P4HA1 (NM_000917) Human Untagged Clone

CAT#: SC110967

P4HA1 (untagged)-Human prolyl 4-hydroxylase, alpha polypeptide I (P4HA1), transcript variant 1



  "NM_000917" in other vectors (6)

CNY 3,984.00

CNY 8,550.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
P4HA1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms P4HA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_000917, the custom clone sequence may differ by one or more nucleotides


ATGATCTGGTATATATTAATTATAGGAATTCTGCTTCCCCAGTCTTTGGCTCATCCAGGCTTTTTTACTT
CAATTGGTCAGATGACTGATTTGATCCATACTGAGAAAGATCTGGTGACTTCTCTGAAAGATTATATTAA
GGCAGAAGAGGACAAGTTAGAACAAATAAAAAAATGGGCAGAGAAGTTAGATCGGCTAACTAGTACAGCG
ACAAAAGATCCAGAAGGATTTGTTGGGCATCCAGTAAATGCATTCAAATTAATGAAACGTCTGAATACTG
AGTGGAGTGAGTTGGAGAATCTGGTCCTTAAGGATATGTCAGATGGCTTTATCTCTAACCTAACCATTCA
GAGACAGTACTTTCCTAATGATGAAGATCAGGTTGGGGCAGCCAAAGCTCTGTTACGTCTCCAGGATACC
TACAATTTGGATACAGATACCATCTCAAAGGGTAATCTTCCAGGAGTGAAACACAAATCTTTTCTAACGG
CTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCATACGGAACTGTGGAT
GGAACAAGCCCTAAGGCAACTGGATGAAGGCGAGATTTCTACCATAGATAAAGTCTCTGTTCTAGATTAT
TTGAGCTATGCGGTATATCAGCAGGGAGACCTGGATAAGGCACTTTTGCTCACAAAGAAGCTTCTTGAAC
TAGATCCTGAACATCAGAGAGCTAATGGTAACTTAAAATATTTTGAGTATATAATGGCTAAAGAAAAAGA
TGTCAATAAGTCTGCTTCAGATGACCAATCTGATCAGAAAACTACACCAAAGAAAAAAGGGGTTGCTGTG
GATTACCTGCCAGAGAGACAGAAGTACGAAATGCTGTGCCGTGGGGAGGGTATCAAAATGACCCCTCGGA
GACAGAAAAAACTCTTTTGCCGCTACCATGATGGAAACCGTAATCCTAAATTTATTCTGGCTCCAGCTAA
ACAGGAGGATGAATGGGACAAGCCTCGTATTATTCGCTTCCATGATATTATTTCTGATGCAGAAATTGAA
ATCGTCAAAGACCTAGCAAAACCAAGGCTGAGCCGAGCTACAGTACATGACCCTGAGACTGGAAAATTGA
CCACAGCACAGTACAGAGTATCTAAGAGTGCCTGGCTCTCTGGCTATGAAAATCCTGTGGTGTCTCGAAT
TAATATGAGAATACAAGATCTAACAGGACTAGATGTTTCCACAGCAGAGGAATTACAGGTAGCAAATTAT
GGAGTTGGAGGACAGTATGAACCCCATTTTGACTTTGCACGGAAAGATGAGCCAGATGCTTTCAAAGAGC
TGGGGACAGGAAATAGAATTGCTACATGGCTGTTTTATATGAGTGATGTGTCTGCAGGAGGAGCCACTGT
TTTTCCTGAAGTTGGAGCTAGTGTTTGGCCCAAAAAAGGAACTGCTGTTTTCTGGTATAATCTGTTTGCC
AGTGGAGAAGGAGATTATAGTACACGGCATGCAGCCTGTCCAGTGCTAGTTGGCAACAAATGGGTATCCA
ATAAATGGCTCCATGAACGTGGACAAGAATTTCGAAGACCTTGTACGTTGTCAGAATTGGAATGA


>OriGene 5' read for NM_000917 unedited
GTTCACCATTATGTAATACGACTTACTATAGGGNNCGGCCGCGCAATTCGGCACGAGGGC
GGGCTGATGGTAGGAAGTAGCCGCTCCGAGTGGAGGCGACTGGGGGCTGAAGAGCGCGCC
GCCCTCTCGTCCCACTTTCCAGGTGTGTGATCCTGTAAAATTAAATCTTCCAAGATGATC
TGGTATATATTAATTATAGGAATTCTGCTTCCCCAGTCTTTGGCTCATCCAGGCTTTTTT
ACTTCAATTGGTCAGATGACTGATTTGATCCATACTGAGAAAGATCTGGTGACTTCTCTT
GAAAGATTATATTAAGGCAGAAGAGGACAAGTTAGAACAAATAAAAAAATGGGCAGAGAA
GTTAGATCGGCTAACTAGTACAGCGACAAAAGATCCAGAAGGATTTGTTGGGCATCCAGT
AAATGCATTCAAATTAATGAAACGTCTGAATACTGAGTGGAGTGAGTTGGAGAATCTGGT
CCTTAAGGATATGTCAGATGGCTTTATCTCTAACCTAACCATTCAGAGACAGTACTTTCC
TAATGATGAAGATCAGGTTGGGGCAGCCAAAGCTCTGTTACGTCTCCAGGATACCTACAA
TTTGGATACAGATACCATCTCAAAGGGTAATCTTCCAGGAGTGAAACACANATCTTTTCT
AACGGCTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCA
TACGGAACTGTGGATGGAACAAGCCCTAANGCAACTGGATGAANGCGAGATTTCTACCAT
AGATNAAGTCTCTGTTCTAGATTATTTGAGCTATGCCGTATATCAGCAGGGAGACCTGNN
ATANGCACTTTTGCTCACAAANAAGCTTCTTGCACTAGACCCTGNACATCAGAGAGCTCA
TGGTAACCTTAAATATTTTGAGTATATATGGCTAAN
>OriGene 3' read for NM_000917 unedited
GACCGCGGACTCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTACTAAAAAGATTTAATACCATAAATCGGGCTTTGTTGCCA
CAACCCTTTTCAAAATTTTAAAAAAACCTCTTTTTTGGCTTTTTAAAAAAAAAACTTTTA
AAAAAAATAAATTCCCTCATCACTGAAAGGACTTGTACTTTTTTAAACTTCCAGTCTCCT
AAGGCCCATTTTTTAATCAAAACTCCAATTTTACCCCCCTGCTTGTACAGGAATAAAAGG
CAGGGTTTTTCCCCTAAAAAAAACAGCAAAATTCCTGAACAAAATTTTTTGTTTTTTTAA
AAAAGGGATAAAAAACCCGGCTAAGGGGGGACCTTTTTATTTAAAAAGGCCCACTGTTGT
TTTGCCGGACTTTCCGTTAAAAAAAAAAAAAAATTCGAATTATTTTATCTCAAGTCCCGT
TTAATTTCCCCCCAGGCTAGCCTTAAAAAAAGAGAGCTGGGGTGTGGAAAATACAGTTCT
CTTTTTTTAAAAAAGACTTGGACTGGAAAAAACCCCCTTAAAAAAATTTTGAAAGGGTAA
CTCCATGGGACCCCTTTTGTGGCCCTGCTTTTTTACCACCTCTTCAAACCCAGAACCCAG
ACTGTCACTCCTGGGGTACAAAAAACACCTAGGCACATTTGTAAGAGGTTTTGGTTTCTT
TTCCACACGCCCTTTTTTGTTTTACCACCCGCCCCCTGTGAAGTGTGCGAATACCCACAC
CTGGTTCTCATTTTTTTTTTTTCTCCACCACAAATTTTTTAAAATTAAAAACCTCCCCCT
TTAAACCTTGGCCTCGTGTTTTGTGATACCCCCAATACTTTCAGGTGTTTTGGCTTCTGA
GCACCCCGCGGTCCTTTTAAAACCCCCCCAGTTTCATATGTCCCCAAAG
Restriction Sites NotI-NotI     
ACCN NM_000917
Insert Size 3160 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000917.2, NP_000908.2
RefSeq Size 2752 bp
RefSeq ORF 1605 bp
Locus ID 5033
UniProt ID P13674
Domains 2OG-FeII_Oxy, P4Hc
Protein Families Druggable Genome, P450
Protein Pathways Arginine and proline metabolism, Metabolic pathways
Gene Summary This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (1) encodes isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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