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BAF53A (ACTL6A) (NM_177989) Human Untagged Clone
CAT＃: SC108893
ACTL6A (untagged)-Human actin-like 6A (ACTL6A), transcript variant 2
CNY 2,950.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ACTL6; Arp4; ARPN-BETA; BAF53A; INO80K |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_177989 edited
GAATTCGGCACGAGGAGTCACTTCGCCAGTTAGCCCTTAGGGTAGGAGTCGCGCCGGCAG CAGCCATGAGCGGCGGCGTGTACGGGGGAGATGAAGTTGGAGCCCTTGTTTTTGACATTG GATCCTATACTGTGAGAGCTGGTTATGCTGGTGAGGACTGCCCCAAGGTGGATTTTCCTA CAGCTATTGGTATGGTGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCG ATAAAGGCAAACAAGGCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGA GGGAGAATATGGAGGCCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTT TCCAAGCTATTTTGGATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATC CTGTTCTCATGTCAGAGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGT TAATGTTTGAACACTACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAG CATTTGCTAATGGTCGTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTG CAATTCCAGTCCACGATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTG GAGACTTTATTACTATGCAGTGCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTC CTCCATATATGATTGCAACAAAAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAA GAAAAGAGAAGTTGCCTCAGGTTACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTA TCCAGGATTTTCAAGCTTCGGTACTTCAAGTGTCAGATTCAACTTATGATGAACAAGTGG CTGCACAGATGCCAACTGTTCATTATGAATTCCCCAATGGCTACAATTGTGATTTTGGTG CAGAGCGGCTAAAGATTCCAGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAG GAAACACAATGTTAGGAGTCAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTG ACATCAGACCAGGTCTCTATGGCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGA GTTTTACTGACAGGTTGAATAGAGAGCTGTCTCAGAAAACTCCTCCAAGTATGCGGTTGA AATTGATTGCAAATAATACAACAGTGGAACGGAGGTTTAGCTCATGGATTGGCGGCTCCA TTCTAGCCTCTTTGGGTACCTTTCAACAGATGTGGATTTCCAAGCAAGAATATGAAGAAG GAGGGAAGCAGTGTGTAGAAAGAAAATGCCCTTGAGAAAGAGTTCCCAAGCTTCTACCTT CCTTTTGTCACCTTACGTTTCATAGCTTTAGTATACTCAGGAAAAGAATGACCATCTTTT GTAGAATGTTTATACATTTTTGCATATTTCAATTTCCACTTAAATTTTTTAAAGCTTTAA CTGGCTCTATAAATTAAGTTTGTGCTTTCCTTGAAATGCACTTATTCTTATTACAAGCAT TTTATAATTTTGTATAAATGTCTATTTTCTCTAAATATTTTGCTTTCAGTAAAATGCTTT CCAACTCTGTTTAGTGTATTAATTACCAGTGGATTGGTAGAACTGCTTTTTATTGACTAG TAAAAGTTACTGCCTATGCTTTTTACCTTAGGCTTACAGAATTAAATAAAAATTAGCCAT TCCAGAAATAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_177989 unedited
AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGTGGCTGAGCTCCGGGGT GTGTGGACGCCGCTTTGTTGCCTGAGATGAAGTTGGAGCCCTTGTTTTTGACATTGGATC CTATACTGTGAGAGCTGGTTATGCTGGTGAGGACTGCCCCAAGGTGGATTTTCCTACAGC TATTGGTATGGTGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAA AGGCAAACAAGGCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGA GAATATGGAGGCCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCA AGCTATTTTGGATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGT TCTCATGTCAGAGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAAT GTTTGAACACTACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATT TGCTAATGGTCGTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAAT TCCAGTCCACGATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGA CTNTATTACTATGCAGTGCAGAGAACTCTTCCAAGAATGAATATTGAATTGGTTCCTCCA TATATGATTGCAACNAAAGAAGCTGTTCGTGAAGGATCTNCAGCANACTGGAAAAGAAAA GAGAAGTTGCCTCANGTTACGAGGGTCTGGCACATTATATGTGTAAATTGTGTATNCCAG ATTTTCAAAGCTTCGTACTTCAGTGTCAGATTCACTTATGATGAACAGTGGCTGCACANA TGCCACTGTTCATATTGAATCCCCATGGCTACATTGTGATTTGGTGCANAGCGCTAAGAT TCA >OriGene 3' read for NM_177989 unedited
TAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTATATTTCTGGAATGGCTAATTTTTATTTAATTCTGTAAGCCTAAGGGAAAAAGCA TAGGCAGTAACTTTTACTAGTCAATAAAAAGCAGTTCTACCAATCCACTGGTAATTAATA CACTAAACAGAGTTGGAAAGCATTTTACTGAAAGCAAAATATTTAGAGAAAATAGACATT TATACAAAATTATAAAATGCTTGTAATAAAAATAAGGGCATTTCAAGGAAAGCACAAACT TAATTTATAGAGCCAGTTAAAGCTTTAAAAAATTTAAGGGGAAATTGAAATATGCAAAAA TGTATAAACATTCTACAAAAGATGGGCATTCTTTTCCTGAGTATACTAAAGCTATGAAAC GTAAGGGGACAAAAGGAAGGTAAAAGCTTGGGAACTCTTTCTCAAGGGCATTTTCTTTCT ACACACTGCTTCCCTCCTTCTTCATATTCTTGCTTGGAAATCCACATCTGTTGAAAGGTA CCCAAAGAGGCTAAAATGGAGCCGCCAATCCATGAGCTAAACCTCCGTTCCACTGTTGTA TTATTTGCAATCAATTTCAACCGCATACTTGGAGGAGTTTTCTGAGACAGCTCTCTATTC AACCTGTCAGTAAAACTCTGTATTAGTGTGTTTCCTCCTGCCACTATTACACTGCCATAG AGACCTGGTCTGATGTCAATATCACACATCCCAACACTTGTGGTGACAACATGACTGACT CCTAACAATGGGTTTCCTGATAACCCCTTTACATTGGAAGGGTCAAAATATCCTTTCTGG ATCTTTAGCGCTCTGCACCAAATCACATTGTAGCCATTGGGGAATCATAATGAACAGTTG CATCTGTGCAGCCACTGTCATCATAGTTGGATCTGACCTTGAGTACC |
| Restriction Sites | NotI-NotI |
| ACCN | NM_177989 |
| Insert Size | 1950 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_177989.1, NP_817126.1 |
| RefSeq Size | 1737 bp |
| RefSeq ORF | 1164 bp |
| Locus ID | 86 |
| UniProt ID | O96019 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region that results in the use of a downstream start codon, compared to variant 1. Variants 2 and 3 both encode isoform b, which has a shorter N-terminus when compared to isoform a. This variant is also known as Nbeta2 and isoform b is also known as hArpNbetaS. Isoform b is found mostly in the cytoplasm. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201689 | ACTL6A (Myc-DDK-tagged)-Human actin-like 6A (ACTL6A), transcript variant 2 |
CNY 3,656.00 |
|
| RC201689L3 | Lenti ORF clone of Human actin-like 6A (ACTL6A), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC201689L4 | Lenti ORF clone of Human actin-like 6A (ACTL6A), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG201689 | ACTL6A (tGFP-tagged) - Human actin-like 6A (ACTL6A), transcript variant 2 |
CNY 4,370.00 |
