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KRAS (NM_004985) Human Untagged Clone
CAT＃: SC109374
KRAS (untagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b
CNY 3,600.00
| Cited in 4 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C-K-RAS; c-Ki-ras2; CFC2; K-Ras; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RALD; RASK2 |
| Vector | pCMV6-XL6 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_004985 edited
GAATTCGGCACGAGGCGGCGGAGGCAGCAGCGGCGGCGGCAGTGGCGGCGGCGAAGGTGG CGGCGGCTCGGCCAGTACTCCCGGCCCCCGCCATTTCGGACTGGGAGCGAGCGCGGCGCA GGCACTGAAGGCGGCGGCGGGGCCAGAGGCTCAGCGGCTCCCAGGTGCGGGAGAGAGGCC TGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGTG CCTTGACGATACAGCTAATTCAGAATCATTTTGTGGACGAATATGATCCAACAATAGAGG ATTCCTACAGGAAGCAAGTAGTAATTGATGGAGAAACCTGTCTCTTGGATATTCTCGACA CAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTACATGAGGACTGGGGAGGGCT TTCTTTGTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTCACCATTATAGAG AACAAATTAAAAGAGTTAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAAAT GTGATTTGCCTTCTAGAACAGTAGACACAAAACAGGCTCAGGACTTAGCAAGAAGTTATG GAATTCCTTTTATTGAAACATCAGCAAAGACAAGACAGGGTGTTGATGATGCCTTCTATA CATTAGTTCGAGAAATTCGAAAACATAAAGAAAAGATGAGCAAAGATGGTAAAAAGAAGA AAAAGAAGTCAAAGACAAAGTGTGTAATTATGTAAATACAATTTGTACTTTTTTCTTAAG GCATACTAGTACAAGTGGTAATTTTTGTACATTACACTAAATTATTAGCATTTGTTTTAG CATTACCTAATTTTTTTCCTGCTCCATGCAGACTGTTAGCTTTTACCTTAAATGCTTATT TTAAAATGACAGTGGAAGTTTTTTTTTCCTCGAAGTGCCAGTATTCCCAGAGTTTTGGTT TTTGAACTAGCAATGCCTGTGAAAAAGAAACTGAATACCTAAGATTTCTGTCTTGGGGTT TTTGGTGCATGCAGTTGATTACTTCTTATTTTTCTTACCAATTGTGAATGTTGGTGTGAA ACAAATTAATGAAGCTTTTGAATCATCCCTATTCTGTGTTTTATCTAGTCACATAAATGG ATTAATTACTAATTTCAGTTGAGACCTTCTAATTGGTTTTTACTGAAACATTGAGGGAAC ACAAATTTATGGGCTTCCTGATGATGATTCTTCTAGGCATCATGTCCTATAGTTTGTCAT CCCTGACGAATGTAAAGXXXXXXXXXXXXXXXXXXXXXXXXAATGGTTTCTTGATAATTG TGTAGTAATGTTTTTTAGAACCCAGCAGTTACCTTAAAGCTGAATTTATATTTAGTAACT TCTGTGTTAATACTGGATAGCATGAATTCTGCATTGAGAACCTGAATAGCTGTCATAAAA TGAAACTTTCTTTCTAAAGAAAGATACTCACATGAGTTCTTGAAGAATAGTCATAACTAG ATTAAGATCTGTGTTTTAGTTTAATAGTTTGAAGTGCCTGTTTGGGATAATGATAGGTAA TTTAGATGAATTTAGGGGAAAAAAGTTATCTGCAGAAATGTTGAGGGCCCATCTCTCCCC CCACACCCCCACAGAGCTAACTGGGTTACAGTGTTTTATCCGAAAGTTTCCAATTCCACT GTCTTGTGTTTTCATGTTGAAAATACTTTTGCATTTTTCCTTTGAGTGCCAATTTCTTAC TAGTACTATTTCTTAATGTAACATGTTTACCTGGAATGTATTTTAACTATTTTTGTATAG TGTAAACTGAAACATGCACATTTTGTACATTGTGCTTTCTTTTGTGGGACATATGCAGTG TGATCCAGTTGTTTTCCATCATTTGGTTGCGCTGACCTAGGAATGTTGGTCATATCAAAC ATTAAAAATGACCACTCTTTTAATTGAAATTAACTTTTAAATGTTTATAGGAGTATGTGC TGTGAAGTGATCTAAAATTTGTAATATTTTTGTCATGAACTGTACTACTCCTAATTATTG TAATGTAATAAAAATAGTTACAGTGACAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_004985 unedited
TATTACACCCGCCCGTTGCCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAA GCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCG CGAATTCGGCACGAGGCGGCGGAGGCAGCAGCGGCGGCGGCAGTGGCGGCGGCGAAGGTG GCGGCGGCTCGGCCAGTACTCCCGGCCCCCGCCATTTCGGACTGGGAGCGAGCGCGGCGC AGGCACTGAAGGCGGCGGCGGGGCCAGAGGCTCAGCGGCTCCCAGGTGCGGGAGAGAGGC CTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGCGTAGGCAAGAGT GCCTTGACGATACAGCTAATTCAGAATCATTTTGTGGACGAATATGATCCAACAATAGAG GATTCCTACAGGAAGCAAGTAGTAATTGATGGAGAAACCTGTCTCTTGGATATTCTCGAC ACAGCAGGTCAAGAGGAGTACAGTGCAATGAGGGACCAGTACATGAGGACTGGGGAGGGC TTTCTTTGTGTATTTGCCATAAATAATACTAAATCATTTGAAGATATTCACCATTATAGA GAACAAATTAAAAGAGTTAAGGACTCTGAAGATGTACCTATGGTCCTAGTAGGAAATAAA TGTGATTTGCCTTCTAGAACAGTAGACACANAACAGGCTCAGGACTTAGCAAGAAGTTAT GGAATTCCTTNTATTGAAACATCAGCAAAGACAAGACAGGGTGTTGATGATGCCTTCTAT ACATTAGNTCGAGAAATTNCGAAACATANAGAANAGATGAGCANAGATGGGTAAAAGAAG AAAAAGAAGTCNAAGACAAGTGTGTAANTATGTAAATACCATTNGTACTTTTNTCTTAAG CATACTAGTACAAGGGGTAATTTTTGTACATTACACTAAATT >OriGene 3' read for NM_004985 unedited
NGGGAAATCTATGNACCGCGCCGCATTCTANGTCGAGTTTTTTTTTTTTTTTTTTGTCAC TGTAACTATTTTTATTACATTACAATAATTAGGAGTAGTACAGTTCATGACAAAAATTTA CAAATTTTAGATCACTTCACAGCACATACTCCTATAAACATTTAAAAGTTAATTTCAATT AAAAGAGTGGTCATTTTTAATGTTTGATATGACCAACATTCCTAGGTCAGCGCAACCAAA TGAATGAAAACAACTGGATCACACTGCATATGTCCCACAAAAGAAAGCACAATGTACAAA ATGTGCATGTTTCAGTTTACACTATACAAAAATAGTTAAAATACATTCCAGGTAAACATG TTACATTAAGAAATAGTACTAGTAAGAAATTGGCACTCAAAGGAAAAATGCAAAAGTATT TTCAACATGAAAACACAAGACAGTGGAATTGAAAACTTTCGGATAAAACACTGTAACCCA GTTAGCTCTGTGGGGGTGTGGGGGGAAGAGATGGGCCCTCAACATTTCTGCAGATAACTT TTTTCCCCTAAATTCATCTAAATTACCTATCATTATCCCAAACAGGCACTTCAAACTATT AAACTAAAACACAGATCTTAATCTAGTTATGACTATTCTTCAAGAACTCATGTGAGTATC TTTCTTTAGAAAGAAAGTTTCATTTTATGACAGCTATTCACGTTCTCAATGCAGAATTCA TGCTATCCAGTATTAACACAGAAAGTACTAAATATAAATTCAGCTTTAAGGTAACTGCTG GGTNTCTAAAAACATTTCTACACAATTATCAAGAAACATTACTTTTTGACAATGGGAATC TTAAATAGTTTTGCTGTCTAAAAAAAATCCCTAAAAAGTTATTACTGGTTGAAGAAAAAT GTTAGA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_004985 |
| Insert Size | 2067 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004985.3, NP_004976.2 |
| RefSeq Size | 5312 bp |
| RefSeq ORF | 567 bp |
| Locus ID | 3845 |
| UniProt ID | P01116 |
| Domains | ras, RAS, RHO, RAB |
| Protein Families | Druggable Genome |
| Protein Pathways | Acute myeloid leukemia, Axon guidance, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Tight junction, VEGF signaling pathway |
| Gene Summary | This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (b) is composed of five exons and lacks exon 4a which the longer transcript variant (a) includes. This predominant variant (b) has a cds that terminates in exon 4b and encodes isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A two-residue nascent-strand steric gate controls synthesis of 2'-O-methyl- and 2'-O-(2-methoxyethyl)-RNA.
,null,
Nature chemistry
,PubMed ID 36229679
[KRAS]
|
|
A modular XNAzyme cleaves long, structured RNAs under physiological conditions and enables allele-specific gene silencing.
,null,
Nature chemistry
,PubMed ID 36064973
[KRAS]
|
|
Structural and functional characterization of a DARPin which inhibits Ras nucleotide exchange
,Guillard, S;Kolasinska-Zwierz, P;Debreczeni, J;Breed, J;Zhang, J;Bery, N;Marwood, R;Tart, J;Overman, R;Stocki, P;Mistry, B;Phillips, C;Rabbitts, T;Jackson, R;Minter, R;,
Nat Commun
,PubMed ID 28706291
[KRAS]
|
|
Differentially expressed nucleolar transforming growth factor-ß1 target (DENTT) exhibits an inhibitory role on tumorigenesis
,Lana E. Kandalaft, Enrique Zudaire, Sergio Portal-Núñez, Frank Cuttitta, and Sonia B. Jakowlew,
Carcinogenesis, Jun 2008; 29: 1282 - 1289.
[KRAS]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201958 | KRAS (Myc-DDK-tagged)-Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b |
CNY 3,600.00 |
|
| RC201958L1 | Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC201958L2 | Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, mGFP tagged |
CNY 6,000.00 |
|
| RC201958L3 | Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, Myc-DDK-tagged |
CNY 6,000.00 |
|
| RC201958L4 | Lenti ORF clone of Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b, mGFP tagged |
CNY 6,000.00 |
|
| RG201958 | KRAS (tGFP-tagged) - Human v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS), transcript variant b |
CNY 5,200.00 |
