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BRF1 (NM_145685) Human Untagged Clone
CAT＃: SC108221
BRF1 (untagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3
CNY 8,320.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BRF; BRF-1; CFDS; GTF3B; hBRF; HEL-S-76p; TAF3B2; TAF3C; TAFIII90; TF3B90; TFIIIB90 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_145685, the custom clone sequence may differ by one or more nucleotides
ATGACTGCCCTGAGGCTCCTACAGAGGATGAAGCGGGACTGGATGCACACAGGCCGGCGCCCCTCGGGCC TCTGCGGAGCAGCGCTCCTGGTTGCAGCCAGAATGCATGACTTCAGGAGGACTGTGAAGGAGGTCATCAG TGTGGTCAAAGTGTGTGAGTCCACGCTGCGGAAGAGGCTCACGGAATTTGAAGACACCCCCACCAGTCAG TTGACCATTGATGAGTTCATGAAGATCGACCTGGAGGAGGAGTGCGACCCCCCCTCGTACACAGCTGGGC AGAGGAAGCTGCGGATGAAGCAGCTTGAACAAGTCCTGTCAAAAAAACTGGAGGAGGTTGAAGGTGAAAT ATCCAGTTACCAGGATGCAATTGAGATTGAACTAGAAAACAGCCGGCCAAAGGCCAAGGGGGGCCTGGCC AGCCTGGCAAAAGATGGCTCCACCGAGGACACCGCGTCCAGCTTGTGTGGCGAGGAGGACACAGAGGACG AGGAGCTGGAAGCCGCGGCCAGCCACCTGAACAAAGACTTATACCGGGAGCTCCTTGGTGGTGCCCCCGG CAGCTCGGAAGCAGCAGGAAGCCCCGAGTGGGGCGGCAGACCTCCGGCCCTGGGGTCCCTGCTGGACCCC CTCCCCACTGCAGCCAGCCTGGGCATCTCAGACTCCATCCGCGAATGCATCTCCTCTCAGAGCAGCGACC CCAAAGATGCTTCAGGAGACGGTGAGCTGGACCTCAGTGGCATTGATGACCTGGAGATTGACAGGTACAT CCTGAATGAGTCGGAAGCCCGCGTGAAGGCCGAGCTGTGGATGAGGGAGAACGCCGAGTACCTGCGGGAA CAGAGGGAAAAAGAAGCAAGAATAGCGAAAGAGAAGGAGCTCGGCATCTACAAGGAACACAAGCCCAAGA AGTCTTGCAAGCGACGGGAGCCAATTCAGGCCAGTACCGCCAGGGAGGCCATCGAGAAGATGCTGGAGCA GAAGAAGATCTCCAGCAAGATCAATTATAGCGTGCTCCGGGGCCTCAGCAGCGCCGGCGGGGGCAGTCCG CACAGGGAGGATGCACAGCCCGAGCATAGCGCCAGTGCCAGGAAGCTGTCACGAAGGAGGACGCCGGCCA GCAGAAGTGGGGCTGACCCTGTGACCAGTGTGGGGAAAAGGTTGAGGCCTCTGGTGTCTACGCAGCCAGC AAAGAAGGTGGCCACGGGAGAGGCTTTGCTCCCAAGCTCTCCCACCCTCGGAGCTGAGCCTGCCAGGCCC CAGGCGGTGCTGGTGGAGAGCGGGCCCGTGTCATACCACGCCGACGAGGAGGCTGACGAGGAGGAGCCTG ACGAGGAGGACGGGGAGCCCTGCGTCAGTGCCCTGCAGATGATGGGCAGCAACGACTATGGCTGTGATGG CGATGAGGACGACGGCTACTGA >OriGene 5' read for NM_145685 unedited
TATTCCCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGC AGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCG AATTCGGCACGAGGGAAAGACGTTTCTTCTCTTGGCAAGAGAGCTCTGCATCAATGCGCC GGCCATAGACCCGTGCCTGTATATTCCACGCTTTGCGCACCTGCTGGAATTCGGGGAGAA GAACCACGAGGTGTCCATGACTGCCCTGAGGCTCCTACAGAGGATGAAGCGGGACTGGAT GCACACAGGCCGGCGCCCCTCGGGCCTCTGCGGAGCAGCGCTCCTGGTTGCAGCCAGAAT GCATGACTTCAGGAGGACTGTGAAGGAGGTCATCAGTGTGGTCAAAGTGTGTGAGTCCAC GCTGCGGAAGAGGCTCACGGAATTTGAAGACACCCCCACCAGTCAGTTGACCATTGATGA GTTCATGAAGATCGACCTGGAGGAGGAGTGCGACCCCCCCTCGTACACAGCTGGGCAGAG GAAGCTGCGGATGAAGCAGCTTGAACAAGTCCTGTCAAAAAAACTGGAGGAGGTTGAAGG TGAAATATCCAGTTACCAGGATGCAATTGAGATTGAACTAGAAAACAGCCGGCCAAAGGC CAAGGGNGGGCCTGGCCAGCCTGGCAAAAGATGGCTCCACCGANGACACCGCGTNCAGCT TGTGTGGCGAGGAGGACACANGAGACGAGGAGCTGGAAGCCGNCGCCAGCCACCTGAAAA CAAGACTATACCNGGAGCTCTTNGNTGGTGCCCCCCGCAGCTCGGAAGCAGCAGAAGCCC CCGAGTGGGGCGGCAGACCTCCGCCCCCTGGGGTCCCCTGCTGGACCCCCTCCCCACTGC AGCCAGCCTGGGCATCTCAGACTCCATCCGCGAATGCATCTNCTCTCAA >OriGene 3' read for NM_145685 unedited
NGGTTCACTATGNACCGCGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTCTGTT AAGTAACAACAGATCTTCCTGCTTTACTAAATCTATTCTTCCCCCAAGCCCTCGAGAAGC CCTGGCAGGACCCAGGCTGGCTACTGAGCTCTGGGCGGGGGTAGGGGGGTCTGGCCTGCT GCGGGCCAAGTTGGGGCAGGACATACAGCCCATTCCATGGGGAGGATGAGGCCCCTGGGG GTCAGTGAGGCACGGCTCTGCCTCAAGGCCACCCCCTCCTAAGGACACAGGGTGAAGCCC CCTCGGCCACATCCGGGGCAGCCATGCCAGAGCTGAGACCTCCTACGAGTGGTACTGGGG CCTTCCCACGAAGAGGCCTGGGGAGGCTCTCGGGCCTCCGTCTGCCCTCCCTCCTCGATG GGGGGCCTGATCCAGCTCTGACAGGGAGCGCCACTGCCCTCTGGCTCTGTCCACGGGGAA CTCCCAGCGCCAGGACACTATGCAGGCTATGCCCGCACTGCCTACAGAGCTATGCTCAGG ACGGGTGGGCGCCGGTCTCAGCTGTCGGGCACTCAGTTCACCTGCCGGCAGCCGCAGGGC TCCTGGGCCCACAACTCAAGTGGCCCTGGAGCAAGGGTCTGACCCCCATGCTGCTCCTAG CGGTGCAAGCAACGGGGAAGGGGGTCTTGAAGAAGCAAGGTGGGGGGGTTCTTCCCCCCA CAAAAAAATATTTTGATGCCAGGCCCCCCCTGGAGGCAAAGACCCTCACAGGAGTGGCGG CTCTTAAAGGCCCAATCCTCAATTTCACAGGTGCCCCGGAAACCCGAGGGCTTTGGTTTC CCTCTCCACAAACTTTTGGCCCCAAATTTTTTAGAAGGGAACGGGCGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_145685 |
| Insert Size | 3000 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145685.1, NP_663718.1 |
| RefSeq Size | 3585 bp |
| RefSeq ORF | 3585 bp |
| Locus ID | 2972 |
| UniProt ID | Q92994 |
| Domains | transcript_fac2 |
| Protein Families | Transcription Factors |
| Gene Summary | This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant (1). The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210551 | BRF1 (Myc-DDK-tagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3 |
CNY 3,990.00 |
|
| RC210551L3 | Lenti-ORF clone of BRF1 (Myc-DDK-tagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3 |
CNY 5,890.00 |
|
| RC210551L4 | Lenti-ORF clone of BRF1 (mGFP-tagged)-Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3 |
CNY 5,890.00 |
|
| RG210551 | BRF1 (tGFP-tagged) - Human BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae) (BRF1), transcript variant 3 |
CNY 4,370.00 |
