TRIM45 (NM_025188) Human Untagged Clone

CAT#: SC110258

TRIM45 (untagged)-Human tripartite motif containing 45 (TRIM45), transcript variant 1



  "NM_025188" in other vectors (6)

CNY 4,328.00

CNY 9,310.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TRIM45 mouse monoclonal antibody, clone OTI3G3 (formerly 3G3)
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms RNF99
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC110258 sequence for NM_025188 edited (data generated by NextGen Sequencing)
ATGTCAGAAAACAGAAAACCGCTGCTGGGCTTTGTAAGCAAACTCACTAGTGGGACTGCA
CTTGGGAACTCAGGCAAGACTCACTGCCCCCTGTGCTTGGGGCTTTTCAAAGCCCCCAGG
CTCTTGCCTTGTTTGCATACAGTTTGCACCACGTGTCTGGAGCAGCTGGAGCCCTTCTCA
GTAGTGGACATCCGAGGGGGAGACTCTGACACAAGCTCTGAGGGGTCAATATTCCAGGAA
CTCAAGCCACGAAGTCTGCAGTCGCAGATCGGCATCCTTTGTCCTGTATGTGATGCTCAG
GTGGACCTGCCCATGGGTGGAGTGAAGGCTTTAACCATAGACCACCTGGCCGTGAATGAT
GTGATGCTGGAGAGCCTACGTGGGGAAGGCCAGGGCCTGGTGTGTGACCTGTGCAACGAC
AGGGAAGTAGAGAAGAGGTGTCAGACCTGCAAAGCCAACCTCTGCCACTTCTGCTGCCAG
GCTCATAGGCGGCAGAAGAAAACGACTTACCACACCATGGTGGACCTAAAAGACTTGAAA
GGCTACAGCCGGATTGGGAAGCCCATCCTGTGTCCTGTTCACCCTGCAGAGGAACTGAGG
CTGTTCTGTGAGTTCTGTGACCGGCCCGTGTGCCAGGATTGTGTGGTGGGGGAGCATCGG
GAACACCCCTGTGACTTCACCAGCAATGTCATCCACAAGCATGGGGACTCTGTGTGGGAG
CTCCTCAAAGGTACTCAGCCCCACGTGGAGGCCCTGGAGGAAGCCCTGGCTCAGATCCAC
ATAATAAACAGTGCCCTCCAGAAGCGAGTGGAGGCAGTGGCAGCTGATGTCCGGACATTC
TCGGAGGGCTACATTAAGGCCATTGAGGAGCATCGGGACAAGCTGCTGAAGCAGCTGGAA
GACATACGGGCCCAGAAGGAAAATTCCCTGCAGCTGCAGAAGGCCCAGCTGGAACAGTTA
CTGGCAGACATGCGGACTGGAGTGGAGTTCACCGAGCACTTGCTGACCAGCGGCTCAGAC
TTGGAGATCCTCATCACCAAGAGGGTGGTGGTAGAACGGCTCAGGAAGCTGAACAAAGTT
CAATATAGCACCCGTCCTGGAGTAAATGATAAGATACGCTTCTGTCCTCAGGAGAAAGCA
GGCCAGTGCCGTGGCTATGAAATTTATGGTACGATTAATACCAAAGAGGTTGATCCAGCC
AAATGTGTCCTACAAGGAGAAGACCTCCACAGAGCCCGGGAGAAACAGACGGCCTCTTTC
ACCCTGCTTTGTAAGGATGCCGCAGGAGAAATCATGGGCAGGGGAGGAGACAACGTTCAA
GTTGCCGTTGTCCCTAAAGATAAGAAAGACAGCCCAGTCAGAACAATGGTCCAGGATAAC
AAGGATGGGACATACTACATTTCCTACACCCCCAAGGAACCTGGCGTCTATACTGTGTGG
GTCTGCATCAAAGAACAGCATGTGCAGGGCTCGCCATTCACTGTGACGGTGAGGAGAAAG
CACCGCCCACACTCAGGCGTGTTTCACTGCTGCACCTTCTGCTCCAGCGGGGGCCAGAAA
ACCGCTCGCTGCGCCTGTGGAGGCACCATGCCAGGTGGGTACCTAGGCTGTGGCCATGGA
CACAAAGGCCACCCAGGTCATCCCCACTGGTCATGCTGTGGAAAATTTAATGAGAAATCT
GAATGCACATGGACAGGTGGGCAGAGCGCACCGAGGAGTCTACTTAGGACTGTGGCTCTC
TGA

Clone variation with respect to NM_025188.3
1487 t=>c
>OriGene 5' read for NM_025188 unedited
GTTAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCGGGAGAGGC
GGTGGACACCGAGAAGCCCGCCGGCGGCTTGCAATTCCCTCACCCGGCGCGCGCCTTTCG
CAGAGGGAAGGAGCAAGAGGGCCCCTACCTCATCGTGCGCGGGTGGGGTCGGCGCTTGTC
GCGTGTGGCGCGCAGTGGGGCGGGCGGCGGGAGGGGGGTGGCAGTGGAGGGAGCGAGAGG
TGCAGGGGTGACTTTGTTGGCAGCAGGACTAGCTGGAGAGCTAGACCTGGAAGCGCATCC
GGGGAGGACTTGCGGGGCAGAGGAGGGCGTGGGCGTGTCTGGTATGGGATGCAGTGGAAA
GGAGGGGGCCCTCCTGAGTAGATCTGTGGGTGATTCCTTCGAGGACGCCTCGTCTTCCCG
TCTGCCCTTTTATTTGTCAGCGAGGGAGTCCCCATGGTCTCTGTTCAAGTTCTGGAAACT
TTCTCTTTGGGTGGGCTTAATCACCTGCTACTAAATCGTAGAACTGCCCAGGGCCCTTTC
TAATATTGGTCACAAACGTGAGGAGTATGTCAGAAAACAGAAAACCGCTGCTGGGCTTTG
TAAGCAAACTCACTAGTGGGACTGCACTTGGGAACTCANGCAAGACTCACTGCCCCCTGT
GCTTGNNGCTTTTCAAGCCCCCAGGCTCTTGCCTTGTTTGCATACAGTTTGCACCACGTG
TCTGGAGCAGCTGGAGCCCTTCTCAGTAGTGGACATCCGAGGGGNAGACTCTGACACAAG
CTCTNGAGGGTCAATATTCCAGGAAACTCAGCCACGAAGTCTGCAGTCGCAGATCGGCAT
CCTTTGTCCTGTATGTGATGCTCNAGTGGACCTGCCCATGGGTGGAGTGAAGGCTTAACC
ATAGACCACCTGGCCGGC
>OriGene 3' read for NM_025188 unedited
GGCACTTTGTTTTTAATTCTATCAGTCTCTTTAGATGAACAAAGTCTGGGTCCTCTGGAA
ATCTAGTGGTGCTGCCTGCAGCTTTAAAAGGCTGAGCACAAACCCATCAGAGAGCCACAG
TCCTAAGTAGACTCCTCGGTGCGCTCTGCCCACCTGTCCATGTGCATTCAGATTTCTCAT
TAAATTTTCCACAGCATGACCAGTGGGGATGACCTGGGTGGCCTTTGTGTCCATGGCCAC
AGCCTAGGTACCCACCTGGCATGGTGCCTCCACAGGCGCAGCGAGCGGTTTTCTGGCCCC
CGCTGGAGCAGAAGGTGCAGCAGTGAAACACGCCTGAGTGTGGGCGGTGCTTTCTCCTCA
CCGTCACAGTGAATGGCGAGCCCTGCACATGCTGTTCTTTGATGCAGACCCACACAGTAT
AGACGCCAGGTTCCTTGGGGGTGTAGGAAATGTAGTATGTCCCATCCTTGTTATCCTGGA
CCATTGTTCTGACTGGGCTGTCTTTCTTATCTTTAGGGACAACGGCAACTTGAACGTTGT
CTCCTCCCCTGCCCATGATTTCTCCTGCGGCATCCTTACAAAGCAGGGTGAAAGAGGCCG
TCTGTTTCTCCCGGGCTCTGTGGAGGTCTTCTCCTTGTAGGACACATTTGGCTGGATCAA
CCTCTTTGGTATTAATCGTACCATAAATTTCATAGCCACGGCACTGGCCTGCTTTCTCCT
GAGGACAGAAGCGTATCTTATCATTTACTCCAGGACGGGTGCTATATTGAACTTTGTTCA
GCTTCCTGAGCCGTTCTACCACCACCCTCTTGGTGATGAGGATCTNCAAGTCTGAGCCGC
TGGTCAGCAAGTGCTCGGTGAACTNCACTNCAGTCCGCATGTCTGCCAGTAACTGGTCCA
GCTGGGCCTTCTGCAGCTGCAGGGAATTTNCTTCTGGGNCCGTATGTCTTCCAGCTGCTT
CAGCAGCTTGTCCCGAGCTNCTCATGGGCCTAATGTAGCCCTNCGAGATGTNCGGCATCA
GCTGCCTGNCTCCATCGN
Restriction Sites NotI-NotI     
ACCN NM_025188
Insert Size 2680 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_025188.2, NP_079464.1
RefSeq Size 3584 bp
RefSeq ORF 1743 bp
Locus ID 80263
UniProt ID Q9H8W5
Domains zf-B_box, Filamin, RING, BBC
Gene Summary This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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