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Vimentin (VIM) (NM_003380) Human Untagged Clone
CNY 3,656.00
| Cited in 4 publications. |
Product images
CNY 1,999.00
CNY 2,700.00
热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC111054 sequence for NM_003380 edited (data generated by NextGen Sequencing)
ATGTCCACCAGGTCCGTGTCCTCGTCCTCCTACCGCAGGATGTTCGGCGGCCCGGGCACC GCGAGCCGGCCGAGCTCCAGCCGGAGCTACGTGACTACGTCCACCCGCACCTACAGCCTG GGCAGCGCGCTGCGCCCCAGCACCAGCCGCAGCCTCTACGCCTCGTCCCCGGGCGGCGTG TATGCCACGCGCTCCTCTGCCGTGCGCCTGCGGAGCAGCGTGCCCGGGGTGCGGCTCCTG CAGGACTCGGTGGACTTCTCGCTGGCCGACGCCATCAACACCGAGTTCAAGAACACCCGC ACCAACGAGAAGGTGGAGCTGCAGGAGCTGAATGACCGCTTCGCCAACTACATCGACAAG GTGCGCTTCCTGGAGCAGCAGAATAAGATCCTGCTGGCCGAGCTCGAGCAGCTCAAGGGC CAAGGCAAGTCGCGCCTGGGGGACCTCTACGAGGAGGAGATGCGGGAGCTGCGCCGGCAG GTGGACCAGCTAACCAACGACAAAGCCCGCGTCGAGGTGGAGCGCGACAACCTGGCCGAG GACATCATGCGCCTCCGGGAGAAATTGCAGGAGGAGATGCTTCAGAGAGAGGAAGCCGAA AACACCCTGCAATCTTTCAGACAGGATGTTGACAATGCGTCTCTGGCACGTCTTGACCTT GAACGCAAAGTGGAATCTTTGCAAGAAGAGATTGCCTTTTTGAAGAAACTCCACGAAGAG GAAATCCAGGAGCTGCAGGCTCAGATTCAGGAACAGCATGTCCAAATCGATGTGGATGTT TCCAAGCCTGACCTCACGGCTGCCCTGCGTGACGTACGTCAGCAATATGAAAGTGTGGCT GCCAAGAACCTGCAGGAGGCAGAAGAATGGTACAAATCCAAGTTTGCTGACCTCTCTGAG GCTGCCAACCGGAACAATGACGCCCTGCGCCAGGCAAAGCAGGAGTCCACTGAGTACCGG AGACAGGTGCAGTCCCTCACCTGTGAAGTGGATGCCCTTAAAGGAACCAATGAGTCCCTG GAACGCCAGATGCGTGAAATGGAAGAGAACTTTGCCGTTGAAGCTGCTAACTACCAAGAC ACTATTGGCCGCCTGCAGGATGAGATTCAGAATATGAAGGAGGAAATGGCTCGTCACCTT CGTGAATACCAAGACCTGCTCAATGTTAAGATGGCCCTTGACATTGAGATTGCCACCTAC AGGAAGCTGCTGGAAGGCGAGGAGAGCAGGATTTCTCTGCCTCTTCCAAACTTTTCCTCC CTGAACCTGAGGGAAACTAATCTGGATTCACTCCCTCTGGTTGATACCCACTCAAAAAGG ACACTTCTGATTAAGACGGTTGAAACTAGAGATGGACAGGTTATCAACGAAACTTCTCAG CATCACGATGACCTTGAATAA Clone variation with respect to NM_003380.3 >OriGene 5' read for NM_003380 unedited
GGTTTAGGATTTGTAACCGACTTACTATAGGGNCGGCACGCGCAATTCGGCACGAGGCTC CGAGGTCCCCGCGCCAGAGACGCAGCCGCGCTCCCACCACCCACACCCACCGCGCCCTCG TTCGCCTCTTCTCCGGGAGCCAGTCCGCGCCACCGCCGCCGCCCAGGCCATCGCCACCCT CCGCAGCCATGTCCACCAGGTCCGTGTCCTCGTCCTCCTACCGCAGGATGTTCGGCGGCC CGGGCACCGCGAGCCGGCCGAGCTCCAGCCGGAGCTACGTGACTACGTCCACCCGCACCT ACAGCCTGGGCAGCGCGCTGCGCCCCAGCACCAGCCGCAGCCTCTACGCCTCGTCCCCGG GCGGCGTGTATGCCACGCGCTCCTCTGCCGTGCGCCTGCGGAGCAGCGTGCCCGGGGTGC GGCTCCTGCAGGACTCGGTGGACTTCTCGCTGGCCGACGCCATCAACACCGAGTTCAAGA ACACCCGCACCAACGAGAAGGTGGAGCTGCAGGAGCTGAATGACCGCTTCGCCAACTACA TCGACAAGGTGCGCTTCCTGGAGCAGCAGAATAAGATCCTGCTGGCCGAGCTCGAGCAGC TCAAGGGCCAAGGCAAGTCGCGCCTGGGGGACCTCTACGAGGAGGAGATGCGGGAGCTGC GCCGGCAGGTGGACCAGCTAACCAACGANCAAGCCCGCGTCGAGGTGGAGCGCGANCACC TGGNCCGAGACATCATGCGCCTNCGGGAGAAATTGCAGGANGAGATGCTTCAGAGAGAGG AAGCCGAAACACCCTGCAATCTTTCAGACAGGGATGTGACNATGCGTCTCTGGCACGTCT TGACCTTGAACGCAAAGTGGAATCTTTGCAAGAAGAGATTGCCCCTTTTGAAGAAACTCC NCCGAAAGGAAATCCAGGAGCTG >OriGene 3' read for NM_003380 unedited
ATGGACCGCGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGAGTTTTTCCAA AGATTATTGAAGCAGAACCAAGTTGGTTGGATACTTGCTGGAAAAAAAAACAGTTTTAAT GGTATTCAAAATACCTTTTAAAAAGTATTCTAGCACAAGATTTTTCTGTAAACTAGATTA TGTTGTAAACTTTTTTCTAAATCTTGTAGGAGTGTCGGTTGTTAAGAACTAGAGCTTATT CCTATTCCAAATCTATCTTGCGCTCCTGAAAAACTGCAGAAAGGCACTTGAAAGCTGTTT CTTTAAGATATGGATTTCTTTTTTATTCTTGCTGGTAATATATTGCTGCACTGAGTGTGT GCAATTTTTATTCAAGGTCATCGTGATGCTGAGAAGTTTCGTTGATAACCTGTCCATCTC TAGTTTCAACCGTCTTAATCAGAAGTGTCCTTTTTGAGTGGGTATCAACCAGAGGGAGTG AATCCAGATTAGTTTCCCTCAGGTTCAGGGAGGAAAAGTTTGGAAGAGGCAGAGAAATCC TGCTCTCCTCGCCTTCCAGCAGCTTCCTGTAGGTGGCAATCTCAATGTCAAGGGCCATCT TAACATTGAGCAGGTCTTGGTATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCT GAATCTCATCCTGCAGGCGGCCAATAGTGTCTTGGTAGTTAGCAGCTTCAACGGCAAAGT TCTCTTCCATTTCACGCATCTGGCGTTCCAGGAACTCATTGGTTCCTTTAGGGCCTCCAC TCACAGGTGAAGGACTGCACCTGTCTCCGGACTTAGTGGACTCCTGCCTTGCCTGCCGCA AGCGCGTATTGTTCCGGTTGGCAACCTAAAGAGTCAGCAACTCGGATTTGCCT |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003380 |
| Insert Size | 1401 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003380.2, NP_003371.2 |
| RefSeq Size | 1847 bp |
| RefSeq ORF | 1401 bp |
| Locus ID | 7431 |
| UniProt ID | P08670 |
| Domains | filament, filament_head |
| Protein Families | ES Cell Differentiation/IPS |
| Gene Summary | This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients. [provided by RefSeq, Aug 2017] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Proteomic Analysis Implicates Vimentin in Glioblastoma Cell Migration
,Nowicki, MO;Hayes, JL;Chiocca, EA;Lawler, SE;,
Cancers (Basel) 2019
,PubMed ID 30987208
[VIM]
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Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein
,Chu, J;Haynes, RD;Corbel, SY;Li, P;González-González, E;Burg, JS;Ataie, NJ;Lam, AJ;Cranfill, PJ;Baird, MA;Davidson, MW;Ng, HL;Garcia, KC;Contag, CH;Shen, K;Blau, HM;Lin, MZ;,
Nat. Methods
,PubMed ID 24633408
[VIM]
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Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes
,Andrew G York, Alireza Ghitani, Alipasha Vaziri, Michael W Davidson, Hari Shroff,
Nature Methods (13 February 2011) doi:10.1038/nmeth.1571 Article
[VIM]
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Identification of Novel Protein Targets for Modification by 15-Deoxy-delta-12,14-Prostaglandin J2 in Mesangial Cells Reveals Multiple Interactions with the Cytoskeleton
,Konstantinos Stamatakis, Francisco J. Sánchez-Gómez, and Dolores Pérez-Sala,
J. Am. Soc. Nephrol. 2006 Jan;17(1):89-98.
[VIM]
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201546 | VIM (Myc-DDK-tagged)-Human vimentin (VIM) |
CNY 3,656.00 |
|
| RC201546L1 | Lenti ORF clone of Human vimentin (VIM), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201546L2 | Lenti ORF clone of Human vimentin (VIM), mGFP tagged |
CNY 5,890.00 |
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| RC201546L3 | Lenti ORF clone of Human vimentin (VIM), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC201546L4 | Lenti ORF clone of Human vimentin (VIM), mGFP tagged |
CNY 6,056.00 |
|
| RG201546 | VIM (tGFP-tagged) - Human vimentin (VIM) |
CNY 5,256.00 |
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