GERP (TRIM8) (NM_030912) Human Untagged Clone
CAT#: SC109091
TRIM8 (untagged)-Human tripartite motif containing 8 (TRIM8)
CNY 6,168.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | GERP; RNF27 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC109091 sequence for NM_030912 edited (data generated by NextGen Sequencing)
ATGGCGGAGAATTGGAAGAACTGCTTCGAGGAGGAGCTCATCTGCCCTATCTGCCTGCAC GTTTTCGTGGAGCCAGTGCAGCTGCCGTGCAAACACAACTTCTGCCGGGGCTGCATCGGC GAGGCGTGGGCCAAGGACAGCGGCCTCGTACGCTGCCCAGAGTGCAACCAGGCCTACAAC CAGAAGCCGGGCCTGGAGAAGAACCTGAAGCTCACCAACATCGTGGAGAAGTTCAATGCC CTGCACGTGGAGAAGCCGCCGGCGGCGCTGCACTGCGTGTTCTGCCGCCGCGGCCCCCCG CTGCCCGCGCAGAAGGTCTGCCTGCGCTGCGAGGCGCCCTGCTGCCAGTCCCACGTGCAG ACGCACCTGCAGCAGCCCTCCACCGCCCGCGGGCACCTCCTGGTGGAGGCGGACGACGTG CGGGCCTGGAGCTGCCCGCAGCACAACGCCTACCGCCTCTACCACTGCGAGGCCGAGCAG GTGGCCGTGTGCCAGTACTGCTGCTACTACAGCGGCGCGCATCAGGGACACTCGGTGTGC GACGTGGAGATCCGAAGGAATGAAATCCGGAAGATGCTCATGAAGCAGCAGGACCGGCTG GAGGAGCGAGAGCAGGACATTGAGGACCAGCTGTACAAACTCGAGTCAGACAAGCGCCTG GTGGAGGAGAAAGTGAACCAACTGAAGGAGGAAGTTCGGCTGCAGTACGAGAAGCTGCAC CAGCTGCTGGACGAGGACCTGCGGCAGACAGTGGAGGTCCTAGACAAGGCCCAGGCCAAG TTCTGCAGCGAGAACGCAGCGCAGGCGCTGCACCTCGGGGAGCGCATGCAGGAGGCCAAG AAGCTGCTGGGCTCCCTGCAGCTGCTCTTTGATAAGACGGAGGATGTCAGCTTCATGAAG AACACCAAGTCTGTGAAAATCCTGATGGACAGGACCCAGACCTGCACGAGCAGCAGCCTT TCCCCCACTAAGATCGGCCACCTGAACTCCAAGCTCTTCCTGAACGAAGTGGCCAAGAAG GAGAAGCAGCTGCGGAAAATGCTAGAAGGCCCCTTCAGCACGCCGGTGCCCTTCCTGCAG AGTGTCCCCCTGTACCCTTGCGGCGTGAGCAGCTCTGGGGCGGAAAAGCGCAAGCACTCA ACGGCCTTCCCAGAGGCCAGTTTCCTAGAGACGTCGTCGGGCCCTGTGGGCGGCCAGTAC GGGGCGGCGGGCACAGCCAGCGGTGAGGGCCAGTCTGGGCAGCCCCTGGGGCCCTGCAGC TCCACGCAGCACTTGGTGGCCCTGCCGGGCGGCGCCCAACCAGTGCACTCAAGCCCCGTG TTCCCCCCATCGCAGTATCCCAATGGCTCCGCCGCCCAGCAGCCCATGCTCCCCCAGTAT GGCGGCCGCAAGATTCTCGTCTGTTCTGTGGACAACTGTTACTGTTCTTCCGTGGCCAAC CATGGCGGCCACCAGCCCTACCCCCGCTCCGGCCACTTTCCCTGGACAGTGCCCTCGCAG GAGTACTCACACCCGCTCCCGCCCACACCCTCCGTCCCCCAGTCCCTTCCCAGCCTGGCG GTCAGAGACTGGCTTGACGCCTCCCAGCAGCCCGGCCACCAGGATTTCTACAGGGTGTAT GGGCAGCCGTCCACCAAACACTACGTGACGAGCTAA Clone variation with respect to NM_030912.2 >OriGene 5' read for NM_030912 unedited
TATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCTCCGGACGGACCCCC GGGGCTGGGGAGTCGGGGAGGCCTGCCCCGGCCCCCTGCCCGCGGCCGCCATGGCGGAGA ATTGGAAGAACTGCTTCGAGGAGGAGCTCATCTGCCCTATCTGCCTGCACGTTTTCGTGG AGCCAGTGCAGCTGCCGTGCAAACACAACTTCTGCCGGGGCTGCATCGGCGAGGCGTGGG CCAAGGACAGCGGCCTCGTACGCTGCCCAGAGTGCAACCAGGCCTACAACCAGAAGCCGG GCCTGGAGAAGAACCTGAAGCTCACCAACATCGTGGAGAAGTTCAATGCCCTGCACGTGG AGAAGCCGCCGGCGGCGCTGCACTGCGTGTTCTGCCGCCGCGGCCCCCCGCTGCCCGCGC AGAAGGTCTGCCTGCGCTGCGAGGCGCCCTGCTGCCAGTCCCACGTGCAGACGCACCTGC AGCAGCCCTCCACCGCCCGCGGGCACCTCCTGGTGGAGGCGGACGACGTGCGGGCCTGGA GCTGCCCGCAGCACAACGCCTACCGCCTCTACCACTGCGAGGCCGAGCAGGTGGCCGTGT GCCAGTACTGCTGCTACTACAGCGGCGCGCATCANGGACACTCGGTGTGCGACGTGGAGA TCCGAAGGAATGAAATCCGGAAGATGCTCATGAAGCANCANGACCGGCTGGAGGAGCGAG AGCAGGACATTGAGGACCAGCTGTACAAACTCGAGTCAGCAAGCGCCTGGTGGANGGAGA AGTGACCACTGGGGAAGGAAGTCGGCTGCAGTACGAGAACTGCACCGCTGCTGGACGAGG ACTGCGCAACANGGGNAGGTCTAAACAGGCCCAGCCCAGTCTGCACGAACNCCACGCAGC GCTCCCTTGGGGAGCCATGCAGAGGCAAAACTGCTGGCTCTGCACTGTCTGATAAACGAG GAGATA >OriGene 3' read for NM_030912 unedited
NGGGTGGTTNNGGGNNNNNNNNTTATTTAATTNNNATGGTTTGTCATCTTTATTCCCCTT GTTGTTATNATAATTTTATTATTTGCTTTTGCTGTGGTCACTATCATTGGCGAAGTTCCT TTTCCCCTTTCCTGGGACTAGGGGTCCTGTCTTTAAAAGATCCACTCTGAGCTGAAGTGC CAAAACTGCTTGAGAATGGCCTTTGAACTCCGTCTGGGAAGATGGTGAGGAGAAGGAAGC AGGAGAAATCATTCTTGGTTTAAAAAAATTTAAAAAAACAAAAACAAAAGTCGCACAATC TAAAGGTCATTCCTTACTCGATCAGTGATCAAACTGCCCAAAAGGAAAACAAGAGGAGAA ACAAGCCCGGGGCCCTGGGCGTTCCTCCCGGGTGGGGTAAGAAGGGTGGGACCCCCAAGC TTTCAGGGAAGCCGAGGTTCCTTCCAGCCACGGGGGTGTGAAGGGGAAGGAGTAGAGGAA AAAAGGAGAAACAGAGAGAGAGCTCAGCGCCTTGACACAGACACTTTCCCATCTCCAGGG CCACCTGCCGTCCTGGCCCAAGCCAGCTCCCACTGTCACCTCTGCGTCCAGGAGATTCAT AATAAAAAAAAATCAAAAACAAAGCCCAAAACCAAAACAAAATCCAAAAAGGAAAATCAC CTGGGGCCAATGGCAATGAACGACACAAGGGCACGCCGACGGAACGGTATAACGCGCACG TTCTCTTGGCTGGCATCACCCCCCCCAACCCCGGGGGGCCTGGGGGAGGCAAAAATTCCC CCACCCGACCCCCCCTCCCCTGCCTTGGGCGCTTACCCCTCCCTCCCCCTACCTCCTCTC GCTCGGACCCGCTTTTCCCCATTCACTCCCCCCGCACTCAAATCCTTCGGTGGCGCCCGG TCCTGTCTTCCGGAGAGCCTTCACAAACCTTATCCTTCTTCCACCCACCCACTCCTCGCC CCAAGAGCACTACGTCGGAACTGAGATAGATG |
Restriction Sites | NotI-NotI |
ACCN | NM_030912 |
Insert Size | 2330 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_030912.1, NP_112174.1 |
RefSeq Size | 2645 bp |
RefSeq ORF | 1656 bp |
Locus ID | 81603 |
UniProt ID | Q9BZR9 |
Domains | RING |
Gene Summary | This gene encodes a member of the tripartite motif (TRIM) protein family. Based on similarities to other proteins, the encoded protein is suspected to be an E3 ubiquitin-protein ligase. Regulation of this gene may be altered in some cancers. Mutations resulting in a truncated protein product have been observed in early-onset epileptic encephalopathy (EOEE). [provided by RefSeq, Sep 2016] Transcript Variant: This variant (1) encodes the longer isoform (1). |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC205812 | TRIM8 (Myc-DDK-tagged)-Human tripartite motif containing 8 (TRIM8) |
CNY 6,152.00 |
|
RC205812L1 | Lenti ORF clone of Human tripartite motif containing 8 (TRIM8), Myc-DDK-tagged |
CNY 8,552.00 |
|
RC205812L2 | Lenti ORF clone of Human tripartite motif containing 8 (TRIM8), mGFP tagged |
CNY 8,552.00 |
|
RC205812L3 | Lenti ORF clone of Human tripartite motif containing 8 (TRIM8), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC205812L4 | Lenti ORF clone of Human tripartite motif containing 8 (TRIM8), mGFP tagged |
CNY 5,890.00 |
|
RG205812 | TRIM8 (tGFP-tagged) - Human tripartite motif containing 8 (TRIM8) |
CNY 7,752.00 |