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DNA Ligase III (LIG3) (NM_013975) Human Untagged Clone
CAT＃: SC109386
LIG3 (untagged)-Human ligase III, DNA, ATP-dependent (LIG3), nuclear gene encoding mitochondrial protein, transcript variant alpha
CNY 10,552.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | LIG2; LIG3alpha |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_013975 edited
ATTTAAAGAGACAGGCGCTCCAACCGTCGTGGGCTGCCCGCGGCCTGTAATGAGCAAGTT CCGAGGCCTACGGTGAGCGCCGGAGCCGGAGAGGCAGCTATATGTCTTTGGCTTTCAAGA TCTTCTTTCCACAAACCCTCCGTGCACTCAGCCGAAAAGAACTGTGCCTATTCCGAAAAC ATCACTGGCGTGATGTAAGACAATTCAGCCAGTGGTCAGAAACAGATCTGCTTCATGGAC ATCCCCTCTTCCTGAGAAGAAAGCCTGTTCTATCATTCCAGGGAAGCCATCTAAGATCAC GTGCCACCTACCTTGTTTTCTTGCCAGGGTTGCATGTGGGACTCTGCAGTGGCCCCTGTG AGATGGCTGAGCAACGGTTCTGTGTGGACTATGCCAAGCGTGGCACAGCTGGCTGCAAAA AATGCAAGGAAAAGATTGTGAAGGGCGTATGCCGAATTGGCAAAGTGGTGCCCAATCCCT TCTCAGAGTCTGGGGGTGATATGAAAGAGTGGTACCACATTAAATGCATGTTTGAGAAAC TAGAGCGGGCCCGGGCCACCACAAAAAAAATCGAGGACCTCACAGAGCTGGAAGGCTGGG AAGAGCTGGAAGATAATGAGAAGGAACAGATAACCCAGCACATTGCAGATCTGTCTTCTA AGGCAGCAGGTACACCAAAGAAGAAAGCTGTTGTCCAGGCTAAGTTGACAACCACTGGCC AGGTGACTTCTCCAGTGAAAGGCGCCTCATTTGTCACCAGTACCAATCCCCGGAAATTTT CTGGCTTTTCAGCCAAGCCCAACAACTCTGGGGAAGCCCCCTCGAGCCCCACCCCTAAGA GAAGTCTGTCTTCAAGCAAATGTGACCCCAGGCATAAGGACTGTCTGCTACGG:GAGTTT CGAAAGTTATGCGCCATGGTGGCCGATAATCCTAGCTACAACACGAAGACCCAGATCATC CAGGACTTCCTTCGGAAAGGCTCAGCAGGAGATGGTTTCCACGGTGATGTGTACCTAACA GTGAAGCTGCTGCTGCCAGGAGTCATTAAGACTGTTTACAACTTGAACGATAAGCAGATT GTGAAGCTTTTCAGTCGCATTTTTAACTGCAACCCAGATGATATGGCACGGGACCTAGAG CAGGGTGACGTGTCAGAGACAATCAGAGTCTTCTTTGAGCAGAGCAAGTCTTTCCCCCCA GCTGCCAAGAGCCTCCTTACCATCCAGGAAGTGGATGAGTTCCTTCTGCGGCTGTCCAAG CTCACCAAGGAGGATGAGCAGCAACAGGCCCTACAGGACATTGCCTCCAGGTGTACAGCC AATGACCTTAAATGCATCATCAGGTTGATCAAACATGATCTGAAGATGAACTCAGGTGCA AAACATGTGTTAGACGCCCTTGACCCCAATGCCTATGAAGCCTTCAAAGCCTCGCGCAAC CTGCAGGATGTGGTGGAGCGGGTCCTTCACAACGCGCAGGAGGTGGAGAAGGAGCCGGGC CAGAGACGAGCTCTGAGCGTCCAGGCCTCGCTGATGACACCTGTGCAGCCCATGTTGGCG GAGGCCTGCAAGTCCGTTGAGTATGCAATGAAGAAATGTCCCAATGGCATGTTCTCTGAG ATCAAGTACGATGGAGAGCGAGTCCAGGTGCATAAGAATGGAGACCACTTCAGCTACTTC AGCCGCAGTCTCAAGCCCGTCCTTCCTCACAAGGTGGCCCACTTTAAGGACTACATTCCC CAGGCTTTTCCTGGGGGCCACAGCATGATCTTGGATTCTGAAGTGCTTCTGATTGACAAC AAGACAGGCAAACCACTGCCCTTTGGGACTCTGGGAGTACACAAGAAAGCAGCCTTCCAG GATGCTAATGTCTGCCTGTTTGTTTTTGATTGTATCTACTTTAATGATGTCAGCTTGATG GACAGACCTCTGTGTGAGCGGCGGAAGTTTCTTCATGACAACATGGTTGAAATTCCAAAC CGGATCATGTTCTCAGAAATGAAGCGAGTCACAAAAGCTTTGGACTTGGCTGACATGATA ACCCGGGTGATCCAGGAGGGATTGGAGGGGCTGGTGCTGAAGGATGTGAAGGGTACATAT GAGCCTGGGAAGCGGCACTGGCTGAAAGTGAAGAAAGACTATTTGAACGAGGGGGCCATG GCCGACACAGCTGACCTGGTGGTCCTTGGAGCCTTCTATGGGCAAGGGAGCAAAGGCGGC ATGATGTCAATCTTCCTCATGGGCTGCTACGACCCTGGCAGCCAGAAGTGGTGCACAGTC ACCAAGTGTGCAGGAGGCCATGATGATGCCACGCTTGCCCGCCTGCAGAATGAACTAGAC ATGGTGAAGATCAGCAAGGACCCCAGCAAAATACCCAGCTGGTTGAAGGTCAACAAGATC TACTATCCTGACTTCATCGTCCCAGACCCAAAGAAAGCTGCCGTGTGGGAGATCACAGGG GCTGAATTCTCCAAATCGGAGGCTCATACAGCTGACGGGATCTCCATCCGATTCCCTCGC TGCACCCGAATCCGAGATGATAAGGACTGGAAATCTGCCACTAACCTTCCCCAACTCAAG GAACTGTACCAGTTGTCCAAGGAGAAGGCAGACTTCACTGTAGTGGCTGGAGATGAGGGG AGCTCCACTACAGGGGGTAGCAGTGAAGAGAATAAGGGTCCCTCAGGGTCTGCTGTGTCC CGCAAGGCCCCCAGCAAGCCCTCAGCCAGTACCAAGAAAGCAGAAGGGAAGCTGAGTAAC TCCAACAGCAAAGATGGCAACATGCAGACTGCAAAGCCTTCCGCTATGAAGGTGGGGGAG AAGCTGGCCACAAAGTCTTCTCCAGTGAAAGTAGGGGAGAAGCGGAAAGCTGCTGATGAG ACGCTGTGCCAAACAAAGGTATTGCTGGACATCTTCACTGGGGTGCGGCTTTACTTGCCA CCCTCCACACCAGACTTCAGCCGTCTCAGACGCTACTTTGTGGCATTCGACGGGGACCTG GTACAGGAATTTGATATGACTTCAGCCACGCACGTGCTGGGTAGCAGGGACAAGAACCCT GCGGCCCAGCAGGTCTCCCCAGAGTGGATTTGGGCATGTATCCGGAAACGGAGACTGGTA GCTCCCTGCTAGGTTTGCTGTCTTCCCTCTCCCTCAGGCCATACTCTCCTTTACCATACT ACTGGACTGGACTCAGGCTGGAGGCAGATAGACACAGTATAGGGGGAATGGGCTTGCTTC TCCCAAACCCACCAGTTCTCCACTGTCTCTTCTGGACCAGGAATTAGTTGCTGTGGGTGC CACAGCTGAAGTCAGTTTGTCTTGCTGGTTTAAATAGATCTTTCAGAGCTGGGTGCTGGG TTTGCCATCTTTTTGTTTTCTTTGAAAAGCAGCTTAGTTACCCTTTTTATAAATAAAATA TCTTGCAGTAAAAAAAAAAAAAAAAAAA >OriGene 5' read for NM_013975 unedited
TACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGATTTAAAGAGACGGCGCTCCA ACCGTCGTGGGCTGCCCGCGGCCTGTAATGAGCAAGTTCCGAGGCCTACGGTGAGCGCCG GAGCCGGAGAGGCAGCTATATGTCTTTGGCTTTCAAGATCTTCTTTCCACAAACCCTCCG TGCACTCAGCCGAAAAGAACTGTGCCTATTCCGAAAACATCACTGGCGTGATGTAAGACA ATTCAGCCAGTGGTCAGAAACAGATCTGCTTCATGGACATCCCCTCTTCCTGAGAAGAAA GCCTGTTCTATCATTCCAGGGAAGCCATCTAAGATCACGTGCCACCTACCTTGTTTTCTT GCCAGGGTTGCATGTGGGACTCTGCAGTGGCCCCTGTGAGATGGCTGAGCAACGGTTCTG TGTGGACTATGCCAAGCGTGGCACAGCTGGCTGCAAAAAATGCAAGGAAAAGATTGTGAA GGGCGTATGCCGAATTGGCAAAGTGGTGCCCAATCCCTTCTCAGAGTCTGGGGGTGATAT GAAAGAGTGGTACCACATTAAATGCATGTTTGAGAAACTAGAGCGGGCCCGGGCCACCAC AAAAAATATCGAGGACCTCACAGAGCTGGAAGGCTNGGAAGAGCTGGAAAGATATGAGAA GGAACAGANTACCCAGCACATTGCAGATCTGTCTTCTAGGCAGCAGGTACACCAAAGAAG AAAGCTGGTGTCCAGNCTAAAAGTGACACCACTGGNCCAGTGACTTCTCCAGTGAAAGGC GCCTCATTTGTCACCAGTACCAATCCCCGGATATTNTCTGGNCTTTTCAGCCCAGCCCCA TCACTCTGGGGGAATGCCCNCTCGAGCCCACCCCTAAGAAGAAGTCTGTCTTCAGCAAAT GTGACCCCAGCATAAGGACTGCTGCTCACGNAGTTTTCCAAAGTCTGCCCCATGGNGGNC GATATCCTATCTCACACGAGAACCAGATCTCCAGACTTCTTCGAAGCTANAAGGG >OriGene 3' read for NM_013975 unedited
GACCGCGGGCCGCATTTTAGNATCGAGTTTTTTTTTTTTTTTTTTACTGCAAGATATTTT ATTTATAAAAAGGGTAACTAAGCTGCTTTTCAAAGAAAACAAAAAGATGGCAAACCCAGC ACCCAGCTCTGAAAGATCTATTTAAACCAGCAAGACAAACTGACTTCAGCTGTGGCACCC ACAGCAACTAATTCCTGGTCCAGAAGAGACAGTGGAGAACTGGTGGGTTTGGGAGAAGCA AGCCCATTCCCCCTATACTGTGTCTATCTGCCTCCAGCCTGAGTCCAGTCCAGTAGTATG GTAAAGGAGAGTATGGCCTGAGGGAGAGGGAAGACAGCAAACCTAGCAGGGAGCTACCAG TCTCCGTTTCCGGATACATGCCCAAATCCACTCTGGGGAGACCTGCTGGGCCGCAGGGCT CTTGCCCCTGCTACCCAGCACGTGCGTGGCTGAAGTCATATCAAATCCCTGTACCACGCC CCCGTCGAATGCCACAAAGTATCGCCCGACACGGCTGAAGTCTGGTGTGGAGGGTGGTAA GTAAAGCCGCACCCCAGTGAAGATGTCCAGCAATACCTTTGTTTGGCACAGCGTCTCATC ATCAGCTTCTCCGCTTCTCCCCTACTTTCACTGCAGAAGACTTTGTGGCCAGCTTTTCCC CCACCTTCATAGCCGAAGGCTTTGCAGCCTGCATGCCGCCATCCCTCGCTGTTGGAGTTA CCCAACTTCCCCTTTCCTTTCCTTGTCACTGGCTGCGGGCCTGCTTGGGGGCCCCGCCGG ACACAGCACACCCCTGAGGACCCTNACTTTTCTCCACTGGCTACCCCCTGGAGCGCAGCT CCCCTTAATCTCCGCCCCTAACGTGAAGTCCGCCTTCATCCTTGGACAACCGGACCACGC TTCCTCGATCTCGGGCACAGTTACCGTTATACATTCCATCCTTCACATCTCGGAT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_013975 |
| Insert Size | 3510 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_013975.2, NP_039269.2 |
| RefSeq Size | 3701 bp |
| RefSeq ORF | 3030 bp |
| Locus ID | 3980 |
| UniProt ID | P49916 |
| Domains | DNA_ligase, BRCT, zf-PARP |
| Protein Families | Druggable Genome |
| Protein Pathways | Base excision repair |
| Gene Summary | This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha). |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
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Overexpression of DNA ligase III in mitochondria protects cells against oxidative stress and improves mitochondrial DNA base excision repair
,Akbari, M;Keijzers, G;Maynard, S;Scheibye-Knudsen, M;Desler, C;Hickson, ID;Bohr, VA;,
DNA Repair (Amst.)
,PubMed ID 24674627
[LIG3]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC209530 | LIG3 (Myc-DDK-tagged)-Human ligase III, DNA, ATP-dependent (LIG3), nuclear gene encoding mitochondrial protein, transcript variant alpha |
CNY 10,536.00 |
|
| RC209530L1 | Lenti ORF clone of Human ligase III, DNA, ATP-dependent (LIG3), nuclear gene encoding mitochondrial protein, transcript variant alpha, Myc-DDK-tagged |
CNY 12,936.00 |
|
| RC209530L2 | Lenti ORF clone of Human ligase III, DNA, ATP-dependent (LIG3), nuclear gene encoding mitochondrial protein, transcript variant alpha, mGFP tagged |
CNY 7,600.00 |
|
| RC209530L3 | Lenti ORF clone of Human ligase III, DNA, ATP-dependent (LIG3), nuclear gene encoding mitochondrial protein, transcript variant alpha, Myc-DDK-tagged |
CNY 7,600.00 |
|
| RC209530L4 | Lenti ORF clone of Human ligase III, DNA, ATP-dependent (LIG3), nuclear gene encoding mitochondrial protein, transcript variant alpha, mGFP tagged |
CNY 12,936.00 |
|
| RG209530 | LIG3 (tGFP-tagged) - Human ligase III, DNA, ATP-dependent (LIG3), nuclear gene encoding mitochondrial protein, transcript variant alpha |
CNY 12,136.00 |
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