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PRAF1 (POLR1E) (NM_022490) Human Untagged Clone
CAT＃: SC111400
POLR1E (untagged)-Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | A49; PAF53; PRAF1; RPA49 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_022490, the custom clone sequence may differ by one or more nucleotides
ATGGCGGCGGAGGTGTTGCCGAGTGCGAGGTGGCAGTATTGTGGGGCGCCCGACGGGAGCCAGAGAGCTG TACTGGTCCAGTTCTCCAACGGGAAGCTACAGAGTCCAGGCAACATGCGCTTTACCTTGTATGAGAACAA AGATTCCACCAACCCCAGGAAGAGGAATCAACGGATCCTGGCAGCTGAAACAGATAGGCTCTCCTATGTG GGAAACAATTTTGGGACTGGAGCCCTCAAATGCAACACTTTGTGCAGGCACTTTGTGGGAATTTTGAACA AGACCTCTGGCCAAATGGAAGTATATGATGCTGAATTGTTCAACATGCAGCCACTATTTTCAGATGTATC AGTTGAGAGTGAACTGGCGCTAGAGAGTCAGACCAAAACTTACAGAGAAAAGATGGATTCTTGTATTGAA GCCTTTGGTACCACCAAACAGAAGCGAGCTCTGAACACCAGGAGAATGAACAGAGTTGGCAATGAATCTT TGAATCGTGCAGTGGCTAAAGCTGCAGAGACTATCATTGATACGAAGGGTGTGACTGCTCTGGTCAGCGA TGCTATCCACAATGACTTGCAAGATGACTCCCTCTACCTTCCTCCCTGCTATGATGATGCAGCCAAGCCT GAAGACGTGTATAAATTTGAAGATCTTCTTTCCCCTGCGGAGTATGAAGCTCTTCAGAGCCCATCTGAAG CTTTCAGGAACGTCACGTCAGAAGAAATACTGAAGATGATTGAGGAGAACAGCCATTGCACCTTTGTCAT AGAAGCGTTGAAGTCTTTGCCATCAGATGTGGAGAGCCGAGACCGCCAGGCCCGATGCATATGGTTTCTG GATACCCTCATCAAATTTCGAGCTCATAGGGTAGTTAAGCGGAAAAGTGCTCTGGGACCTGGAGTTCCCC ACATCATCAACACCAAACTGCTGAAGCACTTTACTTGCTTGACCTACAACAATGGCAGATTACGGAACTT AATTTCGGATTCTATGAAGGCGAAGATTACTGCATATGTGATCATACTTGCCTTGCACATACATGACTTC CAAATTGACCTGACAGTGTTACAGAGGGACTTGAAGCTCAGTGAGAAAAGGATGATGGAGATAGCCAAAG CCATGAGGCTGAAGATCTCCAAAAGAAGGGTGTCTGTGGCCGCCGGCAGTGAAGAAGATCACAAACTGGG CACCCTGTCCCTCCCGCTGCCTCCAGCCCAGACCTCAGACCGCCTGGCAAAGCGGAGGAAGATTACCTAG >OriGene 5' read for NM_022490 unedited
GCCGTTCGGGATTTGTATACGATTTACTATAGGCGGCCGCGCAATTCGCACGAGGCTCGG CCTGGGCTCCCGCGTGTTTAAAATGCGCTTGTGGCTGCTGCTGTCTTAACTCCTGTGCTT GGCGGACAGACAGGCGAGATGGCGGCGGAGGTGTTGCCGAGTGCGAGGTGGCAGTATTGT GGGGCGCCCGACGGGAGCCACAGAGCTACTCTGGTCCAGTTCTCCAACGGGAAGCTACAG AGTCCAGGCAACATGCGCTTTACCTTGTATGAGAACAAAGATTCCACCAACCCCAGGAAG AGGAATCAACGGATCCTGGCAGCTGAAACAGATAGGCTCTCCTATGTGGGAAACAATTTT GGGACTGGAGCCCTCAAATGCAACACTTTGTGCAGGCACTTTGTGGGAATTTTGAACAAG ACCTCTACCCAAATGGAAGTATATGATGCTGAATTGTTCAATATGCTGCCACTATTTTCA GATGTATCAGTTGAGAGTGAACTGGCGCTAGAGAGTCAGACCAAAACTTACAGAGAAAAG ATGGATTCTTGTATTGAAGCCTTTGGTACCACCAAACAGAAGCGAGCTCTGAACACCAGG AGAATGAACAGAGTTGGCAATGAATCTTTGAATCGTGCAGTGGCTAAAGCTGCAGAGACT ATCATTGATACGAAGGGTGTGACTGCTCTGGTCACCGATGCTTTCCCAATGACTTGCAAG ATGACTCCCTCTACCTTCCTNCCTGCTATGATGATGCACCCAAGCCTGAATACGTGTTTA AATTGTGAAATCTTTTTTTCCCTGCGGAGTATGAAACTCTTTCAAAACGCATCTGAAGCT TTCACGAACCTCCCGCCAAAAGAATACTGGACATAATGGGGAGAACAGCCTTTGCCCCT >OriGene 3' read for NM_022490 unedited
CCCTCACTTGCTANAGTCGAGAAATTCTTTTTTTTTTTTGAAGTCCAACCTTTCCATCGC TTTATATTTCTGCATAAGACAAGCACAGTTGAGATGCTGGCTTCTGGCTTCATACCTGTG CTAAAGCAAGGCTTCTTTTTTCCTTATGTTACTTTTTTGAGACAAGGTCTCACTCTGTGA CCCAGGATGGAGTGTGGCCACTCATCTTAGCTCACTGCAACCTTGATTTCCCAAGCTCAA GTAATCGTCCTGCCTCAGCCTCCCGAGTAGCTGGGATCACAGGCGCACACCACCAGGCCT GACTCTTTTTTTTTTCCTCCGGTAGAGATGGGGTCTCCCTATGTTGTCCAGACTGGTTTC AAACTCCTGAGCTCAATGATCTTCCTGCCTCGGCCTCCCAAAGTGCTGGGATTTCAGGTG TGAGCCACCATCCCCGGACCTTTTCTTTTCAAAACATACATAAAAATGGAAATGAATAGG ACCAGCCAGTGGCTGTGATGCAGCCAAAACGCCCTGTCTGGAAAGCATGCGTCTAGGTAA TCTTCCTCCGCTTCGCCAGGCGGTCTGACGTCTGGGCTGGAGGCACCGGGAGGGACAGGG TGCCCAGTTTGTGATCTTCTCTCACTGCCGGCGGCCACAGACACCCTTCTTTTGGAGATC TTCAGCCTCATGGCTTTGGCTATCTCCATCATCCTTTTCTCACTGAGCTTCAAGTCCCTC TGTAACACTGCCAGGTCAATTTGGAAGTCATGCATGTGCCAGGCAAGGATGATCACATAT GCAGCGATTCTCCGCCTTCATAGACACCCGAAATTAAGTTCCGTACTCTGCCATTGTTGT AAGGCAAGCACGCAAAGTGCTTCCACAGCTCCGGTGCTGCTGATGTGCGGAACTCCAGGC CCAGCACCCACTTTCCCTTATAAACCCTACGACCCGAACTTGCCGAGGGGTCCACGAACC TTGCATCGGCGCTGCGGCTTCGGCTCCCACATCGAGGG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_022490 |
| Insert Size | 1790 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_022490.1, NP_071935.1 |
| RefSeq Size | 1854 bp |
| RefSeq ORF | 1260 bp |
| Locus ID | 64425 |
| UniProt ID | Q9GZS1 |
| Protein Families | Transcription Factors |
| Protein Pathways | Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204811 | POLR1E (Myc-DDK-tagged)-Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E) |
CNY 3,656.00 |
|
| RC204811L1 | Lenti ORF clone of Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC204811L2 | Lenti ORF clone of Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E), mGFP tagged |
CNY 5,890.00 |
|
| RC204811L3 | Lenti ORF clone of Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC204811L4 | Lenti ORF clone of Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E), mGFP tagged |
CNY 5,890.00 |
|
| RG204811 | POLR1E (tGFP-tagged) - Human polymerase (RNA) I polypeptide E, 53kDa (POLR1E) |
CNY 5,256.00 |
