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PCDH1 (NM_002587) Human Untagged Clone
CAT＃: SC109553
PCDH1 (untagged)-Human protocadherin 1 (PCDH1), transcript variant 1
CNY 11,080.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PC42; PCDH42 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC109553 representing NM_002587
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGCGGGGCGGGCGGCCGGCGCTGCCCGGAGGCGGCCCTCCTGATTCTGGGGCCTCCCAGGATGG AGCACCTGAGGCACAGCCCAGGCCCTGGGGGGCAACGGCTACTGCTGCCCTCCATGCTGCTAGCACTGCT GCTCCTGCTGGCTCCATCCCCAGGCCACGCCACTCGGGTAGTGTACAAGGTGCCGGAGGAACAGCCACCC AACACCCTCATTGGGAGCCTCGCAGCCGACTATGGTTTTCCAGATGTGGGGCACCTGTACAAGCTAGAGG TGGGTGCCCCGTACCTTCGCGTGGATGGCAAGACAGGTGACATTTTCACCACCGAGACCTCCATCGACCG TGAGGGGCTCCGTGAATGCCAGAACCAGCTCCCTGGTGATCCCTGCATCCTGGAGTTTGAGGTATCTATC ACAGACCTCGTGCAGAATGGCAGCCCCCGGCTGCTAGAGGGCCAGATAGAAGTACAAGACATCAATGACA ACACACCCAACTTCGCCTCACCAGTCATCACTCTGGCCATCCCTGAGAACACCAACATCGGCTCACTCTT CCCCATCCCGCTGGCTTCAGACCGTGATGCTGGTCCCAACGGTGTGGCATCCTATGAGCTGCAGGCTGGG CCTGAGGCCCAGGAGCTATTTGGGCTGCAGGTGGCAGAGGACCAGGAGGAGAAGCAACCACAGCTCATTG TGATGGGCAACCTGGACCGTGAGCGCTGGGACTCCTATGACCTCACCATCAAGGTGCAGGATGGCGGCAG CCCCCCACGCGCCAGCAGTGCCCTGCTGCGTGTCACCGTGCTTGACACCAATGACAACGCCCCCAAGTTT GAGCGGCCCTCCTATGAGGCCGAACTATCTGAGAATAGCCCCATAGGCCACTCGGTCATCCAGGTGAAGG CCAATGACTCAGACCAAGGTGCCAATGCAGAAATCGAATACACATTCCACCAGGCGCCCGAAGTTGTGAG GCGTCTTCTTCGACTGGACAGGAACACTGGACTTATCACTGTTCAGGGCCCGGTGGACCGTGAGGACCTA AGCACCCTGCGCTTCTCAGTGCTTGCTAAGGACCGAGGCACCAACCCCAAGAGTGCCCGTGCCCAGGTGG TTGTGACCGTGAAGGACATGAATGACAATGCCCCCACCATTGAGATCCGGGGCATAGGGCTAGTGACTCA TCAAGATGGGATGGCTAACATCTCAGAGGATGTGGCAGAGGAGACAGCTGTGGCCCTGGTGCAGGTGTCT GACCGAGATGAGGGAGAGAATGCAGCTGTCACCTGTGTGGTGGCAGGTGATGTGCCCTTCCAGCTGCGCC AGGCCAGTGAGACAGGCAGTGACAGCAAGAAGAAGTATTTCCTGCAGACTACCACCCCGCTAGACTACGA GAAGGTCAAAGACTACACCATTGAGATTGTGGCTGTGGACTCTGGCAACCCCCCACTCTCCAGCACTAAC TCCCTCAAGGTGCAGGTGGTGGACGTCAATGACAACGCACCTGTCTTCACTCAGAGTGTCACTGAGGTCG CCTTCCCGGAAAACAACAAGCCTGGTGAAGTGATTGCTGAGATCACTGCCAGTGATGCTGACTCTGGCTC TAATGCTGAGCTGGTTTACTCTCTGGAGCCTGAGCCGGCTGCTAAGGGCCTCTTCACCATCTCACCCGAG ACTGGAGAGATCCAGGTGAAGACATCTCTGGATCGGGAACAGCGGGAGAGCTATGAGTTGAAGGTGGTGG CAGCTGACCGGGGCAGTCCTAGCCTCCAGGGCACAGCCACTGTCCTTGTCAATGTGCTGGACTGCAATGA CAATGACCCCAAATTTATGCTGAGTGGCTACAACTTCTCAGTGATGGAGAACATGCCAGCACTGAGTCCA GTGGGCATGGTGACTGTCATTGATGGAGACAAGGGGGAGAATGCCCAGGTGCAGCTCTCAGTGGAGCAGG ACAACGGTGACTTTGTTATCCAGAATGGCACAGGCACCATCCTATCCAGCCTGAGCTTTGATCGAGAGCA ACAAAGCACCTACACCTTCCAGCTGAAGGCAGTGGATGGTGGCGTCCCACCTCGCTCAGCTTACGTTGGT GTCACCATCAATGTGCTGGACGAGAATGACAACGCACCCTATATCACTGCCCCTTCTAACACCTCTCACA AGCTGCTGACCCCCCAGACACGTCTTGGTGAGACGGTCAGCCAGGTGGCAGCCGAGGACTTTGACTCTGG TGTCAATGCTGAGCTGATCTACAGCATTGCAGGTGGCAACCCTTATGGACTCTTCCAGATTGGGTCACAT TCAGGTGCCATCACCCTGGAGAAGGAGATTGAGCGGCGCCACCATGGGCTACACCGCCTGGTGGTGAAGG TCAGTGACCGCGGCAAGCCCCCACGCTATGGCACAGCCTTGGTCCATCTTTATGTCAATGAGACTCTGGC CAACCGCACGCTGCTGGAGACCCTCCTGGGCCACAGCCTGGACACGCCGCTGGATATTGACATTGCTGGG GATCCAGAATATGAGCGCTCCAAGCAGCGTGGCAACATTCTCTTTGGTGTGGTGGCTGGTGTGGTGGCCG TGGCCTTGCTCATCGCCCTGGCGGTTCTTGTGCGCTACTGCAGACAGCGGGAGGCCAAAAGTGGTTACCA GGCTGGTAAGAAGGAGACCAAGGACCTGTATGCCCCCAAGCCCAGTGGCAAGGCCTCCAAGGGAAACAAA AGCAAAGGCAAGAAGAGCAAGTCCCCAAAGCCCGTGAAGCCAGTGGAGGACGAGGATGAGGCCGGGCTGC AGAAGTCCCTCAAGTTCAACCTGATGAGCGATGCCCCTGGGGACAGTCCCCGCATCCACCTGCCCCTCAA CTACCCACCAGGCAGCCCTGACCTGGGCCGCCACTATCGCTCTAACTCCCCACTGCCTTCCATCCAGCTG CAGCCCCAGTCACCCTCAGCCTCCAAGAAGCACCAGGTGGTACAGGACCTGCCACCTGCAAACACATTCG TGGGCACCGGGGACACCACGTCCACGGGCTCTGAGCAGTACTCCGACTACAGCTACCGCACCAACCCCCC CAAATACCCCAGCAAGCAGGTAGGCCAGCCCTTTCAGCTCAGCACACCCCAGCCCCTACCCCACCCCTAC CACGGAGCCATCTGGACCGAGGTGTGGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | NotI-NotI |
| ACCN | NM_002587 |
| Insert Size | 4200 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002587.3, NP_002578.2 |
| RefSeq Size | 3851 bp |
| RefSeq ORF | 3183 bp |
| Locus ID | 5097 |
| UniProt ID | Q08174 |
| Domains | CA |
| Protein Families | Transcription Factors, Transmembrane |
| Gene Summary | This gene belongs to the protocadherin subfamily within the cadherin superfamily. The encoded protein is a membrane protein found at cell-cell boundaries. It is involved in neural cell adhesion, suggesting a possible role in neuronal development. The protein includes an extracelllular region, containing 7 cadherin-like domains, a transmembrane region and a C-terminal cytoplasmic region. Cells expressing the protein showed cell aggregation activity. Alternative splicing occurs in this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) lacks two exons and its transcription extends past a splice site that is used in variant 2, resulting in a novel 3' coding region and 3' UTR compared to variant 2. It encodes isoform 1 which is shorter and has a distinct C-terminus, compared to isoform 2. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Protocadherin-1 binds to SMAD3 and suppresses TGFß1-induced gene transcription
,Faura Tellez, G;Vandepoele, K;Brouwer, U;Koning, H;Elderman, RM;Hackett, TL;Willemse, BW;Holloway, J;Van Roy, F;Koppelman, GH;Nawijn, MC;,
Am. J. Physiol. Lung Cell Mol. Physiol.
,PubMed ID 26209277
[PCDH1]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207717 | PCDH1 (Myc-DDK-tagged)-Human protocadherin 1 (PCDH1), transcript variant 1 |
CNY 11,072.00 |
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| RC207717L3 | Lenti ORF clone of Human protocadherin 1 (PCDH1), transcript variant 1, Myc-DDK-tagged |
CNY 12,450.00 |
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| RC207717L4 | Lenti ORF clone of Human protocadherin 1 (PCDH1), transcript variant 1, mGFP tagged |
CNY 12,450.00 |
|
| RG207717 | PCDH1 (tGFP-tagged) - Human protocadherin 1 (PCDH1), transcript variant 1 |
CNY 11,590.00 |
