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EED (NM_003797) Human Untagged Clone
CAT＃: SC108260
EED (untagged)-Human embryonic ectoderm development (EED), transcript variant 1
CNY 3,656.00
CNY 7,030.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | COGIS; HEED; WAIT1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC108260 sequence for NM_003797 edited (data generated by NextGen Sequencing)
ATGTCCGAGAGGGAAGTGTCGACTGCGCCGGCGGGAACAGACATGCCTGCGGCCAAGAAG CAGAAGCTGAGCAGTGACGAGAACAGCAATCCAGACCTCTCTGGAGACGAGAATGATGAC GCTGTCAGTATAGAAAGTGGTACAAACACTGAACGCCCTGATACACCTACAAACACGCCA AATGCACCTGGAAGGAAAAGTTGGGGAAAGGGAAAATGGAAGTCAAAGAAATGCAAATAT TCTTTCAAATGTGTAAATAGTCTCAAGGAAGATCATAACCAACCATTGTTTGGAGTTCAG TTTAACTGGCACAGTAAAGAAGGAGATCCATTAGTGTTTGCAACTGTAGGAAGCAACAGA GTTACCTTGTATGAATGTCATTCACAAGGAGAAATCCGGTTGTTGCAATCTTACGTGGAT GCTGATGCTGATGAAAACTTTTACACTTGTGCATGGACCTATGATAGCAATACGAGCCAT CCTCTGCTGGCTGTAGCTGGATCTAGAGGCATAATTAGGATAATAAATCCTATAACAATG CAGTGTATAAAGCACTATGTTGGCCATGGAAATGCTATCAATGAGCTGAAATTCCATCCA AGAGATCCAAATCTTCTCCTGTCAGTAAGTAAAGATCATGCTTTACGATTATGGAATATC CAGACGGACACTCTGGTGGCAATATTTGGAGGCGTAGAAGGGCACAGAGATGAAGTTCTA AGTGCTGATTATGATCTTTTGGGTGAAAAAATAATGTCCTGTGGTATGGATCATTCTCTT AAACTTTGGAGGATCAATTCAAAGAGAATGATGAATGCAATTAAGGAATCTTATGATTAT AATCCAAATAAAACTAACAGGCCATTTATTTCTCAGAAAATCCATTTTCCTGATTTTTCT ACCAGAGACATACATAGGAATTATGTTGATTGTGTGCGATGGTTAGGCGATTTGATACTT TCTAAGTCTTGTGAAAATGCCATTGTGTGCTGGAAACCTGGCAAGATGGAAGATGATATA GATAAAATTAAACCCAGTGAATCTAATGTGACTATTCTTGGGCGATTTGATTACAGCCAG TGTGACATTTGGTACATGAGGTTTTCTATGGATTTCTGGCAAAAGATGCTTGCATTGGGC AATCAAGTTGGCAAACTTTATGTTTGGGATTTAGAAGTAGAAGATCCTCATAAAGCCAAA TGTACAACACTGACTCATCATAAATGTGGTGCTGCTATTCGACAAACCAGTTTTAGCAGG GATAGCAGCATTCTTATAGCTGTTTGTGATGATGCCAGTATTTGGCGCTGGGATCGACTT CGATAA Clone variation with respect to NM_003797.3 >OriGene 5' read for NM_003797 unedited
AAACGCAATTCGCACGAGCCTCGTGCCGAATTCGGCACGAGGGTCGCACGCACGCCCGCC TCGGCGGCTGGGCGCGATTTGCGACAGTGGGGGGGGCGGTGGAGGTGGCGGCGGCAGCGG CAACTTTGCGGCAAGCTCGGGCCGGGCTTGCTTGACGGCGGTGTGGCGGAGGCCCCGCCT TATGCGGCAGGAACCTGGAGGGAGGCGGAGGAATATGTCCGAGAGGGAAGTGTCGACTGC GCCGGCGGGAACAGACATGCCTGCGGCCAAGAAGCAGAAGCTGAGCAGTGACGAGAACAG CAATCCAGACCTCTCTGGAGACGAGAATGATGACGCTGTCAGTATAGAAAGTGGTACAAA CACTGAACGCCCTGATACACCTACAAACACGCCAAATGCACCTGGAAGGAAAAGTTGGGG AAAGGGAAAATGGAAGTCAAAGAAATGCAAATATTCTTTCAAATGTGTAAATAGTCTCAA GGAAGATCATAACCAACCATTGTTTGGAGTTCAGTTTAACTGGCACAGTAAAGAAGGAGA TCCATTAGTGTTTGCAACTGTAGGAAGCAACAGAGTTACCTTGTATGAATGTCATTCACA AGGAGAAATCCGGTTGTTGCAATCTTACGTGGATGCTGATGCTGATGAAAACTTTTACAC TTGTGCATGGACCTATGATAGCAATACGAGCCATCCTCTGCTGGCTGTAGCTGGATCTAN GAGCATAATTANGATAATAAATCCTATAACAATGCAGTGTATAAAGCACTATGTTGGCCA TGGAAATGCTATCAATGAGCTGAAATTCCATCCAAGAGATCCAAATCTNCTNCTGTCAGT AAGTAAAGATTCATGCTTTACGATTATGGAATATTCAGACGGACACTCTGGC >OriGene 3' read for NM_003797 unedited
CGCGGCCGCAATCTAAAGTCGAGTTTTTTTTTTTTTTTTTTACAAAAATAAATTTTTATT AAAAGCAGTAGAGTCTGAGCAGGAAGACAGTACAAAGAATGTAAACAATGTAAACATCAC TACATTCAGCTCAGCCTGATTGAATGCTGAAAGACAACTCTAAATGCTCTACGTGCCCTT ACTAGCAAGATACATTAATTCTATTTTACACAGACAACAAACACACTCTAATTTTGATTA GGCAAAAGTATTTTATCGAAGTCGATCCCAGCGCCAAATACTGGCATCATCACAAACAGC TATAAGAATGCTGCTATCCCTGCTAAAACTGGTTTGTCGAATAGCAGCACCACATTTATG ATGAGTCAGTGTTGTACATTTGGCTTTATGAGGATCTTCTACTTCTAAATCCCAAACATA AAGTTTGCCAACTTGATTGCCCAATGCAAGCATCTTTTGCCAGAAATCCATAGAAAACCT CATGTACCAAATGTCACACTGGCTGTAATCAAATCGCCCAAGAATAGTCACATTAGATTC ACTGGGTTTAATTTTATCTATATCATCTTCCATCTTGCCAGGTTTCCAGCACACAATGGC ATTTTCACAAGACTTAGAAAGTATCAAATCGCCTAACCATCGCACACAATCAACATAATT CCTATGTATGTCTCTGGTAGAAAATCAGGAAAATGGACTTTCTGAGAAAAAATGGCCTGT TAGTNTTATTTGGGATATAATCATAAGAATCCTTAATNGCATTCATCATTCTTTTTGAAT CGATCCTCCCAGGTTTAGAAGATGATCCATCCACCGGACATTTTTTTTCCACCAAAAGAC ATAATCAGCCTTAAAACTCTCCTTGGGCCCTTTACGCTCCAAATTTGCCCCGAAGGCCGT TTGTAATTCATAACGGAAACAGAACTTCCTACTGCCGAGAAAATTGACCTTGGTGGAATT CACTATGAAGCCTCCCTGGCAAAAAGCGTTACCCGATGGTTAGATAATCCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003797 |
| Insert Size | 1690 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003797.2, NP_003788.2 |
| RefSeq Size | 2006 bp |
| RefSeq ORF | 1326 bp |
| Locus ID | 8726 |
| UniProt ID | O75530 |
| Domains | WD40 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhancer of zeste 2, the cytoplasmic tail of integrin beta7, immunodeficiency virus type 1 (HIV-1) MA protein, and histone deacetylase proteins. This protein mediates repression of gene activity through histone deacetylation, and may act as a specific regulator of integrin function. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) lacks an in-frame exon in the 3' coding region compared to variant 3. It encodes isoform a, which is shorter than isoform c. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Bromodomain protein 7 interacts with PRMT5 and PRC2, and is involved in transcriptional repression of their target genes
,Sookil Tae, Vrajesh Karkhanis, Kevin Velasco, Mariana Yaneva, Hediye Erdjument-Bromage, Paul Tempst, and Saïd Sif,
Nucleic Acids Res., Mar 2011; 10.1093/nar/gkr170
[EED]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219261 | EED (Myc-DDK-tagged)-Human embryonic ectoderm development (EED), transcript variant 1 |
CNY 3,656.00 |
|
| RC219261L1 | Lenti ORF clone of Human embryonic ectoderm development (EED), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC219261L2 | Lenti ORF clone of Human embryonic ectoderm development (EED), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC219261L3 | Lenti ORF clone of Human embryonic ectoderm development (EED), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC219261L4 | Lenti ORF clone of Human embryonic ectoderm development (EED), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG219261 | EED (tGFP-tagged) - Human embryonic ectoderm development (EED), transcript variant 1 |
CNY 5,256.00 |
