P4HA1 (NM_000917) Human Untagged Clone
CAT#: SC110967
P4HA1 (untagged)-Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 1
CNY 3,984.00
CNY 8,550.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | P4HA |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_000917, the custom clone sequence may differ by one or more nucleotides
ATGATCTGGTATATATTAATTATAGGAATTCTGCTTCCCCAGTCTTTGGCTCATCCAGGCTTTTTTACTT CAATTGGTCAGATGACTGATTTGATCCATACTGAGAAAGATCTGGTGACTTCTCTGAAAGATTATATTAA GGCAGAAGAGGACAAGTTAGAACAAATAAAAAAATGGGCAGAGAAGTTAGATCGGCTAACTAGTACAGCG ACAAAAGATCCAGAAGGATTTGTTGGGCATCCAGTAAATGCATTCAAATTAATGAAACGTCTGAATACTG AGTGGAGTGAGTTGGAGAATCTGGTCCTTAAGGATATGTCAGATGGCTTTATCTCTAACCTAACCATTCA GAGACAGTACTTTCCTAATGATGAAGATCAGGTTGGGGCAGCCAAAGCTCTGTTACGTCTCCAGGATACC TACAATTTGGATACAGATACCATCTCAAAGGGTAATCTTCCAGGAGTGAAACACAAATCTTTTCTAACGG CTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCATACGGAACTGTGGAT GGAACAAGCCCTAAGGCAACTGGATGAAGGCGAGATTTCTACCATAGATAAAGTCTCTGTTCTAGATTAT TTGAGCTATGCGGTATATCAGCAGGGAGACCTGGATAAGGCACTTTTGCTCACAAAGAAGCTTCTTGAAC TAGATCCTGAACATCAGAGAGCTAATGGTAACTTAAAATATTTTGAGTATATAATGGCTAAAGAAAAAGA TGTCAATAAGTCTGCTTCAGATGACCAATCTGATCAGAAAACTACACCAAAGAAAAAAGGGGTTGCTGTG GATTACCTGCCAGAGAGACAGAAGTACGAAATGCTGTGCCGTGGGGAGGGTATCAAAATGACCCCTCGGA GACAGAAAAAACTCTTTTGCCGCTACCATGATGGAAACCGTAATCCTAAATTTATTCTGGCTCCAGCTAA ACAGGAGGATGAATGGGACAAGCCTCGTATTATTCGCTTCCATGATATTATTTCTGATGCAGAAATTGAA ATCGTCAAAGACCTAGCAAAACCAAGGCTGAGCCGAGCTACAGTACATGACCCTGAGACTGGAAAATTGA CCACAGCACAGTACAGAGTATCTAAGAGTGCCTGGCTCTCTGGCTATGAAAATCCTGTGGTGTCTCGAAT TAATATGAGAATACAAGATCTAACAGGACTAGATGTTTCCACAGCAGAGGAATTACAGGTAGCAAATTAT GGAGTTGGAGGACAGTATGAACCCCATTTTGACTTTGCACGGAAAGATGAGCCAGATGCTTTCAAAGAGC TGGGGACAGGAAATAGAATTGCTACATGGCTGTTTTATATGAGTGATGTGTCTGCAGGAGGAGCCACTGT TTTTCCTGAAGTTGGAGCTAGTGTTTGGCCCAAAAAAGGAACTGCTGTTTTCTGGTATAATCTGTTTGCC AGTGGAGAAGGAGATTATAGTACACGGCATGCAGCCTGTCCAGTGCTAGTTGGCAACAAATGGGTATCCA ATAAATGGCTCCATGAACGTGGACAAGAATTTCGAAGACCTTGTACGTTGTCAGAATTGGAATGA >OriGene 5' read for NM_000917 unedited
GTTCACCATTATGTAATACGACTTACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGGC GGGCTGATGGTAGGAAGTAGCCGCTCCGAGTGGAGGCGACTGGGGGCTGAAGAGCGCGCC GCCCTCTCGTCCCACTTTCCAGGTGTGTGATCCTGTAAAATTAAATCTTCCAAGATGATC TGGTATATATTAATTATAGGAATTCTGCTTCCCCAGTCTTTGGCTCATCCAGGCTTTTTT ACTTCAATTGGTCAGATGACTGATTTGATCCATACTGAGAAAGATCTGGTGACTTCTCTT GAAAGATTATATTAAGGCAGAAGAGGACAAGTTAGAACAAATAAAAAAATGGGCAGAGAA GTTAGATCGGCTAACTAGTACAGCGACAAAAGATCCAGAAGGATTTGTTGGGCATCCAGT AAATGCATTCAAATTAATGAAACGTCTGAATACTGAGTGGAGTGAGTTGGAGAATCTGGT CCTTAAGGATATGTCAGATGGCTTTATCTCTAACCTAACCATTCAGAGACAGTACTTTCC TAATGATGAAGATCAGGTTGGGGCAGCCAAAGCTCTGTTACGTCTCCAGGATACCTACAA TTTGGATACAGATACCATCTCAAAGGGTAATCTTCCAGGAGTGAAACACANATCTTTTCT AACGGCTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCA TACGGAACTGTGGATGGAACAAGCCCTAANGCAACTGGATGAANGCGAGATTTCTACCAT AGATNAAGTCTCTGTTCTAGATTATTTGAGCTATGCCGTATATCAGCAGGGAGACCTGNN ATANGCACTTTTGCTCACAAANAAGCTTCTTGCACTAGACCCTGNACATCAGAGAGCTCA TGGTAACCTTAAATATTTTGAGTATATATGGCTAAN >OriGene 3' read for NM_000917 unedited
GACCGCGGACTCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TTTTTTTTTTTTTTTTTTTTACTAAAAAGATTTAATACCATAAATCGGGCTTTGTTGCCA CAACCCTTTTCAAAATTTTAAAAAAACCTCTTTTTTGGCTTTTTAAAAAAAAAACTTTTA AAAAAAATAAATTCCCTCATCACTGAAAGGACTTGTACTTTTTTAAACTTCCAGTCTCCT AAGGCCCATTTTTTAATCAAAACTCCAATTTTACCCCCCTGCTTGTACAGGAATAAAAGG CAGGGTTTTTCCCCTAAAAAAAACAGCAAAATTCCTGAACAAAATTTTTTGTTTTTTTAA AAAAGGGATAAAAAACCCGGCTAAGGGGGGACCTTTTTATTTAAAAAGGCCCACTGTTGT TTTGCCGGACTTTCCGTTAAAAAAAAAAAAAAATTCGAATTATTTTATCTCAAGTCCCGT TTAATTTCCCCCCAGGCTAGCCTTAAAAAAAGAGAGCTGGGGTGTGGAAAATACAGTTCT CTTTTTTTAAAAAAGACTTGGACTGGAAAAAACCCCCTTAAAAAAATTTTGAAAGGGTAA CTCCATGGGACCCCTTTTGTGGCCCTGCTTTTTTACCACCTCTTCAAACCCAGAACCCAG ACTGTCACTCCTGGGGTACAAAAAACACCTAGGCACATTTGTAAGAGGTTTTGGTTTCTT TTCCACACGCCCTTTTTTGTTTTACCACCCGCCCCCTGTGAAGTGTGCGAATACCCACAC CTGGTTCTCATTTTTTTTTTTTCTCCACCACAAATTTTTTAAAATTAAAAACCTCCCCCT TTAAACCTTGGCCTCGTGTTTTGTGATACCCCCAATACTTTCAGGTGTTTTGGCTTCTGA GCACCCCGCGGTCCTTTTAAAACCCCCCCAGTTTCATATGTCCCCAAAG |
Restriction Sites | NotI-NotI |
ACCN | NM_000917 |
Insert Size | 3160 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000917.2, NP_000908.2 |
RefSeq Size | 2752 bp |
RefSeq ORF | 1605 bp |
Locus ID | 5033 |
UniProt ID | P13674 |
Domains | 2OG-FeII_Oxy, P4Hc |
Protein Families | Druggable Genome, P450 |
Protein Pathways | Arginine and proline metabolism, Metabolic pathways |
Gene Summary | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC223831 | P4HA1 (Myc-DDK-tagged)-Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 1 |
CNY 3,976.00 |
|
RC223831L1 | Lenti ORF clone of Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 1, Myc-DDK-tagged |
CNY 6,376.00 |
|
RC223831L2 | Lenti ORF clone of Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC223831L3 | Lenti ORF clone of Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 1, Myc-DDK-tagged |
CNY 6,376.00 |
|
RC223831L4 | Lenti ORF clone of Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 1, mGFP tagged |
CNY 6,376.00 |
|
RG223831 | P4HA1 (tGFP-tagged) - Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 1 |
CNY 5,576.00 |