MRPL3 (NM_007208) Human Untagged Clone
CAT#: SC111188
MRPL3 (untagged)-Human mitochondrial ribosomal protein L3 (MRPL3), nuclear gene encoding mitochondrial protein
CNY 3,656.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | COXPD9; MRL3; RPML3 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC111188 sequence for NM_007208 edited (data generated by NextGen Sequencing)
ATGCCGGGTTGGAGGCTGCTGACGCAGGTCGGCGCCCAGGTGCTGGGTCGACTCGGGGAC GGCCTGGGTGCTGCCCTGGGCCCGGGGAACAGAACACACATCTGGCTTTTTGTTAGAGGT CTTCATGGAAAGAGTGGTACATGGTGGGATGAGCATCTTTCTGAAGAAAATGTCCCATTC ATTAAGCAGTTGGTCTCTGATGAAGATAAAGCCCAATTAGCAAGTAAACTGTGTCCTCTG AAAGATGAACCATGGCCTATACATCCTTGGGAACCAGGTTCCTTTAGAGTTGGTCTTATT GCCTTGAAGCTGGGCATGATGCCTTTATGGACCAAGGATGGTCAAAAGCATGTGGTCACA TTACTTCAGGTACAAGACTGTCATGTCTTAAAATATACGTCAAAGGAAAACTGTAATGGA AAAATGGCAACCCTGTCTGTAGGAGGAAAAACTGTATCACGTTTTCGTAAAGCTACATCC ATATTGGAATTTTACCGGGAACTTGGATTGCCGCCGAAACAGACAGTTAAAATCTTTAAT ATAACAGATAATGCTGCAATTAAACCAGGCACTCCTCTTTATGCTGCTCACTTTCGTCCA GGACAGTATGTGGATGTCACAGCCAAAACTATTGGTAAAGGTTTTCAAGGTGTCATGAAA AGATGGGGATTTAAAGGCCAGCCTGCTACGCATGGTCAAACGAAAACCCACAGGAGACCT GGAGCTGTTGCAACTGGTGATATTGGCAGAGTCTGGCCTGGAACTAAAATGCCTGGAAAA ATGGGAAACATATACAGGACAGAATATGGACTGAAAGTGTGGAGAATAAACACAAAGCAC AACATAATCTATGTAAATGGCTCTGTACCTGGACATAAAAATTGCTTAGTAAAGGTCAAA GATTCTAAACTGCCTGCATATAAGGATCTCGGTAAAAATCTACCATTCCCTACATATTTT CCTGATGGAGATGAAGAGGAACTGCCAGAAGATTTGTATGATGAAAACGTGTGTCAGCCC GGTGCGCCTTCTATTACATTTGCCTAA Clone variation with respect to NM_007208.3 >OriGene 5' read for NM_007208 unedited
GGGGGGGGGCGGGGCCCACNNNCCCTTTTTTTNCCCCCCCCCGGTTCAGAATTTGTNATA CGATTACTATAGGCGGCCGCGNATATCGCACGAGGGTGGGNGACTCCCTNGAAGCAGAGC GGCAGGGCGCCCGGAAGTCGTGAGTCGAGTCTTCCCGGGCTAATCCATGCCGGGTTGGAG GCTGCTGACGCAGGTCGGCGCCCAGGTGCTGGGTCGACTCGGGGACGGCCTGGGTGCTGC CCTGGGCCCGGGGAACAGAACACACATCTGGCTTTTTGTTAGAGGTCTTCATGGAAAGAG TGGTACATGGTGGGATGAGCATCTTTCTGAAGAAAATGTCCCATTCATTAAGCAGTTGGT CTCTGATGAAGATAAAGCCCAATTAGCAAGTAAACTGTGTCCTCTGAAAGATGAACCATG GCCTATACATCCTTGGGAACCAGGTTCCTTTAGAGTTGGTCTTATTGCCTTGAAGCTGGG CATGATGCCTTTATGGACCAAGGATGGTCAAAAGCATGTGGTCACATTACTTCAGGTACA AGACTGTCATGTCTTAAAATATACGTCAAAGGAAAACTGTAATGGAAAAATGGCAACCCT GTCTGTAGGAGGAAAAACTGTATCACGTTTTCGTAAAGCTACATCCATATTGGAATTTTA CCGGGAACTTGGATTGCCGCCGAAACAGACAGTTAANATCTTTAATATAACAGATAATGC TGCAATTAAACCAGGCACTCCTCTTTATGCTGCTCACTTTCGTCCAGGACAGTATGTGGA TGTCACAGCCAAAACTATTGGTAAAGGTTTTCAGGNTGTCATGANNAGATGGGGATNTAA AGGCCAGNCTGCTACGCATGGTCAAACGAAAACCACAGGAGACCTGNAGCTGTTGCACCT GGTGATATTGGCAGAGTCTGGCCTGGNAACTAAATGCCCTGGAAAATGGGAAACATATAC >OriGene 3' read for NM_007208 unedited
CGCGGCACGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGATAATTTATGATTTTAT TGTCTTTCCTTTGTCCGGCCTTTAACATGTTTCTGTAATTTAAATAAAAATCTATTTACT TTCTCCATTTTAGCAAATGGTTTCTTTACCCAAATAGGTTGCACTATAGTCCCCATATGG TTTTCTACTGTTCCACAACCACTATTTCACAAAGATTGACAAAACTTTAATAAAAGTTAA ATTTACAGACATCTTAAGATAACTTGGGAAATATGTAGTAAAAAAGAATCGAGTCCACAA ATTAAGAATATTTTGCTAATATGCCCAACACCAATTTCAGCAAATCCAATCTACTTAACT CATATATTTAATGTGGTAATTTTTCTAACAAAATTTAATGGGGGTATGAATGATATATTT ATGCCCTTGACAAAGATGACATGTGTGATTTTGTTGTGACTAAGAAAGGAGAGTATGATT TCTGGTGGTTATGATATCACTCTGGCTCATCGAAGCTCACAGAATATGTAAGGTTCTGCC ACGTCCAAAGATGTTAGGCAAATGTAATAGAAGGCGCACCGGGCTGACACACGTTTTCAT CATACAAATCTTCTGGCAGTTCCTCTTCATCTCCATCAGGAAAATATGTAGGGAATGGTA GATTTTTACCGAGATCCTTATATGCAGGCAGTTTAGAATCTTTGACCTTTACTAAGCAAT TTTTATGTCCAGGTACAGAGCCATTTACATAGATTATGTTGNGCTTTGTGTTTATTCTCC ACACTTTCAGTCCATATTCTGTCCTGTATATGTTTCCCATTTTTNNCAGCATTNTAGTTC CCAGCCAGACTCTGNCAATATCACCAGNTGCAACAGCTNCCAGTCTNCTGTGGTNTTTCG TTTTGACATGCGTAGNCAGCTGGCCTTTAATNCCCATCTTTCATGACACCTGNNAACTTN NACATAGNNTTGCTGGN |
Restriction Sites | NotI-NotI |
ACCN | NM_007208 |
Insert Size | 1540 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_007208.2, NP_009139.1 |
RefSeq Size | 1750 bp |
RefSeq ORF | 1047 bp |
Locus ID | 11222 |
UniProt ID | P09001 |
Domains | Ribosomal_L3 |
Protein Families | Druggable Genome |
Gene Summary | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L3P ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 13q. [provided by RefSeq, Jul 2008] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC201630 | MRPL3 (Myc-DDK-tagged)-Human mitochondrial ribosomal protein L3 (MRPL3), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
|
RC201630L3 | Lenti ORF clone of Human mitochondrial ribosomal protein L3 (MRPL3), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC201630L4 | Lenti ORF clone of Human mitochondrial ribosomal protein L3 (MRPL3), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
RG201630 | MRPL3 (tGFP-tagged) - Human mitochondrial ribosomal protein L3 (MRPL3), nuclear gene encoding mitochondrial protein |
CNY 5,256.00 |
|
SC322175 | MRPL3 (untagged)-Human mitochondrial ribosomal protein L3 (MRPL3), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |