAAATCTACTTGCAAAAACCTGAAAGGAGTTTCCAATTATCACCATGACTGAA TTCATGTTTCACAATGAATTCAGCTATTTCCTGATGGCATTTCTATCCTTGCCCCCAAAG CCACTGGGCATGCCACCAGTCTGCTGGGAGACAGCAATTAGGTAGACAGCTTAAAACCAA GAAGTATCTGACTCCAATTTTAGCACTGAAAACCATATTAAAATACTTTAATTAAATCCA CAAACATAAATGTGTAGCACATTTATGATACTTCTACATGTAGAAGTTTGGAAAAAGTCT CTTCTACCCTCATCCAGAGATTCTAATAAGTTTAGATACCAAGAGATAGAGAATTGTCTG CACTTTCATAATACATATTTTGCTGTCANGGGGAAAATGTACCTAAACTATTTCGAGTAT CTCACCTTTCCGATTATCCAAACTCCCTTTAATCCTGTGACCTTTTGCAAGTACAAAGTA CTGCAACGTGAACAGGGAAAGAATTCTTTGGGAAATGACATCCCCTGAGGAAGGTAAGCC CAAGAACGGCTTCCACCGACGTTTGTTCTAACTTGGAGAACTTTTAGGGCAATTCTTTCC TGACAACCTGATATTTAAGGGTTTTTATGGAACCCTTCTTATTTAGGGATCCAAAACTCG CCGAGCG
Restriction Sites	NotI-NotI
ACCN	NM_198257
Insert Size	2380 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_198257.1, NP_937988.1
RefSeq Size	2287 bp
RefSeq ORF	117 bp
Locus ID	1876
Protein Families	Transcription Factors
Gene Summary	This gene encodes a member of a family of transcription factors that play a crucial role in the control of the cell cycle. The protein encoded by this gene lacks the transactivation and tumor suppressor protein association domains found in other family members, and contains a modular suppression domain that functions in the inhibition of transcription. It interacts in a complex with chromatin modifying factors. There are pseudogenes for this gene on chromosomes 22 and X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] Transcript Variant: This variant (e) lacks an alternate exon, compared to variant a, that causes a frameshift. The predicted isoform (3) is shorter at the N-terminus compared to isoform 1. Variants c, d, and e all encode isoform 3.

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