ZNF331 (NM_018555) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RITA; ZNF361; ZNF463
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC111333 sequence for NM_018555 edited (data generated by NextGen Sequencing) ATGGCCCAGGGTTTGGTGACGTTCGCCGACGTAGCCATAGACTTTTCTCAGGAGGAGTGGGCCTGTCTGA ACTCTGCTCAGAGGGACCTGTACTGGGACGTGATGCTGGAGAACTACAGTAACTTGGTCTCACTGGATTT GGAGTCAGCATATGAAAATAAGAGTTTACCTACAGAAAAAAACATTCATGAAATAAGGGCTTCCAAAAGG AATTCAGATAGAAGAAGTAAATCCCTTGGCCGTAACTGGATATGTGAAGGTACGCTTGAAAGACCACAGC GCTCCAGAGGGAGGTATGTCAATCAGATGATCATCAATTATGTCAAAAGACCTGCTACTAGAGAAGGCAC CCCTCCTAGAACACATCAGAGACATCATAAGGAGAATTCCTTTGAATGTAAGGACTGTGGGAAGGCCTTT AGTCGTGGCTATCAACTTAGTCAACATCAGAAAATCCATACTGGTGAGAAACCTTATGAATGTAAAGAAT GTAAGAAGGCCTTCCGTTGGGGCAATCAGCTTACTCAACATCAAAAAATTCATACTGGGGAGAAGCCCTA CGAATGTAAAGACTGTGGGAAGGCTTTTCGATGGGGCTCAAGCCTCGTTATTCATAAGAGGATTCATACT GGTGAAAAACCCTATGAATGTAAAGACTGTGGAAAGGCCTTTCGGCGTGGTGATGAGCTCACTCAGCACC AGAGATTCCACACTGGGGAGAAAGACTACGAATGCAAAGACTGTGGGAAGACCTTTAGCCGTGTGTATAA ACTTATTCAGCACAAGAGAATTCATAGTGGGGAGAAGCCTTACGAGTGTAAAGACTGTGGGAAGGCTTTT ATTTGTGGTTCAAGCCTCATTCAGCATAAAAGAATTCACACAGGTGAGAAACCCTATGAATGTCAAGAAT GTGGGAAGGCCTTTACTCGAGTCAATTACCTTACTCAGCATCAGAAGATCCACACCGGTGAGAAGCCTCA CGAATGTAAGGAGTGTGGGAAGGCCTTTCGCTGGGGTTCGAGCCTCGTTAAGCACGAGAGGATACATACG GGCGAGAAGCCGTACAAGTGCACAGAATGTGGGAAGGCCTTCAATTGTGGCTATCACCTCACTCAGCACG AGAGAATCCACACAGGCGAAACCCCGTATAAATGTAAGGAGTGTGGGAAGGCTTTCATTTATGGATCGAG CCTCGTGAAACATGAGAGAATTCATACCGGGGTGAAACCCTATGGGTGTACAGAATGTGGGAAGAGCTTT AGTCACGGCCATCAGCTTACACAACATCAGAAAACGCACAGTGGGGCGAAATCCTACGAATGTAAGGAGT GCGGGAAGGCATGTAACCACCTAAACCATCTCCGAGAACATCAGAGGATCCACAACAGTTGA Clone variation with respect to NM_018555.5 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for NM_018555 unedited GAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGGCTGTGACTGGTCGGG ATCGGGCCTGGATGCATGGCCTTTTGAGTGAGCACAGGTGTGTGCGACCATGTCACCTGT CTTTCTAGTGTCTCCGTTGCTTCCTGTGCCTTGGCCTTCCAGCCATGTGGTACCTGCTAG GGAGCAGTGGTCCCAAAGTGACGTGAGAGGCTCTACGCTGTTGTGAAAATGACCAAGAAG AAGGCTTCACAAGTCACGAGATGGCAGTTTTGGAGCCAGAAGTGCTCTCCGTGCACACTA CCTGGACGACAAGAGGAAGTAACCTCAAGAATAGAATTCCTTTTGGGGAAGAAAGAGCTG GCCTTCAGGAAAAGCATCCCGGAGGAGGAAGAAGAAGTGTTATATGTCCTAAAGTAAGGT TTCAGGCCAGCATCCTTCAGAAAAAGCATCCCCGAGGAGGAAGACGAATCGTTAAACATC TGAAAGGGTCAGGCCAGCATCCTTCAGAAAAAGCATCCCCGAGGAGGAAGACGAATCGTT AAACATCTTAGGTCAGCTTTACCAGTGAGGAAACCGACACTGTGAGCTCTAGCCTCTCGG AATTTGTCTTCTTCAGTGGAAACCCCGAGAGACTGATCAGTTCTTCAGTTCTAAAACAAT GGCCCAGGGTTTGGTGACGTTCGCCGACGTAGCCATAGACTTTTCTCAGGAGGAGTGGGC CTGTCTGAACTCTGCTCAGAGGGACCTGTACTGNGACGTGATGCTGGAGAACTACAGNTA CTGGNTCTCACTGGATTTGGAGTCAGCTATGAAANTAGAGTTTACCTACAGAAAAACATT CTGAAATAAGGGCTTCAAAGGAATCAATAGAGAATAATCCCTGNCCGACTGGAAGTGAAG TACCTGAAGACCACGCTCG 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for NM_018555 unedited CGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTAAGTTGAGAACTTTCACC TTTTCATTTAAAAGGAAGCACTTTGTGGCTTCTCTTTGGCATATCCGAATCACCAGCATC ACTACTCCTGCTCTCTGGGGCCACTGTTAAGCAAAGTGAGGACTGCTTGGGCACAGGCAC TGTGATGCTGGGATAGTTGATCTGATCACCAAGACGGCTACTAAGTCACTAGCAGGGTGG GTGGCGTATACAGCGTGGATGTGCTGGACCAAGGGATGACTCACATCCCCGGCCGGCTGG AGCCGGACAGCGAGAGATTTCATCACGCTACTCAGAAGGGCACACCATTTGAGACTTAAA ATTCTTTATTTCTGGAATTTTCCATTTAATATTTTTGAACTGCAGTTGACTGCAGGTAAC AAACTGTGGAAAGCGAAACCATAGATACGAGCGGGCTACTGCGTTCAAAAGGCTCTTCAA CTGTTGTGGATCCTCTGATGTTCTCGGAGATGGTTTAAGTGGTTACATGCCTTCCCGCAC TCCTTACATTCGTAGGATTTCGCCCCACTGTGCGTTTTCTGATGTTGTGTAAGCTGATGG CCGTGACTAAAGCTCTTCCCACATTCTGTACACCCATAGGGTTTCACCCCCGTATGACTT TTCTCATTGTTTCACGAGGCTTCGACCCATTAATGAAAACCTTCCCACACTCCTTACATT TATACCGGGGTTTCGCCCTGTGTGGATCCTTCTCGGGCTTGAGTGATGGGGATAGCCACC AATGGAAGGCCCTCCCACATTTCGGGCACTTGTACCGGTTTTTCGCCCGTATGTATCCTC TTTGGGCTTACGAGGGTCGAACCCCCACGGAAGAGCCCTTCCCACCTCCCTTAACTTCCT GGAGCTTTCTAACCGGGGGCGGACTTCTTATGCTGGAAAAAGAATCTGATTTTGATAAAG GCCTCTCCACTTTTTTACAATATT
Restriction Sites	NotI-NotI
ACCN	NM_018555
Insert Size	2570 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_018555.4, NP_061025.4
RefSeq Size	5163 bp
RefSeq ORF	1392 bp
Locus ID	55422
UniProt ID	Q9NQX6
Domains	KRAB, zf-C2H2
Protein Families	Transcription Factors
Gene Summary	This gene encodes a zinc finger protein containing a KRAB (Kruppel-associated box) domain found in transcriptional repressors. This gene may be methylated and silenced in cancer cells. This gene is located within a differentially methylated region (DMR) and shows allele-specific expression in placenta. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding the same protein. [provided by RefSeq, Nov 2015] Transcript Variant: This variant (1, also known as g) represents the longest transcript. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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