CDC25C (NM_001790) Human Untagged Clone

CAT#: SC109054

CDC25C (untagged)-Human cell division cycle 25 homolog C (S. pombe) (CDC25C), transcript variant 1



  "NM_001790" in other vectors (6)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit monoclonal antibody against Cdc25C(E302)
    • 100 ul

CNY 6,468.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms CDC25; PPP1R60
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC109054 sequence for NM_001790 edited (data generated by NextGen Sequencing)
ATGTCTACGGAACTCTTCTCATCCACAAGAGAGGAAGGAAGCTCTGGCTCAGGACCCAGT
TTTAGGTCTAATCAAAGGAAAATGTTAAACCTGCTCCTGGAGAGAGACACTTCCTTTACC
GTCTGTCCAGATGTCCCTAGAACTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTA
AGCATTTTGTCTGGAGGAACCCCAAAACGTTGCCTCGATCTTTCGAATCTTAGCAGTGGG
GAGATAACTGCCACTCAGCTTACCACTTCTGCAGACCTTGATGAAACTGGTCACCTGGAT
TCTTCAGGACTTCAGGAAGTGCATTTAGCTGGGATGAATCATGACCAGCACCTAATGAAA
TGTAGCCCAGCACAGCTTCTTTGTAGCACTCCGAATGGTTTGGACCGTGGCCATAGAAAG
AGAGATGCAATGTGTAGTTCATCTGCAAATAAAGAAAATGACAATGGAAACTTGGTGGAC
AGTGAAATGAAATATTTGGGCAGTCCCATTACTACTGTTCCAAAATTGGATAAAAATCCA
AACCTAGGAGAAGACCAGGCAGAAGAGATTTCAGATGAATTAATGGAGTTTTCCCTGAAA
GATCAAGAAGCAAAGGTGAGCAGAAGTGGCCTATATCGCTCCCCGTCGATGCCAGAGAAC
TTGAACAGGCCAAGACTGAAGCAGGTGGAAAAATTCAAGGACAACACAATACCAGATAAA
GTTAAAAAAAAGTATTTTTCTGGCCAAGGAAAGCTCAGGAAGGGCTTATGTTTAAAGAAG
ACAGTCTCTCTGTGTGACATTACTATCACTCAGATGCTGGAGGAAGATTCTAACCAGGGG
CACCTGATTGGTGATTTTTCCAAGGTATGTGCGCTGCCAACCGTGTCAGGGAAACACCAA
GATCTGAAGTATGTCAACCCAGAAACAGTGGCTGCCTTACTGTCGGGGAAGTTCCAGGGT
CTGATTGAGAAGTTTTATGTCATTGATTGTCGCTATCCATATGAGTATCTGGGAGGACAC
ATCCAGGGAGCCTTAAACTTATATAGTCAGGAAGAACTGTTTAACTTCTTTCTGAAGAAG
CCCATCGTCCCTTTGGACACCCAGAAGAGAATAATCATCGTGTTCCACTGTGAATTCTCC
TCAGAGAGGGGCCCCCGAATGTGCCGCTGTCTGCGTGAAGAGGACAGGTCTCTGAACCAG
TATCCTGCATTGTACTACCCAGAGCTATATATCCTTAAAGGCGGCTACAGAGACTTCTTT
CCAGAATATATGGAACTGTGTGAACCACAGAGCTACTGCCCTATGCATCATCAGGACCAC
AAGACTGAGTTGCTGAGGTGTCGAAGCCAGAGCAAAGTGCAGGAAGGGGAGCGGCAGCTG
CGGGAGCAGATTGCCCTTCTGGTGAAGGACATGAGCCCATGA

Clone variation with respect to NM_001790.3
>OriGene 5' read for NM_001790 unedited
CCGTCAGAAGTGTATACGACTCATATAGGCGGCCGCGNAATCGGCACGAGGCTTTGGCCT
TCTGCTCAGGGAAGACTCTGAGTCCGACGTTGGCCTACCCAGTCGGAAGGCAGAGCTGCA
ATCTAGTTAACTACCTCCTTTCCCCTAGATTTCCTTTCATTCTGCTCAAGTCTTCGCCTG
TGTCCGATCCCTATCTACTTTCTCTCCTCTTGTAGGCAAGCCTCAGACTCCAGGCTTGAG
CTAGGTTTTGTTTTTCTCCTGGTGAGAATTCGAAGACCATGTCTACGGAACTCTTCTCAT
CCACAAGAGAGGAAGGAAGCTCTGGCTCAGGACCCAGTTTTAGGTCTAATCAAAGGAAAA
TGTTAAACCTGCTCCTGGAGAGAGACACTTCCTTTACCGTCTGTCCAGATGTCCCTAGAA
CTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTAAGCATTTTGTCTGGAGGAACCC
CAAAACGTTGCCTCGATCTTTCGAATCTTAGCAGTGGGGAGATAACTGCCACTCAGCTTA
CCACTTCTGCAGACCTTGATGAAACTGGTCACCTGGATTCTTCAGGACTTCAGGAAGTGC
ATTTAGCTGGGATGAATCATGACCAGCACCTAATGAAATGTAGCCCAGCACAGCTTCTTT
GTAGCACTCCGAATGGTTTGGACCGTGGCCATAGAAAGAGAGATGCAATGTGTAGTTCAT
CTGCAAATAAAGAAAATGACAATGGAAACTTGNTGGACAGTGAAATGAAATATTTGGGCA
GTCCCATTACTACTGNTCCCAAATTGGATAGAAATCCAAACCTAGGAGAAGACCAGGCAG
AAGAGATTTCAGATGAATTATGGGAGTTTTCCCTTGAAGATCAAGAGCCACAGTGAGCCT
>OriGene 3' read for NM_001790 unedited
TATGGCCGCGGCCGCAATCTAGAGTCGAGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCAAAAACATCTTTTAATAATCTTGGGGTTG
GCCCTCCAAAAGGCCCCTTTTTGCTCAAGGGTTTTGCAGGGGCTTTTGGGGACTTGTTAA
CAACCCAGGGCTGGAAAGGTATCATGGGCTCATGTCCTTCACCAAAAGGGGAATTTGGTC
CCCCAAGTGGCGGTCCCCTTTCTGGACTTTGGTTTGGGTTCGACACCCCAACAACTCAAT
CTTGGGGGCCCGAAGAAGCATAAGGCAAAAACTCTGGGGGTCACACAGTTCCCTATATTC
TGGAAAAAAATCTCTGTAACCCCCTTTAAAGAAATATAACTCTGGGGAAAACAAAGCAGG
ATACTGGGTCAAAAACCTGTCCTTTTTACCCAAACAGGGGGACATTTGGGGGCCCCTCTC
TGAGGAGAATTCACAGGGGAACACCATGATTATTCTCTTCTGGGGGGCCAAAGGGACCAA
GGGCTTCTTCAAAAAGAAATTAAACAGGTCTTCCTGACTATATAAAGTTAAAGCTCCCTG
GAAGGGGCCCCCCCGAACCCATATGGGTAGCGACCATCAATGACCTAAAACTTTTCAATC
AAACCCTGGAACTTCCCCCACAGTAAAGCAACCCCCTGGTTCTGGGTTGACATACTTCAG
AACTTGGTGTTTCCCTGACACGGTTGGCAGCGCACATACCTTTGAAAAAACACCCATCAA
GTGCCCCTGGTTAAAAACCTCCTCCCACCTCTGAGTGAAAGTATGCCCCCACAGAGACTG
CCTTCTTTAACACTAACCCTTCCTGACCTTCCCTTGGCCAAAAAAAACTTTTTTTTACTT
A
Restriction Sites NotI-NotI     
ACCN NM_001790
Insert Size 1860 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001790.2, NP_001781.1
RefSeq Size 2115 bp
RefSeq ORF 1422 bp
Locus ID 995
UniProt ID P30307
Domains RHOD
Protein Families Druggable Genome, Phosphatase, Stem cell - Pluripotency
Protein Pathways Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
Gene Summary This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]
Transcript Variant: This variant (1) encodes isoform a. Variants 1 and 3 encode the same protein (isoform a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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