Ceramide synthase 2 (CERS2) (NM_013384) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	L3, SP260, TMSG1, MGC987, FLJ10243
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC110410 sequence for NM_013384 edited (data generated by NextGen Sequencing) ATGGCCGTCATTGTGGATAAACCCTGGTTCTATGACATGAAGAAAGTTTGGGAGGGATAT CCCATACAGAGCACTATCCCTTCCCAGTATTGGTACTACATGATTGAACTTTCCTTCTAC TGGTCCCTGCTCTTCAGCATTGCCTCTGATGTCAAGCGAAAGGATTTCAAGGAACAGATC ATCCACCATGTGGCCACCATCATTCTCATCAGCTTTTCCTGGTTTGCCAATTACATCCGA GCTGGGACTCTAATCATGGCTCTGCATGACTCTTCCGATTACCTGCTGGAGTCAGCCAAG ATGTTTAACTACGCGGGATGGAAGAACACCTGCAACAACATCTTCATCGTCTTCGCCATT GTTTTTATCATCACCCGACTGGTCATCCTGCCCTTCTGGATCCTGCATTGCACCCTGGTG TACCCACTGGAGCTCTATCCTGCCTTCTTTGGCTATTACTTCTTCAATTCCATGATGGGA GTTCTACAGCTGCTGCATATCTTCTGGGCCTACCTCATTTTGCGCATGGCCCACAAGTTC ATAACTGGAAAGCTGGTAGAAGATGAACGCAGTGACCGGGAAGAAACAGAGAGCTCAGAG GGGGAGGAGGCTGCAGCTGGGGGAGGAGCAAAGAGCCGGCCCCTAGCCAATGGCCACCCC ATCCTCAATAACAACCATCGTAAGAATGACTGA Clone variation with respect to NM_013384.3 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_013384 unedited CTCGGTTNGAAACGATTTATGGAGGCGGCAGACCCATTCCCACGAGGGCCCAGTCTTTCT CAGAGTTCCGAGAAGCCAGCTGGAGATTCACATTTTACCTGATTGCCTTCATTGCCGGCA TGGCCGTCATTGTGGATAAACCCTGGTTCTATGACATGTAACAAAGTTTGGGAGGGATAT CCCATACAGAGCACTATCCCTTCCCAGATGGGTACTACGTGATTGAACTTTCCTTCTACT GGTCCCTGCTCTTCAACATTGCCTCTGATGTCAAGCGAAAGGATTTTAAGGAACAGATCA TCCACCATGTGGCCACCATCATTCTCATCAGCTTTTCCTGGTTTGCCAATTACATCCGAG CTGGGACTCTAATCATGGCTCTGCATGACTCTTCCGATTACCTGCTGGAGTCAGCCAAGA TGTTTAACTACGCGGGATGGAAGAACACCTGCAACAACATCTTCATCGTCTTCGCCATTG TTTTTATCATCACCCGACTGGTCATCCTGCCCTTCTGGATCCTGCATTGCACCCTGGTGT ACCCACTGGAGCTCTATCCTGCCTTCTTTGGCTATTACTTCTTCAATTCCATGATGGGAG TTCTACAGCTGCTGCATATCTTCTGGGCCTACCTCATTTTGCGCATGGCCCACAAGTTCA TAACTGGAAAGCTGGTAGAAGATGAACGCAGTGACCGGGAAGAAACAGACAGCTCATAGG GGGATGAAGCTGCAGCTGGCGGAGGAGCATAGAGCCGGCCCCTAGCCAATGGCCACCCCA TCCTCAATAACACCCTCGTAGAATGACTGAACCATTATTCCCGCTGCCTCCCAGATATTG CATAAAGCCAGGAACTACCTGCTCCCTGGGCTAAGGGTCACTTTAAGCTCTGGG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_013384 unedited TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTAAAAAAAAACTCTTTTCGTTTATTTAGTTGATCCCCCTTTTCAA TTCCTAATAGTCTGACCCTTATTGCCATGTTTTATTTAATATTTTTTATTTAATCTTTTA ATTTTAAAAAAAAACCCATTAACAGTACTTTTTGGTCTAAAAGGGCCCCTTTGCTGAAAT GCTAGGGGCTACCCGTAATTTTGGTTTTAAAACCAAAACCCCAAATTTTTAATAAATAAA AATTAAAATTAGTTGCCATTTTACTCCAAACCAGTTAGCCTAACTGAAAAAAAAAGGGAA GGGGGAAAAGGCCAAAAAAAGGAGGCAGTCAAATTTTAAACCTGTCGCTACAGGGACAGC TGAAAAAAGTACCCCTAAAAAAGATCTTCCGAGCAGTCCCCAGTACAGCCCCCATTTTTT GGCAAAGGTAGGGTAAGGGTTATGTGCACCCTCCTCCTACCCTCAATTAATTTGGGCCAT AAAGGGAGAAAGTTAAAAGCTCAGCTTTGGTTTCTGGCCCCAAGTTAGGGAGCTTAAAAA GGTTAGCCTTGGTCCAGCTTTTGCAGAATGAGGCCCACAGAGGGGACAATAAGGCCCAAC CCTGGCTCTGGAGGTCAGGNAGTCAAAGCCAAACAGTTGGGGCCCAAATTCTCAAAATAT GGGATGTGGATTTGTACCCCTTCCCACAGAGAGCTGGGGGAGGCCCTTCAAACCATAAAA AGATGATTGGTCCGGTTAGGAGGAAATACGACCGTCCCCCTTTACAGAAATAACACAGTC CCCTTACCTGGGGAAAGCCGGTTAAAATTTGTGTTAGAGCCCCTGCGGACACCCAGGAGG GAGGATGAAAAACTTTCCTTTATTTTCTCTTTCCCAAGATTTAAGGGCCCTTATCCCAGG AACAGGTTTTCTCTGCTTACT
Restriction Sites	NotI-NotI
ACCN	NM_013384
Insert Size	1770 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013384.3, NP_037516.3
RefSeq Size	2178 bp
RefSeq ORF	2178 bp
Locus ID	29956
Domains	LAG1, TLC
Protein Families	Transcription Factors, Transmembrane
Gene Summary	This gene encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (3) lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter isoform (2) that has a shorter N-terminus compared to isoform 1.

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