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Aspartate beta hydroxylase (ASPH) (NM_032466) Human Untagged Clone
CAT＃: SC108955
ASPH (untagged)-Human aspartate beta-hydroxylase (ASPH), transcript variant 3
CNY 2,400.00
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | AAH; BAH; CASQ2BP1; FDLAB; HAAH; JCTN; junctin |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC108955 sequence for NM_032466 edited (data generated by NextGen Sequencing)
ATGGCCCAGCGTAAGAATGCCAAGAGCAGCGGCAACAGCAGCAGCAGCGGCTCCGGCAGC GGTAGCACGAGTGCGGGCAGCAGCAGCCCCGGGGCCCGGAGAGAGACAAAGCATGGAGGA CACAAGAATGGGAGGAAAGGCGGACTCTCAGGAACTTCATTCTTCACGTGGTTTATGGTG ATTGCATTGCTGGGCGTCTGGACATCTGTAGCTGTCGTTTGGTTTGATCTTGTTGACTAT GAGGAAGTTCTAGGAAAACTAGGAATCTATGATGCTGATGGTGATGGAGATTTTGATGTG GATGATGCCAAAGTTTTATTAGGACTTAAAGAGAGATCTACTTCAGAGCCAGCAGTCCCG CCAGAAGAGGCTGAGCCACACACTGAGCCCGAGGAGCAGGTTCCTGTGGAGGCAGAACCC CAGAATATCGAAGATGAAGCAAAAGAACAAATTCAGTCCCTTCTCCATGAAATGGTACAC GCAGAACATGTTGAGGGAGAAGACTTGCAACAAGAAGATGGACCCACAGGAGAACCACAA CAAGAGGATGATGAGTTTCTTATGGCGACTGATGTAGATGATAGATTTGAGACCCTGGAA CCTGAAGTATCTCATGAAGAAACCGAGCATAGTTACCACGTGGAAGAGACAGTTTCACAA GACTGTAATCAGGATATGGAAGAGATGATGTCTGAGCAGGAAAATCCAGATTCCAGTGAA CCAGTAGTAGAAGATGAAAGATTGCACCATGATACAGATGATGTAACATACCAAGTCTAT GAGGAACAAGCAGTATATGAACCTCTAGAAAATGAAGGGATAGAAATCACAGAAGTAACT GCTCCCCCTGAGGATAATCCTGTAGAAGATTCACAGGTAATTGTAGAAGAAGTAAGCATT TTTCCTGTGGAAGAACAGCAGGAAGTACCACCAGATACTTAA Clone variation with respect to NM_032466.3 >OriGene 5' read for NM_032466 unedited
ATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGGTGCTGCAGGCGGT GCTGAGGCACAGGGTCTGCTGCAGGAGCAGCGGCCCGAACCCGCTCCAGCGGCCCGCCGC CGCCAGCGGCTCCCGCGTCGCGTGTGTACCCCCGCGCACTGAAGGAGGTCCACCAGCCCT CACCAGCCCCCGCGGACCGTGCAATGGCCCAGCGTAAGAATGCCAAGAGCAGCGGCAACA GCAGCAGCAGCGGCTCCGGCAGCGGTAGCACGAGTGCGGGCAGCAGCAGCCCCGGGGCCC GGAGAGAGACAAAGCATGGAGGACACAAGAATGGGAGGAAAGGCGGACTCTCAGGAACTT CATTCTTCACGTGGTTTATGGTGATTGCATTGCTGGGCGTCTGGACATCTGTAGCTGTCG TTTGGTTTGATCTTGTTGACTATGAGGAAGTTCTAGGAAAACTAGGAATCTATGATGCTG ATGGTGATGGAGATTTTGATGTGGATGATGCCAAAGTTTTATTAGGACTTAAAGAGAGAT CTACTTCAGAGCCAGCAGTCCCGCCAGAAGAGGCTGAGCCACACACTGAGCCCGAGGAGC AGGTTCCTGTGGAGGCAGAACCCCAGAATATCGAAGATGAAGCAAAAGAACAAATTCAGT CCCCTTCTCCATGAAATGGTACACGCCAGACATGTTGAGGGGAGAAGACTTGCAACAAGA AGATGGGCCCCCAGGNAGACCCCCAACCAGAGGATGATGAGGTCTCTATTGCGACTGATG GAGATGATAAATTTGAGACCCTTGGACCTGGAGTTTCTTATGAAAAAAACCGAGCCTAGT TCCACGTGGGAAGAGACAGGTTCCCAAAACTGGGATCACGAATTGG >OriGene 3' read for NM_032466 unedited
NCCCGTAATCCTTGNCCGCGGCCGCATTTNANGATCGCGNTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTTTTTTTTTAAACCTTCGGGATTTAATGGCCCCCAACATTT AATAAAAAACATTTACATTATTTTTAAAAAATCCACCGATGTTGCACAATGGCACATATT TGTAAAGCACAAGGCTCATTAATTCAAATATTTGGAATTCAAAAATATTTAAAGTTAACC ACAGCATAATGAATCCTCAACGTCCAGAGTTCTACAAAAATCCAGCAAAACTTACTTTTA CCCATTCATCAGTTCTATGGCACTCCCTTAGTTTCCCTAAAAAAAAAAAGGGTTTAAAAA AAGGAGCTTTTTTATATTCCCAAAAGGGAGAAGTTTTATTAAAATTTGGGGAAAAATTTT TTGATCACCCGACCGGCCCAGTTTTAAAAAAAAAAGGGGCTTCTTAAAAAACCAAAATTT TTCAGGGTTTAATTTACCACTGGGGGACCCCCCGGCGGGGGTTCCCATCACTTGGGTTAA AAACCTCTGGGCCTCTTGGGGGGAAAAAAAAATAAAAAAGATTTTTTTTTGTTTTTTACC CCTCCAAAAACCGGGGGGGGAGAAAAGCGCCCCCCTTCTTTTTATTGGGTGTACAACAAA AAAAAAAAAGACTCCCCCCTCCTCTATTAATTTGCTCACCACACAACCCCCTCTTTTTTT AGGAGGGTGTTCGTTCTTATAAAAAGAGGGCCAACAAGAGGGCGCCTCCCTTCACAAAGA AAGAGGAGGGGGGGGCGCCCCACTCCTCTTCTTCTTTTGTTTCGCAACAGAGAGTGTTTA TTTTTCTTCTCCCANACGAAACAATATATATTTTTTTGTGGTGGCGCCCCCACATATATA CCTCCCTCTCTTTCTATAATAAAGAACAAACAAATATCTTTTTCGTTGTTGTAAACACAA AAAAAANAGAGCGTCTCTCTCTGCGCGTGCGCGAGATAATATACAACAACACACACAAAG ACGCCGTCCTCGTGGGTGGCTCTTNCTTTCTANACACACCCCCTCCACACACACACCACC NGCTCTGTCGAGGGGGGGTGGTTGTGGTGGTTTCTTCTTATAATAGAGAGAGCACGATCG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_032466 |
| Insert Size | 2930 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032466.1, NP_115855.1 |
| RefSeq Size | 2680 bp |
| RefSeq ORF | 942 bp |
| Locus ID | 444 |
| UniProt ID | Q12797 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | This gene is thought to play an important role in calcium homeostasis. The gene is expressed from two promoters and undergoes extensive alternative splicing. The encoded set of proteins share varying amounts of overlap near their N-termini but have substantial variations in their C-terminal domains resulting in distinct functional properties. The longest isoforms (a and f) include a C-terminal Aspartyl/Asparaginyl beta-hydroxylase domain that hydroxylates aspartic acid or asparagine residues in the epidermal growth factor (EGF)-like domains of some proteins, including protein C, coagulation factors VII, IX, and X, and the complement factors C1R and C1S. Other isoforms differ primarily in the C-terminal sequence and lack the hydroxylase domain, and some have been localized to the endoplasmic and sarcoplasmic reticulum. Some of these isoforms are found in complexes with calsequestrin, triadin, and the ryanodine receptor, and have been shown to regulate calcium release from the sarcoplasmic reticulum. Some isoforms have been implicated in metastasis. [provided by RefSeq, Sep 2009] Transcript Variant: This variant (3) uses a distinct 3' splice pattern that lacks many coding exons, compared to variant 1. The encoded isoform (c), also known as humbug, is identical to the N-terminal and central region of isoform a. Because this transcript variant lacks the alternative 3' terminal exon which encodes a catalytic domain for isoform a, isoform c is considered a noncatalytic isoform. This variant is widely expressed among heart, placenta, skeletal muscle, kidney, and lung tissues. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211817 | ASPH (Myc-DDK-tagged)-Human aspartate beta-hydroxylase (ASPH), transcript variant 3 |
CNY 2,400.00 |
|
| RC211817L1 | Lenti ORF clone of Human aspartate beta-hydroxylase (ASPH), transcript variant 3, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC211817L2 | Lenti ORF clone of Human aspartate beta-hydroxylase (ASPH), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RC211817L3 | Lenti ORF clone of Human aspartate beta-hydroxylase (ASPH), transcript variant 3, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211817L4 | Lenti ORF clone of Human aspartate beta-hydroxylase (ASPH), transcript variant 3, mGFP tagged |
CNY 5,890.00 |
|
| RG211817 | ASPH (tGFP-tagged) - Human aspartate beta-hydroxylase (ASPH), transcript variant 3 |
CNY 4,370.00 |
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