NDUFB8 (NM_005004) Human Untagged Clone
CAT#: SC108534
NDUFB8 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa (NDUFB8), nuclear gene encoding mitochondrial protein
CNY 3,600.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ASHI; CI-ASHI; MC1DN32 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_005004, the custom clone sequence may differ by one or more nucleotides
ATGGCGGTGGCCAGGGCCGGGGTCTTGGGAGTCCAGTGGCTGCAAAGGGCATCCCGGAACGTGATGCCGC TGGGCGCACGGACAGCCTCCCACATGACCAAGGACATGTTCCCGGGGCCCTATCCTAGGACCCCAGAAGA ACGGGCCGCCGCCGCCAAGAAGTATAATATGCGTGTGGAAGACTACGAACCTTACCCGGATGATGGCATG GGGTATGGCGACTACCCGAAGCTCCCTGACCGCTCACAGCATGAGAGAGATCCATGGTATAGCTGGGACC AGCCGGGCCTGAGGTTGAACTGGGGTGAACCGATGCACTGGCACCTAGACATGTACAACAGGAACCGTGT GGATACATCCCCCACACCTGTTTCTTGGCATGTCATGTGTATGCAGCTCTTCGGTTTCCTGGCTTTCATG ATATTCATGTGCTGGGTGGGGGACGTGTACCCTGTCTACCAGCCTGTGGGACCAAAGCAGTATCCTTACA ATAATCTGTACCTGGAACGAGGCGGTGATCCCTCCAAAGAACCAGAGCGGGTGGTTCACTATGAGATCTG A >OriGene 5' read for NM_005004 unedited
GCACGAGGCCTCGTGCCGAATTCGGCACGAGGGTGAAGATGGCGGTGGCCAGGGCCGGGG TCTTGGGAGTCCAGTGGCTGCAAAGGGCATCCCGGAACGTGATGCCGCTGGGCGCACGGA CAGCCTCCCACATGACCAAGGACATGTTCCCGGGGCCCTATCCTAGGACCCCAGAAGAAC GGGCCGCCGCCGCCAAGAAGTATAATATGCGTGTGGAAGACTACGAACCTTACCCGGATG ATGGCATGGGGTATGGCGACTACCCGAAGCTCCCTGACCGCTCACAGCATGAGAGAGATC CATGGTATAGCTGGGACCAGCCGGGCCTGAGGTTGAACTGGGGTGAACCGATGCACTGGC ACCTAGACATGTACAACAGGAACCGTGTGGATACATCCCCCACACCTGTTTCTTGGCATG TCATGTGTATGCAGCTCTTCGGTTTCCTGGCTTTCATGATATTCATGTGCTGGGTGGGGG ACGTGTACCCTGTCTACCAGCCTGTGGGACCAAAGCAGTATCCTTACAATAATCTGTACC TGGAACGAGGCGGGTGATCCCTCCA >OriGene 3' read for NM_005004 unedited
GCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACACAGCACTGAGTTTTAT TAGGGATTTCATTAAGGTTAAATTTCTAGGAATGAGGGAGTCCTAGTTAGAGGACCCAAA AGCCCACGAAGCCTCCTCAGATCTCATAGTGAACCACCCGCTCTGGTTCTTTGGAGGGAT CACCGCCTCGTTCCAGGTACAGATTATTGTAAGGATACTGCTTTGGTCCCACAGGCGGGT CCACAGGGTACTTGTTTTCGTCCCCCGCCCCGTGTTTCTTCACCCCTGTGCGGTCGCCCT ACTCCTCTTCCCTTTATTTATCCTCCATCCCCCCCACTCTCTTCCCCCCCCCCTTTTTTC CCCGTTCCCCATCCCTTGTTTTTGTCCAACCATTGTCCATCTACTTCCCATCCCCCGCTT ACTTTCTTCCTTCGTATTACCTTCCCCCCCCTCCATTTTTTCCCCCCCCCCCTTTCCCCT TCCTTTTCCATTCGTTTCCTTTTACCTCCACCGTTTCATTCCCCCCCCCGTTTTTCCCCC CCCCCTTCCGCCCCCCTTGCCCCCCCCCCCCGCGTTTTCCCCTTTTACCCCNTTTCCCCC CCTTTCGGTCCCCGTTCCGTTTCCTCCCTTCTCCCTTCCCCCCCTTCCCTCCGTTCTTCT GTACCCCCCCGGCTCATTTGCTTTCCTCCTTCCCCCCCTCTCCCTCCTCTCTCGCCACCT TTTCCCTTCTCCACATTTTCCTCTCAATCCCCTCGCCTTTCTCTCCCCCATTTTTGCCCC CATTTTATTGTTTTCTCCCCCCTTCCCCCGATTTTTTTCCTCCCCCCCCCTTTTCCTGCC CCCCCTGTTTCCCCGTTTCGTACCCCTCCCCCTCCCCCTCTTTCCCCCCATGCCCCCCCA CCCCTCCTTGCTTTGTCCCCCCCCACCCTCCACACTCTTATATTTTACTCCATT |
Restriction Sites | NotI-NotI |
ACCN | NM_005004 |
Insert Size | 730 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_005004.2, NP_004995.1 |
RefSeq Size | 686 bp |
RefSeq ORF | 561 bp |
Locus ID | 4714 |
UniProt ID | O95169 |
Protein Families | Transmembrane |
Protein Pathways | Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease |
Gene Summary | Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). |
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Other Versions
SKU | Description | Size | Price |
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RC201166 | NDUFB8 (Myc-DDK-tagged)-Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa (NDUFB8), nuclear gene encoding mitochondrial protein |
CNY 3,600.00 |
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RC201166L3 | Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa (NDUFB8), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201166L4 | Lenti ORF clone of Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa (NDUFB8), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
RG201166 | NDUFB8 (tGFP-tagged) - Human NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 8, 19kDa (NDUFB8), nuclear gene encoding mitochondrial protein |
CNY 5,200.00 |