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ENPP1 (NM_006208) Human Untagged Clone
CAT＃: SC108777
ENPP1 (untagged)-Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1)
CNY 9,600.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARHR2; COLED; M6S1; NPP1; NPPS; PC-1; PCA1; PDNP1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_006208, the custom clone sequence may differ by one or more nucleotides
ATGGAGCGCGACGGCTGCGCGGGGGGCGGGAGCCGCGGCGGCGAGGGCGGGCGCGCTCCCCGGGAGGGCC CGGCGGGGAACGGCCGCGATCGGGGCCGCAGCCACGCTGCCGAGGCGCCCGGGGACCCGCAGGCGGCCGC GTCCTTGCTGGCCCCTATGGACGTGGGGGAGGAGCCGCTGGAGAAGGCGGCGCGCGCCCGCACTGCCAAG GACCCCAACACCTATAAAGTACTCTCGCTGGTATTGTCAGTATGTGTGTTAACAACAATACTTGGTTGTA TATTTGGGTTGAAACCAAGCTGTGCCAAAGAAGTTAAAAGTTGCAAAGGTCGCTGTTTCGAGAGAACATT TGGGAACTGTCGCTGTGATGCTGCCTGTGTTGAGCTTGGAAACTGCTGTTTAGATTACCAGGAGACGTGC ATAGAACCAGAACATATATGGACTTGCAACAAATTCAGGTGTGGTGAGAAAAGGTTGACCAGAAGCCTCT GTGCCTGTTCAGATGACTGCAAGGACAAGGGCGACTGCTGCATCAACTACAGTTCTGTGTGTCAAGGTGA GAAAAGTTGGGTAGAAGAACCATGTGAGAGCATTAATGAGCCACAGTGCCCAGCAGGGTTTGAAACGCCT CCTACCCTCTTATTTTCTTTGGATGGATTCAGGGCAGAATATTTACACACTTGGGGTGGACTTCTTCCTG TTATTAGCAAACTAAAAAAATGTGGAACATATACTAAAAACATGAGACCGGTATATCCAACAAAAACTTT CCCCAATCACTACAGCATTGTCACCGGATTGTATCCAGAATCTCATGGCATAATCGACAATAAAATGTAT GATCCCAAAATGAATGCTTCCTTTTCACTTAAAAGTAAAGAGAAATTTAATCCTGAGTGGTACAAAGGAG AACCAATTTGGGTCACAGCTAAGTATCAAGGCCTCAAGTCTGGCACATTTTTCTGGCCAGGATCAGATGT GGAAATTAACGGAATTTTCCCAGACATCTATAAAATGTATAATGGTTCAGTACCATTTGAAGAAAGGATT TTAGCTGTTCTTCAGTGGCTACAGCTTCCTAAAGATGAAAGACCACACTTTTACACTCTGTATTTAGAAG AACCAGATTCTTCAGGTCATTCATATGGACCAGTCAGCAGTGAAGTCATCAAAGCCTTGCAGAGGGTTGA TGGTATGGTTGGTATGCTGATGGATGGTCTGAAAGAGCTGAACTTGCACAGATGCCTGAACCTCATCCTT ATTTCAGATCATGGCATGGAACAAGGCAGTTGTAAGAAATACATATATCTGAATAAATATTTGGGGGATG TTAAAAATATTAAAGTTATCTATGGACCTGCAGCTCGATTGAGACCCTCTGATGTCCCAGATAAATACTA TTCATTTAACTATGAAGGCATTGCCCGAAATCTTTCTTGCCGGGAACCAAACCAGCACTTCAAACCTTAC CTGAAACATTTCTTACCTAAGCGTTTGCACTTTGCTAAGAGTGATAGAATTGAGCCCTTGACATTCTATT TGGACCCTCAGTGGCAACTTGCATTGAATCCCTCAGAAAGGAAATATTGTGGAAGTGGATTTCATGGCTC TGACAATGTATTTTCAAATATGCAAGCCCTCTTTGTTGGCTATGGACCTGGATTCAAGCATGGCATTGAG GCTGACACCTTTGAAAACATTGAAGTCTATAACTTAATGTGTGATTTACTGAATTTGACACCGGCTCCTA ATAACGGAACTCATGGAAGTCTTAACCACCTTCTAAAGAATCCTGTTTATACGCCAAAGCATCCCAAAGA AGTGCACCCCCTGGTACAGTGCCCCTTCACAAGAAACCCCAGAGATAACCTTGGCTGCTCATGTAACCCT TCGATTTTGCCGATTGAGGATTTTCAAACACAGTTCAATCTGACTGTGGCAGAAGAGAAGATTATTAAGC ATGAAACTTTACCCTATGGAAGACCTAGAGTTCTCCAGAAGGAAAACACCATCTGTCTTCTTTCCCAGCA CCAGTTTATGAGTGGATACAGCCAAGACATCTTAATGCCCCTTTGGACATCCTATACCGTGGACAGAAAT GACAGTTTCTCTACGGAAGACTTCTCCAACTGTCTGTACCAGGACTTTAGAATTCCTCTTAGTCCTGTCC ATAAATGTTCATTTTATAAAAATAACACCAAAGTGAGTTACGGGTTCCTCTCCCCACCACAACTAAATAA AAATTCAAGTGGAATATATTCTGAAGCTTTGCTTACTACAAATATAGTGCCAATGTACCAGAGTTTTCAA GTTATATGGCGCTACTTTCATGACACCCTACTGCGAAAGTATGCTGAAGAAAGAAATGGTGTCAATGTCG TCAGTGGTCCTGTGTTTGACTTTGATTATGATGGACGTTGTGATTCCTTAGAGAATCTGAGGCAAAAAAG AAGAGTCATCCGTAACCAAGAAATTTTGATTCCAACTCACTTCTTTATTGTGCTAACAAGCTGTAAAGAT ACATCTCAGACGCCTTTGCACTGTGAAAACCTAGACACCTTAGCTTTCATTTTGCCTCACAGGACTGATA ACAGCGAGAGCTGTGTGCATGGGAAGCATGACTCCTCATGGGTTGAAGAATTGTTAATGTTACACAGAGC ACGGATCACAGATGTTGAGCACATCACTGGACTCAGCTTCTATCAACAAAGAAAAGAGCCAGTTTCAGAC ATTTTAAAGTTGAAAACACATTTGCCAACCTTTAGCCAAGAAGACTGA >OriGene 5' read for NM_006208 unedited
GGCTGGGATTTGTAAACGACTTATATAGGCGGCCGCGCATTCGGCACGAGGGCCACGATG GTAGCGCGACGGCTGCGCGGGGGGCGGGAGCCGCGGCGGCGAGGGCGGGCGCGCTCCCCG GGAGGGCCCGGCGGGGAACGGCCGCGATCGGGGCCGCAGCCACGCTGCCGAGGCGCCCGG GGACCCGCAGGCGGCCGCGTCCTTGCTGGCCCTTATGGACGTGGGGGAGGAGCCGCTGGA GAAGGCGGCGCGCGCCCGCACTGCCAAGGACCCCAACACCTATAAAGTACTCTCGCTGGT ATTGTCAGTATGTGTGTTAACAACAATACTTGGTTGTATATTTGGGTTGAAACCAAGCTG TGCCAAAGAAGTTAAAAGTTGCAAAGGTCGCTGTTTCGAGAGAACATTTGGGAACTGTCG CTGTGATGCTGCCTGTGTTGAGCTTGGAAACTGCTGTTTAGATTACCAGGAGACGTGCAT AGAACCAGAACATATATGGACTTGCAACAAATTCAGGTGTGGTGAGAAAAGGTTGACCAG AAGCCTCTGTGCCTGTTCAGATGACTGCAAGGACCAGGGCGACTGCTGCATCAACTACAG TTCTGTGTGTCAAGGTGAGAAAAGTTGGGTAGAAGAACCATGTGAGAGCATTAATGAGCC ACAGTGCCCAGCAGGGTTTGAAACGCCTCCTACCCTCTTATTTTCTTTGGATGGATTCAG GGCAGAATATTTACACACTTGGGGTGGACTTCTTCCTGTTATTAGCAAACTAAANAAATG TGGAACATATACTANNAACATGAGACCGGTATATCCAACAAAAACTTTCCCCAATCACTA CAGCATTGTCACCGGGATGTATCCAGAATCTCATGGCATAATCGACAATAAATGTATGAT CCCCAAATGAATGC >OriGene 3' read for NM_006208 unedited
AGCTTTGNAACCGCGGCCGCAATCTANAAGTCGGTTTTTTTTTTTTTTTTTGATTACAAT GAAAAGAAATGAAACTCCAAGTTAAAAGATATCCAGAACCACTTTAAAATTCTCATAAAG ATAGTCACATAACATGGGCTACCCCAACCCTACTATAAGTCTACTGTTTGCCCAATGCCA TCAAGGTTTATTACCAATAGTAAGAAAGATTCAAATATCTGGTATCCAGACAATGTTCAC AGCTACTTCTCCTTTAAGAGAAGCAGAAAGGCAGTTTTAGGTGTGGTCTGTCTTTATTCC CCCGAACTTGATCAATGTATACAATGCTTACACATTCAAATATGTGCAAGATTCAACAGG AACACTCTCCACTGCTGTGCTGAGTACGCAAGTTTCCCTGAGATGTCCGCATCACCGAGG GCTCCGTTCTAACTCTGGTCGACAGTTTCTGAACAATGCAATAGTGTAGCTAGAGGACTA TATAAAATATAAGGTTCTCTCAAAAAGATTCATGGTGTTTGGGGATAAAAAACATATCAG TCTTCTTGGCTAAAGGTTGGCAAATGTGTTTTCAACTTTAAAATGTCTGAAACTGGCTCT TTTCTTTGTTGATAGAAGCTGAGTCCAGTGATGTGCTCAACATCTGTGATCCGTGCTCTG TGTAACATTAACAATTCTTCAACCCATGAGGAGTCATGCTTCCCATGCACACAGCTCTCG CTGTTATCAGTCCTGTGAGGCAAAATGAAAGCTAAGGTGTCTTAGGTTTTCACAGTGCAA AAGCGTCTGAGATGTATNCTTACAGCTTGTTAGCACATAAAGAAATGAGTTTGGATCAAA ATTCCTGGTTACGGATGACTCTNCTTTTTTGCTCAGATCTCTAAGGATCACACGTNCATC ATATCAAGTCAAACACAGGACACTGACGACATTGACACCATTTCTTTCTCANCTACTTTT TCCATAGGGTGTATGAAAGTACGCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_006208 |
| Insert Size | 3160 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006208.1, NP_006199.1 |
| RefSeq Size | 7442 bp |
| RefSeq ORF | 2622 bp |
| Locus ID | 5167 |
| UniProt ID | P22413 |
| Domains | SO, Endonuclease, Phosphodiest |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Metabolic pathways, Nicotinate and nicotinamide metabolism, Pantothenate and CoA biosynthesis, Purine metabolism, Riboflavin metabolism, Starch and sucrose metabolism |
| Gene Summary | This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Polyoxometalates-potent and selective ecto-nucleotidase inhibitors
,Lee, SY;Fiene, A;Li, W;Hanck, T;Brylev, KA;,
Biochemical Pharmacology
[ENPP1]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209222 | ENPP1 (Myc-DDK-tagged)-Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) |
CNY 9,592.00 |
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| RC209222L1 | Lenti ORF clone of Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), Myc-DDK-tagged |
CNY 11,992.00 |
|
| RC209222L2 | Lenti ORF clone of Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), mGFP tagged |
CNY 5,890.00 |
|
| RC209222L3 | Lenti ORF clone of Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), Myc-DDK-tagged |
CNY 11,992.00 |
|
| RC209222L4 | Lenti ORF clone of Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), mGFP tagged |
CNY 11,992.00 |
|
| RC229395 | ENPP1 (Myc-DDK-tagged)-Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) |
CNY 3,990.00 |
|
| RC229395L3 | Lenti ORF clone of Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RG209222 | ENPP1 (tGFP-tagged) - Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) |
CNY 11,192.00 |
|
| RG229395 | ENPP1 (tGFP-tagged) - Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) |
CNY 11,192.00 |
|
| SC328030 | ENPP1 (untagged)-Human ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1) |
CNY 7,220.00 |
